{"id":84674,"date":"2021-08-30T08:29:16","date_gmt":"2021-08-30T08:29:16","guid":{"rendered":"http:\/\/pingtungtimes.com.tw\/?p=84674"},"modified":"2021-08-30T08:29:18","modified_gmt":"2021-08-30T08:29:18","slug":"12%e5%80%8b%e7%a0%94%e7%99%bc%e6%8a%97%e6%af%92%e8%97%a5%e7%89%a9%e5%bf%83%e5%be%97%e8%88%87%e8%ab%b8%e5%8f%8b%e5%88%86%e4%ba%ab","status":"publish","type":"post","link":"https:\/\/pingtungtimes.com.tw\/?p=84674","title":{"rendered":"12\u500b\u7814\u767c\u6297\u6bd2\u85e5\u7269\u5fc3\u5f97\u8207\u8af8\u53cb\u5206\u4eab"},"content":{"rendered":"\n

\u3010\u674e\u91cd\u5fb7\uff0f\u5c08\u6b04\u5831\u5c0e2021\/08\/27\u3011\u3000\u91cd\u5fb7\u7814\u8b80\u4e86\u5e7e\u7bc7\u6709\u95dc\u92c5\u96e2\u5b50\u6291\u5236\u51a0\u72c0\u75c5\u6bd2\u7684\u8ad6\u6587\u4e4b\u5f8c\uff0c\u5df2\u7d93\u7565\u6709\u5fc3\u5f97\u4e86\uff0c\u7576\u7136\uff0c\u9019\u4e9b\u8cc7\u6599\u5c0d\u65bc\u4e00\u500b\u975e\u6b64\u9818\u57df\u7684\u4eba\u4f86\u8aaa\u90fd\u662f\u975e\u5e38\u5de8\u91cf\u800c\u4e14\u7d22\u7136\u7121\u5473\u7684\uff0c\u4f46\u662f\u91cd\u5fb7\u60f3\u8981\u63d0\u7db1\u6308\u9818\u7684\u628a\u5e7e\u500b\u95dc\u9375\u6027\u7684\u91cd\u9ede\u63d0\u51fa\u4f86\u548c\u5404\u4f4d\u597d\u53cb\u8a0e\u8ad6\u8207\u5206\u4eab\uff0c\u56e0\u70ba\u9019\u662f\u5927\u5bb6\u4ea4\u4e86\n\u91cd\u5fb7 \u9019\u6a23\u4e00\u500b\u52c7\u65bc\u627f\u64d4\u5927\u4efb\u8005\u7684\u4e00\u500b\u201c\u9810\u77e5\u798f\u5229\u201d\u3002<\/p>\n\n\n\n

\u4ee5\u4e0b\u5c31\u662f\u91cd\u5fb7\u6700\u8fd1\u7684\u4e00\u4e9b\u5fc3\u5f97\uff0c\u8207\u5927\u5bb6\u5171\u540c\u8a0e\u8ad6\u4e4b\uff1a\u3010\u8a0e\u8ad601\u3011\n\u6b63\u93c8RNA\u75c5\u6bd2\u5982\u4f55\u5728\u7d30\u80de\u5167\u9032\u884c\u8907\u88fd\u5462\uff1f\u4e00\u822c\u4f86\u8aaa\u751f\u7269\u5b78\u5bb6\u662f\u6839\u64daRNA\u80fd\u5426\u76f4\u63a5\u8d77mRNA\u4f5c\u7528\u800c\u5206\u6210\u55ae\u80a1\u6b63\u93c8RNA\u75c5\u6bd2(\u6b63\u80a1RNA\u75c5\u6bd2)\u8207\u55ae\u80a1\u8ca0\u93c8RNA\u75c5\u6bd2(\u8ca0\u80a1RNA\u75c5\u6bd2)\u5169\u7a2e\u3002<\/p>\n\n\n\n

(1)\u6b63\u80a1RNA\u75c5\u6bd2\uff08\u5982\u810a\u9ad3\u7070\u8cea\u708e\u75c5\u6bd2\uff09\u7684\u55ae\u80a1RNA\u53ef\u76f4\u63a5\u8d77mRNA\u4f5c\u7528\uff0c\u8f49\u8b6f\u65e9\u671f\u86cb\u767d\u8cea\uff0c\u5305\u62ecRNA\u591a\u805a\u9176\u548c\u6291\u5236\u5bbf\u4e3b\u7d30\u80de\u5408\u6210\u4ee3\u8b1d\u7684\u8abf\u63a7\u86cb\u767d\u3002\u7136\u5f8c\u5728RNA\u591a\u805a\u9176\u7684\u4f5c\u7528\u4e0b\uff0c\u4ee5\u55ae\u80a1RNA\uff08\u6b63\u80a1\uff09\u70ba\u6a21\u677f\uff0c\u5f62\u6210\u4e92\u88dc\u80a1\uff08\u8ca0\u80a1\uff09\uff0c\u5169\u8005\u7d50\u5408\u6210\u96d9\u80a1\uff0c\u7a31\u70ba\u8907\u88fd\u578b\u3002\u518d\u7531\u8ca0\u80a1\u7522\u751f\u51fa\u8a31\u591a\u65b0\u7684RNA\uff08\u6b63\u80a1\uff09\uff0c\u6210\u70ba\u8907\u88fd\u4e2d\u9593\u9ad4\u3002\u6b64\u65b0\u7684\u6b63\u80a1RNA\u53ef\u4f5c\u70bamRNA\u5408\u6210\u8863\u6bbc\u86cb\u767d\u3002<\/p>\n\n\n\n

(2)\u8ca0\u80a1RNA\u75c5\u6bd2\uff08\u5982\u6b63\u7c98\u75c5\u6bd2\u3001\u526f\u7c98\u75c5\u6bd2\u3001\u5f48\u72c0\u75c5\u6bd2\uff09\u7684\u55ae\u80a1RNA\u4e0d\u80fd\u4f5c\u70bamRNA\uff0c\u7a31\u70ba\u8ca0\u80a1\uff0c\u9808\u5148\u5408\u6210\u4e92\u88dc\u80a1\uff08\u6b63\u80a1\uff09\u4f5c\u70bamRNA\uff0c\u518d\u8f49\u8b6f\u86cb\u767d\u5206\u5b50\uff0c\u800c\u5f8c\u7522\u751f\u6838\u9178\u7684\u8907\u88fd\u578b\uff0c\u6210\u70ba\u5408\u6210\u5b50\u4ee3\u75c5\u6bd2RNA\u7684\u6a21\u677f\u3002<\/p>\n\n\n\n

\u56e0\u6b64\uff0c\u6b63\u93c8RNA\u75c5\u6bd2\u7684\u57fa\u56e0\u7d44\u70ba\u4e00\u689d\u6b63\u80a1\u7684\u55ae\u93c8RNA\uff0c\u75c5\u6bd2\u9ad4\u672c\u8eab\u4e0d\u651c\u5e36RNA\u8907\u88fd\u9176\u3002<\/p>\n\n\n\n

\u5176\u907a\u50b3\u4fe1\u606f\u50b3\u905e\u904e\u7a0b\u5206\u6210\u4ee5\u4e0b\u5e7e\u7a2e\u65b9\u5f0f\uff1a<\/p>\n\n\n\n

(1).\u6b63\u93c8RNA\u9032\u5165\u5bbf\u4e3b\u7d30\u80de\u5f8c\uff0c\u4e00\u4e9b\u76f4\u63a5\u4f5c\u70bamRNA\u93c8\u6307\u5c0e\u86cb\u767d\u8cea\u7684\u5408\u6210\uff1b\u9019\u4e9b\u86cb\u767d\u8cea\u7522\u7269\u6709\u4f9d\u8cf4RNA\u7684RNA\u805a\u5408\u9176(RdRp)\u53ca\u8863\u6bbc\u86cb\u767d\u3002<\/p>\n\n\n\n

(2).\u53e6\u4e00\u4e9b\u6b63\u93c8RNA\u53ef\u4ee5\u901a\u904e\u6b64RdRp\u9176\u7684\u4f5c\u7528\uff0c\u751f\u6210\u8ca0\u93c8\uff0c\u5f62\u6210\u96d9\u93c8\u5f62\u5f0f\u7684\u8907\u88fd\u578b\u4e2d\u9593\u9ad4\uff1b\u63a5\u8457\u6b64\u96d9\u93c8\u53ef\u4ee5\u89e3\u93c8\uff0c\u518d\u4ee5\u8ca0\u93c8\u70ba\u6a21\u677f\uff0c\u5728RdRp\u4f5c\u7528\u4e0b\uff0c\u751f\u6210\u5927\u91cf\u65b0\u7684\u6b63\u93c8RNA\uff0c\u9054\u5230\u8907\u88fd\u7684\u76ee\u7684\u3002 <\/p>\n\n\n\n

(3).\u751f\u6210\u7684\u6b63\u93c8\u53ef\u4f5c\u70bamRNA\uff0c\u6307\u5c0e\u86cb\u767d\u8cea\u7684\u5408\u6210\u6216\u4f5c\u70ba\u75c5\u6bd2\u57fa\u56e0\u7d44\u3002 <\/p>\n\n\n\n

(4).\u8863\u6bbc\u86cb\u767d\u5305\u88f9\u6b63\u93c8RNA\uff0c\u7d44\u88dd\u5f62\u6210\u5b50\u4ee3\u75c5\u6bd2\u3002<\/p>\n\n\n\n

\u3010\u8a0e\u8ad602\u3011 \u6b63\u93c8RNA\u75c5\u6bd2\u5305\u542b\u54ea\u4e00\u4e9b\u75c5\u6bd2\u7a2e\u985e\u5462\uff1f<\/p>\n\n\n\n

(1). \u7db2\u5de2\u75c5\u6bd2\u76ee\uff08Nidovirales\uff09<\/p>\n\n\n\n

\u5305\u542b\uff1b\u52d5\u8108\u708e\u75c5\u6bd2\u79d1\uff08Arteriviridae\uff09\u3001\u51a0\u72c0\u75c5\u6bd2\u79d1\uff08Coronaviridae\uff09\u3001\u6d77\u6d0b\u75c5\u6bd2\u79d1\uff08Mesoniviridae\uff09\u3001\u687f\u72c0\u5957\u75c5\u6bd2\u79d1\uff08Roniviridae\uff09\u3002<\/p>\n\n\n\n

(2). \u5c0f\u6838\u7cd6\u6838\u9178\u76ee\uff08Picornavirales\uff09<\/p>\n\n\n\n

\u5305\u542b\uff1a\u4e8c\u9806\u53cd\u5b50\u75c5\u6bd2\u79d1\uff08Dicistroviridae\uff09\u3001\u50b3\u67d3\u6027\u8edf\u5316\u75c5\u6bd2\u79d1\uff08Iflaviridae\uff09\u3001\u6d77\u6d0bRNA\u75c5\u6bd2\u79d1\uff08Marnaviridae\uff09\u3001\u5fae\u5c0f\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u79d1\uff08Picornaviridae\uff09\u3001\u4f34\u751f\u8c47\u8c46\u75c5\u6bd2\u79d1(Secoviridae)\u3001\u8c47\u8c46\u82b1\u8449\u75c5\u6bd2\u4e9e\u79d1\uff08Comoviridae\uff09\u3001Bacillarnavirus\u3001Labyrnavirus\u3002<\/p>\n\n\n\n

(3). \u856a\u83c1\u9ec3\u82b1\u8449\u75c5\u6bd2\u76ee(Tymovirales)<\/p>\n\n\n\n

\u5305\u542b\uff1a\u7532\u578b\u7dda\u5f62\u75c5\u6bd2\u79d1\uff08Alphaflexiviridae\uff09\u3001\u4e59\u578b\u7dda\u5f62\u75c5\u6bd2\u79d1(Betaflexiviridae)\u3001\u4e19\u578b\u7dda\u5f62\u75c5\u6bd2\u79d1(Gammaflexiviridae)\u3001\u856a\u83c1\u767c\u9ec3\u9472\u5d4c\u75c5\u6bd2\u79d1\uff08Tymoviridae\uff09\u3002<\/p>\n\n\n\n

(4). \u672a\u8a2d\u76ee\u7684\u75c5\u6bd2\u79d1<\/p>\n\n\n\n

\u5305\u542b\uff1aAlphatetraviridae\u3001\u8702\u7aa9\u72c0\u75c5\u6bd2\u79d1\uff08Alvernaviridae\uff09\u3001\u661f\u72c0\u75c5\u6bd2\u79d1\uff08Astroviridae\uff09\u3001\u687f\u83cc\u72c0\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u79d1\uff08Barnaviridae\uff09\u3001\u751c\u83dc\u58de\u6b7b\u9ec3\u8108\u75c5\u6bd2\u79d1\uff08Benyviridae\uff09\u3001\u96c0\u9ea5\u82b1\u8449\u75c5\u6bd2\u79d1\uff08Bromoviridae\uff09\u3001\u676f\u72c0\u75c5\u6bd2\u79d1\uff08Caliciviridae\uff09\uff1a\u5d4c\u676f\u75c5\u6bd2\u79d1\uff08Carmotetraviridae\uff09\u3001\u4fee\u9053\u9662\u75c5\u6bd2\u79d1\uff08Closteroviridae\uff09\u3001\u9ec3\u75c5\u6bd2\u79d1\uff08Flaviviridae\uff09\u3001\u809d\u708e\u75c5\u6bd2\u79d1\uff08Hepeviridae\uff09\u3001\u4f4e\u6bd2\u6027\u75c5\u6bd2\u79d1\uff08Hypoviridae\uff09\u3001\u5149\u6ed1\u75c5\u6bd2\u79d1\uff08Leviviridae\uff09\u3001\u9ec3\u75c7\u75c5\u6bd2\u79d1\uff08Luteoviridae\uff09\u3001\u88f8\u9732\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\uff08Narnaviridae)\u3001\u91ce\u7530\u75c5\u6bd2\u79d1\uff08Nodaviridae\uff09\u3001\u6d3e\u6c0f\u75c5\u6bd2\u79d1\uff08Permutotetraviridae\uff09\u3001\u99ac\u9234\u85afY\u75c5\u6bd2\u79d1\uff08Potyviridae\uff09\u3001\u62ab\u8863\u75c5\u6bd2\u79d1\uff08Togaviridae\uff09\u3001\u756a\u8304\u53e2\u77ee\u75c5\u6bd2\u79d1\uff08Tombusviridae\uff09\u3001\u5e1a\u72c0\u75c5\u6bd2\u79d1\uff08Virgaviridae\uff09\u7b49\u3002<\/p>\n\n\n\n

\u3010\u8a0e\u8ad603\u3011 \u5728\u6b63\u93c8RNA\u75c5\u6bd2\u7684\u591a\u7a2e\u8907\u5236\u7b56\u7565\u4e2d\u5c45\u95dc\u9375\u56e0\u7d20\u8005\u70ba\u4f55\uff1f<\/p>\n\n\n\n

\u6b63\u93c8RNA (+RNA) \u75c5\u6bd2\u5305\u62ec\u8a31\u591a\u91cd\u8981\u7684\u75c5\u539f\u9ad4\u3002\u5b83\u5011\u5df2\u7d93\u9032\u5316\u51fa\u591a\u7a2e\u8907\u5236\u7b56\u7565\uff0c\u4f46\u7d71\u4e00\u7684\u4e8b\u5be6\u662f\u4f9d\u8cf4\u65bc\u7d30\u80de\u5167RNA\u5df2\u5b58\u5728\u7684RNA\u805a\u5408\u9176(RdRps)\u4f5c\u70ba\u75c5\u6bd2RNA\u5408\u6210\u6a5f\u5236\u7684\u6838\u5fc3\u9176\u3002\u800c\u4e14RdRp \u901a\u5e38\u5d4c\u5165\u5728\u7531\u75c5\u6bd2 RNA\u3001\u75c5\u6bd2\u548c\u5bbf\u4e3b\u86cb\u767d\u7d44\u88dd\u800c\u6210\u7684\u819c\u76f8\u95dc\u8907\u88fd\u8907\u5408\u7269\u4e2d\u3002\u56e0\u6b64\uff0c\u6709\u6548\u7684\u6291\u5236RdRps \u662f\u6297\u75c5\u6bd2\u7814\u7a76\u7684\u95dc\u9375\u76ee\u6a19\u3002\u3008View Article\u3009\u3002<\/p>\n\n\n\n

\u91cd\u5fb7\u8a8d\u70ba\u7531\u65bc\u6b63\u93c8RNA (+RNA) \u75c5\u6bd2\u4e26\u4e0d\u81ea\u5df1\u64c1\u6709RNA\u8907\u88fd\u9176\uff0c\u56e0\u6b64\u5b83\u53ea\u80fd\u5728\u9032\u5165\u7d30\u80de\u5f8c\u5229\u7528\u7d30\u80de\u5167\u539f\u6709\u7684RNA RNA\u8907\u88fd\u9176\u4f86\u4fc3\u9032\u75c5\u6bd2\u7684\u8907\u88fd\uff0c\u800c\u76ee\u524d\u5927\u591a\u6578\u5c0d\u6297COVID-19\u7684\u75ab\u82d7\u8a2d\u8a08\u90fd\u662f\u4fc3\u4f7f\u4eba\u9ad4\u7684\u514d\u75ab\u7cfb\u7d71\u5c0d\u65bcCOVID-19\u75c5\u6bd2\u6709\u201c\u53d7\u50b7\u7d93\u9a57\u201d\uff0c\u800c\u4fc3\u4f7f\u7d30\u80de\u5728\u9047\u5230\u5916\u578b\u8207COVID-19\u75c5\u6bd2\u96f7\u540c\u7684\u5916\u4f86\u8005\u6642\u201c\u9589\u9580\u8b1d\u5ba2\u201d\u3002<\/p>\n\n\n\n

\u91cd\u5fb7\u8a8d\u70ba\u9019\u6a23\u7684\u75ab\u82d7\u8a2d\u8a08\u6703\u7522\u751f\u4e86\u4ee5\u4e0b\u5e7e\u500b\u76f2\u5340\uff1a<\/p>\n\n\n\n

\u3008\u7b2c\u4e00\u500b\u76f2\u5340\u3009\u662f\u8981\u8b93\u4eba\u9ad4\u514d\u75ab\u7cfb\u7d71\u6709\u53d7\u50b7\u7d93\u9a57\u7684\u523a\u6fc0\u65b9\u6cd5\u3001\u65bd\u52a0\u5291\u91cf\u3001\u50b7\u5bb3\u5f37\u5ea6\u3001\u6709\u6548\u671f\u9593\u3001\u5b89\u5168\u8003\u91cf\u90fd\u662f\u4e00\u500b\u5927\u8003\u9a57\u3002<\/p>\n\n\n\n

