{"id":104514,"date":"2022-05-27T15:08:21","date_gmt":"2022-05-27T15:08:21","guid":{"rendered":"http:\/\/pingtungtimes.com.tw\/?p=104514"},"modified":"2022-05-27T15:08:21","modified_gmt":"2022-05-27T15:08:21","slug":"%e5%a5%bd%e9%8b%85%e3%80%80%e4%b9%9f%e6%9c%83%e6%83%b9%e4%ba%86%e4%b8%80%e8%ba%ab%e8%85%a5","status":"publish","type":"post","link":"https:\/\/pingtungtimes.com.tw\/?p=104514","title":{"rendered":"\u597d\u201c\u92c5\u201d\u3000\u4e5f\u6703\u60f9\u4e86\u4e00\u8eab\u8165"},"content":{"rendered":"

\u3010\u674e\u91cd\u5fb7\uff0f\u5c08\u6b04\u5831\u5c0e2022\/05\/27\u3011\u3000\u4eca\u65e9\uff0c\u6709\u4e00\u78ba\u8a3a\u7652\u5f8c\u8005\u8207\u91cd\u5fb7\u8a0e\u8ad6\u4ed6\u7684\u75c5\u60c5\uff0c\u91cd\u5fb7\u5efa\u8b70\u4ed6\u88dc\u5145\u92c5\u96e2\u5b50\u98df\u54c1\uff0c\u4e26\u5c07\u6b64\u4e00\u4fe1\u606f\u4e5f\u767c\u7d66\u5927\u5bb6\u77e5\u66c9\uff1a<\/p>\n

\u300e\u6709\u78ba\u8a3a\u7652\u5f8c\u8005\u554f\u91cd\u5fb7\u8981\u5982\u4f55\u4fdd\u5065\u4ee5\u9632\u5fa9\u967d\uff0c\u91cd\u5fb7 \u7686\u5efa\u8b70\u4ed6\u5403\u3010\u92c5\u7cd6\u3011(\u985e\u4f3c\u5589\u7247)\u5c0f\u5b69\u5247\u5403\u3010\u5152\u7ae5\u92c5\u8edf\u7cd6\u3011\uff0c\u6216\u662f\u3010\u9175\u6bcd\u92c5\u3011\u3001\u3010\u87af\u5408\u92c5\u3011\u6216\u662f\u3010\u8461\u8404\u7cd6\u9178\u92c5\u3011\u4f86\u6d88\u6ec5\u6f5b\u85cf\u4eba\u9ad4\u5c1a\u672a\u767c\u4f5c\u7684\u75c5\u6bd2\u3002\u300f<\/p>\n

\u5f8c\u4f86\u5c31\u63a5\u7372\u4e00\u4f4d\u738b\u59d3\u7db2\u53cb\u56de\u8986\u8aaa\uff1a\u300e\u4e0d\u5c0d\u5594\uff01\u92c5,\u4eba\u985e\u5b58\u6d3b\u6240\u9700\u8981\u7684\u5143\u7d20\u4e4b\u4e00,\u5f9e\u5929\u7136\u98df\u7269\u76f4\u63a5\u651d\u53d6\u5c31\u8db3\u5920\u4e86,\u4eba\u70ba\u8d85\u91cf\u7684\u88dc\u5145\u6703\u51fa\u554f\u984c\u7684\uff01\u92c5,\u4e0d\u6703\u76f4\u63a5\u6bba\u75c5\u6bd2\u7684\uff01\u300f<\/p>\n

\u9019\u4e00\u4f4d\u738b\u5148\u751f\uff0c\u5927\u6982\u6f0f\u770b\u4e86\u91cd\u5fb7\u5728\u4e00\u5e74\u524d\u5728\u672c\u6703\u7fa4\u7d44\u4e0a\u767c\u8868\u7684\u5e7e\u7bc7\u4ee5\u92c5\u96e2\u5b50\u4f86\u6d88\u6ec5\u75c5\u6bd2\u7684\u6587\u7ae0\uff0c\u56e0\u6b64\uff0c\u91cd\u5fb7\u5c31\u56de\u8986\u4ed6\u8aaa\uff1a\u300e\u6211\u770b\u904e\u4e86\u8a31\u591a\u82f1\u6587\u91ab\u5b78\u6587\u737b\uff0c\u4e5f\u8207\u9ec3\u25cb\u5bcc\u535a\u58eb\u548c\u9673\u25cb\u5e73\u91ab\u5e2b\u8a0e\u8ad6\u904e\u3002\u60a8\u5982\u679c\u60f3\u8981\u770b\u9019\u4e00\u4e9b\u82f1\u6587\u91ab\u5b78\u6587\u737b\uff0c\u6211\u53ef\u4ee5\u5bc4\u53bb\u7d66\u60a8\u3002\u300f\u7136\u5f8c \u91cd\u5fb7 \u5c31\u5c31\u5bc4\u53bb\u7d66\u4ed6\u4ee5\u4e0b\u7684\u8cc7\u6599\uff1a<\/p>\n

References\u53c3\u8003\u6587\u737b<\/p>\n

1.Lazarczyk M, Favre M (2008) Role of Zn2+ ions in host-virus interactions. J Virol 82: 11486\u201311494.\u00a0\u00a0\u00a0 \u30081. Lazarczyk M, Favre M (2008) Zn2+ \u96e2\u5b50\u5728\u5bbf\u4e3b-\u75c5\u6bd2\u76f8\u4e92\u4f5c\u7528\u4e2d\u7684\u4f5c\u7528\u3002 J Virol 82\uff1a11486\u201311494\u3002\u3009\u3000View Article\u3000Google Scholar<\/p>\n

2.Frederickson CJ, Koh JY, Bush AI (2005) Neurobiology of zinc in health and disease. Nat Rev Neurosci 6: 449\u2013462.\u00a0\u00a0\u00a0 \u30082. Frederickson CJ\u3001Koh JY\u3001Bush AI (2005) \u92c5\u5728\u5065\u5eb7\u548c\u75be\u75c5\u4e2d\u7684\u795e\u7d93\u751f\u7269\u5b78\u3002 Nat Rev Neurosci 6\uff1a449-462\u3002\u3009View Article\u3000Google Scholar<\/p>\n

3.Alirezaei M, Nairn AC, Glowinski J, Premont J, Marin P (1999) Zinc inhibits protein synthesis in neurons: potential rol of phosphorylation of translation initiation factor-2a. J Biol Chem 274: 32433\u201332438.\u00a0\u00a0\u00a0 \u30083. Alirezaei M\u3001Nairn AC\u3001Glowinski J\u3001Premont J\u3001Marin P (1999) \u92c5\u6291\u5236\u795e\u7d93\u5143\u4e2d\u7684\u86cb\u767d\u8cea\u5408\u6210\uff1a\u7ffb\u8b6f\u8d77\u59cb\u56e0\u5b50 2a \u78f7\u9178\u5316\u7684\u6f5b\u5728\u4f5c\u7528\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 274\uff1a32433\u201332438\u3002\u3009View Article\u3000Google Scholar<\/p>\n

4.Uchide N, Ohyama K, Bessho T, Yuan B, Yamakawa T (2002) Effect of antioxidants on apoptosis induced by influenza virus infection: inhibition of viral gene replication and transcription with pyrrolidine dithiocarbamate. Antiviral Res 56: 207\u2013217.\u00a0\u00a0\u00a0 \u30084. Uchide N, Ohyama K, Bessho T, Yuan B, Yamakawa T (2002) \u6297\u6c27\u5316\u5291\u5c0d\u6d41\u611f\u75c5\u6bd2\u611f\u67d3\u8a98\u5c0e\u7684\u7d30\u80de\u51cb\u4ea1\u7684\u5f71\u97ff\uff1a\u7528\u5421\u54af\u70f7\u4e8c\u786b\u4ee3\u6c28\u57fa\u7532\u9178\u916f\u6291\u5236\u75c5\u6bd2\u57fa\u56e0\u8907\u5236\u548c\u8f49\u9304\u3002\u6297\u75c5\u6bd2\u7814\u7a76 56\uff1a207-217\u3002\u3009View Article\u3000Google Scholar<\/p>\n

5.Suara RO, Crowe JEJ (2004) Effect of zinc salts on respiratory syncytial virus replication. Antimicrob Agents Chemother 48: 783\u2013790.\u00a0\u00a0\u00a0 \u30085. Suara RO, Crowe JEJ (2004) \u92c5\u9e7d\u5c0d\u547c\u5438\u9053\u5408\u80de\u75c5\u6bd2\u8907\u88fd\u7684\u5f71\u97ff\u3002\u6297\u5fae\u751f\u7269\u5291 Chemother 48\uff1a783-790\u3002\u3009View Article\u3000Google Scholar<\/p>\n

6.Gaudernak E, Seipelt J, Triendl A, Grassauer A, Kuechler E (2002) Antiviral Effects of Pyrrolidine Dithiocarbamate on Human Rhinoviruses. J Virol 76: 6004\u20136015.\u00a0\u00a0\u00a0 \u30086. Gaudernak E\u3001Seipelt J\u3001Triendl A\u3001Grassauer A\u3001Kuechler E (2002) \u5421\u54af\u70f7\u4e8c\u786b\u4ee3\u6c28\u57fa\u7532\u9178\u916f\u5c0d\u4eba\u9f3b\u75c5\u6bd2\u7684\u6297\u75c5\u6bd2\u4f5c\u7528\u3002 J Virol 76\uff1a6004-6015\u3002\u3009View Article\u3000Google Scholar<\/p>\n

7.Si X, McManus BM, Zhang J, Yuan J, Cheung C, et al. (2005) Pyrrolidine Dithiocarbamate Reduces Coxsackievirus B3 Replication through Inhibition of the Ubiquitin-Proteasome Pathway. J Virol 79: 8014\u20138023.\u00a0\u00a0\u00a0 \u30087. Si X, McManus BM, Zhang J, Yuan J, Cheung C, et al. (2005) Pyrrolidine Dithiocarbamate \u901a\u904e\u6291\u5236\u6cdb\u7d20-\u86cb\u767d\u9176\u9ad4\u9014\u5f91\u6e1b\u5c11\u67ef\u85a9\u5947\u75c5\u6bd2 B3 \u8907\u88fd\u3002 J Virol 79\uff1a8014-8023\u3002\u3009View Article\u3000Google Scholar<\/p>\n

8.Korant BD, Kauer JC, Butterworth BE (1974) Zinc ions inhibit replication of rhinoviruses. Nature 248: 588\u2013590.\u00a0\u00a0\u00a0 \u30088. Korant BD, Kauer JC, Butterworth BE (1974) \u92c5\u96e2\u5b50\u6291\u5236\u9f3b\u75c5\u6bd2\u7684\u8907\u5236\u3002\u81ea\u7136 248\uff1a588-590\u3002\u3009View Article\u3000Google Scholar<\/p>\n

9.Polatnick J, Bachrach HL (1978) Effect of zinc and other chemical agents on foot-and-mouth-disease virus replication. Antimicrob Agents Chemother 13: 731\u2013734.\u00a0\u00a0 \u00a0\u30089. Polatnick J, Bachrach HL (1978) \u92c5\u548c\u5176\u4ed6\u5316\u5b78\u8a66\u5291\u5c0d\u53e3\u8e44\u75ab\u75c5\u6bd2\u8907\u88fd\u7684\u5f71\u97ff\u3002\u6297\u5fae\u751f\u7269\u5291 Chemother 13\uff1a731-734\u3002\u3009View Article\u3000Google Scholar<\/p>\n

10.Lanke K, Krenn BM, Melchers WJG, Seipelt J, van Kuppeveld FJM (2007) PDTC inhibits picornavirus polyprotein processing and RNA replication by transporting zinc ions into cells. J Gen Virol 88: 1206\u20131217.\u00a0\u00a0\u00a0 \u300810. Lanke K\u3001Krenn BM\u3001Melchers WJG\u3001Seipelt J\u3001van Kuppeveld FJM (2007) PDTC \u901a\u904e\u5c07\u92c5\u96e2\u5b50\u8f49\u904b\u5230\u7d30\u80de\u4e2d\u4f86\u6291\u5236\u5c0f\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u591a\u86cb\u767d\u52a0\u5de5\u548c RNA \u8907\u88fd\u3002 J Gen Virol 88\uff1a1206-1217\u3002\u3009View Article\u3000Google Scholar7<\/p>\n

11.Krenn BM, Gaudernak E, Holzer B, Lanke K, Van Kuppeveld FJM, et al. (2009) Antiviral Activity of the Zinc Ionophores Pyrithione and Hinokitiol against Picornavirus Infections. J Virol 83: 58\u201364.\u00a0\u00a0\u00a0 \u300811. Krenn BM\u3001Gaudernak E\u3001Holzer B\u3001Lanke K\u3001Van Kuppeveld FJM \u7b49\u3002 (2009) \u92c5\u96e2\u5b50\u8f09\u9ad4\u5421\u5576\u786b\u916e\u548c\u6a9c\u9187\u5c0d\u5c0f\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u611f\u67d3\u7684\u6297\u75c5\u6bd2\u6d3b\u6027\u3002 J Virol 83\uff1a58-64\u3002\u3009View Article\u3000Google Scholar<\/p>\n

12.Zalewski PD, Forbes IJ, Betts WH (1993) Correlation of apoptosis with change in intracellular labile Zn(II) using Zinquin [(2-methyl-8-p-toluenesulphonamide-6-quinolyloxy)acetic acid], a new specific fluorescent probe for Zn(II). Biochem J 296: 403\u2013408.\u00a0 \u300812. Zalewski PD, Forbes IJ, Betts WH (1993) \u4f7f\u7528 Zinquin [\uff082-\u7532\u57fa-8-\u5c0d\u7532\u82ef\u78fa\u9170\u80fa-6-\u55b9\u5549\u6c27\u57fa\uff09\u4e59\u9178]\uff0c\u7d30\u80de\u51cb\u4ea1\u8207\u7d30\u80de\u5167\u4e0d\u7a69\u5b9a\u92c5\uff08II\uff09\u8b8a\u5316\u7684\u76f8\u95dc\u6027\uff0c\u4e00\u7a2e\u65b0\u7684\u7279\u7570\u6027Zn(II) \u7684\u7192\u5149\u63a2\u91dd\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 296\uff1a403-408\u3002\u3009View Article\u3000Google Scholar<\/p>\n

13.Baum EZ, Bebernitz GA, Palant O, Mueller T, Plotch SJ (1991) Purification, properties, and mutagenesis of poliovirus 3C protease. Virology 165: 140\u2013150.\u00a0\u00a0\u00a0 \u300813. Baum EZ\u3001Bebernitz GA\u3001Palant O\u3001Mueller T\u3001Plotch SJ (1991) \u810a\u9ad3\u7070\u8cea\u708e\u75c5\u6bd2 3C \u86cb\u767d\u9176\u7684\u7d14\u5316\u3001\u7279\u6027\u548c\u8a98\u8b8a\u3002\u75c5\u6bd2\u5b78 165\uff1a140-150\u3002\u3009View Article\u3000Google Scholar<\/p>\n

14.Cordingley MG, Register RB, Callahan PL, Garsky VM, Colonno RJ (1989) Cleavage of small peptides in vitro by human rhinovirus 14 3C protease expressed in Escherichia coli. J Virol 63: 5037\u20135045.\u00a0\u00a0\u00a0 \u300814. Cordingley MG\u3001Register RB\u3001Callahan PL\u3001Garsky VM\u3001Colonno RJ (1989) \u5728\u5927\u8178\u687f\u83cc\u4e2d\u8868\u9054\u7684\u4eba\u9f3b\u75c5\u6bd2 14 3C \u86cb\u767d\u9176\u9ad4\u5916\u88c2\u89e3\u5c0f\u80bd\u3002 J Virol 63\uff1a5037-5045\u3002\u3009View Article\u3000Google Scholar<\/p>\n

15.Ferrari E, Wright-Minogue J, Fang JW, Baroudy BM, Lau JY, et al. (1999) Characterization of soluble hepatitis C virus RNA-dependent RNA polymerase expressed in Escherichia coli. J Virol 73: 1649\u20131654.\u00a0\u00a0\u00a0 \u300815. Ferrari E\u3001Wright-Minogue J\u3001Fang JW\u3001Baroudy BM\u3001Lau JY \u7b49\u3002 (1999) \u5728\u5927\u8178\u687f\u83cc\u4e2d\u8868\u9054\u7684\u53ef\u6eb6\u6027\u4e19\u578b\u809d\u708e\u75c5\u6bd2 RNA \u4f9d\u8cf4\u6027 RNA \u805a\u5408\u9176\u7684\u8868\u5fb5\u3002 J Virol 73\uff1a1649-1654\u3002\u3009View Article\u3000Google Scholar<\/p>\n

16.Hung M, Gibbs CS, Tsiang M (2002) Biochemical characterization of rhinovirus RNA-dependent RNA polymerase. Antiviral Res 56: 99\u2013114.\u00a0\u00a0\u00a0 \u300816. Hung M\u3001Gibbs CS\u3001Tsiang M (2002) \u9f3b\u75c5\u6bd2 RNA \u4f9d\u8cf4\u6027 RNA \u805a\u5408\u9176\u7684\u751f\u5316\u7279\u5fb5\u3002\u6297\u75c5\u6bd2\u7814\u7a76 56\uff1a99-114\u3002\u3009View Article\u3000Google Scholar<\/p>\n

17.Perlman S, Netland J (2009) Coronaviruses post-SARS: update on replication and pathogenesis. Nat Rev Micro 7: 439\u2013450.\u00a0\u00a0\u00a0 \u300817. Perlman S, Netland J (2009) SARS \u5f8c\u7684\u51a0\u72c0\u75c5\u6bd2\uff1a\u8907\u88fd\u548c\u767c\u75c5\u6a5f\u5236\u7684\u66f4\u65b0\u3002 Nat Rev Micro 7\uff1a439-450\u3002\u3009View Article\u3000Google Scholar<\/p>\n

18.Gorbalenya AE, Enjuanes L, Ziebuhr J, Snijder EJ (2006) Nidovirales: evolving the largest RNA virus genome. Virus Res 117: 17\u201337.\u00a0\u00a0\u00a0 \u300818. Gorbalenya AE\u3001Enjuanes L\u3001Ziebuhr J\u3001Snijder EJ (2006) Nidovirales\uff1a\u9032\u5316\u6700\u5927\u7684 RNA \u75c5\u6bd2\u57fa\u56e0\u7d44\u3002\u75c5\u6bd2\u7814\u7a76 117\uff1a17-37\u3002\u3009View Article\u3000Google Scholar<\/p>\n

19.Snijder EJ, Bredenbeek PJ, Dobbe JC, Thiel V, Ziebuhr J, et al. (2003) Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage. J Mol Biol 331: 991\u20131004.\u00a0\u00a0\u00a0 \u300819. Snijder EJ\u3001Bredenbeek PJ\u3001Dobbe JC\u3001Thiel V\u3001Ziebuhr J \u7b49\u3002 (2003) SARS \u51a0\u72c0\u75c5\u6bd2\u57fa\u56e0\u7d44\u548c\u86cb\u767d\u8cea\u7d44\u7684\u7368\u7279\u548c\u4fdd\u5b88\u7279\u5fb5\uff0c\u5f9e\u51a0\u72c0\u75c5\u6bd2\u7d44 2 \u8b5c\u7cfb\u7684\u65e9\u671f\u5206\u88c2\u3002 J Mol Biol 331\uff1a991-1004\u3002\u3009View Article\u3000Google Scholar<\/p>\n

20.Pasternak AO, Spaan WJ, Snijder EJ (2006) Nidovirus transcription: how to make sense\u2026? J Gen Virol 80: 1403\u20131421.\u00a0\u00a0\u00a0 \u300820. Pasternak AO\u3001Spaan WJ\u3001Snijder EJ (2006) Nidovirus \u8f49\u9304\uff1a\u5982\u4f55\u7406\u89e3\u2026\u2026\uff1f J Gen Virol 80\uff1a1403\u20131421\u3002\u3009View Article\u3000Google Scholar<\/p>\n

21.Sawicki SG, Sawicki DL, Siddell SG (2007) A Contemporary View of Coronavirus Transcription. J Virol 81: 20\u201329.\u00a0\u00a0\u00a0 \u300821. Sawicki SG\u3001Sawicki DL\u3001Siddell SG\uff082007\uff09\u51a0\u72c0\u75c5\u6bd2\u8f49\u9304\u7684\u7576\u4ee3\u89c0\u9ede\u3002 J Virol 81\uff1a20-29\u3002\u3009View Article\u3000Google Scholar<\/p>\n

22.Butterworth BE, Korant BD (1974) Characterization of the large picornaviral polypeptides produced in the presence of zinc ion. J Virol 14: 282\u2013291.\u00a0\u00a0\u00a0 \u300822. Butterworth BE, Korant BD (1974) \u5728\u92c5\u96e2\u5b50\u5b58\u5728\u4e0b\u7522\u751f\u7684\u5927\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u591a\u80bd\u7684\u8868\u5fb5\u3002 J Virol 14\uff1a282-291\u3002\u3009View Article\u3000Google Scholar<\/p>\n

23.Denison MR, Perlman S (1986) Translation and processing of mouse hepatitis virus virion RNA in a cell-free system. J Virol 60: 12\u201318.\u00a0\u00a0\u00a0 \u300823. Denison MR, Perlman S (1986) \u5728\u7121\u7d30\u80de\u7cfb\u7d71\u4e2d\u7ffb\u8b6f\u548c\u8655\u7406\u5c0f\u9f20\u809d\u708e\u75c5\u6bd2\u75c5\u6bd2\u9ad4 RNA\u3002 J Virol 60\uff1a12-18\u3002\u3009View Article\u3000Google Scholar<\/p>\n

24.Denison MR, Zoltick PW, Hughes SA, Giangreco B, Olson AL, et al. (1992) Intracellular processing of the N-terminal ORF 1a proteins of the coronavirus MHV-A59 requires multiple proteolytic events. Virology 189: 274\u2013284.\u00a0\u00a0\u00a0 \u300824. Denison MR\u3001Zoltick PW\u3001Hughes SA\u3001Giangreco B\u3001Olson AL \u7b49\u3002 (1992) \u51a0\u72c0\u75c5\u6bd2 MHV-A59 \u7684 N \u7aef ORF 1a \u86cb\u767d\u7684\u7d30\u80de\u5167\u52a0\u5de5\u9700\u8981\u591a\u500b\u86cb\u767d\u6c34\u89e3\u4e8b\u4ef6\u3002\u75c5\u6bd2\u5b78 189\uff1a274-284\u3002\u3009View Article\u3000Google Scholar<\/p>\n

25.van Hemert MJ, de Wilde AH, Gorbalenya AE, Snijder EJ (2008) The in Vitro RNA Synthesizing Activity of the Isolated Arterivirus Replication\/Transcription Complex Is Dependent on a Host Factor. J Biol Chem 283: 16525\u201316536.\u00a0\u00a0\u00a0 \u300825. van Hemert MJ\u3001de Wilde AH\u3001Gorbalenya AE\u3001Snijder EJ (2008) \u5206\u96e2\u7684\u52d5\u8108\u75c5\u6bd2\u8907\u88fd\/\u8f49\u9304\u8907\u5408\u7269\u7684\u9ad4\u5916 RNA \u5408\u6210\u6d3b\u6027\u53d6\u6c7a\u65bc\u5bbf\u4e3b\u56e0\u5b50\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 283\uff1a16525-16536\u3002\u3009View Article\u3000Google Scholar<\/p>\n

26.van Hemert MJ, van den Worm SHE, Knoops K, Mommaas AM, Gorbalenya AE, et al. (2008) SARS-Coronavirus Replication\/Transcription Complexes Are Membrane-Protected and Need a Host Factor for Activity In Vitro. PLoS Pathog 4: e1000054.\u00a0\u00a0\u00a0 \u300826. van Hemert MJ\u3001van den Worm SHE\u3001Knoops K\u3001Mommaas AM\u3001Gorbalenya AE \u7b49\u3002 (2008) SARS-\u51a0\u72c0\u75c5\u6bd2\u8907\u88fd\/\u8f49\u9304\u8907\u5408\u7269\u53d7\u819c\u4fdd\u8b77\uff0c\u9700\u8981\u5bbf\u4e3b\u56e0\u5b50\u624d\u80fd\u9032\u884c\u9ad4\u5916\u6d3b\u52d5\u3002 PLoS \u75c5\u539f\u9ad4 4\uff1ae1000054\u3002\u3009View Article\u3000Google Scholar<\/p>\n

27.te Velthuis AJ, Arnold JJ, Cameron CE, van den Worm SH, Snijder EJ (2009) The RNA polymerase activity of SARS-coronavirus nsp12 is primer dependent. Nucleic Acids Res 38: 203\u2013214.\u00a0\u00a0\u00a0 \u300827. te Velthuis AJ\u3001Arnold JJ\u3001Cameron CE\u3001van den Worm SH\u3001Snijder EJ (2009) SARS \u51a0\u72c0\u75c5\u6bd2 nsp12 \u7684 RNA \u805a\u5408\u9176\u6d3b\u6027\u4f9d\u8cf4\u65bc\u5f15\u7269\u3002\u6838\u9178\u7814\u7a76 38\uff1a203-214\u3002\u3009View Article\u3000Google Scholar<\/p>\n

28.Beerens N, Selisko B, Ricagno S, Imbert I, van der Zanden L, et al. (2007) De Novo Initiation of RNA Synthesis by the Arterivirus RNA-Dependent RNA Polymerase. J Virol 81: 8384\u20138395.\u00a0\u00a0\u00a0 \u300828. Beerens N\u3001Selisko B\u3001Ricagno S\u3001Imbert I\u3001van der Zanden L \u7b49\u3002 (2007) \u901a\u904e\u52d5\u8108\u75c5\u6bd2 RNA \u4f9d\u8cf4\u7684 RNA \u805a\u5408\u9176\u5f9e\u982d\u958b\u59cb\u5408\u6210 RNA\u3002 J Virol 81\uff1a8384\u20138395\u3002\u3009View Article\u3000Google Scholar<\/p>\n

29.van den Born E, Posthuma CC, Knoops K, Snijder EJ (2007) An infectious recombinant equine arteritis virus expressing green fluorescent protein from its replicase gene. J Gen Virol 88: 1196\u20131205.\u00a0\u00a0\u00a0 \u300829. van den Born E, Posthuma CC, Knoops K, Snijder EJ (2007) \u4e00\u7a2e\u50b3\u67d3\u6027\u91cd\u7d44\u99ac\u52d5\u8108\u708e\u75c5\u6bd2\uff0c\u5f9e\u5176\u8907\u5236\u9176\u57fa\u56e0\u8868\u9054\u7da0\u8272\u7192\u5149\u86cb\u767d\u3002 J Gen Virol 88\uff1a1196-1205\u3002\u3009View Article\u3000Google Scholar<\/p>\n

30.Sims AC, Burkett SE, Yount B, Pickles RJ (2008) SARS-CoV replication and pathogenesis in an in vitro model of the human conducting airway epithelium. Virus Res 133: 33\u201344.\u00a0\u00a0\u00a0 \u300830. Sims AC\u3001Burkett SE\u3001Yount B\u3001Pickles RJ (2008) SARS-CoV \u5728\u4eba\u985e\u50b3\u5c0e\u6c23\u9053\u4e0a\u76ae\u9ad4\u5916\u6a21\u578b\u4e2d\u7684\u8907\u5236\u548c\u767c\u75c5\u6a5f\u5236\u3002\u75c5\u6bd2\u7814\u7a76 133\uff1a33-44\u3002\u3009View Article\u3000Google Scholar<\/p>\n

31.Xu X, Liu Y, Weiss S, Arnold E, Sarafianos SG, et al. (2003) Molecular model of SARS coronavirus polymerase: implications for biochemical functions and drug design. Nucleic Acids Res 31: 7117\u20137130.\u00a0\u00a0\u00a0 \u300831. Xu X\u3001Liu Y\u3001Weiss S\u3001Arnold E\u3001Sarafianos SG \u7b49\u3002 (2003) SARS \u51a0\u72c0\u75c5\u6bd2\u805a\u5408\u9176\u7684\u5206\u5b50\u6a21\u578b\uff1a\u5c0d\u751f\u5316\u529f\u80fd\u548c\u85e5\u7269\u8a2d\u8a08\u7684\u5f71\u97ff\u3002\u6838\u9178\u7814\u7a76 31\uff1a7117-7130\u3002\u3009View Article\u3000Google Scholar<\/p>\n

32.Zhai Y, Sun F, Li X, Pang H, Xu X, et al. (2005) Insights into SARS-CoV transcription and replication from the structure of the nsp7-nsp8 hexadecamer. Nat Struct Mol Biol 12: 980\u2013986.\u00a0\u00a0\u00a0 \u300832. \u7fdf\u6bc5\u3001\u5b6b\u98db\u3001\u674e\u65ed\u3001\u9f90\u83ef\u3001\u5f90\u65ed\u7b49\u3002 (2005) \u5f9e nsp7-nsp8 \u5341\u516d\u805a\u9ad4\u7684\u7d50\u69cb\u6d1e\u5bdf SARS-CoV \u8f49\u9304\u548c\u5fa9\u5236\u3002 Nat Struct Mol Biol 12\uff1a980-986\u3002\u3009View Article\u3000Google Scholar<\/p>\n

33.Stockman LJ, Bellamy R, Garner P (2006) SARS: Systematic Review of Treatment Effects. PLoS Med 3: e343.\u00a0\u00a0\u00a0 \u300833. Stockman LJ\u3001Bellamy R\u3001Garner P (2006) SARS\uff1a\u6cbb\u7642\u6548\u679c\u7684\u7cfb\u7d71\u8a55\u50f9\u3002 PLoS Med 3: e343\u3002\u3009View Article\u3000Google Scholar<\/p>\n

34.Thompson A, Patel K, Tillman H, McHutchison JG (2009) Directly acting antivirals for the treatment of patients with hepatitis C infection: A clinical development update addressing key future challenges. J Hepatol 50: 184\u2013194.\u00a0\u00a0\u00a0 \u300834. Thompson A\u3001Patel K\u3001Tillman H\u3001McHutchison JG (2009) \u7528\u65bc\u6cbb\u7642\u4e19\u578b\u809d\u708e\u611f\u67d3\u60a3\u8005\u7684\u76f4\u63a5\u6297\u75c5\u6bd2\u85e5\u7269\uff1a\u61c9\u5c0d\u672a\u4f86\u95dc\u9375\u6311\u6230\u7684\u81e8\u5e8a\u958b\u767c\u66f4\u65b0\u3002 J Hepatol 50\uff1a184-194\u3002\u3009View Article\u3000Google Scholar<\/p>\n

35.De Clercq E (2004) Antivirals and antiviral strategies. Nat Rev Microbiol 2: 704\u2013720.\u00a0\u00a0\u00a0 \u300835. De Clercq E (2004) \u6297\u75c5\u6bd2\u85e5\u7269\u548c\u6297\u75c5\u6bd2\u7b56\u7565\u3002 Nat Rev Microbiol 2: 704\u2013720\u3002\u3009View Article\u3000Google Scholar<\/p>\n

36.Thompson AJV, McHutchison JG (2009) Antiviral resistance and specifically targeted therapy for HCV (STAT-C). J Viral Hepat 16: 377\u2013387.\u00a0\u00a0\u00a0 \u300836. Thompson AJV, McHutchison JG (2009) HCV \u6297\u75c5\u6bd2\u8010\u85e5\u6027\u548c\u7279\u7570\u6027\u9776\u5411\u6cbb\u7642 (STAT-C)\u3002 J \u75c5\u6bd2\u6027\u809d\u75c5 16\uff1a377-387\u3002\u3009View Article\u3000Google Scholar<\/p>\n

37.Yang W, Lee JY, Nowotny M (2006) Making and Breaking Nucleic Acids: Two-Mg2+-Ion Catalysis and Substrate Specificity. Mol Cell 22: 5\u201313.\u00a0\u00a0\u00a0 \u300837. Yang W\u3001Lee JY\u3001Nowotny M (2006) \u88fd\u9020\u548c\u7834\u58de\u6838\u9178\uff1a\u96d9 Mg2+ \u96e2\u5b50\u50ac\u5316\u548c\u5e95\u7269\u7279\u7570\u6027\u3002\u6469\u723e\u7d30\u80de 22\uff1a5-13\u3002\u3009View Article\u3000Google Scholar<\/p>\n