\u3008\u7b2c\u4e8c\u500b\u76f2\u5340\u3009\u662f\u4eba\u9ad4\u7684\u514d\u75ab\u7cfb\u7d71\u4e0b\u4ee4\u7d30\u80de\u201c\u9589\u9580\u8b1d\u5ba2\u201d\u7684\u53ef\u9760\u6027\u3001\u6301\u4e45\u6027\u3001\u5f8c\u907a\u75c7\u7b49\u7b49\u554f\u984c\u90fd\u5fc5\u9808\u91dd\u5c0d\u4e0d\u540c\u4eba\u7fa4\u52a0\u4ee5\u8a08\u91cf\u8a55\u4f30\u3002<\/p>\n\n\n\n

\u3008\u7b2c\u4e09\u500b\u76f2\u5340\u3009\u662fCOVID-19\u75c5\u6bd2\u5177\u6709\u9ad8\u8b8a\u7570\u7279\u6027\u8207\u667a\u6167\u578b\u9032\u5316\u529f\u80fd\uff0c\u56e0\u6b64\u75ab\u82d7\u7684\u7814\u88fd\u53ef\u80fd\u8ddf\u4e0d\u4e0a\u75c5\u6bd2\u7684\u8b8a\u7570\uff0c\u800c\u4e14\u75c5\u6bd2\u5f88\u53ef\u80fd\u6703\u91dd\u5c0d\u75ab\u82d7\u7684\u5c0d\u6297\u6a21\u5f0f\u800c\u9032\u5316\u4ee5\u8d85\u8d8a\u4e4b\u3002<\/p>\n\n\n\n

\u91cd\u5fb7\u91dd\u5c0dCOVID-19\u7684\u8a6d\u8b4e\u591a\u8b8a\u7684\u7279\u6027\uff0c\u8a8d\u70ba\u76ee\u524dCOVID-19\u75ab\u82d7\u653f\u7b56\u662f\u4e00\u500b\u6e90\u81ea\u65bc\u904e\u53bb\u201c\u4eba\u5b9a\u52dd\u5929\u201d\u7684\u5047\u8a2d\u800c\u767c\u5c55\u51fa\u201c\u4ee5\u8eab\u8a66\u6bd2\u201d\u7684\u932f\u8aa4\u91ab\u7642\u653f\u7b56\u3002<\/p>\n\n\n\n

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\u6211\u5011\u8a55\u4f30\u4e86\u5728 0 \u5230 8 \u00b5M ZnOAc2 \u5b58\u5728\u4e0b\u4e00\u7cfb\u5217 PT \u6fc3\u5ea6 (0\u201332 \u00b5M) \u7684\u7d30\u80de\u6bd2\u6027\u3002\u767c\u73fe\u7576 ZnOAc2 \u7684\u6fc3\u5ea6\u5c0f\u65bc 4 \u00b5M \u6642\uff0c\u6574\u9ad4\u7d30\u80de\u86cb\u767d\u8cea\u5408\u6210\u6c92\u6709\u8b8a\u5316\uff08\u6578\u64da\u672a\u986f\u793a\uff09\u3002\u3008View Article\u3009<\/p>\n\n\n\n

\u6211\u5011\u6e2c\u8a66\u4e86 Zn2+ \u6dfb\u52a0\u5c0d RTC \u6d3b\u6027\u7684\u5f71\u97ff\u3002\u5c0d\u65bc EAV\u548c\nSARS-CoV\uff0c\u7576\u5b58\u5728 ZnOAc2 \u6642\uff0c\u89c0\u5bdf\u5230\u5408\u6210\u7684\nRNA \u91cf\u5448\u5291\u91cf\u4f9d\u8cf4\u6027\u4e0b\u964d\u3002\u5c0d\u65bc\u9019\u5169\u7a2e\u75c5\u6bd2\uff0c\u5728 50 \u00b5M \u7684\nZn2+ \u6fc3\u5ea6\u4e0b\u89c0\u5bdf\u5230\u7e3d RNA \u5408\u6210\u6e1b\u5c11\u4e86 50% \u4ee5\u4e0a\uff0c\u800c\u5728 500 \u00b5M \u7684 Zn2+ \u6fc3\u5ea6\u4e0b\u4ecd\u4fdd\u6301\u4e0d\u5230 5% \u7684\u6d3b\u6027\u3002\u57fa\u56e0\u7d44\u5408\u6210\u548c sg mRNA \u7522\u751f\u540c\u6a23\u53d7\u5230\u5f71\u97ff\u3002\u3008View Article\u3009<\/p>\n\n\n\n

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1. \u91dd\u5c0d\u5c0f\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u9032\u884c\u4e86\u5408\u7406\u8a73\u7d30\u7684\u7814\u7a76\u5f8c\u8b49\u660e\u5728\u7d30\u80de\u57f9\u990a\u4e2d\uff0cPT\n\u5728\u6578\u5206\u9418\u5167\u523a\u6fc0Zn2+\u651d\u53d6\u4e26\u901a\u904e\u4e00\u7a2e\u6a5f\u5236\u6291\u5236 RNA \u75c5\u6bd2\u8907\u88fd\u3002\u3008View Article\u3009\u3008View Article\u3009<\/p>\n\n\n\n

2. \u5c0d\u7d14\u5316\u7684\u9f3b\u75c5\u6bd2\u548c\u810a\u9ad3\u7070\u8cea\u708e\u75c5\u6bd2 3C \u86cb\u767d\u9176\u9032\u884c\u7684\u9ad4\u5916\u7814\u7a76\u8868\u660e\uff0c\u86cb\u767d\u9176\u6d3b\u6027\u88ab Zn2+ \u6291\u5236\u3002\u3008View Article\u3009\u3008View Article\u3009<\/p>\n\n\n\n

3. \u5728\u611f\u67d3\u4eba\u9f3b\u75c5\u6bd2\u548c\u67ef\u85a9\u5947\u75c5\u6bd2B3\u7684\u7d30\u80de\u4e2d\u89c0\u5bdf\u5230\u7684\u92c5\u96e2\u5b50\u5c0d\u591a\u86cb\u767d\u52a0\u5de5\u7684\u6291\u88fd\u4f5c\u7528\u3002\u3008View Article\u3009\u3008View Article\u3009<\/p>\n\n\n\n

4. Zn2+\u5c0d\u4f86\u81ea\u9f3b\u75c5\u6bd2\u548c\u4e19\u578b\u809d\u708e\u75c5\u6bd2\u7684\u7d14\u5316RdRps\u7684\u6d3b\u6027\u6709\u6291\u88fd\u4f5c\u7528\u3002\u3008View Article\u3009\u3008View Article\u3009<\/p>\n\n\n\n

5. \u92c5\u96e2\u5b50\u88ab\u8b49\u660e\u53ef\u4ee5\u6291\u5236\u611f\u67d3\u7d30\u80de\u548c\u7121\u7d30\u80de\u7cfb\u7d71\u4e2d\u51a0\u72c0\u75c5\u6bd2\u8907\u88fd\u9176\u591a\u86cb\u767d\u52a0\u5de5\u904e\u7a0b\u4e2d\u7684\u67d0\u4e9b\u86cb\u767d\u6c34\u89e3\u88c2\u89e3\u3002\u3008View Article\u3009\u3008View Article\u3009<\/p>\n\n\n\n

\u3010\u8a0e\u8ad608\u3011 \u5728\u7f3a\u5c11\u5421\u5576\u786b\u916e(PT)\u60c5\u6cc1\u4e0b\u92c5\u96e2\u5b50\u80fd\u6291\u5236\u75c5\u6bd2\u8907\u88fd\u55ce\uff1f<\/p>\n\n\n\n

\u6211\u5011\u767c\u73fe\u55ae\u7368\u6dfb\u52a0 ZnOAc2 \u4e5f\u6e1b\u5c11\u4e86\u75c5\u6bd2\u8907\u88fd\uff0c\u4f46\u50c5\u5728\u63a5\u8fd1 Vero-E6 \u7d30\u80de\u4e2d ZnOAc2 \u7684 50% \u7d30\u80de\u6bd2\u6027\u6fc3\u5ea6\uff08CC50\uff09\u7684\u6c34\u5e73\uff08~70 \u00b5M\uff0c\u6578\u64da\u672a\u986f\u793a\uff09\u3002\u9019\u53ef\u80fd\u662f\u7531\u65bc Zn2+ \u5728\u542b\u78f7\u9178\u9e7d\u7684\u57f9\u990a\u57fa\u4e2d\u6eb6\u89e3\u5ea6\u5dee\uff0c\u4ee5\u53ca\u5728\u6c92\u6709\u92c5\u96e2\u5b50\u8f09\u9ad4\u7684\u60c5\u6cc1\u4e0b\u7d30\u80de\u5c0d Zn2+ \u7684\u5438\u6536\u6548\u7387\u4f4e\u4e0b\u3002\u3008View Article\u3009<\/p>\n\n\n\n

\u3010\u8a0e\u8ad609\u3011 \u55ae\u7528\u5421\u5576\u786b\u916e(PT)\u8207\u4f75\u7528\u92c5\u96e2\u5b50\u7684\u6548\u679c\u6709\u4f55\u5dee\u7570\u5462\uff1f<\/p>\n\n\n\n

\u901a\u904e\u55ae\u7368\u6dfb\u52a0 PT\uff0cSARS-CoV-GFP \u548c EAV-GFP \u7684\u5831\u544a\u57fa\u56e0\u8868\u9054\u5df2\u7d93\u4ee5\u5291\u91cf\u4f9d\u8cf4\u6027\u65b9\u5f0f\u986f\u8457\u6291\u5236\u3002\u7576\u5411\u57f9\u990a\u57fa\u4e2d\u52a0\u5165 2 \u00b5M Zn2+ \u6642\uff0c\u9019\u7a2e\u6548\u679c\u6703\u986f\u8457\u589e\u5f37\u3002<\/p>\n\n\n\n

\u4f46\u662f2 \u00b5M PT \u548c 2 \u00b5M ZnOAc2 \u7684\u7d44\u5408\u4f7f EAV-GFP \u548c SARS-CoV-GFP \u7684 GFP \u4fe1\u865f\u5206\u5225\u964d\u4f4e\u4e86 98\u00b11% \u548c 85\u00b13%\u3002\u5c0d\u65bc PT\n\u548c ZnOAc2 \u6fc3\u5ea6\u7684\u9019\u7a2e\u7d44\u5408\uff0c\u4e5f\u6c92\u6709\u89c0\u5bdf\u5230\u7d30\u80de\u6bd2\u6027\u3002\u3008View\nArticle\u3009<\/p>\n\n\n\n

\u3010\u8a0e\u8ad610\u3011 \u4f55\u7a2e\u92c5\u96e2\u5b50\u8f09\u9ad4\u53ef\u4ee5\u6291\u5236 RNA \u75c5\u6bd2\u7684\u8907\u5236\u5462\uff1f<\/p>\n\n\n\n

\u5728\u7d30\u80de\u57f9\u990a\u7814\u7a76\u4e2d\uff0c\u6211\u5011\u767c\u73fe\u9ad8 Zn2+ \u6fc3\u5ea6\u548c\u6dfb\u52a0\u523a\u6fc0\u7d30\u80de\u8f38\u5165 Zn2+ \u7684\u5316\u5408\u7269\uff0c\u5982\u6a9c\u9187 (HK)\u3001\u5421\u54af\u70f7\u4e8c\u786b\u4ee3\u6c28\u57fa\u7532\u9178\u916f (PDTC) \u548c\u5421\u5576\u786b\u916e (PT)\uff0c\u53ef\u6291\u5236\u5404\u7a2e RNA \u75c5\u6bd2\u7684\u8907\u5236\uff0c\u5305\u62ec\u6d41\u611f\u75c5\u6bd2\u3001\u547c\u5438\u9053\u5408\u80de\u75c5\u6bd2\u548c\u5e7e\u7a2e\u5c0f\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u3002\u3008View\nArticle\u3009\u3008View Article\u3009\u3008View\nArticle\u3009\u3008View Article\u3009\u3008View\nArticle\u3009\u3008View Article\u3009<\/p>\n\n\n\n

\u5421\u5576\u786b\u916e(PT)\u8207\u92c5\u96e2\u5b50\u7684\u87af\u5408\u7269\u5c31\u7a31\u4f5c\u70ba\u5421\u5576\u786b\u916e\u92c5\u6216\u5967\u9ea5\u4e01\u92c5\uff0c\u7c21\u7a31PTZ\uff0c\u5176\u5316\u5b78\u5f0f\u662fC10H8N2O2S2Zn\uff0c\u5176\u80fd\u4f5c\u70ba\u63a8\u52d5\u92c5\u96e2\u5b50\u7a7f\u900f\u7d30\u80de\u76ae\u819c\u800c\u9032\u5230\u7d30\u80de\u5167\u90e8\u53bb\u6291\u5236\u53ef\u652f\u6301\u75c5\u6bd2RNA\u8907\u88fd\u7684RdRps\u7684\u6d3b\u6027\uff0c\u4f9d\u64da \u91cd\u5fb7 \u7684\u7814\u7a76\u731c\u6e2c\u539f\u56e0\u4e3b\u8981\u662fPTZ\u70ba\u87af\u5408\u9ad4\uff0c\u4e26\u5177\u6709\u6975\u4f4e\u7684\u6eb6\u89e3\u5ea6\uff08\u7d048 ppm\uff09\uff0c\u4e5f\u5f88\u53ef\u80fd\u8207\u5176\u662f\u826f\u597d\u7684\u6bba\u771f\u83cc\u5291\u548c\u6bba\u85fb\u5291\u6709\u95dc\u3002<\/p>\n\n\n\n

\u91cd\u5fb7\u8a8d\u70ba\uff0c\u56e0\u70ba\u5176\u5177\u6709\u751a\u4f4e\u6eb6\u89e3\u5ea6\u4f7f\u5f97PTZ\u53ef\u4ee5\u5b8c\u6574\u7121\u640d\u7684\u5230\u9054\u7d30\u80de\u58c1\uff0c\u66f4\u56e0\u70ba\u5176\u5177\u6709\u6bba\u771f\u83cc\u8207\u6bba\u85fb\u7684\u5929\u6027\uff0c\u800c\u4f7f\u5f97PTZ\u6703\u5faa\u8457\u6a19\u9776\u75c5\u6bd2\u7684\u8ecc\u8de1\u53bb\u6bba\u83cc\u3002\u800c\u4e14PTZ\u5728\u5230\u9054\u88ab\u75c5\u6bd2\u4fb5\u5165\u7684\u7d30\u80de\u58c1\u6642\uff0c\u6703\u56e0\u70ba\u8981\u87af\u5408\u90a3\u4e00\u4e9b\u75c5\u6bd2\u7684\u6b98\u9ad4\u800c\u6703\u91cb\u653e\u51faZn2+\uff0c\u56e0\u6b64Zn2+ \u5c31\u81ea\u7136\u5faa\u8457\u75c5\u6bd2\u8ecc\u8de1\u800c\u7a7f\u900f\u7d30\u80de\u58c1\uff0c\u4e26\u9032\u5165\u7d30\u80de\u4f86\u6291\u5236RdRps\u7684\u6d3b\u6027\u3002<\/p>\n\n\n\n

\u91cd\u5fb7\u7684\u9019\u4e00\u500b\u8ad6\u9ede\u4e5f\u53ef\u4ee5\u7528\u65bc\u8aaa\u660e\u70ba\u4f55\u4f0a\u7dad\u83cc\u7d20\uff08ivermectin\uff09\u4e5f\u6709\u4e0d\u932f\u7684\u9632\u6cbbCOVID-19\u7684\u6548\u679c\uff0c\u56e0\u70ba\u4f0a\u7dad\u83cc\u7d20\u4e4b\u6240\u4ee5\u662f\u4e00\u7a2e\u5f88\u6709\u6548\u7684\u6bba\u87f2\u5291\uff0c\u662f\u56e0\u70ba\u5176\u7528\u6a5f\u5236\u662f\u4f7f\u589e\u52a0\u7121\u810a\u690e\u52d5\u7269\u7684\u7d30\u80de\u58c1\u53ef\u900f\u6027\uff0c\u56e0\u6b64\u5b83\u81ea\u7136\u4e5f\u53ef\u80fd\u6703\u7a7f\u900f\u53d7\u75c5\u6bd2\u611f\u67d3\u7684\u7d30\u80de\u58c1\u800c\u9032\u53bb\u6bba\u6b7b\u75c5\u6bd2\uff0c\u4f46\u662f\u9019\u662f\u4e00\u7a2e\u6bba\u622e\u65b9\u5f0f\uff0c\u5c0dSARS-CoV-2\u7684\u534a\u6291\u5236\u6fc3\u5ea6\u5927\u7d04\u70ba2.2\u20132.8\u03bcM\uff0c\u56e0\u6b64\u82e5\u8981\u5c0dCOVID-19\u7522\u751f\u6291\u5236\u6548\u679c\u5247\u5176\u6240\u9700\u5291\u91cf\u9060\u9ad8\u65bc\u4eba\u7528\u6700\u9ad8\u6279\u51c6\u5291\u91cf\u3001\u4ea6\u8d85\u904e\u53ef\u5b89\u5168\u5be6\u73fe\u5291\u91cf\u3002\u56e0\u6b64\u9060\u4e0d\u5982PTZ\u4f86\u7684\u5b89\u5168\u6709\u6548\u3002<\/p>\n\n\n\n

\u3010\u8a0e\u8ad611\u3011 \u92c5\u96e2\u5b50\u5c0d\u6d41\u611f\u75c5\u6bd2\u8207SARS\u75c5\u6bd2\u7684\u6291\u5236\u4f5c\u7528\u6709\u4f55\u7570\u540c\u5462\uff1f<\/p>\n\n\n\n

\u5be6\u9a57\u8b49\u5be6\uff0c\u4f4e\u6fc3\u5ea6 Zn2+ \u548c PT\uff082 \u00b5M Zn2+ \u548c 2 \u00b5M\nPT\uff09\u7684\u7d44\u5408\u53ef\u6291\u5236\u7d30\u80de\u57f9\u990a\u4e2d SARS \u51a0\u72c0\u75c5\u6bd2\n(SARS-CoV) \u548c\u99ac\u52d5\u8108\u708e\u75c5\u6bd2 (EAV) \u7684\u8907\u5236\u3002\u56e0\u70ba\u9019\u5169\u7a2e\u9060\u89aa\u5de2\u72c0\u75c5\u6bd2\u7684 RNA \u5408\u6210\u7531\u65bc\u4f9d\u8cf4\u65bc\u7d30\u80de\u5167\u539f\u6709\u7684 RNA \u805a\u5408\u9176 (RdRps) \u4f86\u50ac\u5316\uff0c\u56e0\u6b64\uff0cRdRps \u662f\u5176\u591a\u86cb\u767d\u8907\u88fd\u548c\u8f49\u9304\u8907\u5408\u7269 (RTC) \u7684\u6838\u5fc3\u9176\u3002\u800cZn2+ \u88abPT\u87af\u5408\u5f8c\uff0c\u5c07\u5f88\u6709\u6548\u7387\u5730\u7a7f\u900f\u7d30\u80de\u58c1\u800c\u88ab\u9001\u9032\u7d30\u80de\u4e2d\u53bb\u6291\u5236RdRps\uff0c\u56e0\u6b64\u53ea\u8981\u67092 \u00b5M Zn2+ \u4fbf\u8db3\u4ee5\u9577\u671f\u6291\u5236RdRps\u7684\u6d3b\u6027\u4e86\u3002\u3008View Article\u3009<\/p>\n\n\n\n

\u4f46\u662fZn2+ \u96d6\u7136\u6703\u963b\u65b7 EAV RNA \u5408\u6210\u7684\u8d77\u59cb\u6b65\u9a5f\uff0c\u4f46\u662f\u5c0d\u65bc\u75c5\u6bd2\u5ef6\u4f38\u671f\u7684\u6291\u5236\u6548\u679c\u4e26\u6c92\u6709\u90a3\u9ebc\u7406\u60f3\u3002\u3008View\nArticle\u3009<\/p>\n\n\n\n

EAV-GFP \u7de8\u78bc GFP \u8207\u75c5\u6bd2\u975e\u7d50\u69cb\u86cb\u767d 2 (nsp2) \u7684 N \u7aef\u878d\u5408\uff0c\u75c5\u6bd2\u975e\u7d50\u69cb\u86cb\u767d 2 (nsp2) \u662f\u8907\u5236\u9176\u591a\u86cb\u767d\u7684\u5207\u5272\u7522\u7269\u4e4b\u4e00\uff0c\u56e0\u6b64\u70ba\u8907\u5236\u9176\u57fa\u56e0\u7684\u7ffb\u8b6f\u63d0\u4f9b\u76f4\u63a5\u8b80\u6578\u3002\u3008View\nArticle\u3009<\/p>\n\n\n\n

\u9019\u662f\u56e0\u70ba\u5206\u6bb5\u8ca0\u93c8 RNA \u75c5\u6bd2\uff08\u4f8b\u5982\u6d41\u611f\u75c5\u6bd2\uff09\u7684\u8907\u5236\u4e0d\u4f9d\u8cf4\u65bc\u591a\u86cb\u767d\u52a0\u5de5\uff0c\u56e0\u6b64PDTC\u4ecb\u5c0e\u7684Zn2+ \u8f38\u5165\u7684\u5f71\u97ffEAV\nRNA \u5408\u6210\u7684\u8d77\u59cb\u6b65\uff0c\u5176\u5be6\u662f\u7531\u65bc Zn2+ \u6291\u5236\u4e86\u75c5\u6bd2 RNA\u4f9d\u8cf4\u6027RNA\u805a\u5408\u9176(RdRp)\u548c\u7d30\u80de\u8f14\u52a9\u56e0\u5b50\uff0c\u4f46\u662f\u4e00\u65e6\u767c\u5c55\u51fa\u8ca0\u93c8 RNA \u75c5\u6bd2\u5f8c\u4fbf\u518d\u7531\u8ca0\u80a1\u7522\u751f\u51fa\u8a31\u591a\u65b0\u7684RNA\uff08\u6b63\u80a1\uff09\uff0c\u6210\u70ba\u8907\u88fd\u4e2d\u9593\u9ad4\u3002\u6b64\u65b0\u7684\u6b63\u80a1RNA\u53ef\u4f5c\u70bamRNA\u5408\u6210\u8863\u6bbc\u86cb\u767d(\u8acb\u53c3\u8003\u3010\u8a0e\u8ad601\u3011)\u3002\u3008View Article\u3009<\/p>\n\n\n\n

\u7136\u800c\u5728 SARS-CoV \u7684\u60c5\u6cc1\u4e0b\uff0cRdRps \u7684\u5ef6\u4f38\u4e00\u76f4\u53d7\u5230Zn2+ \u6291\u5236\uff0c\u56e0\u6b64\u6a21\u677f\u7684\u7d50\u5408\u6e1b\u5c11\u3002\u3008View Article\u3009<\/p>\n\n\n\n

\u5728 SARS-CoV-GFP \u4e2d\uff0c\u5831\u544a\u57fa\u56e0\u5f9e sg mRNA 7 \u958b\u59cb\u8868\u9054\uff0c\u5728\u66ff\u63db\u4e86\u5169\u500b\u8f14\u52a9\u86cb\u767d\u7de8\u78bc\u57fa\u56e0\uff08ORF 7a \u548c 7b\uff09\u4e4b\u5f8c\uff0c\u9019\u4e9b\u57fa\u56e0\u5c0d\u65bc\u7d30\u80de\u57f9\u990a\u4e2d\u7684\u8907\u88fd\u662f\u53ef\u6709\u53ef\u7121\u7684\u3002\u3008View Article\u3009<\/p>\n\n\n\n

\u3010\u8a0e\u8ad612\u3011 \u70ba\u4f55MgEDTA\u53ef\u4ee5\u9006\u8f49\u92c5\u96e2\u5b50\u7684\u6291\u5236\u75c5\u6bd2\u4f5c\u7528\u5462\uff1f<\/p>\n\n\n\n

MgEDTA (\u4e59\u4e8c\u80fa\u56db\u4e59\u9178\u4e8c\u9209\u9382\uff09\u662f\u4e00\u7a2e\u87af\u5408\u5291\u53ef\u7528\u4ee5\u9006\u8f49 Zn2+ \u4ecb\u5c0e\u7684\u6291\u88fd\u4f5c\u7528\uff0c\u56e0\u70ba\u5b83\u5728\u91cb\u653e Mg2+ \u7684\u540c\u6642\u7279\u7570\u6027\u87af\u5408 Zn2+\uff0c\u9019\u662f\u7531\u65bc ZnEDTA \u8907\u5408\u7269\u5177\u6709\u66f4\u9ad8\u7684\u7a69\u5b9a\u6027\u5e38\u6578\u3002\u3008View Article\u3009<\/p>\n\n\n\n

\u800c\u4e14\uff0cMg2+\u4e0d\u80fd\u7522\u751f\u5982\u540cZn2+\u7684\u6291\u5236\u75c5\u6bd2\u6240\u4f9d\u8cf4\u7684RNA\u805a\u5408\u9176\n(RdRp)\u7684\u6548\u679c\uff0c\u6240\u4ee5MgEDTA\u6240\u5e36\u4f86\u7684\u9ad8\u6fc3\u5ea6Mg2+\uff0c\u5c07\u4f7f\u5f97\u75c5\u6bd2\u6240\u4f9d\u8cf4\u7684RNA\u805a\u5408\u9176 (RdRp)\u7684\u6d3b\u6027\u6062\u5fa9\u3002<\/p>\n\n\n\n

\u91cd\u5fb7\u8a8d\u70ba\u7531\u9019\u4e00\u500b\u53ef\u9006\u7684\u5be6\u9a57\uff0c\u986f\u793a\u51faZn2+\u7684\u6291\u5236\u75c5\u6bd2\u8907\u88fd\u4e3b\u8981\u6191\u85c9\u662f\u9ad8\u6fc3\u5ea6\u7684Zn2+\u8b93\u75c5\u6bd2 RNA \u4f9d\u8cf4\u6027 RNA \u805a\u5408\u9176 (RdRp) \u548c\u7d30\u80de\u8f14\u52a9\u56e0\u5b50\u66ab\u6642\u6027\u7684\u5931\u53bb\u6d3b\u6027\uff0c\u800c\u4e0d\u662f\u6d88\u6ec5\u5b83\uff0c\u56e0\u6b64\u7b97\u662f\u4e00\u7a2e\u8b93\u75c5\u6bd2\u8907\u88fd\u529f\u80fd\u5448\u73fe\u201c\u660f\u8ff7\u72c0\u614b\u201d\u3002<\/p>\n\n\n\n

\u91cd\u5fb7\u8a8d\u70ba\u65e2\u7136\u63d0\u4f9b\u9ad8\u6fc3\u5ea6\u7684Zn2+ \u4e5f\u53ea\u80fd\u8b93\u88ab\u611f\u67d3\u8005\u4e0d\u767c\u75c5\u8207\u4e0d\u5177\u50b3\u67d3\u529b\u800c\u5df2\uff0c\u56e0\u6b64\u5f88\u53ef\u80fd\u9019\u4e00\u4e9b\u75c5\u6bd2\u5c07\u9577\u671f\u6f5b\u4f0f\u5728\u4eba\u9ad4\u4e2d\uff0c\u56e0\u6b64\uff0c\u91cd\u5fb7\u8a8d\u70ba\u6700\u597d\u7684\u9632\u6cbb\u65b9\u6cd5\u5c31\u662f\u4e0d\u8b93\u75c5\u6bd2\u4fb5\u5165\u4eba\u9ad4\uff0c\u4e5f\u5c31\u662f\u5728\u201c\u9ad4\u5916\u201d\u5c31\u56e0\u70ba\u201c\u96fb\u4f4d\u4e2d\u548c\u201d\u800c\u88ab\u6d88\u6ec5\u6389\uff0c\u5176\u6b21\u624d\u662f\u5728\u9ad4\u5167\u9032\u884c\u6709\u6548\u7684\u201c\u6291\u5236\u8907\u88fd\u201d\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