38.Castro C, Smidansky E, Maksimchuk KR, Arnold JJ, Korneeva VS, et al. (2007) Two proton transfers in the transition state for nucleotidyl transfer catalyzed by RNA- and DNA-dependent RNA and DNA polymerases. Proc Natl Acad Sci USA 104: 4267\u20134272.\u00a0\u00a0\u00a0 \u300838. Castro C\u3001Smidansky E\u3001Maksimchuk KR\u3001Arnold JJ\u3001Korneeva VS \u7b49\u3002 (2007) \u7531 RNA \u548c DNA \u4f9d\u8cf4\u6027 RNA \u548c DNA \u805a\u5408\u9176\u50ac\u5316\u7684\u6838\u82f7\u9178\u8f49\u79fb\u7684\u5169\u500b\u904e\u6e21\u614b\u8cea\u5b50\u8f49\u79fb\u3002 Proc Natl Acad Sci USA 104\uff1a4267\u20134272\u3002\u3009View Article\u3000Google Scholar<\/p>\n

39.Arnold JJ, Ghosh SK, Cameron CE (1999) Poliovirus RNA-dependent RNA Polymerase (3Dpol). Divalent cation modulation of primer, template and nucleotide selection. J Biol Chem 274: 37060\u201337069.\u00a0\u00a0\u00a0 \u300839. Arnold JJ\u3001Ghosh SK\u3001Cameron CE (1999) \u810a\u9ad3\u7070\u8cea\u708e\u75c5\u6bd2 RNA \u4f9d\u8cf4\u6027 RNA \u805a\u5408\u9176 (3Dpol)\u3002\u5f15\u7269\u3001\u6a21\u677f\u548c\u6838\u82f7\u9178\u9078\u64c7\u7684\u4e8c\u50f9\u967d\u96e2\u5b50\u8abf\u7bc0\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 274\uff1a37060-37069\u3002\u3009View Article\u3000Google Scholar<\/p>\n

40.Iuchi S (2001) Three classes of C2H2 zinc finger proteins. Cell Mol Life Sci 58: 625\u2013635.\u00a0\u00a0\u00a0 \u300840. Iuchi S (2001) \u4e09\u985e C2H2 \u92c5\u6307\u86cb\u767d\u3002\u7d30\u80de\u5206\u5b50\u751f\u547d\u79d1\u5b78 58\uff1a625-635\u3002\u3009View Article\u3000Google Scholar<\/p>\n

41.Gomis-Ruth XF (2009) Catalytic domain architecture of metzincin metalloproteases. J Biol Chem 284: 15353\u201315357.\u00a0\u00a0\u00a0 \u300841. Gomis-Ruth XF (2009) Metzincin \u91d1\u5c6c\u86cb\u767d\u9176\u7684\u50ac\u5316\u57df\u7d50\u69cb\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 284\uff1a15353\u201315357\u3002\u3009View Article\u3000Google Scholar<\/p>\n

42.Yap TL, Xu T, Chen Y-L, Malet H, Egloff M-P, et al. (2007) Crystal Structure of the Dengue Virus RNA-Dependent RNA Polymerase Catalytic Domain at 1.85-Angstrom Resolution. J Virol 81: 4753\u20134765.\u00a0\u00a0\u00a0 \u300842. Yap TL\u3001Xu T\u3001Chen Y-L\u3001Malet H\u3001Egloff M-P \u7b49\u3002 (2007) \u5206\u8fa8\u7387\u70ba 1.85 \u57c3\u7684\u767b\u9769\u71b1\u75c5\u6bd2\u4f9d\u8cf4\u65bc RNA \u7684 RNA \u805a\u5408\u9176\u50ac\u5316\u7d50\u69cb\u57df\u7684\u6676\u9ad4\u7d50\u69cb\u3002 J Virol 81\uff1a4753\u20134765\u3002\u3009View Article\u3000Google Scholar<\/p>\n

43.Winek CL, Buehler EV (1966) Intravenous toxicity of zinc pyridinethione and several zinc salts. Toxicol Appl Pharmacol 9: 296\u2013273.\u00a0\u00a0\u00a0 \u300843. Winek CL, Buehler EV (1966) \u5421\u5576\u786b\u916e\u92c5\u548c\u5e7e\u7a2e\u92c5\u9e7d\u7684\u975c\u8108\u6bd2\u6027\u3002 Toxicol Appl Pharmacol 9\uff1a296-273\u3002\u3009View Article\u3000Google Scholar<\/p>\n

44.Snyder DR, de Jesus CP, Towfighi J, Jacoby RO, Wedig JH (1979) Neurological, microscopic and enzyme-histochemical assessment of zinc pyrithione toxicity. Food Cosmet Toxicol 17: 651\u2013660.\u00a0\u00a0\u00a0 \u300844. Snyder DR, de Jesus CP, Towfighi J, Jacoby RO, Wedig JH (1979) \u5421\u5576\u786b\u916e\u92c5\u6bd2\u6027\u7684\u795e\u7d93\u5b78\u3001\u986f\u5fae\u93e1\u548c\u9176\u7d44\u7e54\u5316\u5b78\u8a55\u4f30\u3002\u98df\u54c1\u5316\u599d\u54c1\u6bd2\u7406\u5b78 17\uff1a651-660\u3002\u3009View Article\u3000Google Scholar<\/p>\n

45.Magda D, Lecane P, Wang Z, Hu W, Thiemann P, et al. (2008) Synthesis and anticancer properties of water-soluble zinc ionophores. Cancer Res 68: 5318\u20135325.\u00a0\u00a0\u00a0 \u300845. Magda D\u3001Lecane P\u3001Wang Z\u3001Hu W\u3001Thiemann P \u7b49\u3002 (2008) \u6c34\u6eb6\u6027\u92c5\u96e2\u5b50\u8f09\u9ad4\u7684\u5408\u6210\u548c\u6297\u764c\u7279\u6027\u3002\u764c\u75c7\u7814\u7a76 68\uff1a5318-5325\u3002\u3009View Article\u3000oogle Scholar<\/p>\n

46.Snijder EJ, van der Meer Y, Zevenhoven-Dobbe J, Onderwater JJM, van der Meulen J, et al. (2006) Ultrastructure and Origin of Membrane Vesicles Associated with the Severe Acute Respiratory Syndrome Coronavirus Replication Complex. J Virol 80: 5927\u20135940.\u00a0\u00a0\u00a0 \u300846. Snijder EJ\u3001van der Meer Y\u3001Zevenhoven-Dobbe J\u3001Onderwater JJM\u3001van der Meulen J \u7b49\u3002 (2006) \u8207\u56b4\u91cd\u6025\u6027\u547c\u5438\u7cfb\u7d71\u7d9c\u5408\u75c7\u51a0\u72c0\u75c5\u6bd2\u8907\u88fd\u8907\u5408\u9ad4\u76f8\u95dc\u7684\u819c\u56ca\u6ce1\u7684\u8d85\u5fae\u7d50\u69cb\u548c\u8d77\u6e90\u3002 J Virol 80\uff1a5927-5940\u3002\u3009View Article\u3000Google Scholar<\/p>\n

47.Studier F (2005) Protein production by auto-induction in high density shaking cultures. Protein Expr Purif 41: 207\u2013234.\u00a0\u00a0\u00a0 \u300847. Studier F (2005) \u5728\u9ad8\u5bc6\u5ea6\u632f\u76ea\u57f9\u990a\u4e2d\u901a\u904e\u81ea\u52d5\u8a98\u5c0e\u7522\u751f\u86cb\u767d\u8cea\u3002\u86cb\u767d\u8cea Expr Purif 41\uff1a207-234\u3002\u3009View Article\u3000Google Scholar<\/p>\n

 <\/p>\n

\u91cd\u5fb7\u4e26\u4e14\u5c07\u5728\u5317\u4eac\u7684\u300c\u4e2d\u570b\u79d1\u5b78\u9662\u751f\u7269\u7269\u7406\u7814\u7a76\u6240\u611f\u67d3\u8207\u514d\u75ab\u5b78\u91cd\u9ede\u5be6\u9a57\u5ba4\u300d\u5728\u91ab\u5b78\u96dc\u8a8c\u4e0a\u6240\u767c\u8868\u7684\u4e00\u7bc7\u82f1\u6587\u7814\u7a76\u5831\u544a\u300a\u92c5\u6307\u6297\u75c5\u6bd2\u86cb\u767d\u6291\u5236\u7532\u578b\u6d41\u611f\u75c5\u6bd2\u86cb\u767d\u8868\u9054\u4ee5\u53ca\u88ab\u75c5\u6bd2\u7de8\u78bcNS1 \u5c0d\u6297\u7684\u7c21\u8981\u5f62\u5f0f\u300b\u7684\u4e2d\u6587\u81ea\u8b6f\u7248\u4e5f\u5bc4\u4e0a\u7d66\u4ed6\u53c3\u8003(\u5982\u9644\u6a94)\u3002<\/p>\n

\u4e26\u4e14\uff0c\u91cd\u5fb7\u9084\u767c\u53bb\u4ee5\u4e0b\u5716\u7247\u8207\u8aaa\u660e\uff1a<\/p>\n

\"\"<\/p>\n

 <\/p>\n

\u300e\u9019\u5c31\u662f\u92c5\u6307\u7d50\u69cb\u5716\uff0c\u7531\u65bc\u9019\u662f\u87af\u5408\u7d50\u69cb\uff0c\u56e0\u6b64\u5b83\u80fd\u5f88\u5bb9\u6613\u5c07\u92c5\u96e2\u5b50\u7a7f\u900f\u7d30\u80de\u58c1\u800c\u8207\u8eb2\u85cf\u5728\u7d30\u80de\u5167\u840c\u767c\u7684\u75c5\u6bd2\u56e0\u70ba\u767c\u751f\u6291\u5236\u7ffb\u8b6f\u548c\u4fc3\u9032\u75c5\u6bd2 mRNA\u7684\u964d\u89e3\uff0c\u56e0\u6b64\u901a\u904e PARP \u7d50\u69cb\u57df\u800c\u4fc3\u9032 IAV \u86cb\u767d\u964d\u89e3\uff0c\u5be6\u9a57\u5df2\u7d93\u8b49\u5be6\u5b83\u53ef\u8b93\u5165\u4fb5\u5230\u7d30\u80de\u5167\u7684\u75c5\u6bd2\u4e4bRNA\u6a5f\u8f49\u767c\u751f\u4f11\u514b(\u4f46\u4e0d\u4e00\u5b9a\u6703\u6b7b\u4ea1)\uff0c\u800c\u4f7f\u5f97\u539f\u672c\u7121\u751f\u547d\u7684\u75c5\u6bd2\u56e0\u6b64\u4e0d\u80fd\u5927\u91cf\u8907\u88fd\u840c\u751f\uff0c\u800c\u53ef\u4ee5\u70ba\u4eba\u9ad4\u7684\u514d\u75ab\u7cfb\u7d71\u89e3\u6c7a\u3002\u300f<\/p>\n

\u9019\u4e00\u4f4d\u738b\u59d3\u7db2\u53cb\u53c8\u767c\u4f86\u4ee5\u4e0b\u4fe1\u606f\uff1a\u300e\u80fd\u5920\u6bba\u75c5\u6bd2\u800c\u4e0d\u6703\u6bba\u6b7b\u6211\u5011\u81ea\u5df1\u7684\u7d30\u80de,\u5929\u4e0b\u6709\u9019\u9ebc\u597d\u7684\u4e8b\u55ce\uff1f\u4e0d\u904e\u662f\u71df\u990a\u88dc\u52a9\u54c1\u800c\u5df2\uff01\u300f<\/p>\n

\u91cd\u5fb7\u770b\u4ed6\u5728\u610f\u6c23\u7528\u4e8b\uff0c\u56e0\u6b64\u5c31\u56de\u8986\u4ed6\u8aaa\uff1a\u300e\u96a8\u60a8\u9ad8\u8208\u600e\u9ebc\u8aaa\u90fd\u597d\uff0c\u6211\u6c92\u6709\u8aaa\u5b83\u662f\u85e5\uff0c\u53ea\u8aaa\u662f\u4fdd\u5065\u54c1\u800c\u5df2\u3002\u300f\u767c\u5b8c\u4e86\u9019\u4fe1\u606f\u4e4b\u5f8c\uff0c\u91cd\u5fb7\u767c\u8a93\u4ee5\u5f8c\u518d\u4e5f\u4e0d\u5c0d\u9019\u7a2e\u96de\u86cb\u88e1\u6311\u9aa8\u982d\u7684\u4eba\u63d0\u4f9b\u4efb\u4f55\u6709\u50f9\u503c\u7684\u8cc7\u8a0a\u4e86\u3002<\/p>\n

\u4f46\u662f\uff0c\u91cd\u5fb7\u9084\u662f\u8981\u544a\u8a34\u5927\u5bb6\u70ba\u4f55\u8981\u5efa\u8b70\u5927\u5bb6\u5403\u542b\u92c5\u96e2\u5b50\u7684\u4fdd\u5065\u98df\u54c1\uff0c\u7576\u7136\u92c5\u5f9e\u98df\u7269\u4e2d\u651d\u53d6\u4e5f\u6709\uff0c\u4f46\u662f\u98df\u7269\u5fc5\u9808\u7d93\u904e\u6d88\u5316\u5f8c\u92c5\u96e2\u5b50\u624d\u6703\u900f\u904e\u8840\u6db2\u800c\u5230\u4eba\u9ad4\u5668\u5b98\uff0c\u9019\u500b\u904e\u7a0b\u592a\u9577\u592a\u4e45\u4e86\uff0c\u56e0\u6b64\u92c5\u96e2\u5b50\u53ef\u80fd\u53c8\u8207\u5176\u4ed6\u7269\u8cea\u7d50\u5408\u6210\u5316\u5408\u7269\uff0c\u800c\u518d\u4e0d\u80fd\u767c\u751f\u8207\u8ca0\u96e2\u5b50\u7684\u75c5\u6bd2\u4e2d\u548c\u7684\u6548\u679c\u3002\u56e0\u6b64\u5c31\u66f4\u6c92\u6709\u767c\u63ee\u5230\u92c5\u96e2\u5b50\u6240\u7368\u5177\u7684\u87af\u5408\u4ea4\u63db\u6548\u679c\u3002<\/p>\n

\u7531\u65bc\uff0c\u611f\u5192\u6216\u662fCOVID-19\u7684\u521d\u671f\u75c5\u72c0\u4e3b\u8981\u90fd\u662f\u91dd\u5c0d\u4e0a\u652f\u6c23\u7ba1\uff0c\u56e0\u6b64\u4ee3\u8868\u547c\u5438\u7ba1\u9053\u7684\u75c5\u6bd2\u91cf\u6700\u5927\uff0c\u56e0\u6b64\u53ef\u4ee5\u542b\u5728\u5634\u4e2d\u6162\u6162\u5206\u89e3\u7684\u5589\u7cd6\u4e4b\u6548\u679c\u6700\u597d\uff0c\u56e0\u70ba\u5b83\u8b93\u901a\u904e\u7684\u6c23\u6d41\u4e2d\u7684\u75c5\u6bd2\u5148\u88ab\u92c5\u96e2\u5b50\u4e2d\u548c\uff0c\u800c\u7cd6\u6c41\u6d41\u5165\u98df\u9053\u6642\u92c5\u96e2\u5b50\u4e5f\u6703\u6ea2\u5165\u4e0a\u652f\u6c23\u7ba1\u3002\u9019\u5c31\u662f\u70ba\u4f55\u91cd\u5fb7\u9996\u5148\u63a8\u85a6\u5df2\u7d93\u5728\u7f8e\u570b\u98a8\u884c30\u591a\u5e74\u7684\u611f\u5192\u9810\u9632\u7528\u85e5Cold Eeze\u4e2d\u6587\u8b6f\u540d\u70ba\u3010\u92c5\u7cd6\u3011\uff0c\u56e0\u70ba\u5b83\u5df2\u7d93\u904e\u4e86\u5c08\u5229\u4fdd\u8b77\u671f\uff0c\u5b78\u540d\u85e5\u540d\u662fGeneric Version\uff0c\u56e0\u70ba\u552e\u50f9\u8f03\u4fbf\u5b9c(\u4f46\u662f\u73fe\u5728\u5168\u7403\u6436\u8cfc\u5f88\u53ef\u80fd\u7f3a\u8ca8)\u3002<\/p>\n