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\u5305\u542b\uff1aAlphatetraviridae\u3001\u8702\u7aa9\u72c0\u75c5\u6bd2\u79d1\uff08Alvernaviridae\uff09\u3001\u661f\u72c0\u75c5\u6bd2\u79d1\uff08Astroviridae\uff09\u3001\u687f\u83cc\u72c0\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u79d1\uff08Barnaviridae\uff09\u3001\u751c\u83dc\u58de\u6b7b\u9ec3\u8108\u75c5\u6bd2\u79d1\uff08Benyviridae\uff09\u3001\u96c0\u9ea5\u82b1\u8449\u75c5\u6bd2\u79d1\uff08Bromoviridae\uff09\u3001\u676f\u72c0\u75c5\u6bd2\u79d1\uff08Caliciviridae\uff09\uff1a\u5d4c\u676f\u75c5\u6bd2\u79d1\uff08Carmotetraviridae\uff09\u3001\u4fee\u9053\u9662\u75c5\u6bd2\u79d1\uff08Closteroviridae\uff09\u3001\u9ec3\u75c5\u6bd2\u79d1\uff08Flaviviridae\uff09\u3001\u809d\u708e\u75c5\u6bd2\u79d1\uff08Hepeviridae\uff09\u3001\u4f4e\u6bd2\u6027\u75c5\u6bd2\u79d1\uff08Hypoviridae\uff09\u3001\u5149\u6ed1\u75c5\u6bd2\u79d1\uff08Leviviridae\uff09\u3001\u9ec3\u75c7\u75c5\u6bd2\u79d1\uff08Luteoviridae\uff09\u3001\u88f8\u9732\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\uff08Narnaviridae)\u3001\u91ce\u7530\u75c5\u6bd2\u79d1\uff08Nodaviridae\uff09\u3001\u6d3e\u6c0f\u75c5\u6bd2\u79d1\uff08Permutotetraviridae\uff09\u3001\u99ac\u9234\u85afY\u75c5\u6bd2\u79d1\uff08Potyviridae\uff09\u3001\u62ab\u8863\u75c5\u6bd2\u79d1\uff08Togaviridae\uff09\u3001\u756a\u8304\u53e2\u77ee\u75c5\u6bd2\u79d1\uff08Tombusviridae\uff09\u3001\u5e1a\u72c0\u75c5\u6bd2\u79d1\uff08Virgaviridae\uff09\u7b49\u3002 \u3010\u8a0e\u8ad603\u3011 \u5728\u6b63\u93c8RNA\u75c5\u6bd2\u7684\u591a\u7a2e\u8907\u5236\u7b56\u7565\u4e2d\u5c45\u95dc\u9375\u56e0\u7d20\u8005\u70ba\u4f55\uff1f \u6b63\u93c8RNA (+RNA) \u75c5\u6bd2\u5305\u62ec\u8a31\u591a\u91cd\u8981\u7684\u75c5\u539f\u9ad4\u3002\u5b83\u5011\u5df2\u7d93\u9032\u5316\u51fa\u591a\u7a2e\u8907\u5236\u7b56\u7565\uff0c\u4f46\u7d71\u4e00\u7684\u4e8b\u5be6\u662f\u4f9d\u8cf4\u65bc\u7d30\u80de\u5167RNA\u5df2\u5b58\u5728\u7684RNA\u805a\u5408\u9176(RdRps)\u4f5c\u70ba\u75c5\u6bd2RNA\u5408\u6210\u6a5f\u5236\u7684\u6838\u5fc3\u9176\u3002\u800c\u4e14RdRp \u901a\u5e38\u5d4c\u5165\u5728\u7531\u75c5\u6bd2 RNA\u3001\u75c5\u6bd2\u548c\u5bbf\u4e3b\u86cb\u767d\u7d44\u88dd\u800c\u6210\u7684\u819c\u76f8\u95dc\u8907\u88fd\u8907\u5408\u7269\u4e2d\u3002\u56e0\u6b64\uff0c\u6709\u6548\u7684\u6291\u5236RdRps \u662f\u6297\u75c5\u6bd2\u7814\u7a76\u7684\u95dc\u9375\u76ee\u6a19\u3002\u3008View Article\u3009\u3002 \u91cd\u5fb7\u8a8d\u70ba\u7531\u65bc\u6b63\u93c8RNA (+RNA) 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Zn2+\u5c0d\u4f86\u81ea\u9f3b\u75c5\u6bd2\u548c\u4e19\u578b\u809d\u708e\u75c5\u6bd2\u7684\u7d14\u5316RdRps\u7684\u6d3b\u6027\u6709\u6291\u88fd\u4f5c\u7528\u3002\u3008View Article\u3009\u3008View Article\u3009 5. \u92c5\u96e2\u5b50\u88ab\u8b49\u660e\u53ef\u4ee5\u6291\u5236\u611f\u67d3\u7d30\u80de\u548c\u7121\u7d30\u80de\u7cfb\u7d71\u4e2d\u51a0\u72c0\u75c5\u6bd2\u8907\u88fd\u9176\u591a\u86cb\u767d\u52a0\u5de5\u904e\u7a0b\u4e2d\u7684\u67d0\u4e9b\u86cb\u767d\u6c34\u89e3\u88c2\u89e3\u3002\u3008View Article\u3009\u3008View Article\u3009 \u3010\u8a0e\u8ad608\u3011 \u5728\u7f3a\u5c11\u5421\u5576\u786b\u916e(PT)\u60c5\u6cc1\u4e0b\u92c5\u96e2\u5b50\u80fd\u6291\u5236\u75c5\u6bd2\u8907\u88fd\u55ce\uff1f \u6211\u5011\u767c\u73fe\u55ae\u7368\u6dfb\u52a0 ZnOAc2 \u4e5f\u6e1b\u5c11\u4e86\u75c5\u6bd2\u8907\u88fd\uff0c\u4f46\u50c5\u5728\u63a5\u8fd1 Vero-E6 \u7d30\u80de\u4e2d ZnOAc2 \u7684 50% \u7d30\u80de\u6bd2\u6027\u6fc3\u5ea6\uff08CC50\uff09\u7684\u6c34\u5e73\uff08~70 \u00b5M\uff0c\u6578\u64da\u672a\u986f\u793a\uff09\u3002\u9019\u53ef\u80fd\u662f\u7531\u65bc Zn2+ \u5728\u542b\u78f7\u9178\u9e7d\u7684\u57f9\u990a\u57fa\u4e2d\u6eb6\u89e3\u5ea6\u5dee\uff0c\u4ee5\u53ca\u5728\u6c92\u6709\u92c5\u96e2\u5b50\u8f09\u9ad4\u7684\u60c5\u6cc1\u4e0b\u7d30\u80de\u5c0d Zn2+ \u7684\u5438\u6536\u6548\u7387\u4f4e\u4e0b\u3002\u3008View Article\u3009 \u3010\u8a0e\u8ad609\u3011 \u55ae\u7528\u5421\u5576\u786b\u916e(PT)\u8207\u4f75\u7528\u92c5\u96e2\u5b50\u7684\u6548\u679c\u6709\u4f55\u5dee\u7570\u5462\uff1f \u901a\u904e\u55ae\u7368\u6dfb\u52a0 PT\uff0cSARS-CoV-GFP \u548c EAV-GFP \u7684\u5831\u544a\u57fa\u56e0\u8868\u9054\u5df2\u7d93\u4ee5\u5291\u91cf\u4f9d\u8cf4\u6027\u65b9\u5f0f\u986f\u8457\u6291\u5236\u3002\u7576\u5411\u57f9\u990a\u57fa\u4e2d\u52a0\u5165 2 \u00b5M Zn2+ \u6642\uff0c\u9019\u7a2e\u6548\u679c\u6703\u986f\u8457\u589e\u5f37\u3002 \u4f46\u662f2 \u00b5M PT \u548c 2 \u00b5M ZnOAc2 \u7684\u7d44\u5408\u4f7f EAV-GFP \u548c SARS-CoV-GFP \u7684 GFP \u4fe1\u865f\u5206\u5225\u964d\u4f4e\u4e86 98\u00b11% \u548c 85\u00b13%\u3002\u5c0d\u65bc PT \u548c ZnOAc2 \u6fc3\u5ea6\u7684\u9019\u7a2e\u7d44\u5408\uff0c\u4e5f\u6c92\u6709\u89c0\u5bdf\u5230\u7d30\u80de\u6bd2\u6027\u3002\u3008View Article\u3009 \u3010\u8a0e\u8ad610\u3011 \u4f55\u7a2e\u92c5\u96e2\u5b50\u8f09\u9ad4\u53ef\u4ee5\u6291\u5236 RNA \u75c5\u6bd2\u7684\u8907\u5236\u5462\uff1f \u5728\u7d30\u80de\u57f9\u990a\u7814\u7a76\u4e2d\uff0c\u6211\u5011\u767c\u73fe\u9ad8 Zn2+ \u6fc3\u5ea6\u548c\u6dfb\u52a0\u523a\u6fc0\u7d30\u80de\u8f38\u5165 Zn2+ \u7684\u5316\u5408\u7269\uff0c\u5982\u6a9c\u9187 (HK)\u3001\u5421\u54af\u70f7\u4e8c\u786b\u4ee3\u6c28\u57fa\u7532\u9178\u916f (PDTC) \u548c\u5421\u5576\u786b\u916e (PT)\uff0c\u53ef\u6291\u5236\u5404\u7a2e RNA \u75c5\u6bd2\u7684\u8907\u5236\uff0c\u5305\u62ec\u6d41\u611f\u75c5\u6bd2\u3001\u547c\u5438\u9053\u5408\u80de\u75c5\u6bd2\u548c\u5e7e\u7a2e\u5c0f\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u3002\u3008View Article\u3009\u3008View Article\u3009\u3008View Article\u3009\u3008View Article\u3009\u3008View Article\u3009\u3008View Article\u3009 \u5421\u5576\u786b\u916e(PT)\u8207\u92c5\u96e2\u5b50\u7684\u87af\u5408\u7269\u5c31\u7a31\u4f5c\u70ba\u5421\u5576\u786b\u916e\u92c5\u6216\u5967\u9ea5\u4e01\u92c5\uff0c\u7c21\u7a31PTZ\uff0c\u5176\u5316\u5b78\u5f0f\u662fC10H8N2O2S2Zn\uff0c\u5176\u80fd\u4f5c\u70ba\u63a8\u52d5\u92c5\u96e2\u5b50\u7a7f\u900f\u7d30\u80de\u76ae\u819c\u800c\u9032\u5230\u7d30\u80de\u5167\u90e8\u53bb\u6291\u5236\u53ef\u652f\u6301\u75c5\u6bd2RNA\u8907\u88fd\u7684RdRps\u7684\u6d3b\u6027\uff0c\u4f9d\u64da \u91cd\u5fb7 \u7684\u7814\u7a76\u731c\u6e2c\u539f\u56e0\u4e3b\u8981\u662fPTZ\u70ba\u87af\u5408\u9ad4\uff0c\u4e26\u5177\u6709\u6975\u4f4e\u7684\u6eb6\u89e3\u5ea6\uff08\u7d048 ppm\uff09\uff0c\u4e5f\u5f88\u53ef\u80fd\u8207\u5176\u662f\u826f\u597d\u7684\u6bba\u771f\u83cc\u5291\u548c\u6bba\u85fb\u5291\u6709\u95dc\u3002 \u91cd\u5fb7\u8a8d\u70ba\uff0c\u56e0\u70ba\u5176\u5177\u6709\u751a\u4f4e\u6eb6\u89e3\u5ea6\u4f7f\u5f97PTZ\u53ef\u4ee5\u5b8c\u6574\u7121\u640d\u7684\u5230\u9054\u7d30\u80de\u58c1\uff0c\u66f4\u56e0\u70ba\u5176\u5177\u6709\u6bba\u771f\u83cc\u8207\u6bba\u85fb\u7684\u5929\u6027\uff0c\u800c\u4f7f\u5f97PTZ\u6703\u5faa\u8457\u6a19\u9776\u75c5\u6bd2\u7684\u8ecc\u8de1\u53bb\u6bba\u83cc\u3002\u800c\u4e14PTZ\u5728\u5230\u9054\u88ab\u75c5\u6bd2\u4fb5\u5165\u7684\u7d30\u80de\u58c1\u6642\uff0c\u6703\u56e0\u70ba\u8981\u87af\u5408\u90a3\u4e00\u4e9b\u75c5\u6bd2\u7684\u6b98\u9ad4\u800c\u6703\u91cb\u653e\u51faZn2+\uff0c\u56e0\u6b64Zn2+ \u5c31\u81ea\u7136\u5faa\u8457\u75c5\u6bd2\u8ecc\u8de1\u800c\u7a7f\u900f\u7d30\u80de\u58c1\uff0c\u4e26\u9032\u5165\u7d30\u80de\u4f86\u6291\u5236RdRps\u7684\u6d3b\u6027\u3002 \u91cd\u5fb7\u7684\u9019\u4e00\u500b\u8ad6\u9ede\u4e5f\u53ef\u4ee5\u7528\u65bc\u8aaa\u660e\u70ba\u4f55\u4f0a\u7dad\u83cc\u7d20\uff08ivermectin\uff09\u4e5f\u6709\u4e0d\u932f\u7684\u9632\u6cbbCOVID-19\u7684\u6548\u679c\uff0c\u56e0\u70ba\u4f0a\u7dad\u83cc\u7d20\u4e4b\u6240\u4ee5\u662f\u4e00\u7a2e\u5f88\u6709\u6548\u7684\u6bba\u87f2\u5291\uff0c\u662f\u56e0\u70ba\u5176\u7528\u6a5f\u5236\u662f\u4f7f\u589e\u52a0\u7121\u810a\u690e\u52d5\u7269\u7684\u7d30\u80de\u58c1\u53ef\u900f\u6027\uff0c\u56e0\u6b64\u5b83\u81ea\u7136\u4e5f\u53ef\u80fd\u6703\u7a7f\u900f\u53d7\u75c5\u6bd2\u611f\u67d3\u7684\u7d30\u80de\u58c1\u800c\u9032\u53bb\u6bba\u6b7b\u75c5\u6bd2\uff0c\u4f46\u662f\u9019\u662f\u4e00\u7a2e\u6bba\u622e\u65b9\u5f0f\uff0c\u5c0dSARS-CoV-2\u7684\u534a\u6291\u5236\u6fc3\u5ea6\u5927\u7d04\u70ba2.2\u20132.8\u03bcM\uff0c\u56e0\u6b64\u82e5\u8981\u5c0dCOVID-19\u7522\u751f\u6291\u5236\u6548\u679c\u5247\u5176\u6240\u9700\u5291\u91cf\u9060\u9ad8\u65bc\u4eba\u7528\u6700\u9ad8\u6279\u51c6\u5291\u91cf\u3001\u4ea6\u8d85\u904e\u53ef\u5b89\u5168\u5be6\u73fe\u5291\u91cf\u3002\u56e0\u6b64\u9060\u4e0d\u5982PTZ\u4f86\u7684\u5b89\u5168\u6709\u6548\u3002 \u3010\u8a0e\u8ad611\u3011 \u92c5\u96e2\u5b50\u5c0d\u6d41\u611f\u75c5\u6bd2\u8207SARS\u75c5\u6bd2\u7684\u6291\u5236\u4f5c\u7528\u6709\u4f55\u7570\u540c\u5462\uff1f \u5be6\u9a57\u8b49\u5be6\uff0c\u4f4e\u6fc3\u5ea6 Zn2+ \u548c PT\uff082 \u00b5M Zn2+ \u548c 2 \u00b5M PT\uff09\u7684\u7d44\u5408\u53ef\u6291\u5236\u7d30\u80de\u57f9\u990a\u4e2d SARS \u51a0\u72c0\u75c5\u6bd2 (SARS-CoV) \u548c\u99ac\u52d5\u8108\u708e\u75c5\u6bd2 (EAV) \u7684\u8907\u5236\u3002\u56e0\u70ba\u9019\u5169\u7a2e\u9060\u89aa\u5de2\u72c0\u75c5\u6bd2\u7684 RNA \u5408\u6210\u7531\u65bc\u4f9d\u8cf4\u65bc\u7d30\u80de\u5167\u539f\u6709\u7684 RNA \u805a\u5408\u9176 (RdRps) \u4f86\u50ac\u5316\uff0c\u56e0\u6b64\uff0cRdRps \u662f\u5176\u591a\u86cb\u767d\u8907\u88fd\u548c\u8f49\u9304\u8907\u5408\u7269 (RTC) \u7684\u6838\u5fc3\u9176\u3002\u800cZn2+ \u88abPT\u87af\u5408\u5f8c\uff0c\u5c07\u5f88\u6709\u6548\u7387\u5730\u7a7f\u900f\u7d30\u80de\u58c1\u800c\u88ab\u9001\u9032\u7d30\u80de\u4e2d\u53bb\u6291\u5236RdRps\uff0c\u56e0\u6b64\u53ea\u8981\u67092 \u00b5M Zn2+ \u4fbf\u8db3\u4ee5\u9577\u671f\u6291\u5236RdRps\u7684\u6d3b\u6027\u4e86\u3002\u3008View Article\u3009 \u4f46\u662fZn2+ \u96d6\u7136\u6703\u963b\u65b7 EAV RNA \u5408\u6210\u7684\u8d77\u59cb\u6b65\u9a5f\uff0c\u4f46\u662f\u5c0d\u65bc\u75c5\u6bd2\u5ef6\u4f38\u671f\u7684\u6291\u5236\u6548\u679c\u4e26\u6c92\u6709\u90a3\u9ebc\u7406\u60f3\u3002\u3008View Article\u3009 EAV-GFP \u7de8\u78bc GFP \u8207\u75c5\u6bd2\u975e\u7d50\u69cb\u86cb\u767d 2 (nsp2) \u7684 N \u7aef\u878d\u5408\uff0c\u75c5\u6bd2\u975e\u7d50\u69cb\u86cb\u767d 2 (nsp2) \u662f\u8907\u5236\u9176\u591a\u86cb\u767d\u7684\u5207\u5272\u7522\u7269\u4e4b\u4e00\uff0c\u56e0\u6b64\u70ba\u8907\u5236\u9176\u57fa\u56e0\u7684\u7ffb\u8b6f\u63d0\u4f9b\u76f4\u63a5\u8b80\u6578\u3002\u3008View Article\u3009 \u9019\u662f\u56e0\u70ba\u5206\u6bb5\u8ca0\u93c8 RNA \u75c5\u6bd2\uff08\u4f8b\u5982\u6d41\u611f\u75c5\u6bd2\uff09\u7684\u8907\u5236\u4e0d\u4f9d\u8cf4\u65bc\u591a\u86cb\u767d\u52a0\u5de5\uff0c\u56e0\u6b64PDTC\u4ecb\u5c0e\u7684Zn2+ \u8f38\u5165\u7684\u5f71\u97ffEAV RNA \u5408\u6210\u7684\u8d77\u59cb\u6b65\uff0c\u5176\u5be6\u662f\u7531\u65bc Zn2+ \u6291\u5236\u4e86\u75c5\u6bd2 RNA\u4f9d\u8cf4\u6027RNA\u805a\u5408\u9176(RdRp)\u548c\u7d30\u80de\u8f14\u52a9\u56e0\u5b50\uff0c\u4f46\u662f\u4e00\u65e6\u767c\u5c55\u51fa\u8ca0\u93c8 RNA \u75c5\u6bd2\u5f8c\u4fbf\u518d\u7531\u8ca0\u80a1\u7522\u751f\u51fa\u8a31\u591a\u65b0\u7684RNA\uff08\u6b63\u80a1\uff09\uff0c\u6210\u70ba\u8907\u88fd\u4e2d\u9593\u9ad4\u3002\u6b64\u65b0\u7684\u6b63\u80a1RNA\u53ef\u4f5c\u70bamRNA\u5408\u6210\u8863\u6bbc\u86cb\u767d(\u8acb\u53c3\u8003\u3010\u8a0e\u8ad601\u3011)\u3002\u3008View Article\u3009 \u7136\u800c\u5728 SARS-CoV \u7684\u60c5\u6cc1\u4e0b\uff0cRdRps \u7684\u5ef6\u4f38\u4e00\u76f4\u53d7\u5230Zn2+ \u6291\u5236\uff0c\u56e0\u6b64\u6a21\u677f\u7684\u7d50\u5408\u6e1b\u5c11\u3002\u3008View Article\u3009 \u5728 SARS-CoV-GFP \u4e2d\uff0c\u5831\u544a\u57fa\u56e0\u5f9e sg mRNA 7 \u958b\u59cb\u8868\u9054\uff0c\u5728\u66ff\u63db\u4e86\u5169\u500b\u8f14\u52a9\u86cb\u767d\u7de8\u78bc\u57fa\u56e0\uff08ORF 7a \u548c 7b\uff09\u4e4b\u5f8c\uff0c\u9019\u4e9b\u57fa\u56e0\u5c0d\u65bc\u7d30\u80de\u57f9\u990a\u4e2d\u7684\u8907\u88fd\u662f\u53ef\u6709\u53ef\u7121\u7684\u3002\u3008View Article\u3009 \u3010\u8a0e\u8ad612\u3011 \u70ba\u4f55MgEDTA\u53ef\u4ee5\u9006\u8f49\u92c5\u96e2\u5b50\u7684\u6291\u5236\u75c5\u6bd2\u4f5c\u7528\u5462\uff1f MgEDTA (\u4e59\u4e8c\u80fa\u56db\u4e59\u9178\u4e8c\u9209\u9382\uff09\u662f\u4e00\u7a2e\u87af\u5408\u5291\u53ef\u7528\u4ee5\u9006\u8f49 Zn2+ \u4ecb\u5c0e\u7684\u6291\u88fd\u4f5c\u7528\uff0c\u56e0\u70ba\u5b83\u5728\u91cb\u653e Mg2+ \u7684\u540c\u6642\u7279\u7570\u6027\u87af\u5408 Zn2+\uff0c\u9019\u662f\u7531\u65bc ZnEDTA \u8907\u5408\u7269\u5177\u6709\u66f4\u9ad8\u7684\u7a69\u5b9a\u6027\u5e38\u6578\u3002\u3008View Article\u3009 \u800c\u4e14\uff0cMg2+\u4e0d\u80fd\u7522\u751f\u5982\u540cZn2+\u7684\u6291\u5236\u75c5\u6bd2\u6240\u4f9d\u8cf4\u7684RNA\u805a\u5408\u9176 (RdRp)\u7684\u6548\u679c\uff0c\u6240\u4ee5MgEDTA\u6240\u5e36\u4f86\u7684\u9ad8\u6fc3\u5ea6Mg2+\uff0c\u5c07\u4f7f\u5f97\u75c5\u6bd2\u6240\u4f9d\u8cf4\u7684RNA\u805a\u5408\u9176 (RdRp)\u7684\u6d3b\u6027\u6062\u5fa9\u3002 \u91cd\u5fb7\u8a8d\u70ba\u7531\u9019\u4e00\u500b\u53ef\u9006\u7684\u5be6\u9a57\uff0c\u986f\u793a\u51faZn2+\u7684\u6291\u5236\u75c5\u6bd2\u8907\u88fd\u4e3b\u8981\u6191\u85c9\u662f\u9ad8\u6fc3\u5ea6\u7684Zn2+\u8b93\u75c5\u6bd2 RNA \u4f9d\u8cf4\u6027 RNA \u805a\u5408\u9176 (RdRp) \u548c\u7d30\u80de\u8f14\u52a9\u56e0\u5b50\u66ab\u6642\u6027\u7684\u5931\u53bb\u6d3b\u6027\uff0c\u800c\u4e0d\u662f\u6d88\u6ec5\u5b83\uff0c\u56e0\u6b64\u7b97\u662f\u4e00\u7a2e\u8b93\u75c5\u6bd2\u8907\u88fd\u529f\u80fd\u5448\u73fe\u201c\u660f\u8ff7\u72c0\u614b\u201d\u3002 \u91cd\u5fb7\u8a8d\u70ba\u65e2\u7136\u63d0\u4f9b\u9ad8\u6fc3\u5ea6\u7684Zn2+ \u4e5f\u53ea\u80fd\u8b93\u88ab\u611f\u67d3\u8005\u4e0d\u767c\u75c5\u8207\u4e0d\u5177\u50b3\u67d3\u529b\u800c\u5df2\uff0c\u56e0\u6b64\u5f88\u53ef\u80fd\u9019\u4e00\u4e9b\u75c5\u6bd2\u5c07\u9577\u671f\u6f5b\u4f0f\u5728\u4eba\u9ad4\u4e2d\uff0c\u56e0\u6b64\uff0c\u91cd\u5fb7\u8a8d\u70ba\u6700\u597d\u7684\u9632\u6cbb\u65b9\u6cd5\u5c31\u662f\u4e0d\u8b93\u75c5\u6bd2\u4fb5\u5165\u4eba\u9ad4\uff0c\u4e5f\u5c31\u662f\u5728\u201c\u9ad4\u5916\u201d\u5c31\u56e0\u70ba\u201c\u96fb\u4f4d\u4e2d\u548c\u201d\u800c\u88ab\u6d88\u6ec5\u6389\uff0c\u5176\u6b21\u624d\u662f\u5728\u9ad4\u5167\u9032\u884c\u6709\u6548\u7684\u201c\u6291\u5236\u8907\u88fd\u201d\u3002\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/pingtungtimes.com.tw\/?p=84674\" \/>\n<meta property=\"og:locale\" content=\"zh_TW\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"12\u500b\u7814\u767c\u6297\u6bd2\u85e5\u7269\u5fc3\u5f97\u8207\u8af8\u53cb\u5206\u4eab - \u5c4f\u6771\u6642\u5831\u96fb\u5b50\u5831\" \/>\n<meta property=\"og:description\" content=\"\u3010\u674e\u91cd\u5fb7\uff0f\u5c08\u6b04\u5831\u5c0e2021\/08\/27\u3011\u3000\u91cd\u5fb7\u7814\u8b80\u4e86\u5e7e\u7bc7\u6709\u95dc\u92c5\u96e2\u5b50\u6291\u5236\u51a0\u72c0\u75c5\u6bd2\u7684\u8ad6\u6587\u4e4b\u5f8c\uff0c\u5df2\u7d93\u7565\u6709\u5fc3\u5f97\u4e86\uff0c\u7576\u7136\uff0c\u9019\u4e9b\u8cc7\u6599\u5c0d\u65bc\u4e00\u500b\u975e\u6b64\u9818\u57df\u7684\u4eba\u4f86\u8aaa\u90fd\u662f\u975e\u5e38\u5de8\u91cf\u800c\u4e14\u7d22\u7136\u7121\u5473\u7684\uff0c\u4f46\u662f\u91cd\u5fb7\u60f3\u8981\u63d0\u7db1\u6308\u9818\u7684\u628a\u5e7e\u500b\u95dc\u9375\u6027\u7684\u91cd\u9ede\u63d0\u51fa\u4f86\u548c\u5404\u4f4d\u597d\u53cb\u8a0e\u8ad6\u8207\u5206\u4eab\uff0c\u56e0\u70ba\u9019\u662f\u5927\u5bb6\u4ea4\u4e86 \u91cd\u5fb7 \u9019\u6a23\u4e00\u500b\u52c7\u65bc\u627f\u64d4\u5927\u4efb\u8005\u7684\u4e00\u500b\u201c\u9810\u77e5\u798f\u5229\u201d\u3002 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(1)\u6b63\u80a1RNA\u75c5\u6bd2\uff08\u5982\u810a\u9ad3\u7070\u8cea\u708e\u75c5\u6bd2\uff09\u7684\u55ae\u80a1RNA\u53ef\u76f4\u63a5\u8d77mRNA\u4f5c\u7528\uff0c\u8f49\u8b6f\u65e9\u671f\u86cb\u767d\u8cea\uff0c\u5305\u62ecRNA\u591a\u805a\u9176\u548c\u6291\u5236\u5bbf\u4e3b\u7d30\u80de\u5408\u6210\u4ee3\u8b1d\u7684\u8abf\u63a7\u86cb\u767d\u3002\u7136\u5f8c\u5728RNA\u591a\u805a\u9176\u7684\u4f5c\u7528\u4e0b\uff0c\u4ee5\u55ae\u80a1RNA\uff08\u6b63\u80a1\uff09\u70ba\u6a21\u677f\uff0c\u5f62\u6210\u4e92\u88dc\u80a1\uff08\u8ca0\u80a1\uff09\uff0c\u5169\u8005\u7d50\u5408\u6210\u96d9\u80a1\uff0c\u7a31\u70ba\u8907\u88fd\u578b\u3002\u518d\u7531\u8ca0\u80a1\u7522\u751f\u51fa\u8a31\u591a\u65b0\u7684RNA\uff08\u6b63\u80a1\uff09\uff0c\u6210\u70ba\u8907\u88fd\u4e2d\u9593\u9ad4\u3002\u6b64\u65b0\u7684\u6b63\u80a1RNA\u53ef\u4f5c\u70bamRNA\u5408\u6210\u8863\u6bbc\u86cb\u767d\u3002 (2)\u8ca0\u80a1RNA\u75c5\u6bd2\uff08\u5982\u6b63\u7c98\u75c5\u6bd2\u3001\u526f\u7c98\u75c5\u6bd2\u3001\u5f48\u72c0\u75c5\u6bd2\uff09\u7684\u55ae\u80a1RNA\u4e0d\u80fd\u4f5c\u70bamRNA\uff0c\u7a31\u70ba\u8ca0\u80a1\uff0c\u9808\u5148\u5408\u6210\u4e92\u88dc\u80a1\uff08\u6b63\u80a1\uff09\u4f5c\u70bamRNA\uff0c\u518d\u8f49\u8b6f\u86cb\u767d\u5206\u5b50\uff0c\u800c\u5f8c\u7522\u751f\u6838\u9178\u7684\u8907\u88fd\u578b\uff0c\u6210\u70ba\u5408\u6210\u5b50\u4ee3\u75c5\u6bd2RNA\u7684\u6a21\u677f\u3002 \u56e0\u6b64\uff0c\u6b63\u93c8RNA\u75c5\u6bd2\u7684\u57fa\u56e0\u7d44\u70ba\u4e00\u689d\u6b63\u80a1\u7684\u55ae\u93c8RNA\uff0c\u75c5\u6bd2\u9ad4\u672c\u8eab\u4e0d\u651c\u5e36RNA\u8907\u88fd\u9176\u3002 \u5176\u907a\u50b3\u4fe1\u606f\u50b3\u905e\u904e\u7a0b\u5206\u6210\u4ee5\u4e0b\u5e7e\u7a2e\u65b9\u5f0f\uff1a (1).\u6b63\u93c8RNA\u9032\u5165\u5bbf\u4e3b\u7d30\u80de\u5f8c\uff0c\u4e00\u4e9b\u76f4\u63a5\u4f5c\u70bamRNA\u93c8\u6307\u5c0e\u86cb\u767d\u8cea\u7684\u5408\u6210\uff1b\u9019\u4e9b\u86cb\u767d\u8cea\u7522\u7269\u6709\u4f9d\u8cf4RNA\u7684RNA\u805a\u5408\u9176(RdRp)\u53ca\u8863\u6bbc\u86cb\u767d\u3002 (2).\u53e6\u4e00\u4e9b\u6b63\u93c8RNA\u53ef\u4ee5\u901a\u904e\u6b64RdRp\u9176\u7684\u4f5c\u7528\uff0c\u751f\u6210\u8ca0\u93c8\uff0c\u5f62\u6210\u96d9\u93c8\u5f62\u5f0f\u7684\u8907\u88fd\u578b\u4e2d\u9593\u9ad4\uff1b\u63a5\u8457\u6b64\u96d9\u93c8\u53ef\u4ee5\u89e3\u93c8\uff0c\u518d\u4ee5\u8ca0\u93c8\u70ba\u6a21\u677f\uff0c\u5728RdRp\u4f5c\u7528\u4e0b\uff0c\u751f\u6210\u5927\u91cf\u65b0\u7684\u6b63\u93c8RNA\uff0c\u9054\u5230\u8907\u88fd\u7684\u76ee\u7684\u3002 (3).\u751f\u6210\u7684\u6b63\u93c8\u53ef\u4f5c\u70bamRNA\uff0c\u6307\u5c0e\u86cb\u767d\u8cea\u7684\u5408\u6210\u6216\u4f5c\u70ba\u75c5\u6bd2\u57fa\u56e0\u7d44\u3002 (4).\u8863\u6bbc\u86cb\u767d\u5305\u88f9\u6b63\u93c8RNA\uff0c\u7d44\u88dd\u5f62\u6210\u5b50\u4ee3\u75c5\u6bd2\u3002 \u3010\u8a0e\u8ad602\u3011 \u6b63\u93c8RNA\u75c5\u6bd2\u5305\u542b\u54ea\u4e00\u4e9b\u75c5\u6bd2\u7a2e\u985e\u5462\uff1f (1). \u7db2\u5de2\u75c5\u6bd2\u76ee\uff08Nidovirales\uff09 \u5305\u542b\uff1b\u52d5\u8108\u708e\u75c5\u6bd2\u79d1\uff08Arteriviridae\uff09\u3001\u51a0\u72c0\u75c5\u6bd2\u79d1\uff08Coronaviridae\uff09\u3001\u6d77\u6d0b\u75c5\u6bd2\u79d1\uff08Mesoniviridae\uff09\u3001\u687f\u72c0\u5957\u75c5\u6bd2\u79d1\uff08Roniviridae\uff09\u3002 (2). \u5c0f\u6838\u7cd6\u6838\u9178\u76ee\uff08Picornavirales\uff09 \u5305\u542b\uff1a\u4e8c\u9806\u53cd\u5b50\u75c5\u6bd2\u79d1\uff08Dicistroviridae\uff09\u3001\u50b3\u67d3\u6027\u8edf\u5316\u75c5\u6bd2\u79d1\uff08Iflaviridae\uff09\u3001\u6d77\u6d0bRNA\u75c5\u6bd2\u79d1\uff08Marnaviridae\uff09\u3001\u5fae\u5c0f\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u79d1\uff08Picornaviridae\uff09\u3001\u4f34\u751f\u8c47\u8c46\u75c5\u6bd2\u79d1(Secoviridae)\u3001\u8c47\u8c46\u82b1\u8449\u75c5\u6bd2\u4e9e\u79d1\uff08Comoviridae\uff09\u3001Bacillarnavirus\u3001Labyrnavirus\u3002 (3). \u856a\u83c1\u9ec3\u82b1\u8449\u75c5\u6bd2\u76ee(Tymovirales) \u5305\u542b\uff1a\u7532\u578b\u7dda\u5f62\u75c5\u6bd2\u79d1\uff08Alphaflexiviridae\uff09\u3001\u4e59\u578b\u7dda\u5f62\u75c5\u6bd2\u79d1(Betaflexiviridae)\u3001\u4e19\u578b\u7dda\u5f62\u75c5\u6bd2\u79d1(Gammaflexiviridae)\u3001\u856a\u83c1\u767c\u9ec3\u9472\u5d4c\u75c5\u6bd2\u79d1\uff08Tymoviridae\uff09\u3002 (4). \u672a\u8a2d\u76ee\u7684\u75c5\u6bd2\u79d1 \u5305\u542b\uff1aAlphatetraviridae\u3001\u8702\u7aa9\u72c0\u75c5\u6bd2\u79d1\uff08Alvernaviridae\uff09\u3001\u661f\u72c0\u75c5\u6bd2\u79d1\uff08Astroviridae\uff09\u3001\u687f\u83cc\u72c0\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u79d1\uff08Barnaviridae\uff09\u3001\u751c\u83dc\u58de\u6b7b\u9ec3\u8108\u75c5\u6bd2\u79d1\uff08Benyviridae\uff09\u3001\u96c0\u9ea5\u82b1\u8449\u75c5\u6bd2\u79d1\uff08Bromoviridae\uff09\u3001\u676f\u72c0\u75c5\u6bd2\u79d1\uff08Caliciviridae\uff09\uff1a\u5d4c\u676f\u75c5\u6bd2\u79d1\uff08Carmotetraviridae\uff09\u3001\u4fee\u9053\u9662\u75c5\u6bd2\u79d1\uff08Closteroviridae\uff09\u3001\u9ec3\u75c5\u6bd2\u79d1\uff08Flaviviridae\uff09\u3001\u809d\u708e\u75c5\u6bd2\u79d1\uff08Hepeviridae\uff09\u3001\u4f4e\u6bd2\u6027\u75c5\u6bd2\u79d1\uff08Hypoviridae\uff09\u3001\u5149\u6ed1\u75c5\u6bd2\u79d1\uff08Leviviridae\uff09\u3001\u9ec3\u75c7\u75c5\u6bd2\u79d1\uff08Luteoviridae\uff09\u3001\u88f8\u9732\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\uff08Narnaviridae)\u3001\u91ce\u7530\u75c5\u6bd2\u79d1\uff08Nodaviridae\uff09\u3001\u6d3e\u6c0f\u75c5\u6bd2\u79d1\uff08Permutotetraviridae\uff09\u3001\u99ac\u9234\u85afY\u75c5\u6bd2\u79d1\uff08Potyviridae\uff09\u3001\u62ab\u8863\u75c5\u6bd2\u79d1\uff08Togaviridae\uff09\u3001\u756a\u8304\u53e2\u77ee\u75c5\u6bd2\u79d1\uff08Tombusviridae\uff09\u3001\u5e1a\u72c0\u75c5\u6bd2\u79d1\uff08Virgaviridae\uff09\u7b49\u3002 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\u6211\u5011\u6e2c\u8a66\u4e86 Zn2+ \u6dfb\u52a0\u5c0d RTC \u6d3b\u6027\u7684\u5f71\u97ff\u3002\u5c0d\u65bc EAV\u548c SARS-CoV\uff0c\u7576\u5b58\u5728 ZnOAc2 \u6642\uff0c\u89c0\u5bdf\u5230\u5408\u6210\u7684 RNA \u91cf\u5448\u5291\u91cf\u4f9d\u8cf4\u6027\u4e0b\u964d\u3002\u5c0d\u65bc\u9019\u5169\u7a2e\u75c5\u6bd2\uff0c\u5728 50 \u00b5M \u7684 Zn2+ \u6fc3\u5ea6\u4e0b\u89c0\u5bdf\u5230\u7e3d RNA \u5408\u6210\u6e1b\u5c11\u4e86 50% \u4ee5\u4e0a\uff0c\u800c\u5728 500 \u00b5M \u7684 Zn2+ \u6fc3\u5ea6\u4e0b\u4ecd\u4fdd\u6301\u4e0d\u5230 5% \u7684\u6d3b\u6027\u3002\u57fa\u56e0\u7d44\u5408\u6210\u548c sg mRNA \u7522\u751f\u540c\u6a23\u53d7\u5230\u5f71\u97ff\u3002\u3008View Article\u3009 \u3010\u8a0e\u8ad607\u3011 \u9069\u7576\u6fc3\u5ea6\u92c5\u96e2\u5b50\u5c0d\u54ea\u4e9b\u75c5\u6bd2\u7684\u8907\u88fd\u6709\u6291\u5236\u6548\u679c\u5462\uff1f 1. \u91dd\u5c0d\u5c0f\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u9032\u884c\u4e86\u5408\u7406\u8a73\u7d30\u7684\u7814\u7a76\u5f8c\u8b49\u660e\u5728\u7d30\u80de\u57f9\u990a\u4e2d\uff0cPT \u5728\u6578\u5206\u9418\u5167\u523a\u6fc0Zn2+\u651d\u53d6\u4e26\u901a\u904e\u4e00\u7a2e\u6a5f\u5236\u6291\u5236 RNA \u75c5\u6bd2\u8907\u88fd\u3002\u3008View Article\u3009\u3008View Article\u3009 2. \u5c0d\u7d14\u5316\u7684\u9f3b\u75c5\u6bd2\u548c\u810a\u9ad3\u7070\u8cea\u708e\u75c5\u6bd2 3C \u86cb\u767d\u9176\u9032\u884c\u7684\u9ad4\u5916\u7814\u7a76\u8868\u660e\uff0c\u86cb\u767d\u9176\u6d3b\u6027\u88ab