\u73fe\u5728\uff0c\u91cd\u5fb7\u8981\u544a\u8a34\u5927\u5bb6\u4e00\u500b\u79d8\u5bc6\uff0c\u90a3\u5c31\u662f\u70ba\u4f55\u91cd\u5fb7\u8981\u5c0d\u92c5\u96e2\u5b50\u9032\u884c\u7814\u7a76\u5462\uff0c\u6545\u4e8b\u662f\u9019\u6a23\u5b50\u7684\uff0c\u7531\u65bc\u4e00\u5e74\u524d \u91cd\u5fb7 \u8acb\u793a\u201c\u9748\u7236\u201d(\u5143\u59cb\u5929\u5c0a)\u8aaa\uff1a\u300e\u65e2\u7136\u00a0 \u9748\u7236\u80fd\u63d0\u524d\u534a\u5e74\u8b66\u544a\u6211\u5011\u5c07\u6709\u6b64\u5927\u75ab\u60c5\uff0c\u662f\u5426\u80fd\u544a\u77e5\u5f92\u5152\u5982\u4f55\u88fd\u5f97\u89e3\u85e5\uff1f\u300f\u64f2\u832d\u61c9\u8a31\u5f8c\uff0c\u91cd\u5fb7 \u6b63\u4e0d\u77e5\u5982\u4f55\u518d\u554f\u9019\u89e3\u85e5\u79d8\u65b9\u6642\uff0c\u8166\u4e2d\u7a81\u7136\u6e67\u73fe\u4e00\u500b\u201c\u92c5\u201d\u5b57\uff0c\u64f2\u832d\u518d\u554f\u78ba\u5b9a\u795e\u8aed\u5c31\u662f\u6b64\u201c\u92c5\u201d\u5b57\u7121\u8aa4\u3002<\/p>\n

\u5728\u6b64\u4e4b\u5f8c\uff0c\u91cd\u5fb7\u624d\u958b\u59cb\u8a73\u95b1\u5404\u7a2e\u82f1\u6587\u91ab\u5b78\u6587\u737b\uff0c\u8d8a\u767c\u8b49\u5be6\u92c5\u96e2\u5b50\u6709\u6291\u5236mRNA\u75c5\u6bd2\u7684\u6548\u679c\uff0c\u800c\u4e14\u56e0\u70ba\u92c5\u96e2\u5b50\u662f\u63a1\u53d6\u87af\u5408\u65b9\u5f0f\u8b93\u92c5\u96e2\u5b50\u7a7f\u900f\u7d30\u80de\u58c1\u9032\u5165\u4f86\u964d\u89e3\u75c5\u6bd2\u86cb\u767d\u8907\u88fd\u6240\u9700\u6c42\u7684PARP \u7d50\u69cb\u57df\uff0c\u56e0\u6b64\u9019\u662f\u4e00\u7a2e\u7cbe\u6e96\u7834\u7a97\u5f0f\u7684\u65ac\u9996\u884c\u52d5\uff0c\u5c0d\u65bc\u7d30\u80de\u7684\u50b7\u5bb3\u751a\u5c0f\uff0c\u56e0\u6b64\u5c0d\u65bc\u4eba\u9ad4\u7684\u5371\u5bb3\u751a\u4f4e\u3002<\/p>\n

\u91cd\u5fb7\u4e00\u5e74\u524d\u5c31\u628a\u7814\u7a76\u6210\u679c\u516c\u958b\uff0c\u4e00\u4f86\u56e0\u70ba\u81ea\u5df1\u962e\u56ca\u7f9e\u6f80\uff0c\u7121\u6cd5\u7e7c\u7e8c\u7814\u767c\uff0c\u4e8c\u4f86\u91cd\u5fb7\u5df2\u7d93\u9810\u7aba\u5230\u672a\u4f86\u9084\u6709\u5176\u4ed6\u56b4\u91cd\u75be\u75c5\uff0c\u800c\u4e14COVID-19\u9084\u6703\u5927\u7a81\u8b8a\uff0c\u56e0\u6b64\u8b93\u5176\u4ed6\u4eba\u63a5\u68d2\u4f86\u7814\u7a76\u6216\u8a31\u6703\u66f4\u6709\u6210\u6548\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u25bc\u4e00\u822c\u4eba\u5f9e\u98df\u7269\u4e2d\u651d\u53d6\u201c\u92c5\u201d\u70ba\u7684\u5c31\u662f\u58ef\u967d\u8207\u589e\u9aee\u3002\uff08\u5716\uff0f\u674e\u91cd\u5fb7\u63d0\u4f9b\uff09<\/p>\n","protected":false},"author":1,"featured_media":104516,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"fifu_image_url":"","fifu_image_alt":"","footnotes":""},"categories":[11],"tags":[9360,34266,34267],"class_list":["post-104514","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-11","tag-9360","tag-34266","tag-34267"],"acf":[],"yoast_head":"\n\u597d\u201c\u92c5\u201d\u3000\u4e5f\u6703\u60f9\u4e86\u4e00\u8eab\u8165 - \u5c4f\u6771\u6642\u5831\u96fb\u5b50\u5831<\/title>\n<meta name=\"description\" content=\"\u3010\u674e\u91cd\u5fb7\uff0f\u5c08\u6b04\u5831\u5c0e2022\/05\/27\u3011\u3000\u4eca\u65e9\uff0c\u6709\u4e00\u78ba\u8a3a\u7652\u5f8c\u8005\u8207\u91cd\u5fb7\u8a0e\u8ad6\u4ed6\u7684\u75c5\u60c5\uff0c\u91cd\u5fb7\u5efa\u8b70\u4ed6\u88dc\u5145\u92c5\u96e2\u5b50\u98df\u54c1\uff0c\u4e26\u5c07\u6b64\u4e00\u4fe1\u606f\u4e5f\u767c\u7d66\u5927\u5bb6\u77e5\u66c9\uff1a \u300e\u6709\u78ba\u8a3a\u7652\u5f8c\u8005\u554f\u91cd\u5fb7\u8981\u5982\u4f55\u4fdd\u5065\u4ee5\u9632\u5fa9\u967d\uff0c\u91cd\u5fb7 \u7686\u5efa\u8b70\u4ed6\u5403\u3010\u92c5\u7cd6\u3011(\u985e\u4f3c\u5589\u7247)\u5c0f\u5b69\u5247\u5403\u3010\u5152\u7ae5\u92c5\u8edf\u7cd6\u3011\uff0c\u6216\u662f\u3010\u9175\u6bcd\u92c5\u3011\u3001\u3010\u87af\u5408\u92c5\u3011\u6216\u662f\u3010\u8461\u8404\u7cd6\u9178\u92c5\u3011\u4f86\u6d88\u6ec5\u6f5b\u85cf\u4eba\u9ad4\u5c1a\u672a\u767c\u4f5c\u7684\u75c5\u6bd2\u3002\u300f \u5f8c\u4f86\u5c31\u63a5\u7372\u4e00\u4f4d\u738b\u59d3\u7db2\u53cb\u56de\u8986\u8aaa\uff1a\u300e\u4e0d\u5c0d\u5594\uff01\u92c5,\u4eba\u985e\u5b58\u6d3b\u6240\u9700\u8981\u7684\u5143\u7d20\u4e4b\u4e00,\u5f9e\u5929\u7136\u98df\u7269\u76f4\u63a5\u651d\u53d6\u5c31\u8db3\u5920\u4e86,\u4eba\u70ba\u8d85\u91cf\u7684\u88dc\u5145\u6703\u51fa\u554f\u984c\u7684\uff01\u92c5,\u4e0d\u6703\u76f4\u63a5\u6bba\u75c5\u6bd2\u7684\uff01\u300f \u9019\u4e00\u4f4d\u738b\u5148\u751f\uff0c\u5927\u6982\u6f0f\u770b\u4e86\u91cd\u5fb7\u5728\u4e00\u5e74\u524d\u5728\u672c\u6703\u7fa4\u7d44\u4e0a\u767c\u8868\u7684\u5e7e\u7bc7\u4ee5\u92c5\u96e2\u5b50\u4f86\u6d88\u6ec5\u75c5\u6bd2\u7684\u6587\u7ae0\uff0c\u56e0\u6b64\uff0c\u91cd\u5fb7\u5c31\u56de\u8986\u4ed6\u8aaa\uff1a\u300e\u6211\u770b\u904e\u4e86\u8a31\u591a\u82f1\u6587\u91ab\u5b78\u6587\u737b\uff0c\u4e5f\u8207\u9ec3\u25cb\u5bcc\u535a\u58eb\u548c\u9673\u25cb\u5e73\u91ab\u5e2b\u8a0e\u8ad6\u904e\u3002\u60a8\u5982\u679c\u60f3\u8981\u770b\u9019\u4e00\u4e9b\u82f1\u6587\u91ab\u5b78\u6587\u737b\uff0c\u6211\u53ef\u4ee5\u5bc4\u53bb\u7d66\u60a8\u3002\u300f\u7136\u5f8c \u91cd\u5fb7 \u5c31\u5c31\u5bc4\u53bb\u7d66\u4ed6\u4ee5\u4e0b\u7684\u8cc7\u6599\uff1a References\u53c3\u8003\u6587\u737b 1.Lazarczyk M, Favre M (2008) Role of Zn2+ ions in host-virus interactions. J Virol 82: 11486\u201311494.\u00a0\u00a0\u00a0 \u30081. Lazarczyk M, Favre M (2008) Zn2+ \u96e2\u5b50\u5728\u5bbf\u4e3b-\u75c5\u6bd2\u76f8\u4e92\u4f5c\u7528\u4e2d\u7684\u4f5c\u7528\u3002 J Virol 82\uff1a11486\u201311494\u3002\u3009\u3000View Article\u3000Google Scholar 2.Frederickson CJ, Koh JY, Bush AI (2005) Neurobiology of zinc in health and disease. Nat Rev Neurosci 6: 449\u2013462.\u00a0\u00a0\u00a0 \u30082. Frederickson CJ\u3001Koh JY\u3001Bush AI (2005) \u92c5\u5728\u5065\u5eb7\u548c\u75be\u75c5\u4e2d\u7684\u795e\u7d93\u751f\u7269\u5b78\u3002 Nat Rev Neurosci 6\uff1a449-462\u3002\u3009View Article\u3000Google Scholar 3.Alirezaei M, Nairn AC, Glowinski J, Premont J, Marin P (1999) Zinc inhibits protein synthesis in neurons: potential rol of phosphorylation of translation initiation factor-2a. J Biol Chem 274: 32433\u201332438.\u00a0\u00a0\u00a0 \u30083. Alirezaei M\u3001Nairn AC\u3001Glowinski J\u3001Premont J\u3001Marin P (1999) \u92c5\u6291\u5236\u795e\u7d93\u5143\u4e2d\u7684\u86cb\u767d\u8cea\u5408\u6210\uff1a\u7ffb\u8b6f\u8d77\u59cb\u56e0\u5b50 2a \u78f7\u9178\u5316\u7684\u6f5b\u5728\u4f5c\u7528\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 274\uff1a32433\u201332438\u3002\u3009View Article\u3000Google Scholar 4.Uchide N, Ohyama K, Bessho T, Yuan B, Yamakawa T (2002) Effect of antioxidants on apoptosis induced by influenza virus infection: inhibition of viral gene replication and transcription with pyrrolidine dithiocarbamate. Antiviral Res 56: 207\u2013217.\u00a0\u00a0\u00a0 \u30084. Uchide N, Ohyama K, Bessho T, Yuan B, Yamakawa T (2002) \u6297\u6c27\u5316\u5291\u5c0d\u6d41\u611f\u75c5\u6bd2\u611f\u67d3\u8a98\u5c0e\u7684\u7d30\u80de\u51cb\u4ea1\u7684\u5f71\u97ff\uff1a\u7528\u5421\u54af\u70f7\u4e8c\u786b\u4ee3\u6c28\u57fa\u7532\u9178\u916f\u6291\u5236\u75c5\u6bd2\u57fa\u56e0\u8907\u5236\u548c\u8f49\u9304\u3002\u6297\u75c5\u6bd2\u7814\u7a76 56\uff1a207-217\u3002\u3009View Article\u3000Google Scholar 5.Suara RO, Crowe JEJ (2004) Effect of zinc salts on respiratory syncytial virus replication. Antimicrob Agents Chemother 48: 783\u2013790.\u00a0\u00a0\u00a0 \u30085. Suara RO, Crowe JEJ (2004) \u92c5\u9e7d\u5c0d\u547c\u5438\u9053\u5408\u80de\u75c5\u6bd2\u8907\u88fd\u7684\u5f71\u97ff\u3002\u6297\u5fae\u751f\u7269\u5291 Chemother 48\uff1a783-790\u3002\u3009View Article\u3000Google Scholar 6.Gaudernak E, Seipelt J, Triendl A, Grassauer A, Kuechler E (2002) Antiviral Effects of Pyrrolidine Dithiocarbamate on Human Rhinoviruses. J Virol 76: 6004\u20136015.\u00a0\u00a0\u00a0 \u30086. Gaudernak E\u3001Seipelt J\u3001Triendl A\u3001Grassauer A\u3001Kuechler E (2002) \u5421\u54af\u70f7\u4e8c\u786b\u4ee3\u6c28\u57fa\u7532\u9178\u916f\u5c0d\u4eba\u9f3b\u75c5\u6bd2\u7684\u6297\u75c5\u6bd2\u4f5c\u7528\u3002 J Virol 76\uff1a6004-6015\u3002\u3009View Article\u3000Google Scholar 7.Si X, McManus BM, Zhang J, Yuan J, Cheung C, et al. (2005) Pyrrolidine Dithiocarbamate Reduces Coxsackievirus B3 Replication through Inhibition of the Ubiquitin-Proteasome Pathway. J Virol 79: 8014\u20138023.\u00a0\u00a0\u00a0 \u30087. Si X, McManus BM, Zhang J, Yuan J, Cheung C, et al. (2005) Pyrrolidine Dithiocarbamate \u901a\u904e\u6291\u5236\u6cdb\u7d20-\u86cb\u767d\u9176\u9ad4\u9014\u5f91\u6e1b\u5c11\u67ef\u85a9\u5947\u75c5\u6bd2 B3 \u8907\u88fd\u3002 J Virol 79\uff1a8014-8023\u3002\u3009View Article\u3000Google Scholar 8.Korant BD, Kauer JC, Butterworth BE (1974) Zinc ions inhibit replication of rhinoviruses. Nature 248: 588\u2013590.\u00a0\u00a0\u00a0 \u30088. Korant BD, Kauer JC, Butterworth BE (1974) \u92c5\u96e2\u5b50\u6291\u5236\u9f3b\u75c5\u6bd2\u7684\u8907\u5236\u3002\u81ea\u7136 248\uff1a588-590\u3002\u3009View Article\u3000Google Scholar 9.Polatnick J, Bachrach HL (1978) Effect of zinc and other chemical agents on foot-and-mouth-disease virus replication. Antimicrob Agents Chemother 13: 731\u2013734.\u00a0\u00a0 \u00a0\u30089. Polatnick J, Bachrach HL (1978) \u92c5\u548c\u5176\u4ed6\u5316\u5b78\u8a66\u5291\u5c0d\u53e3\u8e44\u75ab\u75c5\u6bd2\u8907\u88fd\u7684\u5f71\u97ff\u3002\u6297\u5fae\u751f\u7269\u5291 Chemother 13\uff1a731-734\u3002\u3009View Article\u3000Google Scholar 10.Lanke K, Krenn BM, Melchers WJG, Seipelt J, van Kuppeveld FJM (2007) PDTC inhibits picornavirus polyprotein processing and RNA replication by transporting zinc ions into cells. J Gen Virol 88: 1206\u20131217.\u00a0\u00a0\u00a0 \u300810. Lanke K\u3001Krenn BM\u3001Melchers WJG\u3001Seipelt J\u3001van Kuppeveld FJM (2007) PDTC \u901a\u904e\u5c07\u92c5\u96e2\u5b50\u8f49\u904b\u5230\u7d30\u80de\u4e2d\u4f86\u6291\u5236\u5c0f\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u591a\u86cb\u767d\u52a0\u5de5\u548c RNA \u8907\u88fd\u3002 J Gen Virol 88\uff1a1206-1217\u3002\u3009View Article\u3000Google Scholar7 11.Krenn BM, Gaudernak E, Holzer B, Lanke K, Van Kuppeveld FJM, et al. (2009) Antiviral Activity of the Zinc Ionophores Pyrithione and Hinokitiol against Picornavirus Infections. J Virol 83: 58\u201364.\u00a0\u00a0\u00a0 \u300811. Krenn BM\u3001Gaudernak E\u3001Holzer B\u3001Lanke K\u3001Van Kuppeveld FJM \u7b49\u3002 (2009) \u92c5\u96e2\u5b50\u8f09\u9ad4\u5421\u5576\u786b\u916e\u548c\u6a9c\u9187\u5c0d\u5c0f\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u611f\u67d3\u7684\u6297\u75c5\u6bd2\u6d3b\u6027\u3002 J Virol 83\uff1a58-64\u3002\u3009View Article\u3000Google Scholar 12.Zalewski PD, Forbes IJ, Betts WH (1993) Correlation of apoptosis with change in intracellular labile Zn(II) using Zinquin , a new specific fluorescent probe for Zn(II). Biochem J 296: 403\u2013408.\u00a0 \u300812. Zalewski PD, Forbes IJ, Betts WH (1993) \u4f7f\u7528 Zinquin \uff0c\u7d30\u80de\u51cb\u4ea1\u8207\u7d30\u80de\u5167\u4e0d\u7a69\u5b9a\u92c5\uff08II\uff09\u8b8a\u5316\u7684\u76f8\u95dc\u6027\uff0c\u4e00\u7a2e\u65b0\u7684\u7279\u7570\u6027Zn(II) \u7684\u7192\u5149\u63a2\u91dd\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 296\uff1a403-408\u3002\u3009View Article\u3000Google Scholar 13.Baum EZ, Bebernitz GA, Palant O, Mueller T, Plotch SJ (1991) Purification, properties, and mutagenesis of poliovirus 3C protease. Virology 165: 140\u2013150.\u00a0\u00a0\u00a0 \u300813. Baum EZ\u3001Bebernitz GA\u3001Palant O\u3001Mueller T\u3001Plotch SJ (1991) \u810a\u9ad3\u7070\u8cea\u708e\u75c5\u6bd2 3C \u86cb\u767d\u9176\u7684\u7d14\u5316\u3001\u7279\u6027\u548c\u8a98\u8b8a\u3002\u75c5\u6bd2\u5b78 165\uff1a140-150\u3002\u3009View Article\u3000Google Scholar 14.Cordingley MG, Register RB, Callahan PL, Garsky VM, Colonno RJ (1989) Cleavage of small peptides in vitro by human rhinovirus 14 3C protease expressed in Escherichia coli. J Virol 63: 5037\u20135045.\u00a0\u00a0\u00a0 \u300814. Cordingley MG\u3001Register RB\u3001Callahan PL\u3001Garsky VM\u3001Colonno RJ (1989) \u5728\u5927\u8178\u687f\u83cc\u4e2d\u8868\u9054\u7684\u4eba\u9f3b\u75c5\u6bd2 14 3C \u86cb\u767d\u9176\u9ad4\u5916\u88c2\u89e3\u5c0f\u80bd\u3002 J Virol 63\uff1a5037-5045\u3002\u3009View Article\u3000Google Scholar 15.Ferrari E, Wright-Minogue J, Fang JW, Baroudy BM, Lau JY, et al. (1999) Characterization of soluble hepatitis C virus RNA-dependent RNA polymerase expressed in Escherichia coli. J Virol 73: 1649\u20131654.\u00a0\u00a0\u00a0 \u300815. Ferrari E\u3001Wright-Minogue J\u3001Fang JW\u3001Baroudy BM\u3001Lau JY \u7b49\u3002 (1999) \u5728\u5927\u8178\u687f\u83cc\u4e2d\u8868\u9054\u7684\u53ef\u6eb6\u6027\u4e19\u578b\u809d\u708e\u75c5\u6bd2 RNA \u4f9d\u8cf4\u6027 RNA \u805a\u5408\u9176\u7684\u8868\u5fb5\u3002 J Virol 73\uff1a1649-1654\u3002\u3009View Article\u3000Google Scholar 16.Hung M, Gibbs CS, Tsiang M (2002) Biochemical characterization of rhinovirus RNA-dependent RNA polymerase. Antiviral Res 56: 99\u2013114.\u00a0\u00a0\u00a0 \u300816. Hung M\u3001Gibbs CS\u3001Tsiang M (2002) \u9f3b\u75c5\u6bd2 RNA \u4f9d\u8cf4\u6027 RNA \u805a\u5408\u9176\u7684\u751f\u5316\u7279\u5fb5\u3002\u6297\u75c5\u6bd2\u7814\u7a76 56\uff1a99-114\u3002\u3009View Article\u3000Google Scholar 17.Perlman S, Netland J (2009) Coronaviruses post-SARS: update on replication and pathogenesis. Nat Rev Micro 7: 439\u2013450.\u00a0\u00a0\u00a0 \u300817. Perlman S, Netland J (2009) SARS \u5f8c\u7684\u51a0\u72c0\u75c5\u6bd2\uff1a\u8907\u88fd\u548c\u767c\u75c5\u6a5f\u5236\u7684\u66f4\u65b0\u3002 Nat Rev Micro 7\uff1a439-450\u3002\u3009View Article\u3000Google Scholar 18.Gorbalenya AE, Enjuanes L, Ziebuhr J, Snijder EJ (2006) Nidovirales: evolving the largest RNA virus genome. Virus Res 117: 17\u201337.\u00a0\u00a0\u00a0 \u300818. Gorbalenya AE\u3001Enjuanes L\u3001Ziebuhr J\u3001Snijder EJ (2006) Nidovirales\uff1a\u9032\u5316\u6700\u5927\u7684 RNA \u75c5\u6bd2\u57fa\u56e0\u7d44\u3002\u75c5\u6bd2\u7814\u7a76 117\uff1a17-37\u3002\u3009View Article\u3000Google Scholar 19.Snijder EJ, Bredenbeek PJ, Dobbe JC, Thiel V, Ziebuhr J, et al. (2003) Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage. J Mol Biol 331: 991\u20131004.