Zn2+ \u6291\u5236\u3002\u3008View Article\u3009\u3008View Article\u3009 3. \u5728\u611f\u67d3\u4eba\u9f3b\u75c5\u6bd2\u548c\u67ef\u85a9\u5947\u75c5\u6bd2B3\u7684\u7d30\u80de\u4e2d\u89c0\u5bdf\u5230\u7684\u92c5\u96e2\u5b50\u5c0d\u591a\u86cb\u767d\u52a0\u5de5\u7684\u6291\u88fd\u4f5c\u7528\u3002\u3008View Article\u3009\u3008View Article\u3009 4. Zn2+\u5c0d\u4f86\u81ea\u9f3b\u75c5\u6bd2\u548c\u4e19\u578b\u809d\u708e\u75c5\u6bd2\u7684\u7d14\u5316RdRps\u7684\u6d3b\u6027\u6709\u6291\u88fd\u4f5c\u7528\u3002\u3008View Article\u3009\u3008View Article\u3009 5. \u92c5\u96e2\u5b50\u88ab\u8b49\u660e\u53ef\u4ee5\u6291\u5236\u611f\u67d3\u7d30\u80de\u548c\u7121\u7d30\u80de\u7cfb\u7d71\u4e2d\u51a0\u72c0\u75c5\u6bd2\u8907\u88fd\u9176\u591a\u86cb\u767d\u52a0\u5de5\u904e\u7a0b\u4e2d\u7684\u67d0\u4e9b\u86cb\u767d\u6c34\u89e3\u88c2\u89e3\u3002\u3008View Article\u3009\u3008View Article\u3009 \u3010\u8a0e\u8ad608\u3011 \u5728\u7f3a\u5c11\u5421\u5576\u786b\u916e(PT)\u60c5\u6cc1\u4e0b\u92c5\u96e2\u5b50\u80fd\u6291\u5236\u75c5\u6bd2\u8907\u88fd\u55ce\uff1f \u6211\u5011\u767c\u73fe\u55ae\u7368\u6dfb\u52a0 ZnOAc2 \u4e5f\u6e1b\u5c11\u4e86\u75c5\u6bd2\u8907\u88fd\uff0c\u4f46\u50c5\u5728\u63a5\u8fd1 Vero-E6 \u7d30\u80de\u4e2d ZnOAc2 \u7684 50% \u7d30\u80de\u6bd2\u6027\u6fc3\u5ea6\uff08CC50\uff09\u7684\u6c34\u5e73\uff08~70 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\u4fe1\u865f\u5206\u5225\u964d\u4f4e\u4e86 98\u00b11% \u548c 85\u00b13%\u3002\u5c0d\u65bc PT \u548c ZnOAc2 \u6fc3\u5ea6\u7684\u9019\u7a2e\u7d44\u5408\uff0c\u4e5f\u6c92\u6709\u89c0\u5bdf\u5230\u7d30\u80de\u6bd2\u6027\u3002\u3008View Article\u3009 \u3010\u8a0e\u8ad610\u3011 \u4f55\u7a2e\u92c5\u96e2\u5b50\u8f09\u9ad4\u53ef\u4ee5\u6291\u5236 RNA \u75c5\u6bd2\u7684\u8907\u5236\u5462\uff1f \u5728\u7d30\u80de\u57f9\u990a\u7814\u7a76\u4e2d\uff0c\u6211\u5011\u767c\u73fe\u9ad8 Zn2+ \u6fc3\u5ea6\u548c\u6dfb\u52a0\u523a\u6fc0\u7d30\u80de\u8f38\u5165 Zn2+ \u7684\u5316\u5408\u7269\uff0c\u5982\u6a9c\u9187 (HK)\u3001\u5421\u54af\u70f7\u4e8c\u786b\u4ee3\u6c28\u57fa\u7532\u9178\u916f (PDTC) \u548c\u5421\u5576\u786b\u916e (PT)\uff0c\u53ef\u6291\u5236\u5404\u7a2e RNA \u75c5\u6bd2\u7684\u8907\u5236\uff0c\u5305\u62ec\u6d41\u611f\u75c5\u6bd2\u3001\u547c\u5438\u9053\u5408\u80de\u75c5\u6bd2\u548c\u5e7e\u7a2e\u5c0f\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u3002\u3008View Article\u3009\u3008View Article\u3009\u3008View Article\u3009\u3008View Article\u3009\u3008View Article\u3009\u3008View Article\u3009 \u5421\u5576\u786b\u916e(PT)\u8207\u92c5\u96e2\u5b50\u7684\u87af\u5408\u7269\u5c31\u7a31\u4f5c\u70ba\u5421\u5576\u786b\u916e\u92c5\u6216\u5967\u9ea5\u4e01\u92c5\uff0c\u7c21\u7a31PTZ\uff0c\u5176\u5316\u5b78\u5f0f\u662fC10H8N2O2S2Zn\uff0c\u5176\u80fd\u4f5c\u70ba\u63a8\u52d5\u92c5\u96e2\u5b50\u7a7f\u900f\u7d30\u80de\u76ae\u819c\u800c\u9032\u5230\u7d30\u80de\u5167\u90e8\u53bb\u6291\u5236\u53ef\u652f\u6301\u75c5\u6bd2RNA\u8907\u88fd\u7684RdRps\u7684\u6d3b\u6027\uff0c\u4f9d\u64da \u91cd\u5fb7 \u7684\u7814\u7a76\u731c\u6e2c\u539f\u56e0\u4e3b\u8981\u662fPTZ\u70ba\u87af\u5408\u9ad4\uff0c\u4e26\u5177\u6709\u6975\u4f4e\u7684\u6eb6\u89e3\u5ea6\uff08\u7d048 ppm\uff09\uff0c\u4e5f\u5f88\u53ef\u80fd\u8207\u5176\u662f\u826f\u597d\u7684\u6bba\u771f\u83cc\u5291\u548c\u6bba\u85fb\u5291\u6709\u95dc\u3002 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\u91cd\u5fb7\u7684\u9019\u4e00\u500b\u8ad6\u9ede\u4e5f\u53ef\u4ee5\u7528\u65bc\u8aaa\u660e\u70ba\u4f55\u4f0a\u7dad\u83cc\u7d20\uff08ivermectin\uff09\u4e5f\u6709\u4e0d\u932f\u7684\u9632\u6cbbCOVID-19\u7684\u6548\u679c\uff0c\u56e0\u70ba\u4f0a\u7dad\u83cc\u7d20\u4e4b\u6240\u4ee5\u662f\u4e00\u7a2e\u5f88\u6709\u6548\u7684\u6bba\u87f2\u5291\uff0c\u662f\u56e0\u70ba\u5176\u7528\u6a5f\u5236\u662f\u4f7f\u589e\u52a0\u7121\u810a\u690e\u52d5\u7269\u7684\u7d30\u80de\u58c1\u53ef\u900f\u6027\uff0c\u56e0\u6b64\u5b83\u81ea\u7136\u4e5f\u53ef\u80fd\u6703\u7a7f\u900f\u53d7\u75c5\u6bd2\u611f\u67d3\u7684\u7d30\u80de\u58c1\u800c\u9032\u53bb\u6bba\u6b7b\u75c5\u6bd2\uff0c\u4f46\u662f\u9019\u662f\u4e00\u7a2e\u6bba\u622e\u65b9\u5f0f\uff0c\u5c0dSARS-CoV-2\u7684\u534a\u6291\u5236\u6fc3\u5ea6\u5927\u7d04\u70ba2.2\u20132.8\u03bcM\uff0c\u56e0\u6b64\u82e5\u8981\u5c0dCOVID-19\u7522\u751f\u6291\u5236\u6548\u679c\u5247\u5176\u6240\u9700\u5291\u91cf\u9060\u9ad8\u65bc\u4eba\u7528\u6700\u9ad8\u6279\u51c6\u5291\u91cf\u3001\u4ea6\u8d85\u904e\u53ef\u5b89\u5168\u5be6\u73fe\u5291\u91cf\u3002\u56e0\u6b64\u9060\u4e0d\u5982PTZ\u4f86\u7684\u5b89\u5168\u6709\u6548\u3002 \u3010\u8a0e\u8ad611\u3011 \u92c5\u96e2\u5b50\u5c0d\u6d41\u611f\u75c5\u6bd2\u8207SARS\u75c5\u6bd2\u7684\u6291\u5236\u4f5c\u7528\u6709\u4f55\u7570\u540c\u5462\uff1f \u5be6\u9a57\u8b49\u5be6\uff0c\u4f4e\u6fc3\u5ea6 Zn2+ \u548c PT\uff082 \u00b5M Zn2+ \u548c 2 \u00b5M PT\uff09\u7684\u7d44\u5408\u53ef\u6291\u5236\u7d30\u80de\u57f9\u990a\u4e2d SARS \u51a0\u72c0\u75c5\u6bd2 (SARS-CoV) \u548c\u99ac\u52d5\u8108\u708e\u75c5\u6bd2 (EAV) \u7684\u8907\u5236\u3002\u56e0\u70ba\u9019\u5169\u7a2e\u9060\u89aa\u5de2\u72c0\u75c5\u6bd2\u7684 RNA \u5408\u6210\u7531\u65bc\u4f9d\u8cf4\u65bc\u7d30\u80de\u5167\u539f\u6709\u7684 RNA \u805a\u5408\u9176 (RdRps) \u4f86\u50ac\u5316\uff0c\u56e0\u6b64\uff0cRdRps \u662f\u5176\u591a\u86cb\u767d\u8907\u88fd\u548c\u8f49\u9304\u8907\u5408\u7269 (RTC) \u7684\u6838\u5fc3\u9176\u3002\u800cZn2+ 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\u5305\u542b\uff1a\u4e8c\u9806\u53cd\u5b50\u75c5\u6bd2\u79d1\uff08Dicistroviridae\uff09\u3001\u50b3\u67d3\u6027\u8edf\u5316\u75c5\u6bd2\u79d1\uff08Iflaviridae\uff09\u3001\u6d77\u6d0bRNA\u75c5\u6bd2\u79d1\uff08Marnaviridae\uff09\u3001\u5fae\u5c0f\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u79d1\uff08Picornaviridae\uff09\u3001\u4f34\u751f\u8c47\u8c46\u75c5\u6bd2\u79d1(Secoviridae)\u3001\u8c47\u8c46\u82b1\u8449\u75c5\u6bd2\u4e9e\u79d1\uff08Comoviridae\uff09\u3001Bacillarnavirus\u3001Labyrnavirus\u3002 (3). \u856a\u83c1\u9ec3\u82b1\u8449\u75c5\u6bd2\u76ee(Tymovirales) \u5305\u542b\uff1a\u7532\u578b\u7dda\u5f62\u75c5\u6bd2\u79d1\uff08Alphaflexiviridae\uff09\u3001\u4e59\u578b\u7dda\u5f62\u75c5\u6bd2\u79d1(Betaflexiviridae)\u3001\u4e19\u578b\u7dda\u5f62\u75c5\u6bd2\u79d1(Gammaflexiviridae)\u3001\u856a\u83c1\u767c\u9ec3\u9472\u5d4c\u75c5\u6bd2\u79d1\uff08Tymoviridae\uff09\u3002 (4). \u672a\u8a2d\u76ee\u7684\u75c5\u6bd2\u79d1 \u5305\u542b\uff1aAlphatetraviridae\u3001\u8702\u7aa9\u72c0\u75c5\u6bd2\u79d1\uff08Alvernaviridae\uff09\u3001\u661f\u72c0\u75c5\u6bd2\u79d1\uff08Astroviridae\uff09\u3001\u687f\u83cc\u72c0\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u79d1\uff08Barnaviridae\uff09\u3001\u751c\u83dc\u58de\u6b7b\u9ec3\u8108\u75c5\u6bd2\u79d1\uff08Benyviridae\uff09\u3001\u96c0\u9ea5\u82b1\u8449\u75c5\u6bd2\u79d1\uff08Bromoviridae\uff09\u3001\u676f\u72c0\u75c5\u6bd2\u79d1\uff08Caliciviridae\uff09\uff1a\u5d4c\u676f\u75c5\u6bd2\u79d1\uff08Carmotetraviridae\uff09\u3001\u4fee\u9053\u9662\u75c5\u6bd2\u79d1\uff08Closteroviridae\uff09\u3001\u9ec3\u75c5\u6bd2\u79d1\uff08Flaviviridae\uff09\u3001\u809d\u708e\u75c5\u6bd2\u79d1\uff08Hepeviridae\uff09\u3001\u4f4e\u6bd2\u6027\u75c5\u6bd2\u79d1\uff08Hypoviridae\uff09\u3001\u5149\u6ed1\u75c5\u6bd2\u79d1\uff08Leviviridae\uff09\u3001\u9ec3\u75c7\u75c5\u6bd2\u79d1\uff08Luteoviridae\uff09\u3001\u88f8\u9732\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\uff08Narnaviridae)\u3001\u91ce\u7530\u75c5\u6bd2\u79d1\uff08Nodaviridae\uff09\u3001\u6d3e\u6c0f\u75c5\u6bd2\u79d1\uff08Permutotetraviridae\uff09\u3001\u99ac\u9234\u85afY\u75c5\u6bd2\u79d1\uff08Potyviridae\uff09\u3001\u62ab\u8863\u75c5\u6bd2\u79d1\uff08Togaviridae\uff09\u3001\u756a\u8304\u53e2\u77ee\u75c5\u6bd2\u79d1\uff08Tombusviridae\uff09\u3001\u5e1a\u72c0\u75c5\u6bd2\u79d1\uff08Virgaviridae\uff09\u7b49\u3002 \u3010\u8a0e\u8ad603\u3011 \u5728\u6b63\u93c8RNA\u75c5\u6bd2\u7684\u591a\u7a2e\u8907\u5236\u7b56\u7565\u4e2d\u5c45\u95dc\u9375\u56e0\u7d20\u8005\u70ba\u4f55\uff1f \u6b63\u93c8RNA (+RNA) \u75c5\u6bd2\u5305\u62ec\u8a31\u591a\u91cd\u8981\u7684\u75c5\u539f\u9ad4\u3002\u5b83\u5011\u5df2\u7d93\u9032\u5316\u51fa\u591a\u7a2e\u8907\u5236\u7b56\u7565\uff0c\u4f46\u7d71\u4e00\u7684\u4e8b\u5be6\u662f\u4f9d\u8cf4\u65bc\u7d30\u80de\u5167RNA\u5df2\u5b58\u5728\u7684RNA\u805a\u5408\u9176(RdRps)\u4f5c\u70ba\u75c5\u6bd2RNA\u5408\u6210\u6a5f\u5236\u7684\u6838\u5fc3\u9176\u3002\u800c\u4e14RdRp \u901a\u5e38\u5d4c\u5165\u5728\u7531\u75c5\u6bd2 RNA\u3001\u75c5\u6bd2\u548c\u5bbf\u4e3b\u86cb\u767d\u7d44\u88dd\u800c\u6210\u7684\u819c\u76f8\u95dc\u8907\u88fd\u8907\u5408\u7269\u4e2d\u3002\u56e0\u6b64\uff0c\u6709\u6548\u7684\u6291\u5236RdRps \u662f\u6297\u75c5\u6bd2\u7814\u7a76\u7684\u95dc\u9375\u76ee\u6a19\u3002\u3008View Article\u3009\u3002 \u91cd\u5fb7\u8a8d\u70ba\u7531\u65bc\u6b63\u93c8RNA (+RNA) 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\u4fe1\u865f\u5206\u5225\u964d\u4f4e\u4e86 98\u00b11% \u548c 85\u00b13%\u3002\u5c0d\u65bc PT \u548c ZnOAc2 \u6fc3\u5ea6\u7684\u9019\u7a2e\u7d44\u5408\uff0c\u4e5f\u6c92\u6709\u89c0\u5bdf\u5230\u7d30\u80de\u6bd2\u6027\u3002\u3008View Article\u3009 \u3010\u8a0e\u8ad610\u3011 \u4f55\u7a2e\u92c5\u96e2\u5b50\u8f09\u9ad4\u53ef\u4ee5\u6291\u5236 RNA \u75c5\u6bd2\u7684\u8907\u5236\u5462\uff1f \u5728\u7d30\u80de\u57f9\u990a\u7814\u7a76\u4e2d\uff0c\u6211\u5011\u767c\u73fe\u9ad8 Zn2+ \u6fc3\u5ea6\u548c\u6dfb\u52a0\u523a\u6fc0\u7d30\u80de\u8f38\u5165 Zn2+ \u7684\u5316\u5408\u7269\uff0c\u5982\u6a9c\u9187 (HK)\u3001\u5421\u54af\u70f7\u4e8c\u786b\u4ee3\u6c28\u57fa\u7532\u9178\u916f (PDTC) \u548c\u5421\u5576\u786b\u916e (PT)\uff0c\u53ef\u6291\u5236\u5404\u7a2e RNA \u75c5\u6bd2\u7684\u8907\u5236\uff0c\u5305\u62ec\u6d41\u611f\u75c5\u6bd2\u3001\u547c\u5438\u9053\u5408\u80de\u75c5\u6bd2\u548c\u5e7e\u7a2e\u5c0f\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u3002\u3008View Article\u3009\u3008View Article\u3009\u3008View Article\u3009\u3008View Article\u3009\u3008View Article\u3009\u3008View Article\u3009 \u5421\u5576\u786b\u916e(PT)\u8207\u92c5\u96e2\u5b50\u7684\u87af\u5408\u7269\u5c31\u7a31\u4f5c\u70ba\u5421\u5576\u786b\u916e\u92c5\u6216\u5967\u9ea5\u4e01\u92c5\uff0c\u7c21\u7a31PTZ\uff0c\u5176\u5316\u5b78\u5f0f\u662fC10H8N2O2S2Zn\uff0c\u5176\u80fd\u4f5c\u70ba\u63a8\u52d5\u92c5\u96e2\u5b50\u7a7f\u900f\u7d30\u80de\u76ae\u819c\u800c\u9032\u5230\u7d30\u80de\u5167\u90e8\u53bb\u6291\u5236\u53ef\u652f\u6301\u75c5\u6bd2RNA\u8907\u88fd\u7684RdRps\u7684\u6d3b\u6027\uff0c\u4f9d\u64da \u91cd\u5fb7 \u7684\u7814\u7a76\u731c\u6e2c\u539f\u56e0\u4e3b\u8981\u662fPTZ\u70ba\u87af\u5408\u9ad4\uff0c\u4e26\u5177\u6709\u6975\u4f4e\u7684\u6eb6\u89e3\u5ea6\uff08\u7d048 ppm\uff09\uff0c\u4e5f\u5f88\u53ef\u80fd\u8207\u5176\u662f\u826f\u597d\u7684\u6bba\u771f\u83cc\u5291\u548c\u6bba\u85fb\u5291\u6709\u95dc\u3002 \u91cd\u5fb7\u8a8d\u70ba\uff0c\u56e0\u70ba\u5176\u5177\u6709\u751a\u4f4e\u6eb6\u89e3\u5ea6\u4f7f\u5f97PTZ\u53ef\u4ee5\u5b8c\u6574\u7121\u640d\u7684\u5230\u9054\u7d30\u80de\u58c1\uff0c\u66f4\u56e0\u70ba\u5176\u5177\u6709\u6bba\u771f\u83cc\u8207\u6bba\u85fb\u7684\u5929\u6027\uff0c\u800c\u4f7f\u5f97PTZ\u6703\u5faa\u8457\u6a19\u9776\u75c5\u6bd2\u7684\u8ecc\u8de1\u53bb\u6bba\u83cc\u3002\u800c\u4e14PTZ\u5728\u5230\u9054\u88ab\u75c5\u6bd2\u4fb5\u5165\u7684\u7d30\u80de\u58c1\u6642\uff0c\u6703\u56e0\u70ba\u8981\u87af\u5408\u90a3\u4e00\u4e9b\u75c5\u6bd2\u7684\u6b98\u9ad4\u800c\u6703\u91cb\u653e\u51faZn2+\uff0c\u56e0\u6b64Zn2+ \u5c31\u81ea\u7136\u5faa\u8457\u75c5\u6bd2\u8ecc\u8de1\u800c\u7a7f\u900f\u7d30\u80de\u58c1\uff0c\u4e26\u9032\u5165\u7d30\u80de\u4f86\u6291\u5236RdRps\u7684\u6d3b\u6027\u3002 \u91cd\u5fb7\u7684\u9019\u4e00\u500b\u8ad6\u9ede\u4e5f\u53ef\u4ee5\u7528\u65bc\u8aaa\u660e\u70ba\u4f55\u4f0a\u7dad\u83cc\u7d20\uff08ivermectin\uff09\u4e5f\u6709\u4e0d\u932f\u7684\u9632\u6cbbCOVID-19\u7684\u6548\u679c\uff0c\u56e0\u70ba\u4f0a\u7dad\u83cc\u7d20\u4e4b\u6240\u4ee5\u662f\u4e00\u7a2e\u5f88\u6709\u6548\u7684\u6bba\u87f2\u5291\uff0c\u662f\u56e0\u70ba\u5176\u7528\u6a5f\u5236\u662f\u4f7f\u589e\u52a0\u7121\u810a\u690e\u52d5\u7269\u7684\u7d30\u80de\u58c1\u53ef\u900f\u6027\uff0c\u56e0\u6b64\u5b83\u81ea\u7136\u4e5f\u53ef\u80fd\u6703\u7a7f\u900f\u53d7\u75c5\u6bd2\u611f\u67d3\u7684\u7d30\u80de\u58c1\u800c\u9032\u53bb\u6bba\u6b7b\u75c5\u6bd2\uff0c\u4f46\u662f\u9019\u662f\u4e00\u7a2e\u6bba\u622e\u65b9\u5f0f\uff0c\u5c0dSARS-CoV-2\u7684\u534a\u6291\u5236\u6fc3\u5ea6\u5927\u7d04\u70ba2.2\u20132.8\u03bcM\uff0c\u56e0\u6b64\u82e5\u8981\u5c0dCOVID-19\u7522\u751f\u6291\u5236\u6548\u679c\u5247\u5176\u6240\u9700\u5291\u91cf\u9060\u9ad8\u65bc\u4eba\u7528\u6700\u9ad8\u6279\u51c6\u5291\u91cf\u3001\u4ea6\u8d85\u904e\u53ef\u5b89\u5168\u5be6\u73fe\u5291\u91cf\u3002\u56e0\u6b64\u9060\u4e0d\u5982PTZ\u4f86\u7684\u5b89\u5168\u6709\u6548\u3002 \u3010\u8a0e\u8ad611\u3011 \u92c5\u96e2\u5b50\u5c0d\u6d41\u611f\u75c5\u6bd2\u8207SARS\u75c5\u6bd2\u7684\u6291\u5236\u4f5c\u7528\u6709\u4f55\u7570\u540c\u5462\uff1f \u5be6\u9a57\u8b49\u5be6\uff0c\u4f4e\u6fc3\u5ea6 Zn2+ \u548c PT\uff082 \u00b5M Zn2+ \u548c 2 \u00b5M PT\uff09\u7684\u7d44\u5408\u53ef\u6291\u5236\u7d30\u80de\u57f9\u990a\u4e2d SARS \u51a0\u72c0\u75c5\u6bd2 (SARS-CoV) \u548c\u99ac\u52d5\u8108\u708e\u75c5\u6bd2 (EAV) \u7684\u8907\u5236\u3002\u56e0\u70ba\u9019\u5169\u7a2e\u9060\u89aa\u5de2\u72c0\u75c5\u6bd2\u7684 RNA \u5408\u6210\u7531\u65bc\u4f9d\u8cf4\u65bc\u7d30\u80de\u5167\u539f\u6709\u7684 RNA \u805a\u5408\u9176 (RdRps) \u4f86\u50ac\u5316\uff0c\u56e0\u6b64\uff0cRdRps \u662f\u5176\u591a\u86cb\u767d\u8907\u88fd\u548c\u8f49\u9304\u8907\u5408\u7269 (RTC) \u7684\u6838\u5fc3\u9176\u3002\u800cZn2+ \u88abPT\u87af\u5408\u5f8c\uff0c\u5c07\u5f88\u6709\u6548\u7387\u5730\u7a7f\u900f\u7d30\u80de\u58c1\u800c\u88ab\u9001\u9032\u7d30\u80de\u4e2d\u53bb\u6291\u5236RdRps\uff0c\u56e0\u6b64\u53ea\u8981\u67092 \u00b5M Zn2+ \u4fbf\u8db3\u4ee5\u9577\u671f\u6291\u5236RdRps\u7684\u6d3b\u6027\u4e86\u3002\u3008View Article\u3009 \u4f46\u662fZn2+ \u96d6\u7136\u6703\u963b\u65b7 EAV RNA \u5408\u6210\u7684\u8d77\u59cb\u6b65\u9a5f\uff0c\u4f46\u662f\u5c0d\u65bc\u75c5\u6bd2\u5ef6\u4f38\u671f\u7684\u6291\u5236\u6548\u679c\u4e26\u6c92\u6709\u90a3\u9ebc\u7406\u60f3\u3002\u3008View Article\u3009 EAV-GFP \u7de8\u78bc GFP \u8207\u75c5\u6bd2\u975e\u7d50\u69cb\u86cb\u767d 2 (nsp2) \u7684 N \u7aef\u878d\u5408\uff0c\u75c5\u6bd2\u975e\u7d50\u69cb\u86cb\u767d 2 (nsp2) \u662f\u8907\u5236\u9176\u591a\u86cb\u767d\u7684\u5207\u5272\u7522\u7269\u4e4b\u4e00\uff0c\u56e0\u6b64\u70ba\u8907\u5236\u9176\u57fa\u56e0\u7684\u7ffb\u8b6f\u63d0\u4f9b\u76f4\u63a5\u8b80\u6578\u3002\u3008View Article\u3009 \u9019\u662f\u56e0\u70ba\u5206\u6bb5\u8ca0\u93c8 RNA \u75c5\u6bd2\uff08\u4f8b\u5982\u6d41\u611f\u75c5\u6bd2\uff09\u7684\u8907\u5236\u4e0d\u4f9d\u8cf4\u65bc\u591a\u86cb\u767d\u52a0\u5de5\uff0c\u56e0\u6b64PDTC\u4ecb\u5c0e\u7684Zn2+ \u8f38\u5165\u7684\u5f71\u97ffEAV RNA \u5408\u6210\u7684\u8d77\u59cb\u6b65\uff0c\u5176\u5be6\u662f\u7531\u65bc Zn2+ \u6291\u5236\u4e86\u75c5\u6bd2 RNA\u4f9d\u8cf4\u6027RNA\u805a\u5408\u9176(RdRp)\u548c\u7d30\u80de\u8f14\u52a9\u56e0\u5b50\uff0c\u4f46\u662f\u4e00\u65e6\u767c\u5c55\u51fa\u8ca0\u93c8 RNA \u75c5\u6bd2\u5f8c\u4fbf\u518d\u7531\u8ca0\u80a1\u7522\u751f\u51fa\u8a31\u591a\u65b0\u7684RNA\uff08\u6b63\u80a1\uff09\uff0c\u6210\u70ba\u8907\u88fd\u4e2d\u9593\u9ad4\u3002\u6b64\u65b0\u7684\u6b63\u80a1RNA\u53ef\u4f5c\u70bamRNA\u5408\u6210\u8863\u6bbc\u86cb\u767d(\u8acb\u53c3\u8003\u3010\u8a0e\u8ad601\u3011)\u3002\u3008View Article\u3009 \u7136\u800c\u5728 SARS-CoV \u7684\u60c5\u6cc1\u4e0b\uff0cRdRps \u7684\u5ef6\u4f38\u4e00\u76f4\u53d7\u5230Zn2+ \u6291\u5236\uff0c\u56e0\u6b64\u6a21\u677f\u7684\u7d50\u5408\u6e1b\u5c11\u3002\u3008View Article\u3009 \u5728 SARS-CoV-GFP \u4e2d\uff0c\u5831\u544a\u57fa\u56e0\u5f9e sg mRNA 7 \u958b\u59cb\u8868\u9054\uff0c\u5728\u66ff\u63db\u4e86\u5169\u500b\u8f14\u52a9\u86cb\u767d\u7de8\u78bc\u57fa\u56e0\uff08ORF 7a \u548c 7b\uff09\u4e4b\u5f8c\uff0c\u9019\u4e9b\u57fa\u56e0\u5c0d\u65bc\u7d30\u80de\u57f9\u990a\u4e2d\u7684\u8907\u88fd\u662f\u53ef\u6709\u53ef\u7121\u7684\u3002\u3008View Article\u3009 \u3010\u8a0e\u8ad612\u3011 \u70ba\u4f55MgEDTA\u53ef\u4ee5\u9006\u8f49\u92c5\u96e2\u5b50\u7684\u6291\u5236\u75c5\u6bd2\u4f5c\u7528\u5462\uff1f MgEDTA (\u4e59\u4e8c\u80fa\u56db\u4e59\u9178\u4e8c\u9209\u9382\uff09\u662f\u4e00\u7a2e\u87af\u5408\u5291\u53ef\u7528\u4ee5\u9006\u8f49 Zn2+ \u4ecb\u5c0e\u7684\u6291\u88fd\u4f5c\u7528\uff0c\u56e0\u70ba\u5b83\u5728\u91cb\u653e Mg2+ \u7684\u540c\u6642\u7279\u7570\u6027\u87af\u5408 Zn2+\uff0c\u9019\u662f\u7531\u65bc ZnEDTA 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\u6211\u5011\u6e2c\u8a66\u4e86 Zn2+ \u6dfb\u52a0\u5c0d RTC \u6d3b\u6027\u7684\u5f71\u97ff\u3002\u5c0d\u65bc EAV\u548c SARS-CoV\uff0c\u7576\u5b58\u5728 ZnOAc2 \u6642\uff0c\u89c0\u5bdf\u5230\u5408\u6210\u7684 RNA \u91cf\u5448\u5291\u91cf\u4f9d\u8cf4\u6027\u4e0b\u964d\u3002\u5c0d\u65bc\u9019\u5169\u7a2e\u75c5\u6bd2\uff0c\u5728 50 \u00b5M \u7684 Zn2+ \u6fc3\u5ea6\u4e0b\u89c0\u5bdf\u5230\u7e3d RNA \u5408\u6210\u6e1b\u5c11\u4e86 50% \u4ee5\u4e0a\uff0c\u800c\u5728 500 \u00b5M \u7684 Zn2+ \u6fc3\u5ea6\u4e0b\u4ecd\u4fdd\u6301\u4e0d\u5230 5% \u7684\u6d3b\u6027\u3002\u57fa\u56e0\u7d44\u5408\u6210\u548c sg mRNA \u7522\u751f\u540c\u6a23\u53d7\u5230\u5f71\u97ff\u3002\u3008View Article\u3009 \u3010\u8a0e\u8ad607\u3011 \u9069\u7576\u6fc3\u5ea6\u92c5\u96e2\u5b50\u5c0d\u54ea\u4e9b\u75c5\u6bd2\u7684\u8907\u88fd\u6709\u6291\u5236\u6548\u679c\u5462\uff1f 1. \u91dd\u5c0d\u5c0f\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u9032\u884c\u4e86\u5408\u7406\u8a73\u7d30\u7684\u7814\u7a76\u5f8c\u8b49\u660e\u5728\u7d30\u80de\u57f9\u990a\u4e2d\uff0cPT \u5728\u6578\u5206\u9418\u5167\u523a\u6fc0Zn2+\u651d\u53d6\u4e26\u901a\u904e\u4e00\u7a2e\u6a5f\u5236\u6291\u5236 RNA \u75c5\u6bd2\u8907\u88fd\u3002\u3008View Article\u3009\u3008View Article\u3009 2. \u5c0d\u7d14\u5316\u7684\u9f3b\u75c5\u6bd2\u548c\u810a\u9ad3\u7070\u8cea\u708e\u75c5\u6bd2 3C \u86cb\u767d\u9176\u9032\u884c\u7684\u9ad4\u5916\u7814\u7a76\u8868\u660e\uff0c\u86cb\u767d\u9176\u6d3b\u6027\u88ab Zn2+ \u6291\u5236\u3002\u3008View Article\u3009\u3008View Article\u3009 3. \u5728\u611f\u67d3\u4eba\u9f3b\u75c5\u6bd2\u548c\u67ef\u85a9\u5947\u75c5\u6bd2B3\u7684\u7d30\u80de\u4e2d\u89c0\u5bdf\u5230\u7684\u92c5\u96e2\u5b50\u5c0d\u591a\u86cb\u767d\u52a0\u5de5\u7684\u6291\u88fd\u4f5c\u7528\u3002\u3008View Article\u3009\u3008View Article\u3009 4. Zn2+\u5c0d\u4f86\u81ea\u9f3b\u75c5\u6bd2\u548c\u4e19\u578b\u809d\u708e\u75c5\u6bd2\u7684\u7d14\u5316RdRps\u7684\u6d3b\u6027\u6709\u6291\u88fd\u4f5c\u7528\u3002\u3008View Article\u3009\u3008View Article\u3009 5. \u92c5\u96e2\u5b50\u88ab\u8b49\u660e\u53ef\u4ee5\u6291\u5236\u611f\u67d3\u7d30\u80de\u548c\u7121\u7d30\u80de\u7cfb\u7d71\u4e2d\u51a0\u72c0\u75c5\u6bd2\u8907\u88fd\u9176\u591a\u86cb\u767d\u52a0\u5de5\u904e\u7a0b\u4e2d\u7684\u67d0\u4e9b\u86cb\u767d\u6c34\u89e3\u88c2\u89e3\u3002\u3008View Article\u3009\u3008View Article\u3009 \u3010\u8a0e\u8ad608\u3011 \u5728\u7f3a\u5c11\u5421\u5576\u786b\u916e(PT)\u60c5\u6cc1\u4e0b\u92c5\u96e2\u5b50\u80fd\u6291\u5236\u75c5\u6bd2\u8907\u88fd\u55ce\uff1f \u6211\u5011\u767c\u73fe\u55ae\u7368\u6dfb\u52a0 ZnOAc2 \u4e5f\u6e1b\u5c11\u4e86\u75c5\u6bd2\u8907\u88fd\uff0c\u4f46\u50c5\u5728\u63a5\u8fd1 Vero-E6 \u7d30\u80de\u4e2d ZnOAc2 \u7684 50% \u7d30\u80de\u6bd2\u6027\u6fc3\u5ea6\uff08CC50\uff09\u7684\u6c34\u5e73\uff08~70 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\u4fe1\u865f\u5206\u5225\u964d\u4f4e\u4e86 98\u00b11% \u548c 85\u00b13%\u3002\u5c0d\u65bc PT \u548c ZnOAc2 \u6fc3\u5ea6\u7684\u9019\u7a2e\u7d44\u5408\uff0c\u4e5f\u6c92\u6709\u89c0\u5bdf\u5230\u7d30\u80de\u6bd2\u6027\u3002\u3008View Article\u3009 \u3010\u8a0e\u8ad610\u3011 \u4f55\u7a2e\u92c5\u96e2\u5b50\u8f09\u9ad4\u53ef\u4ee5\u6291\u5236 RNA \u75c5\u6bd2\u7684\u8907\u5236\u5462\uff1f \u5728\u7d30\u80de\u57f9\u990a\u7814\u7a76\u4e2d\uff0c\u6211\u5011\u767c\u73fe\u9ad8 Zn2+ \u6fc3\u5ea6\u548c\u6dfb\u52a0\u523a\u6fc0\u7d30\u80de\u8f38\u5165 Zn2+ \u7684\u5316\u5408\u7269\uff0c\u5982\u6a9c\u9187 (HK)\u3001\u5421\u54af\u70f7\u4e8c\u786b\u4ee3\u6c28\u57fa\u7532\u9178\u916f (PDTC) \u548c\u5421\u5576\u786b\u916e (PT)\uff0c\u53ef\u6291\u5236\u5404\u7a2e RNA \u75c5\u6bd2\u7684\u8907\u5236\uff0c\u5305\u62ec\u6d41\u611f\u75c5\u6bd2\u3001\u547c\u5438\u9053\u5408\u80de\u75c5\u6bd2\u548c\u5e7e\u7a2e\u5c0f\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u3002\u3008View Article\u3009\u3008View Article\u3009\u3008View Article\u3009\u3008View Article\u3009\u3008View Article\u3009\u3008View Article\u3009 \u5421\u5576\u786b\u916e(PT)\u8207\u92c5\u96e2\u5b50\u7684\u87af\u5408\u7269\u5c31\u7a31\u4f5c\u70ba\u5421\u5576\u786b\u916e\u92c5\u6216\u5967\u9ea5\u4e01\u92c5\uff0c\u7c21\u7a31PTZ\uff0c\u5176\u5316\u5b78\u5f0f\u662fC10H8N2O2S2Zn\uff0c\u5176\u80fd\u4f5c\u70ba\u63a8\u52d5\u92c5\u96e2\u5b50\u7a7f\u900f\u7d30\u80de\u76ae\u819c\u800c\u9032\u5230\u7d30\u80de\u5167\u90e8\u53bb\u6291\u5236\u53ef\u652f\u6301\u75c5\u6bd2RNA\u8907\u88fd\u7684RdRps\u7684\u6d3b\u6027\uff0c\u4f9d\u64da \u91cd\u5fb7 \u7684\u7814\u7a76\u731c\u6e2c\u539f\u56e0\u4e3b\u8981\u662fPTZ\u70ba\u87af\u5408\u9ad4\uff0c\u4e26\u5177\u6709\u6975\u4f4e\u7684\u6eb6\u89e3\u5ea6\uff08\u7d048 ppm\uff09\uff0c\u4e5f\u5f88\u53ef\u80fd\u8207\u5176\u662f\u826f\u597d\u7684\u6bba\u771f\u83cc\u5291\u548c\u6bba\u85fb\u5291\u6709\u95dc\u3002 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\u91cd\u5fb7\u7684\u9019\u4e00\u500b\u8ad6\u9ede\u4e5f\u53ef\u4ee5\u7528\u65bc\u8aaa\u660e\u70ba\u4f55\u4f0a\u7dad\u83cc\u7d20\uff08ivermectin\uff09\u4e5f\u6709\u4e0d\u932f\u7684\u9632\u6cbbCOVID-19\u7684\u6548\u679c\uff0c\u56e0\u70ba\u4f0a\u7dad\u83cc\u7d20\u4e4b\u6240\u4ee5\u662f\u4e00\u7a2e\u5f88\u6709\u6548\u7684\u6bba\u87f2\u5291\uff0c\u662f\u56e0\u70ba\u5176\u7528\u6a5f\u5236\u662f\u4f7f\u589e\u52a0\u7121\u810a\u690e\u52d5\u7269\u7684\u7d30\u80de\u58c1\u53ef\u900f\u6027\uff0c\u56e0\u6b64\u5b83\u81ea\u7136\u4e5f\u53ef\u80fd\u6703\u7a7f\u900f\u53d7\u75c5\u6bd2\u611f\u67d3\u7684\u7d30\u80de\u58c1\u800c\u9032\u53bb\u6bba\u6b7b\u75c5\u6bd2\uff0c\u4f46\u662f\u9019\u662f\u4e00\u7a2e\u6bba\u622e\u65b9\u5f0f\uff0c\u5c0dSARS-CoV-2\u7684\u534a\u6291\u5236\u6fc3\u5ea6\u5927\u7d04\u70ba2.2\u20132.8\u03bcM\uff0c\u56e0\u6b64\u82e5\u8981\u5c0dCOVID-19\u7522\u751f\u6291\u5236\u6548\u679c\u5247\u5176\u6240\u9700\u5291\u91cf\u9060\u9ad8\u65bc\u4eba\u7528\u6700\u9ad8\u6279\u51c6\u5291\u91cf\u3001\u4ea6\u8d85\u904e\u53ef\u5b89\u5168\u5be6\u73fe\u5291\u91cf\u3002\u56e0\u6b64\u9060\u4e0d\u5982PTZ\u4f86\u7684\u5b89\u5168\u6709\u6548\u3002 \u3010\u8a0e\u8ad611\u3011 \u92c5\u96e2\u5b50\u5c0d\u6d41\u611f\u75c5\u6bd2\u8207SARS\u75c5\u6bd2\u7684\u6291\u5236\u4f5c\u7528\u6709\u4f55\u7570\u540c\u5462\uff1f \u5be6\u9a57\u8b49\u5be6\uff0c\u4f4e\u6fc3\u5ea6 Zn2+ \u548c PT\uff082 \u00b5M Zn2+ \u548c 2 \u00b5M PT\uff09\u7684\u7d44\u5408\u53ef\u6291\u5236\u7d30\u80de\u57f9\u990a\u4e2d SARS \u51a0\u72c0\u75c5\u6bd2 (SARS-CoV) \u548c\u99ac\u52d5\u8108\u708e\u75c5\u6bd2 (EAV) \u7684\u8907\u5236\u3002\u56e0\u70ba\u9019\u5169\u7a2e\u9060\u89aa\u5de2\u72c0\u75c5\u6bd2\u7684 RNA \u5408\u6210\u7531\u65bc\u4f9d\u8cf4\u65bc\u7d30\u80de\u5167\u539f\u6709\u7684 RNA \u805a\u5408\u9176 (RdRps) \u4f86\u50ac\u5316\uff0c\u56e0\u6b64\uff0cRdRps \u662f\u5176\u591a\u86cb\u767d\u8907\u88fd\u548c\u8f49\u9304\u8907\u5408\u7269 (RTC) \u7684\u6838\u5fc3\u9176\u3002\u800cZn2+ 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\u5305\u542b\uff1aAlphatetraviridae\u3001\u8702\u7aa9\u72c0\u75c5\u6bd2\u79d1\uff08Alvernaviridae\uff09\u3001\u661f\u72c0\u75c5\u6bd2\u79d1\uff08Astroviridae\uff09\u3001\u687f\u83cc\u72c0\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u79d1\uff08Barnaviridae\uff09\u3001\u751c\u83dc\u58de\u6b7b\u9ec3\u8108\u75c5\u6bd2\u79d1\uff08Benyviridae\uff09\u3001\u96c0\u9ea5\u82b1\u8449\u75c5\u6bd2\u79d1\uff08Bromoviridae\uff09\u3001\u676f\u72c0\u75c5\u6bd2\u79d1\uff08Caliciviridae\uff09\uff1a\u5d4c\u676f\u75c5\u6bd2\u79d1\uff08Carmotetraviridae\uff09\u3001\u4fee\u9053\u9662\u75c5\u6bd2\u79d1\uff08Closteroviridae\uff09\u3001\u9ec3\u75c5\u6bd2\u79d1\uff08Flaviviridae\uff09\u3001\u809d\u708e\u75c5\u6bd2\u79d1\uff08Hepeviridae\uff09\u3001\u4f4e\u6bd2\u6027\u75c5\u6bd2\u79d1\uff08Hypoviridae\uff09\u3001\u5149\u6ed1\u75c5\u6bd2\u79d1\uff08Leviviridae\uff09\u3001\u9ec3\u75c7\u75c5\u6bd2\u79d1\uff08Luteoviridae\uff09\u3001\u88f8\u9732\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\uff08Narnaviridae)\u3001\u91ce\u7530\u75c5\u6bd2\u79d1\uff08Nodaviridae\uff09\u3001\u6d3e\u6c0f\u75c5\u6bd2\u79d1\uff08Permutotetraviridae\uff09\u3001\u99ac\u9234\u85afY\u75c5\u6bd2\u79d1\uff08Potyviridae\uff09\u3001\u62ab\u8863\u75c5\u6bd2\u79d1\uff08Togaviridae\uff09\u3001\u756a\u8304\u53e2\u77ee\u75c5\u6bd2\u79d1\uff08Tombusviridae\uff09\u3001\u5e1a\u72c0\u75c5\u6bd2\u79d1\uff08Virgaviridae\uff09\u7b49\u3002 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\u75c5\u6bd2\u4e26\u4e0d\u81ea\u5df1\u64c1\u6709RNA\u8907\u88fd\u9176\uff0c\u56e0\u6b64\u5b83\u53ea\u80fd\u5728\u9032\u5165\u7d30\u80de\u5f8c\u5229\u7528\u7d30\u80de\u5167\u539f\u6709\u7684RNA RNA\u8907\u88fd\u9176\u4f86\u4fc3\u9032\u75c5\u6bd2\u7684\u8907\u88fd\uff0c\u800c\u76ee\u524d\u5927\u591a\u6578\u5c0d\u6297COVID-19\u7684\u75ab\u82d7\u8a2d\u8a08\u90fd\u662f\u4fc3\u4f7f\u4eba\u9ad4\u7684\u514d\u75ab\u7cfb\u7d71\u5c0d\u65bcCOVID-19\u75c5\u6bd2\u6709\u201c\u53d7\u50b7\u7d93\u9a57\u201d\uff0c\u800c\u4fc3\u4f7f\u7d30\u80de\u5728\u9047\u5230\u5916\u578b\u8207COVID-19\u75c5\u6bd2\u96f7\u540c\u7684\u5916\u4f86\u8005\u6642\u201c\u9589\u9580\u8b1d\u5ba2\u201d\u3002 \u91cd\u5fb7\u8a8d\u70ba\u9019\u6a23\u7684\u75ab\u82d7\u8a2d\u8a08\u6703\u7522\u751f\u4e86\u4ee5\u4e0b\u5e7e\u500b\u76f2\u5340\uff1a 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Zn2+\u5c0d\u4f86\u81ea\u9f3b\u75c5\u6bd2\u548c\u4e19\u578b\u809d\u708e\u75c5\u6bd2\u7684\u7d14\u5316RdRps\u7684\u6d3b\u6027\u6709\u6291\u88fd\u4f5c\u7528\u3002\u3008View Article\u3009\u3008View Article\u3009 5. \u92c5\u96e2\u5b50\u88ab\u8b49\u660e\u53ef\u4ee5\u6291\u5236\u611f\u67d3\u7d30\u80de\u548c\u7121\u7d30\u80de\u7cfb\u7d71\u4e2d\u51a0\u72c0\u75c5\u6bd2\u8907\u88fd\u9176\u591a\u86cb\u767d\u52a0\u5de5\u904e\u7a0b\u4e2d\u7684\u67d0\u4e9b\u86cb\u767d\u6c34\u89e3\u88c2\u89e3\u3002\u3008View Article\u3009\u3008View Article\u3009 \u3010\u8a0e\u8ad608\u3011 \u5728\u7f3a\u5c11\u5421\u5576\u786b\u916e(PT)\u60c5\u6cc1\u4e0b\u92c5\u96e2\u5b50\u80fd\u6291\u5236\u75c5\u6bd2\u8907\u88fd\u55ce\uff1f \u6211\u5011\u767c\u73fe\u55ae\u7368\u6dfb\u52a0 ZnOAc2 \u4e5f\u6e1b\u5c11\u4e86\u75c5\u6bd2\u8907\u88fd\uff0c\u4f46\u50c5\u5728\u63a5\u8fd1 Vero-E6 \u7d30\u80de\u4e2d ZnOAc2 \u7684 50% \u7d30\u80de\u6bd2\u6027\u6fc3\u5ea6\uff08CC50\uff09\u7684\u6c34\u5e73\uff08~70 \u00b5M\uff0c\u6578\u64da\u672a\u986f\u793a\uff09\u3002\u9019\u53ef\u80fd\u662f\u7531\u65bc Zn2+ \u5728\u542b\u78f7\u9178\u9e7d\u7684\u57f9\u990a\u57fa\u4e2d\u6eb6\u89e3\u5ea6\u5dee\uff0c\u4ee5\u53ca\u5728\u6c92\u6709\u92c5\u96e2\u5b50\u8f09\u9ad4\u7684\u60c5\u6cc1\u4e0b\u7d30\u80de\u5c0d Zn2+ \u7684\u5438\u6536\u6548\u7387\u4f4e\u4e0b\u3002\u3008View Article\u3009 \u3010\u8a0e\u8ad609\u3011 \u55ae\u7528\u5421\u5576\u786b\u916e(PT)\u8207\u4f75\u7528\u92c5\u96e2\u5b50\u7684\u6548\u679c\u6709\u4f55\u5dee\u7570\u5462\uff1f \u901a\u904e\u55ae\u7368\u6dfb\u52a0 PT\uff0cSARS-CoV-GFP \u548c EAV-GFP \u7684\u5831\u544a\u57fa\u56e0\u8868\u9054\u5df2\u7d93\u4ee5\u5291\u91cf\u4f9d\u8cf4\u6027\u65b9\u5f0f\u986f\u8457\u6291\u5236\u3002\u7576\u5411\u57f9\u990a\u57fa\u4e2d\u52a0\u5165 2 \u00b5M Zn2+ \u6642\uff0c\u9019\u7a2e\u6548\u679c\u6703\u986f\u8457\u589e\u5f37\u3002 \u4f46\u662f2 \u00b5M PT \u548c 2 \u00b5M ZnOAc2 \u7684\u7d44\u5408\u4f7f EAV-GFP \u548c SARS-CoV-GFP \u7684 GFP \u4fe1\u865f\u5206\u5225\u964d\u4f4e\u4e86 98\u00b11% \u548c 85\u00b13%\u3002\u5c0d\u65bc PT \u548c ZnOAc2 \u6fc3\u5ea6\u7684\u9019\u7a2e\u7d44\u5408\uff0c\u4e5f\u6c92\u6709\u89c0\u5bdf\u5230\u7d30\u80de\u6bd2\u6027\u3002\u3008View Article\u3009 \u3010\u8a0e\u8ad610\u3011 \u4f55\u7a2e\u92c5\u96e2\u5b50\u8f09\u9ad4\u53ef\u4ee5\u6291\u5236 RNA \u75c5\u6bd2\u7684\u8907\u5236\u5462\uff1f \u5728\u7d30\u80de\u57f9\u990a\u7814\u7a76\u4e2d\uff0c\u6211\u5011\u767c\u73fe\u9ad8 Zn2+ \u6fc3\u5ea6\u548c\u6dfb\u52a0\u523a\u6fc0\u7d30\u80de\u8f38\u5165 Zn2+ \u7684\u5316\u5408\u7269\uff0c\u5982\u6a9c\u9187 (HK)\u3001\u5421\u54af\u70f7\u4e8c\u786b\u4ee3\u6c28\u57fa\u7532\u9178\u916f (PDTC) \u548c\u5421\u5576\u786b\u916e (PT)\uff0c\u53ef\u6291\u5236\u5404\u7a2e RNA \u75c5\u6bd2\u7684\u8907\u5236\uff0c\u5305\u62ec\u6d41\u611f\u75c5\u6bd2\u3001\u547c\u5438\u9053\u5408\u80de\u75c5\u6bd2\u548c\u5e7e\u7a2e\u5c0f\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u3002\u3008View Article\u3009\u3008View Article\u3009\u3008View Article\u3009\u3008View Article\u3009\u3008View Article\u3009\u3008View Article\u3009 \u5421\u5576\u786b\u916e(PT)\u8207\u92c5\u96e2\u5b50\u7684\u87af\u5408\u7269\u5c31\u7a31\u4f5c\u70ba\u5421\u5576\u786b\u916e\u92c5\u6216\u5967\u9ea5\u4e01\u92c5\uff0c\u7c21\u7a31PTZ\uff0c\u5176\u5316\u5b78\u5f0f\u662fC10H8N2O2S2Zn\uff0c\u5176\u80fd\u4f5c\u70ba\u63a8\u52d5\u92c5\u96e2\u5b50\u7a7f\u900f\u7d30\u80de\u76ae\u819c\u800c\u9032\u5230\u7d30\u80de\u5167\u90e8\u53bb\u6291\u5236\u53ef\u652f\u6301\u75c5\u6bd2RNA\u8907\u88fd\u7684RdRps\u7684\u6d3b\u6027\uff0c\u4f9d\u64da \u91cd\u5fb7 \u7684\u7814\u7a76\u731c\u6e2c\u539f\u56e0\u4e3b\u8981\u662fPTZ\u70ba\u87af\u5408\u9ad4\uff0c\u4e26\u5177\u6709\u6975\u4f4e\u7684\u6eb6\u89e3\u5ea6\uff08\u7d048 ppm\uff09\uff0c\u4e5f\u5f88\u53ef\u80fd\u8207\u5176\u662f\u826f\u597d\u7684\u6bba\u771f\u83cc\u5291\u548c\u6bba\u85fb\u5291\u6709\u95dc\u3002 \u91cd\u5fb7\u8a8d\u70ba\uff0c\u56e0\u70ba\u5176\u5177\u6709\u751a\u4f4e\u6eb6\u89e3\u5ea6\u4f7f\u5f97PTZ\u53ef\u4ee5\u5b8c\u6574\u7121\u640d\u7684\u5230\u9054\u7d30\u80de\u58c1\uff0c\u66f4\u56e0\u70ba\u5176\u5177\u6709\u6bba\u771f\u83cc\u8207\u6bba\u85fb\u7684\u5929\u6027\uff0c\u800c\u4f7f\u5f97PTZ\u6703\u5faa\u8457\u6a19\u9776\u75c5\u6bd2\u7684\u8ecc\u8de1\u53bb\u6bba\u83cc\u3002\u800c\u4e14PTZ\u5728\u5230\u9054\u88ab\u75c5\u6bd2\u4fb5\u5165\u7684\u7d30\u80de\u58c1\u6642\uff0c\u6703\u56e0\u70ba\u8981\u87af\u5408\u90a3\u4e00\u4e9b\u75c5\u6bd2\u7684\u6b98\u9ad4\u800c\u6703\u91cb\u653e\u51faZn2+\uff0c\u56e0\u6b64Zn2+ \u5c31\u81ea\u7136\u5faa\u8457\u75c5\u6bd2\u8ecc\u8de1\u800c\u7a7f\u900f\u7d30\u80de\u58c1\uff0c\u4e26\u9032\u5165\u7d30\u80de\u4f86\u6291\u5236RdRps\u7684\u6d3b\u6027\u3002 \u91cd\u5fb7\u7684\u9019\u4e00\u500b\u8ad6\u9ede\u4e5f\u53ef\u4ee5\u7528\u65bc\u8aaa\u660e\u70ba\u4f55\u4f0a\u7dad\u83cc\u7d20\uff08ivermectin\uff09\u4e5f\u6709\u4e0d\u932f\u7684\u9632\u6cbbCOVID-19\u7684\u6548\u679c\uff0c\u56e0\u70ba\u4f0a\u7dad\u83cc\u7d20\u4e4b\u6240\u4ee5\u662f\u4e00\u7a2e\u5f88\u6709\u6548\u7684\u6bba\u87f2\u5291\uff0c\u662f\u56e0\u70ba\u5176\u7528\u6a5f\u5236\u662f\u4f7f\u589e\u52a0\u7121\u810a\u690e\u52d5\u7269\u7684\u7d30\u80de\u58c1\u53ef\u900f\u6027\uff0c\u56e0\u6b64\u5b83\u81ea\u7136\u4e5f\u53ef\u80fd\u6703\u7a7f\u900f\u53d7\u75c5\u6bd2\u611f\u67d3\u7684\u7d30\u80de\u58c1\u800c\u9032\u53bb\u6bba\u6b7b\u75c5\u6bd2\uff0c\u4f46\u662f\u9019\u662f\u4e00\u7a2e\u6bba\u622e\u65b9\u5f0f\uff0c\u5c0dSARS-CoV-2\u7684\u534a\u6291\u5236\u6fc3\u5ea6\u5927\u7d04\u70ba2.2\u20132.8\u03bcM\uff0c\u56e0\u6b64\u82e5\u8981\u5c0dCOVID-19\u7522\u751f\u6291\u5236\u6548\u679c\u5247\u5176\u6240\u9700\u5291\u91cf\u9060\u9ad8\u65bc\u4eba\u7528\u6700\u9ad8\u6279\u51c6\u5291\u91cf\u3001\u4ea6\u8d85\u904e\u53ef\u5b89\u5168\u5be6\u73fe\u5291\u91cf\u3002\u56e0\u6b64\u9060\u4e0d\u5982PTZ\u4f86\u7684\u5b89\u5168\u6709\u6548\u3002 \u3010\u8a0e\u8ad611\u3011 \u92c5\u96e2\u5b50\u5c0d\u6d41\u611f\u75c5\u6bd2\u8207SARS\u75c5\u6bd2\u7684\u6291\u5236\u4f5c\u7528\u6709\u4f55\u7570\u540c\u5462\uff1f \u5be6\u9a57\u8b49\u5be6\uff0c\u4f4e\u6fc3\u5ea6 Zn2+ \u548c PT\uff082 \u00b5M Zn2+ \u548c 2 \u00b5M PT\uff09\u7684\u7d44\u5408\u53ef\u6291\u5236\u7d30\u80de\u57f9\u990a\u4e2d SARS \u51a0\u72c0\u75c5\u6bd2 (SARS-CoV) \u548c\u99ac\u52d5\u8108\u708e\u75c5\u6bd2 (EAV) \u7684\u8907\u5236\u3002\u56e0\u70ba\u9019\u5169\u7a2e\u9060\u89aa\u5de2\u72c0\u75c5\u6bd2\u7684 RNA \u5408\u6210\u7531\u65bc\u4f9d\u8cf4\u65bc\u7d30\u80de\u5167\u539f\u6709\u7684 RNA \u805a\u5408\u9176 (RdRps) \u4f86\u50ac\u5316\uff0c\u56e0\u6b64\uff0cRdRps \u662f\u5176\u591a\u86cb\u767d\u8907\u88fd\u548c\u8f49\u9304\u8907\u5408\u7269 (RTC) \u7684\u6838\u5fc3\u9176\u3002\u800cZn2+ \u88abPT\u87af\u5408\u5f8c\uff0c\u5c07\u5f88\u6709\u6548\u7387\u5730\u7a7f\u900f\u7d30\u80de\u58c1\u800c\u88ab\u9001\u9032\u7d30\u80de\u4e2d\u53bb\u6291\u5236RdRps\uff0c\u56e0\u6b64\u53ea\u8981\u67092 \u00b5M Zn2+ \u4fbf\u8db3\u4ee5\u9577\u671f\u6291\u5236RdRps\u7684\u6d3b\u6027\u4e86\u3002\u3008View Article\u3009 \u4f46\u662fZn2+ \u96d6\u7136\u6703\u963b\u65b7 EAV RNA \u5408\u6210\u7684\u8d77\u59cb\u6b65\u9a5f\uff0c\u4f46\u662f\u5c0d\u65bc\u75c5\u6bd2\u5ef6\u4f38\u671f\u7684\u6291\u5236\u6548\u679c\u4e26\u6c92\u6709\u90a3\u9ebc\u7406\u60f3\u3002\u3008View Article\u3009 EAV-GFP \u7de8\u78bc GFP \u8207\u75c5\u6bd2\u975e\u7d50\u69cb\u86cb\u767d 2 (nsp2) \u7684 N \u7aef\u878d\u5408\uff0c\u75c5\u6bd2\u975e\u7d50\u69cb\u86cb\u767d 2 (nsp2) \u662f\u8907\u5236\u9176\u591a\u86cb\u767d\u7684\u5207\u5272\u7522\u7269\u4e4b\u4e00\uff0c\u56e0\u6b64\u70ba\u8907\u5236\u9176\u57fa\u56e0\u7684\u7ffb\u8b6f\u63d0\u4f9b\u76f4\u63a5\u8b80\u6578\u3002\u3008View Article\u3009 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