\u00a0\u00a0\u00a0 \u300819. Snijder EJ\u3001Bredenbeek PJ\u3001Dobbe JC\u3001Thiel V\u3001Ziebuhr J \u7b49\u3002 (2003) SARS \u51a0\u72c0\u75c5\u6bd2\u57fa\u56e0\u7d44\u548c\u86cb\u767d\u8cea\u7d44\u7684\u7368\u7279\u548c\u4fdd\u5b88\u7279\u5fb5\uff0c\u5f9e\u51a0\u72c0\u75c5\u6bd2\u7d44 2 \u8b5c\u7cfb\u7684\u65e9\u671f\u5206\u88c2\u3002 J Mol Biol 331\uff1a991-1004\u3002\u3009View Article\u3000Google Scholar 20.Pasternak AO, Spaan WJ, Snijder EJ (2006) Nidovirus transcription: how to make sense\u2026? J Gen Virol 80: 1403\u20131421.\u00a0\u00a0\u00a0 \u300820. Pasternak AO\u3001Spaan WJ\u3001Snijder EJ (2006) Nidovirus \u8f49\u9304\uff1a\u5982\u4f55\u7406\u89e3\u2026\u2026\uff1f J Gen Virol 80\uff1a1403\u20131421\u3002\u3009View Article\u3000Google Scholar 21.Sawicki SG, Sawicki DL, Siddell SG (2007) A Contemporary View of Coronavirus Transcription. J Virol 81: 20\u201329.\u00a0\u00a0\u00a0 \u300821. Sawicki SG\u3001Sawicki DL\u3001Siddell SG\uff082007\uff09\u51a0\u72c0\u75c5\u6bd2\u8f49\u9304\u7684\u7576\u4ee3\u89c0\u9ede\u3002 J Virol 81\uff1a20-29\u3002\u3009View Article\u3000Google Scholar 22.Butterworth BE, Korant BD (1974) Characterization of the large picornaviral polypeptides produced in the presence of zinc ion. J Virol 14: 282\u2013291.\u00a0\u00a0\u00a0 \u300822. Butterworth BE, Korant BD (1974) \u5728\u92c5\u96e2\u5b50\u5b58\u5728\u4e0b\u7522\u751f\u7684\u5927\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u591a\u80bd\u7684\u8868\u5fb5\u3002 J Virol 14\uff1a282-291\u3002\u3009View Article\u3000Google Scholar 23.Denison MR, Perlman S (1986) Translation and processing of mouse hepatitis virus virion RNA in a cell-free system. J Virol 60: 12\u201318.\u00a0\u00a0\u00a0 \u300823. Denison MR, Perlman S (1986) \u5728\u7121\u7d30\u80de\u7cfb\u7d71\u4e2d\u7ffb\u8b6f\u548c\u8655\u7406\u5c0f\u9f20\u809d\u708e\u75c5\u6bd2\u75c5\u6bd2\u9ad4 RNA\u3002 J Virol 60\uff1a12-18\u3002\u3009View Article\u3000Google Scholar 24.Denison MR, Zoltick PW, Hughes SA, Giangreco B, Olson AL, et al. (1992) Intracellular processing of the N-terminal ORF 1a proteins of the coronavirus MHV-A59 requires multiple proteolytic events. Virology 189: 274\u2013284.\u00a0\u00a0\u00a0 \u300824. Denison MR\u3001Zoltick PW\u3001Hughes SA\u3001Giangreco B\u3001Olson AL \u7b49\u3002 (1992) \u51a0\u72c0\u75c5\u6bd2 MHV-A59 \u7684 N \u7aef ORF 1a \u86cb\u767d\u7684\u7d30\u80de\u5167\u52a0\u5de5\u9700\u8981\u591a\u500b\u86cb\u767d\u6c34\u89e3\u4e8b\u4ef6\u3002\u75c5\u6bd2\u5b78 189\uff1a274-284\u3002\u3009View Article\u3000Google Scholar 25.van Hemert MJ, de Wilde AH, Gorbalenya AE, Snijder EJ (2008) The in Vitro RNA Synthesizing Activity of the Isolated Arterivirus Replication\/Transcription Complex Is Dependent on a Host Factor. J Biol Chem 283: 16525\u201316536.\u00a0\u00a0\u00a0 \u300825. van Hemert MJ\u3001de Wilde AH\u3001Gorbalenya AE\u3001Snijder EJ (2008) \u5206\u96e2\u7684\u52d5\u8108\u75c5\u6bd2\u8907\u88fd\/\u8f49\u9304\u8907\u5408\u7269\u7684\u9ad4\u5916 RNA \u5408\u6210\u6d3b\u6027\u53d6\u6c7a\u65bc\u5bbf\u4e3b\u56e0\u5b50\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 283\uff1a16525-16536\u3002\u3009View Article\u3000Google Scholar 26.van Hemert MJ, van den Worm SHE, Knoops K, Mommaas AM, Gorbalenya AE, et al. (2008) SARS-Coronavirus Replication\/Transcription Complexes Are Membrane-Protected and Need a Host Factor for Activity In Vitro. PLoS Pathog 4: e1000054.\u00a0\u00a0\u00a0 \u300826. van Hemert MJ\u3001van den Worm SHE\u3001Knoops K\u3001Mommaas AM\u3001Gorbalenya AE \u7b49\u3002 (2008) SARS-\u51a0\u72c0\u75c5\u6bd2\u8907\u88fd\/\u8f49\u9304\u8907\u5408\u7269\u53d7\u819c\u4fdd\u8b77\uff0c\u9700\u8981\u5bbf\u4e3b\u56e0\u5b50\u624d\u80fd\u9032\u884c\u9ad4\u5916\u6d3b\u52d5\u3002 PLoS \u75c5\u539f\u9ad4 4\uff1ae1000054\u3002\u3009View Article\u3000Google Scholar 27.te Velthuis AJ, Arnold JJ, Cameron CE, van den Worm SH, Snijder EJ (2009) The RNA polymerase activity of SARS-coronavirus nsp12 is primer dependent. Nucleic Acids Res 38: 203\u2013214.\u00a0\u00a0\u00a0 \u300827. te Velthuis AJ\u3001Arnold JJ\u3001Cameron CE\u3001van den Worm SH\u3001Snijder EJ (2009) SARS \u51a0\u72c0\u75c5\u6bd2 nsp12 \u7684 RNA \u805a\u5408\u9176\u6d3b\u6027\u4f9d\u8cf4\u65bc\u5f15\u7269\u3002\u6838\u9178\u7814\u7a76 38\uff1a203-214\u3002\u3009View Article\u3000Google Scholar 28.Beerens N, Selisko B, Ricagno S, Imbert I, van der Zanden L, et al. (2007) De Novo Initiation of RNA Synthesis by the Arterivirus RNA-Dependent RNA Polymerase. J Virol 81: 8384\u20138395.\u00a0\u00a0\u00a0 \u300828. Beerens N\u3001Selisko B\u3001Ricagno S\u3001Imbert I\u3001van der Zanden L \u7b49\u3002 (2007) \u901a\u904e\u52d5\u8108\u75c5\u6bd2 RNA \u4f9d\u8cf4\u7684 RNA \u805a\u5408\u9176\u5f9e\u982d\u958b\u59cb\u5408\u6210 RNA\u3002 J Virol 81\uff1a8384\u20138395\u3002\u3009View Article\u3000Google Scholar 29.van den Born E, Posthuma CC, Knoops K, Snijder EJ (2007) An infectious recombinant equine arteritis virus expressing green fluorescent protein from its replicase gene. J Gen Virol 88: 1196\u20131205.\u00a0\u00a0\u00a0 \u300829. van den Born E, Posthuma CC, Knoops K, Snijder EJ (2007) \u4e00\u7a2e\u50b3\u67d3\u6027\u91cd\u7d44\u99ac\u52d5\u8108\u708e\u75c5\u6bd2\uff0c\u5f9e\u5176\u8907\u5236\u9176\u57fa\u56e0\u8868\u9054\u7da0\u8272\u7192\u5149\u86cb\u767d\u3002 J Gen Virol 88\uff1a1196-1205\u3002\u3009View Article\u3000Google Scholar 30.Sims AC, Burkett SE, Yount B, Pickles RJ (2008) SARS-CoV replication and pathogenesis in an in vitro model of the human conducting airway epithelium. Virus Res 133: 33\u201344.\u00a0\u00a0\u00a0 \u300830. Sims AC\u3001Burkett SE\u3001Yount B\u3001Pickles RJ (2008) SARS-CoV \u5728\u4eba\u985e\u50b3\u5c0e\u6c23\u9053\u4e0a\u76ae\u9ad4\u5916\u6a21\u578b\u4e2d\u7684\u8907\u5236\u548c\u767c\u75c5\u6a5f\u5236\u3002\u75c5\u6bd2\u7814\u7a76 133\uff1a33-44\u3002\u3009View Article\u3000Google Scholar 31.Xu X, Liu Y, Weiss S, Arnold E, Sarafianos SG, et al. (2003) Molecular model of SARS coronavirus polymerase: implications for biochemical functions and drug design. Nucleic Acids Res 31: 7117\u20137130.\u00a0\u00a0\u00a0 \u300831. Xu X\u3001Liu Y\u3001Weiss S\u3001Arnold E\u3001Sarafianos SG \u7b49\u3002 (2003) SARS \u51a0\u72c0\u75c5\u6bd2\u805a\u5408\u9176\u7684\u5206\u5b50\u6a21\u578b\uff1a\u5c0d\u751f\u5316\u529f\u80fd\u548c\u85e5\u7269\u8a2d\u8a08\u7684\u5f71\u97ff\u3002\u6838\u9178\u7814\u7a76 31\uff1a7117-7130\u3002\u3009View Article\u3000Google Scholar 32.Zhai Y, Sun F, Li X, Pang H, Xu X, et al. (2005) Insights into SARS-CoV transcription and replication from the structure of the nsp7-nsp8 hexadecamer. Nat Struct Mol Biol 12: 980\u2013986.\u00a0\u00a0\u00a0 \u300832. \u7fdf\u6bc5\u3001\u5b6b\u98db\u3001\u674e\u65ed\u3001\u9f90\u83ef\u3001\u5f90\u65ed\u7b49\u3002 (2005) \u5f9e nsp7-nsp8 \u5341\u516d\u805a\u9ad4\u7684\u7d50\u69cb\u6d1e\u5bdf SARS-CoV \u8f49\u9304\u548c\u5fa9\u5236\u3002 Nat Struct Mol Biol 12\uff1a980-986\u3002\u3009View Article\u3000Google Scholar 33.Stockman LJ, Bellamy R, Garner P (2006) SARS: Systematic Review of Treatment Effects. PLoS Med 3: e343.\u00a0\u00a0\u00a0 \u300833. Stockman LJ\u3001Bellamy R\u3001Garner P (2006) SARS\uff1a\u6cbb\u7642\u6548\u679c\u7684\u7cfb\u7d71\u8a55\u50f9\u3002 PLoS Med 3: e343\u3002\u3009View Article\u3000Google Scholar 34.Thompson A, Patel K, Tillman H, McHutchison JG (2009) Directly acting antivirals for the treatment of patients with hepatitis C infection: A clinical development update addressing key future challenges. J Hepatol 50: 184\u2013194.\u00a0\u00a0\u00a0 \u300834. Thompson A\u3001Patel K\u3001Tillman H\u3001McHutchison JG (2009) \u7528\u65bc\u6cbb\u7642\u4e19\u578b\u809d\u708e\u611f\u67d3\u60a3\u8005\u7684\u76f4\u63a5\u6297\u75c5\u6bd2\u85e5\u7269\uff1a\u61c9\u5c0d\u672a\u4f86\u95dc\u9375\u6311\u6230\u7684\u81e8\u5e8a\u958b\u767c\u66f4\u65b0\u3002 J Hepatol 50\uff1a184-194\u3002\u3009View Article\u3000Google Scholar 35.De Clercq E (2004) Antivirals and antiviral strategies. Nat Rev Microbiol 2: 704\u2013720.\u00a0\u00a0\u00a0 \u300835. De Clercq E (2004) \u6297\u75c5\u6bd2\u85e5\u7269\u548c\u6297\u75c5\u6bd2\u7b56\u7565\u3002 Nat Rev Microbiol 2: 704\u2013720\u3002\u3009View Article\u3000Google Scholar 36.Thompson AJV, McHutchison JG (2009) Antiviral resistance and specifically targeted therapy for HCV (STAT-C). J Viral Hepat 16: 377\u2013387.\u00a0\u00a0\u00a0 \u300836. Thompson AJV, McHutchison JG (2009) HCV \u6297\u75c5\u6bd2\u8010\u85e5\u6027\u548c\u7279\u7570\u6027\u9776\u5411\u6cbb\u7642 (STAT-C)\u3002 J \u75c5\u6bd2\u6027\u809d\u75c5 16\uff1a377-387\u3002\u3009View Article\u3000Google Scholar 37.Yang W, Lee JY, Nowotny M (2006) Making and Breaking Nucleic Acids: Two-Mg2+-Ion Catalysis and Substrate Specificity. Mol Cell 22: 5\u201313.\u00a0\u00a0\u00a0 \u300837. Yang W\u3001Lee JY\u3001Nowotny M (2006) \u88fd\u9020\u548c\u7834\u58de\u6838\u9178\uff1a\u96d9 Mg2+ \u96e2\u5b50\u50ac\u5316\u548c\u5e95\u7269\u7279\u7570\u6027\u3002\u6469\u723e\u7d30\u80de 22\uff1a5-13\u3002\u3009View Article\u3000Google Scholar 38.Castro C, Smidansky E, Maksimchuk KR, Arnold JJ, Korneeva VS, et al. (2007) Two proton transfers in the transition state for nucleotidyl transfer catalyzed by RNA- and DNA-dependent RNA and DNA polymerases. Proc Natl Acad Sci USA 104: 4267\u20134272.\u00a0\u00a0\u00a0 \u300838. Castro C\u3001Smidansky E\u3001Maksimchuk KR\u3001Arnold JJ\u3001Korneeva VS \u7b49\u3002 (2007) \u7531 RNA \u548c DNA \u4f9d\u8cf4\u6027 RNA \u548c DNA \u805a\u5408\u9176\u50ac\u5316\u7684\u6838\u82f7\u9178\u8f49\u79fb\u7684\u5169\u500b\u904e\u6e21\u614b\u8cea\u5b50\u8f49\u79fb\u3002 Proc Natl Acad Sci USA 104\uff1a4267\u20134272\u3002\u3009View Article\u3000Google Scholar 39.Arnold JJ, Ghosh SK, Cameron CE (1999) Poliovirus RNA-dependent RNA Polymerase (3Dpol). Divalent cation modulation of primer, template and nucleotide selection. J Biol Chem 274: 37060\u201337069.\u00a0\u00a0\u00a0 \u300839. Arnold JJ\u3001Ghosh SK\u3001Cameron CE (1999) \u810a\u9ad3\u7070\u8cea\u708e\u75c5\u6bd2 RNA \u4f9d\u8cf4\u6027 RNA \u805a\u5408\u9176 (3Dpol)\u3002\u5f15\u7269\u3001\u6a21\u677f\u548c\u6838\u82f7\u9178\u9078\u64c7\u7684\u4e8c\u50f9\u967d\u96e2\u5b50\u8abf\u7bc0\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 274\uff1a37060-37069\u3002\u3009View Article\u3000Google Scholar 40.Iuchi S (2001) Three classes of C2H2 zinc finger proteins. Cell Mol Life Sci 58: 625\u2013635.\u00a0\u00a0\u00a0 \u300840. Iuchi S (2001) \u4e09\u985e C2H2 \u92c5\u6307\u86cb\u767d\u3002\u7d30\u80de\u5206\u5b50\u751f\u547d\u79d1\u5b78 58\uff1a625-635\u3002\u3009View Article\u3000Google Scholar 41.Gomis-Ruth XF (2009) Catalytic domain architecture of metzincin metalloproteases. J Biol Chem 284: 15353\u201315357.\u00a0\u00a0\u00a0 \u300841. Gomis-Ruth XF (2009) Metzincin \u91d1\u5c6c\u86cb\u767d\u9176\u7684\u50ac\u5316\u57df\u7d50\u69cb\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 284\uff1a15353\u201315357\u3002\u3009View Article\u3000Google Scholar 42.Yap TL, Xu T, Chen Y-L, Malet H, Egloff M-P, et al. (2007) Crystal Structure of the Dengue Virus RNA-Dependent RNA Polymerase Catalytic Domain at 1.85-Angstrom Resolution. J Virol 81: 4753\u20134765.\u00a0\u00a0\u00a0 \u300842. Yap TL\u3001Xu T\u3001Chen Y-L\u3001Malet H\u3001Egloff M-P \u7b49\u3002 (2007) \u5206\u8fa8\u7387\u70ba 1.85 \u57c3\u7684\u767b\u9769\u71b1\u75c5\u6bd2\u4f9d\u8cf4\u65bc RNA \u7684 RNA \u805a\u5408\u9176\u50ac\u5316\u7d50\u69cb\u57df\u7684\u6676\u9ad4\u7d50\u69cb\u3002 J Virol 81\uff1a4753\u20134765\u3002\u3009View Article\u3000Google Scholar 43.Winek CL, Buehler EV (1966) Intravenous toxicity of zinc pyridinethione and several zinc salts. Toxicol Appl Pharmacol 9: 296\u2013273.\u00a0\u00a0\u00a0 \u300843. Winek CL, Buehler EV (1966) \u5421\u5576\u786b\u916e\u92c5\u548c\u5e7e\u7a2e\u92c5\u9e7d\u7684\u975c\u8108\u6bd2\u6027\u3002 Toxicol Appl Pharmacol 9\uff1a296-273\u3002\u3009View Article\u3000Google Scholar 44.Snyder DR, de Jesus CP, Towfighi J, Jacoby RO, Wedig JH (1979) Neurological, microscopic and enzyme-histochemical assessment of zinc pyrithione toxicity. Food Cosmet Toxicol 17: 651\u2013660.\u00a0\u00a0\u00a0 \u300844. Snyder DR, de Jesus CP, Towfighi J, Jacoby RO, Wedig JH (1979) \u5421\u5576\u786b\u916e\u92c5\u6bd2\u6027\u7684\u795e\u7d93\u5b78\u3001\u986f\u5fae\u93e1\u548c\u9176\u7d44\u7e54\u5316\u5b78\u8a55\u4f30\u3002\u98df\u54c1\u5316\u599d\u54c1\u6bd2\u7406\u5b78 17\uff1a651-660\u3002\u3009View Article\u3000Google Scholar 45.Magda D, Lecane P, Wang Z, Hu W, Thiemann P, et al. (2008) Synthesis and anticancer properties of water-soluble zinc ionophores. Cancer Res 68: 5318\u20135325.\u00a0\u00a0\u00a0 \u300845. Magda D\u3001Lecane P\u3001Wang Z\u3001Hu W\u3001Thiemann P \u7b49\u3002 (2008) \u6c34\u6eb6\u6027\u92c5\u96e2\u5b50\u8f09\u9ad4\u7684\u5408\u6210\u548c\u6297\u764c\u7279\u6027\u3002\u764c\u75c7\u7814\u7a76 68\uff1a5318-5325\u3002\u3009View Article\u3000oogle Scholar 46.Snijder EJ, van der Meer Y, Zevenhoven-Dobbe J, Onderwater JJM, van der Meulen J, et al. (2006) Ultrastructure and Origin of Membrane Vesicles Associated with the Severe Acute Respiratory Syndrome Coronavirus Replication Complex. J Virol 80: 5927\u20135940.\u00a0\u00a0\u00a0 \u300846. Snijder EJ\u3001van der Meer Y\u3001Zevenhoven-Dobbe J\u3001Onderwater JJM\u3001van der Meulen J \u7b49\u3002 (2006) \u8207\u56b4\u91cd\u6025\u6027\u547c\u5438\u7cfb\u7d71\u7d9c\u5408\u75c7\u51a0\u72c0\u75c5\u6bd2\u8907\u88fd\u8907\u5408\u9ad4\u76f8\u95dc\u7684\u819c\u56ca\u6ce1\u7684\u8d85\u5fae\u7d50\u69cb\u548c\u8d77\u6e90\u3002 J Virol 80\uff1a5927-5940\u3002\u3009View Article\u3000Google Scholar 47.Studier F (2005) Protein production by auto-induction in high density shaking cultures. Protein Expr Purif 41: 207\u2013234.\u00a0\u00a0\u00a0 \u300847. Studier F (2005) \u5728\u9ad8\u5bc6\u5ea6\u632f\u76ea\u57f9\u990a\u4e2d\u901a\u904e\u81ea\u52d5\u8a98\u5c0e\u7522\u751f\u86cb\u767d\u8cea\u3002\u86cb\u767d\u8cea Expr Purif 41\uff1a207-234\u3002\u3009View Article\u3000Google Scholar   \u91cd\u5fb7\u4e26\u4e14\u5c07\u5728\u5317\u4eac\u7684\u300c\u4e2d\u570b\u79d1\u5b78\u9662\u751f\u7269\u7269\u7406\u7814\u7a76\u6240\u611f\u67d3\u8207\u514d\u75ab\u5b78\u91cd\u9ede\u5be6\u9a57\u5ba4\u300d\u5728\u91ab\u5b78\u96dc\u8a8c\u4e0a\u6240\u767c\u8868\u7684\u4e00\u7bc7\u82f1\u6587\u7814\u7a76\u5831\u544a\u300a\u92c5\u6307\u6297\u75c5\u6bd2\u86cb\u767d\u6291\u5236\u7532\u578b\u6d41\u611f\u75c5\u6bd2\u86cb\u767d\u8868\u9054\u4ee5\u53ca\u88ab\u75c5\u6bd2\u7de8\u78bcNS1 \u5c0d\u6297\u7684\u7c21\u8981\u5f62\u5f0f\u300b\u7684\u4e2d\u6587\u81ea\u8b6f\u7248\u4e5f\u5bc4\u4e0a\u7d66\u4ed6\u53c3\u8003(\u5982\u9644\u6a94)\u3002 \u4e26\u4e14\uff0c\u91cd\u5fb7\u9084\u767c\u53bb\u4ee5\u4e0b\u5716\u7247\u8207\u8aaa\u660e\uff1a   \u300e\u9019\u5c31\u662f\u92c5\u6307\u7d50\u69cb\u5716\uff0c\u7531\u65bc\u9019\u662f\u87af\u5408\u7d50\u69cb\uff0c\u56e0\u6b64\u5b83\u80fd\u5f88\u5bb9\u6613\u5c07\u92c5\u96e2\u5b50\u7a7f\u900f\u7d30\u80de\u58c1\u800c\u8207\u8eb2\u85cf\u5728\u7d30\u80de\u5167\u840c\u767c\u7684\u75c5\u6bd2\u56e0\u70ba\u767c\u751f\u6291\u5236\u7ffb\u8b6f\u548c\u4fc3\u9032\u75c5\u6bd2 mRNA\u7684\u964d\u89e3\uff0c\u56e0\u6b64\u901a\u904e PARP \u7d50\u69cb\u57df\u800c\u4fc3\u9032 IAV \u86cb\u767d\u964d\u89e3\uff0c\u5be6\u9a57\u5df2\u7d93\u8b49\u5be6\u5b83\u53ef\u8b93\u5165\u4fb5\u5230\u7d30\u80de\u5167\u7684\u75c5\u6bd2\u4e4bRNA\u6a5f\u8f49\u767c\u751f\u4f11\u514b(\u4f46\u4e0d\u4e00\u5b9a\u6703\u6b7b\u4ea1)\uff0c\u800c\u4f7f\u5f97\u539f\u672c\u7121\u751f\u547d\u7684\u75c5\u6bd2\u56e0\u6b64\u4e0d\u80fd\u5927\u91cf\u8907\u88fd\u840c\u751f\uff0c\u800c\u53ef\u4ee5\u70ba\u4eba\u9ad4\u7684\u514d\u75ab\u7cfb\u7d71\u89e3\u6c7a\u3002\u300f \u9019\u4e00\u4f4d\u738b\u59d3\u7db2\u53cb\u53c8\u767c\u4f86\u4ee5\u4e0b\u4fe1\u606f\uff1a\u300e\u80fd\u5920\u6bba\u75c5\u6bd2\u800c\u4e0d\u6703\u6bba\u6b7b\u6211\u5011\u81ea\u5df1\u7684\u7d30\u80de,\u5929\u4e0b\u6709\u9019\u9ebc\u597d\u7684\u4e8b\u55ce\uff1f\u4e0d\u904e\u662f\u71df\u990a\u88dc\u52a9\u54c1\u800c\u5df2\uff01\u300f \u91cd\u5fb7\u770b\u4ed6\u5728\u610f\u6c23\u7528\u4e8b\uff0c\u56e0\u6b64\u5c31\u56de\u8986\u4ed6\u8aaa\uff1a\u300e\u96a8\u60a8\u9ad8\u8208\u600e\u9ebc\u8aaa\u90fd\u597d\uff0c\u6211\u6c92\u6709\u8aaa\u5b83\u662f\u85e5\uff0c\u53ea\u8aaa\u662f\u4fdd\u5065\u54c1\u800c\u5df2\u3002\u300f\u767c\u5b8c\u4e86\u9019\u4fe1\u606f\u4e4b\u5f8c\uff0c\u91cd\u5fb7\u767c\u8a93\u4ee5\u5f8c\u518d\u4e5f\u4e0d\u5c0d\u9019\u7a2e\u96de\u86cb\u88e1\u6311\u9aa8\u982d\u7684\u4eba\u63d0\u4f9b\u4efb\u4f55\u6709\u50f9\u503c\u7684\u8cc7\u8a0a\u4e86\u3002 \u4f46\u662f\uff0c\u91cd\u5fb7\u9084\u662f\u8981\u544a\u8a34\u5927\u5bb6\u70ba\u4f55\u8981\u5efa\u8b70\u5927\u5bb6\u5403\u542b\u92c5\u96e2\u5b50\u7684\u4fdd\u5065\u98df\u54c1\uff0c\u7576\u7136\u92c5\u5f9e\u98df\u7269\u4e2d\u651d\u53d6\u4e5f\u6709\uff0c\u4f46\u662f\u98df\u7269\u5fc5\u9808\u7d93\u904e\u6d88\u5316\u5f8c\u92c5\u96e2\u5b50\u624d\u6703\u900f\u904e\u8840\u6db2\u800c\u5230\u4eba\u9ad4\u5668\u5b98\uff0c\u9019\u500b\u904e\u7a0b\u592a\u9577\u592a\u4e45\u4e86\uff0c\u56e0\u6b64\u92c5\u96e2\u5b50\u53ef\u80fd\u53c8\u8207\u5176\u4ed6\u7269\u8cea\u7d50\u5408\u6210\u5316\u5408\u7269\uff0c\u800c\u518d\u4e0d\u80fd\u767c\u751f\u8207\u8ca0\u96e2\u5b50\u7684\u75c5\u6bd2\u4e2d\u548c\u7684\u6548\u679c\u3002\u56e0\u6b64\u5c31\u66f4\u6c92\u6709\u767c\u63ee\u5230\u92c5\u96e2\u5b50\u6240\u7368\u5177\u7684\u87af\u5408\u4ea4\u63db\u6548\u679c\u3002 \u7531\u65bc\uff0c\u611f\u5192\u6216\u662fCOVID-19\u7684\u521d\u671f\u75c5\u72c0\u4e3b\u8981\u90fd\u662f\u91dd\u5c0d\u4e0a\u652f\u6c23\u7ba1\uff0c\u56e0\u6b64\u4ee3\u8868\u547c\u5438\u7ba1\u9053\u7684\u75c5\u6bd2\u91cf\u6700\u5927\uff0c\u56e0\u6b64\u53ef\u4ee5\u542b\u5728\u5634\u4e2d\u6162\u6162\u5206\u89e3\u7684\u5589\u7cd6\u4e4b\u6548\u679c\u6700\u597d\uff0c\u56e0\u70ba\u5b83\u8b93\u901a\u904e\u7684\u6c23\u6d41\u4e2d\u7684\u75c5\u6bd2\u5148\u88ab\u92c5\u96e2\u5b50\u4e2d\u548c\uff0c\u800c\u7cd6\u6c41\u6d41\u5165\u98df\u9053\u6642\u92c5\u96e2\u5b50\u4e5f\u6703\u6ea2\u5165\u4e0a\u652f\u6c23\u7ba1\u3002\u9019\u5c31\u662f\u70ba\u4f55\u91cd\u5fb7\u9996\u5148\u63a8\u85a6\u5df2\u7d93\u5728\u7f8e\u570b\u98a8\u884c30\u591a\u5e74\u7684\u611f\u5192\u9810\u9632\u7528\u85e5Cold Eeze\u4e2d\u6587\u8b6f\u540d\u70ba\u3010\u92c5\u7cd6\u3011\uff0c\u56e0\u70ba\u5b83\u5df2\u7d93\u904e\u4e86\u5c08\u5229\u4fdd\u8b77\u671f\uff0c\u5b78\u540d\u85e5\u540d\u662fGeneric Version\uff0c\u56e0\u70ba\u552e\u50f9\u8f03\u4fbf\u5b9c(\u4f46\u662f\u73fe\u5728\u5168\u7403\u6436\u8cfc\u5f88\u53ef\u80fd\u7f3a\u8ca8)\u3002 \u73fe\u5728\uff0c\u91cd\u5fb7\u8981\u544a\u8a34\u5927\u5bb6\u4e00\u500b\u79d8\u5bc6\uff0c\u90a3\u5c31\u662f\u70ba\u4f55\u91cd\u5fb7\u8981\u5c0d\u92c5\u96e2\u5b50\u9032\u884c\u7814\u7a76\u5462\uff0c\u6545\u4e8b\u662f\u9019\u6a23\u5b50\u7684\uff0c\u7531\u65bc\u4e00\u5e74\u524d \u91cd\u5fb7 \u8acb\u793a\u201c\u9748\u7236\u201d(\u5143\u59cb\u5929\u5c0a)\u8aaa\uff1a\u300e\u65e2\u7136\u00a0 \u9748\u7236\u80fd\u63d0\u524d\u534a\u5e74\u8b66\u544a\u6211\u5011\u5c07\u6709\u6b64\u5927\u75ab\u60c5\uff0c\u662f\u5426\u80fd\u544a\u77e5\u5f92\u5152\u5982\u4f55\u88fd\u5f97\u89e3\u85e5\uff1f\u300f\u64f2\u832d\u61c9\u8a31\u5f8c\uff0c\u91cd\u5fb7 \u6b63\u4e0d\u77e5\u5982\u4f55\u518d\u554f\u9019\u89e3\u85e5\u79d8\u65b9\u6642\uff0c\u8166\u4e2d\u7a81\u7136\u6e67\u73fe\u4e00\u500b\u201c\u92c5\u201d\u5b57\uff0c\u64f2\u832d\u518d\u554f\u78ba\u5b9a\u795e\u8aed\u5c31\u662f\u6b64\u201c\u92c5\u201d\u5b57\u7121\u8aa4\u3002 \u5728\u6b64\u4e4b\u5f8c\uff0c\u91cd\u5fb7\u624d\u958b\u59cb\u8a73\u95b1\u5404\u7a2e\u82f1\u6587\u91ab\u5b78\u6587\u737b\uff0c\u8d8a\u767c\u8b49\u5be6\u92c5\u96e2\u5b50\u6709\u6291\u5236mRNA\u75c5\u6bd2\u7684\u6548\u679c\uff0c\u800c\u4e14\u56e0\u70ba\u92c5\u96e2\u5b50\u662f\u63a1\u53d6\u87af\u5408\u65b9\u5f0f\u8b93\u92c5\u96e2\u5b50\u7a7f\u900f\u7d30\u80de\u58c1\u9032\u5165\u4f86\u964d\u89e3\u75c5\u6bd2\u86cb\u767d\u8907\u88fd\u6240\u9700\u6c42\u7684PARP \u7d50\u69cb\u57df\uff0c\u56e0\u6b64\u9019\u662f\u4e00\u7a2e\u7cbe\u6e96\u7834\u7a97\u5f0f\u7684\u65ac\u9996\u884c\u52d5\uff0c\u5c0d\u65bc\u7d30\u80de\u7684\u50b7\u5bb3\u751a\u5c0f\uff0c\u56e0\u6b64\u5c0d\u65bc\u4eba\u9ad4\u7684\u5371\u5bb3\u751a\u4f4e\u3002 \u91cd\u5fb7\u4e00\u5e74\u524d\u5c31\u628a\u7814\u7a76\u6210\u679c\u516c\u958b\uff0c\u4e00\u4f86\u56e0\u70ba\u81ea\u5df1\u962e\u56ca\u7f9e\u6f80\uff0c\u7121\u6cd5\u7e7c\u7e8c\u7814\u767c\uff0c\u4e8c\u4f86\u91cd\u5fb7\u5df2\u7d93\u9810\u7aba\u5230\u672a\u4f86\u9084\u6709\u5176\u4ed6\u56b4\u91cd\u75be\u75c5\uff0c\u800c\u4e14COVID-19\u9084\u6703\u5927\u7a81\u8b8a\uff0c\u56e0\u6b64\u8b93\u5176\u4ed6\u4eba\u63a5\u68d2\u4f86\u7814\u7a76\u6216\u8a31\u6703\u66f4\u6709\u6210\u6548\u3002\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/pingtungtimes.com.tw\/?p=104514\" \/>\n<meta property=\"og:locale\" content=\"zh_TW\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"\u597d\u201c\u92c5\u201d\u3000\u4e5f\u6703\u60f9\u4e86\u4e00\u8eab\u8165 - \u5c4f\u6771\u6642\u5831\u96fb\u5b50\u5831\" \/>\n<meta property=\"og:description\" content=\"\u3010\u674e\u91cd\u5fb7\uff0f\u5c08\u6b04\u5831\u5c0e2022\/05\/27\u3011\u3000\u4eca\u65e9\uff0c\u6709\u4e00\u78ba\u8a3a\u7652\u5f8c\u8005\u8207\u91cd\u5fb7\u8a0e\u8ad6\u4ed6\u7684\u75c5\u60c5\uff0c\u91cd\u5fb7\u5efa\u8b70\u4ed6\u88dc\u5145\u92c5\u96e2\u5b50\u98df\u54c1\uff0c\u4e26\u5c07\u6b64\u4e00\u4fe1\u606f\u4e5f\u767c\u7d66\u5927\u5bb6\u77e5\u66c9\uff1a \u300e\u6709\u78ba\u8a3a\u7652\u5f8c\u8005\u554f\u91cd\u5fb7\u8981\u5982\u4f55\u4fdd\u5065\u4ee5\u9632\u5fa9\u967d\uff0c\u91cd\u5fb7 \u7686\u5efa\u8b70\u4ed6\u5403\u3010\u92c5\u7cd6\u3011(\u985e\u4f3c\u5589\u7247)\u5c0f\u5b69\u5247\u5403\u3010\u5152\u7ae5\u92c5\u8edf\u7cd6\u3011\uff0c\u6216\u662f\u3010\u9175\u6bcd\u92c5\u3011\u3001\u3010\u87af\u5408\u92c5\u3011\u6216\u662f\u3010\u8461\u8404\u7cd6\u9178\u92c5\u3011\u4f86\u6d88\u6ec5\u6f5b\u85cf\u4eba\u9ad4\u5c1a\u672a\u767c\u4f5c\u7684\u75c5\u6bd2\u3002\u300f \u5f8c\u4f86\u5c31\u63a5\u7372\u4e00\u4f4d\u738b\u59d3\u7db2\u53cb\u56de\u8986\u8aaa\uff1a\u300e\u4e0d\u5c0d\u5594\uff01\u92c5,\u4eba\u985e\u5b58\u6d3b\u6240\u9700\u8981\u7684\u5143\u7d20\u4e4b\u4e00,\u5f9e\u5929\u7136\u98df\u7269\u76f4\u63a5\u651d\u53d6\u5c31\u8db3\u5920\u4e86,\u4eba\u70ba\u8d85\u91cf\u7684\u88dc\u5145\u6703\u51fa\u554f\u984c\u7684\uff01\u92c5,\u4e0d\u6703\u76f4\u63a5\u6bba\u75c5\u6bd2\u7684\uff01\u300f \u9019\u4e00\u4f4d\u738b\u5148\u751f\uff0c\u5927\u6982\u6f0f\u770b\u4e86\u91cd\u5fb7\u5728\u4e00\u5e74\u524d\u5728\u672c\u6703\u7fa4\u7d44\u4e0a\u767c\u8868\u7684\u5e7e\u7bc7\u4ee5\u92c5\u96e2\u5b50\u4f86\u6d88\u6ec5\u75c5\u6bd2\u7684\u6587\u7ae0\uff0c\u56e0\u6b64\uff0c\u91cd\u5fb7\u5c31\u56de\u8986\u4ed6\u8aaa\uff1a\u300e\u6211\u770b\u904e\u4e86\u8a31\u591a\u82f1\u6587\u91ab\u5b78\u6587\u737b\uff0c\u4e5f\u8207\u9ec3\u25cb\u5bcc\u535a\u58eb\u548c\u9673\u25cb\u5e73\u91ab\u5e2b\u8a0e\u8ad6\u904e\u3002\u60a8\u5982\u679c\u60f3\u8981\u770b\u9019\u4e00\u4e9b\u82f1\u6587\u91ab\u5b78\u6587\u737b\uff0c\u6211\u53ef\u4ee5\u5bc4\u53bb\u7d66\u60a8\u3002\u300f\u7136\u5f8c \u91cd\u5fb7 \u5c31\u5c31\u5bc4\u53bb\u7d66\u4ed6\u4ee5\u4e0b\u7684\u8cc7\u6599\uff1a References\u53c3\u8003\u6587\u737b 1.Lazarczyk M, Favre M (2008) Role of Zn2+ ions in host-virus interactions. J Virol 82: 11486\u201311494.\u00a0\u00a0\u00a0 \u30081. Lazarczyk M, Favre M (2008) Zn2+ \u96e2\u5b50\u5728\u5bbf\u4e3b-\u75c5\u6bd2\u76f8\u4e92\u4f5c\u7528\u4e2d\u7684\u4f5c\u7528\u3002 J Virol 82\uff1a11486\u201311494\u3002\u3009\u3000View Article\u3000Google Scholar 2.Frederickson CJ, Koh JY, Bush AI (2005) Neurobiology of zinc in health and disease. Nat Rev Neurosci 6: 449\u2013462.\u00a0\u00a0\u00a0 \u30082. Frederickson CJ\u3001Koh JY\u3001Bush AI (2005) \u92c5\u5728\u5065\u5eb7\u548c\u75be\u75c5\u4e2d\u7684\u795e\u7d93\u751f\u7269\u5b78\u3002 Nat Rev Neurosci 6\uff1a449-462\u3002\u3009View Article\u3000Google Scholar 3.Alirezaei M, Nairn AC, Glowinski J, Premont J, Marin P (1999) Zinc inhibits protein synthesis in neurons: potential rol of phosphorylation of translation initiation factor-2a. J Biol Chem 274: 32433\u201332438.\u00a0\u00a0\u00a0 \u30083. Alirezaei M\u3001Nairn AC\u3001Glowinski J\u3001Premont J\u3001Marin P (1999) \u92c5\u6291\u5236\u795e\u7d93\u5143\u4e2d\u7684\u86cb\u767d\u8cea\u5408\u6210\uff1a\u7ffb\u8b6f\u8d77\u59cb\u56e0\u5b50 2a \u78f7\u9178\u5316\u7684\u6f5b\u5728\u4f5c\u7528\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 274\uff1a32433\u201332438\u3002\u3009View Article\u3000Google Scholar 4.Uchide N, Ohyama K, Bessho T, Yuan B, Yamakawa T (2002) Effect of antioxidants on apoptosis induced by influenza virus infection: inhibition of viral gene replication and transcription with pyrrolidine dithiocarbamate. Antiviral Res 56: 207\u2013217.\u00a0\u00a0\u00a0 \u30084. Uchide N, Ohyama K, Bessho T, Yuan B, Yamakawa T (2002) \u6297\u6c27\u5316\u5291\u5c0d\u6d41\u611f\u75c5\u6bd2\u611f\u67d3\u8a98\u5c0e\u7684\u7d30\u80de\u51cb\u4ea1\u7684\u5f71\u97ff\uff1a\u7528\u5421\u54af\u70f7\u4e8c\u786b\u4ee3\u6c28\u57fa\u7532\u9178\u916f\u6291\u5236\u75c5\u6bd2\u57fa\u56e0\u8907\u5236\u548c\u8f49\u9304\u3002\u6297\u75c5\u6bd2\u7814\u7a76 56\uff1a207-217\u3002\u3009View Article\u3000Google Scholar 5.Suara RO, Crowe JEJ (2004) Effect of zinc salts on respiratory syncytial virus replication. Antimicrob Agents Chemother 48: 783\u2013790.\u00a0\u00a0\u00a0 \u30085. Suara RO, Crowe JEJ (2004) \u92c5\u9e7d\u5c0d\u547c\u5438\u9053\u5408\u80de\u75c5\u6bd2\u8907\u88fd\u7684\u5f71\u97ff\u3002\u6297\u5fae\u751f\u7269\u5291 Chemother 48\uff1a783-790\u3002\u3009View Article\u3000Google Scholar 6.Gaudernak E, Seipelt J, Triendl A, Grassauer A, Kuechler E (2002) Antiviral Effects of Pyrrolidine Dithiocarbamate on Human Rhinoviruses. J Virol 76: 6004\u20136015.\u00a0\u00a0\u00a0 \u30086. Gaudernak E\u3001Seipelt J\u3001Triendl A\u3001Grassauer A\u3001Kuechler E (2002) \u5421\u54af\u70f7\u4e8c\u786b\u4ee3\u6c28\u57fa\u7532\u9178\u916f\u5c0d\u4eba\u9f3b\u75c5\u6bd2\u7684\u6297\u75c5\u6bd2\u4f5c\u7528\u3002 J Virol 76\uff1a6004-6015\u3002\u3009View Article\u3000Google Scholar 7.Si X, McManus BM, Zhang J, Yuan J, Cheung C, et al. (2005) Pyrrolidine Dithiocarbamate Reduces Coxsackievirus B3 Replication through Inhibition of the Ubiquitin-Proteasome Pathway. J Virol 79: 8014\u20138023.\u00a0\u00a0\u00a0 \u30087. Si X, McManus BM, Zhang J, Yuan J, Cheung C, et al. (2005) Pyrrolidine Dithiocarbamate \u901a\u904e\u6291\u5236\u6cdb\u7d20-\u86cb\u767d\u9176\u9ad4\u9014\u5f91\u6e1b\u5c11\u67ef\u85a9\u5947\u75c5\u6bd2 B3 \u8907\u88fd\u3002 J Virol 79\uff1a8014-8023\u3002\u3009View Article\u3000Google Scholar 8.Korant BD, Kauer JC, Butterworth BE (1974) Zinc ions inhibit replication of rhinoviruses. Nature 248: 588\u2013590.\u00a0\u00a0\u00a0 \u30088. Korant BD, Kauer JC, Butterworth BE (1974) \u92c5\u96e2\u5b50\u6291\u5236\u9f3b\u75c5\u6bd2\u7684\u8907\u5236\u3002\u81ea\u7136 248\uff1a588-590\u3002\u3009View Article\u3000Google Scholar 9.Polatnick J, Bachrach HL (1978) Effect of zinc and other chemical agents on foot-and-mouth-disease virus replication. Antimicrob Agents Chemother 13: 731\u2013734.\u00a0\u00a0 \u00a0\u30089. Polatnick J, Bachrach HL (1978) \u92c5\u548c\u5176\u4ed6\u5316\u5b78\u8a66\u5291\u5c0d\u53e3\u8e44\u75ab\u75c5\u6bd2\u8907\u88fd\u7684\u5f71\u97ff\u3002\u6297\u5fae\u751f\u7269\u5291 Chemother 13\uff1a731-734\u3002\u3009View Article\u3000Google Scholar 10.Lanke K, Krenn BM, Melchers WJG, Seipelt J, van Kuppeveld FJM (2007) PDTC inhibits picornavirus polyprotein processing and RNA replication by transporting zinc ions into cells. J Gen Virol 88: 1206\u20131217.\u00a0\u00a0\u00a0 \u300810. Lanke K\u3001Krenn BM\u3001Melchers WJG\u3001Seipelt J\u3001van Kuppeveld FJM (2007) PDTC \u901a\u904e\u5c07\u92c5\u96e2\u5b50\u8f49\u904b\u5230\u7d30\u80de\u4e2d\u4f86\u6291\u5236\u5c0f\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u591a\u86cb\u767d\u52a0\u5de5\u548c RNA \u8907\u88fd\u3002 J Gen Virol 88\uff1a1206-1217\u3002\u3009View Article\u3000Google Scholar7 11.Krenn BM, Gaudernak E, Holzer B, Lanke K, Van Kuppeveld FJM, et al. (2009) Antiviral Activity of the Zinc Ionophores Pyrithione and Hinokitiol against Picornavirus Infections. J Virol 83: 58\u201364.\u00a0\u00a0\u00a0 \u300811. Krenn BM\u3001Gaudernak E\u3001Holzer B\u3001Lanke K\u3001Van Kuppeveld FJM \u7b49\u3002 (2009) \u92c5\u96e2\u5b50\u8f09\u9ad4\u5421\u5576\u786b\u916e\u548c\u6a9c\u9187\u5c0d\u5c0f\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u611f\u67d3\u7684\u6297\u75c5\u6bd2\u6d3b\u6027\u3002 J Virol 83\uff1a58-64\u3002\u3009View Article\u3000Google Scholar 12.Zalewski PD, Forbes IJ, Betts WH (1993) Correlation of apoptosis with change in intracellular labile Zn(II) using Zinquin , a new specific fluorescent probe for Zn(II). Biochem J 296: 403\u2013408.\u00a0 \u300812. Zalewski PD, Forbes IJ, Betts WH (1993) \u4f7f\u7528 Zinquin \uff0c\u7d30\u80de\u51cb\u4ea1\u8207\u7d30\u80de\u5167\u4e0d\u7a69\u5b9a\u92c5\uff08II\uff09\u8b8a\u5316\u7684\u76f8\u95dc\u6027\uff0c\u4e00\u7a2e\u65b0\u7684\u7279\u7570\u6027Zn(II) \u7684\u7192\u5149\u63a2\u91dd\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 296\uff1a403-408\u3002\u3009View Article\u3000Google Scholar 13.Baum EZ, Bebernitz GA, Palant O, Mueller T, Plotch SJ (1991) Purification, properties, and mutagenesis of poliovirus 3C protease. Virology 165: 140\u2013150.\u00a0\u00a0\u00a0 \u300813. Baum EZ\u3001Bebernitz GA\u3001Palant O\u3001Mueller T\u3001Plotch SJ (1991) \u810a\u9ad3\u7070\u8cea\u708e\u75c5\u6bd2 3C \u86cb\u767d\u9176\u7684\u7d14\u5316\u3001\u7279\u6027\u548c\u8a98\u8b8a\u3002\u75c5\u6bd2\u5b78 165\uff1a140-150\u3002\u3009View Article\u3000Google Scholar 14.Cordingley MG, Register RB, Callahan PL, Garsky VM, Colonno RJ (1989) Cleavage of small peptides in vitro by human rhinovirus 14 3C protease expressed in Escherichia coli. J Virol 63: 5037\u20135045.\u00a0\u00a0\u00a0 \u300814. Cordingley MG\u3001Register RB\u3001Callahan PL\u3001Garsky VM\u3001Colonno RJ (1989) \u5728\u5927\u8178\u687f\u83cc\u4e2d\u8868\u9054\u7684\u4eba\u9f3b\u75c5\u6bd2 14 3C \u86cb\u767d\u9176\u9ad4\u5916\u88c2\u89e3\u5c0f\u80bd\u3002 J Virol 63\uff1a5037-5045\u3002\u3009View Article\u3000Google Scholar 15.Ferrari E, Wright-Minogue J, Fang JW, Baroudy BM, Lau JY, et al. (1999) Characterization of soluble hepatitis C virus RNA-dependent RNA polymerase expressed in Escherichia coli. J Virol 73: 1649\u20131654.\u00a0\u00a0\u00a0 \u300815. Ferrari E\u3001Wright-Minogue J\u3001Fang JW\u3001Baroudy BM\u3001Lau JY \u7b49\u3002 (1999) \u5728\u5927\u8178\u687f\u83cc\u4e2d\u8868\u9054\u7684\u53ef\u6eb6\u6027\u4e19\u578b\u809d\u708e\u75c5\u6bd2 RNA \u4f9d\u8cf4\u6027 RNA \u805a\u5408\u9176\u7684\u8868\u5fb5\u3002 J Virol 73\uff1a1649-1654\u3002\u3009View Article\u3000Google Scholar 16.Hung M, Gibbs CS, Tsiang M (2002) Biochemical characterization of rhinovirus RNA-dependent RNA polymerase. Antiviral Res 56: 99\u2013114.\u00a0\u00a0\u00a0 \u300816. Hung M\u3001Gibbs CS\u3001Tsiang M (2002) \u9f3b\u75c5\u6bd2 RNA \u4f9d\u8cf4\u6027 RNA \u805a\u5408\u9176\u7684\u751f\u5316\u7279\u5fb5\u3002\u6297\u75c5\u6bd2\u7814\u7a76 56\uff1a99-114\u3002\u3009View Article\u3000Google Scholar 17.Perlman S, Netland J (2009) Coronaviruses post-SARS: update on replication and pathogenesis. Nat Rev Micro 7: 439\u2013450.\u00a0\u00a0\u00a0 \u300817. Perlman S, Netland J (2009) SARS \u5f8c\u7684\u51a0\u72c0\u75c5\u6bd2\uff1a\u8907\u88fd\u548c\u767c\u75c5\u6a5f\u5236\u7684\u66f4\u65b0\u3002 Nat Rev Micro 7\uff1a439-450\u3002\u3009View Article\u3000Google Scholar 18.Gorbalenya AE, Enjuanes L, Ziebuhr J, Snijder EJ (2006) Nidovirales: evolving the largest RNA virus genome. Virus Res 117: 17\u201337.\u00a0\u00a0\u00a0 \u300818. Gorbalenya AE\u3001Enjuanes L\u3001Ziebuhr J\u3001Snijder EJ (2006) Nidovirales\uff1a\u9032\u5316\u6700\u5927\u7684 RNA \u75c5\u6bd2\u57fa\u56e0\u7d44\u3002\u75c5\u6bd2\u7814\u7a76 117\uff1a17-37\u3002\u3009View Article\u3000Google Scholar 19.Snijder EJ, Bredenbeek PJ, Dobbe JC, Thiel V, Ziebuhr J, et al. (2003) Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage. J Mol Biol 331: 991\u20131004.\u00a0\u00a0\u00a0 \u300819. Snijder EJ\u3001Bredenbeek PJ\u3001Dobbe JC\u3001Thiel V\u3001Ziebuhr J \u7b49\u3002 (2003) SARS \u51a0\u72c0\u75c5\u6bd2\u57fa\u56e0\u7d44\u548c\u86cb\u767d\u8cea\u7d44\u7684\u7368\u7279\u548c\u4fdd\u5b88\u7279\u5fb5\uff0c\u5f9e\u51a0\u72c0\u75c5\u6bd2\u7d44 2 \u8b5c\u7cfb\u7684\u65e9\u671f\u5206\u88c2\u3002 J Mol Biol 331\uff1a991-1004\u3002\u3009View Article\u3000Google Scholar 20.Pasternak AO, Spaan WJ, Snijder EJ (2006) Nidovirus transcription: how to make sense\u2026? J Gen Virol 80: 1403\u20131421.\u00a0\u00a0\u00a0 \u300820. Pasternak AO\u3001Spaan WJ\u3001Snijder EJ (2006) Nidovirus \u8f49\u9304\uff1a\u5982\u4f55\u7406\u89e3\u2026\u2026\uff1f J Gen Virol 80\uff1a1403\u20131421\u3002\u3009View Article\u3000Google Scholar 21.Sawicki SG, Sawicki DL, Siddell SG (2007) A Contemporary View of Coronavirus Transcription. J Virol 81: 20\u201329.\u00a0\u00a0\u00a0 \u300821. Sawicki SG\u3001Sawicki DL\u3001Siddell SG\uff082007\uff09\u51a0\u72c0\u75c5\u6bd2\u8f49\u9304\u7684\u7576\u4ee3\u89c0\u9ede\u3002 J Virol 81\uff1a20-29\u3002\u3009View Article\u3000Google Scholar 22.Butterworth BE, Korant BD (1974) Characterization of the large picornaviral polypeptides produced in the presence of zinc ion. J Virol 14: 282\u2013291.\u00a0\u00a0\u00a0 \u300822. Butterworth BE, Korant BD (1974) \u5728\u92c5\u96e2\u5b50\u5b58\u5728\u4e0b\u7522\u751f\u7684\u5927\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u591a\u80bd\u7684\u8868\u5fb5\u3002 J Virol 14\uff1a282-291\u3002\u3009View Article\u3000Google Scholar 23.Denison MR, Perlman S (1986) Translation and processing of mouse hepatitis virus virion RNA in a cell-free system. J Virol 60: 12\u201318.\u00a0\u00a0\u00a0 \u300823. Denison MR, Perlman S (1986) \u5728\u7121\u7d30\u80de\u7cfb\u7d71\u4e2d\u7ffb\u8b6f\u548c\u8655\u7406\u5c0f\u9f20\u809d\u708e\u75c5\u6bd2\u75c5\u6bd2\u9ad4 RNA\u3002 J Virol 60\uff1a12-18\u3002\u3009View Article\u3000Google Scholar 24.Denison MR, Zoltick PW, Hughes SA, Giangreco B, Olson AL, et al. (1992) Intracellular processing of the N-terminal ORF 1a proteins of the coronavirus MHV-A59 requires multiple proteolytic events. Virology 189: 274\u2013284.\u00a0\u00a0\u00a0 \u300824. Denison MR\u3001Zoltick PW\u3001Hughes SA\u3001Giangreco B\u3001Olson AL \u7b49\u3002 (1992) \u51a0\u72c0\u75c5\u6bd2 MHV-A59 \u7684 N \u7aef ORF 1a \u86cb\u767d\u7684\u7d30\u80de\u5167\u52a0\u5de5\u9700\u8981\u591a\u500b\u86cb\u767d\u6c34\u89e3\u4e8b\u4ef6\u3002\u75c5\u6bd2\u5b78 189\uff1a274-284\u3002\u3009View Article\u3000Google Scholar 25.van Hemert MJ, de Wilde AH, Gorbalenya AE, Snijder EJ (2008) The in Vitro RNA Synthesizing Activity of the Isolated Arterivirus Replication\/Transcription Complex Is Dependent on a Host Factor. J Biol Chem 283: 16525\u201316536.\u00a0\u00a0\u00a0 \u300825. van Hemert MJ\u3001de Wilde AH\u3001Gorbalenya AE\u3001Snijder EJ (2008) \u5206\u96e2\u7684\u52d5\u8108\u75c5\u6bd2\u8907\u88fd\/\u8f49\u9304\u8907\u5408\u7269\u7684\u9ad4\u5916 RNA \u5408\u6210\u6d3b\u6027\u53d6\u6c7a\u65bc\u5bbf\u4e3b\u56e0\u5b50\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 283\uff1a16525-16536\u3002\u3009View Article\u3000Google Scholar 26.van Hemert MJ, van den Worm SHE, Knoops K, Mommaas AM, Gorbalenya AE, et al. (2008) SARS-Coronavirus Replication\/Transcription Complexes Are Membrane-Protected and Need a Host Factor for Activity In Vitro. PLoS Pathog 4: e1000054.\u00a0\u00a0\u00a0 \u300826. van Hemert MJ\u3001van den Worm SHE\u3001Knoops K\u3001Mommaas AM\u3001Gorbalenya AE \u7b49\u3002 (2008) SARS-\u51a0\u72c0\u75c5\u6bd2\u8907\u88fd\/\u8f49\u9304\u8907\u5408\u7269\u53d7\u819c\u4fdd\u8b77\uff0c\u9700\u8981\u5bbf\u4e3b\u56e0\u5b50\u624d\u80fd\u9032\u884c\u9ad4\u5916\u6d3b\u52d5\u3002 PLoS \u75c5\u539f\u9ad4 4\uff1ae1000054\u3002\u3009View Article\u3000Google Scholar 27.te Velthuis AJ, Arnold JJ, Cameron CE, van den Worm SH, Snijder EJ (2009) The RNA polymerase activity of SARS-coronavirus nsp12 is primer dependent. Nucleic Acids Res 38: 203\u2013214.\u00a0\u00a0\u00a0 \u300827. te Velthuis AJ\u3001Arnold JJ\u3001Cameron CE\u3001van den Worm SH\u3001Snijder EJ (2009) SARS \u51a0\u72c0\u75c5\u6bd2 nsp12 \u7684 RNA \u805a\u5408\u9176\u6d3b\u6027\u4f9d\u8cf4\u65bc\u5f15\u7269\u3002\u6838\u9178\u7814\u7a76 38\uff1a203-214\u3002\u3009View Article\u3000Google Scholar 28.Beerens N, Selisko B, Ricagno S, Imbert I, van der Zanden L, et al. (2007) De Novo Initiation of RNA Synthesis by the Arterivirus RNA-Dependent RNA Polymerase. J Virol 81: 8384\u20138395.\u00a0\u00a0\u00a0 \u300828. Beerens N\u3001Selisko B\u3001Ricagno S\u3001Imbert I\u3001van der Zanden L \u7b49\u3002 (2007) \u901a\u904e\u52d5\u8108\u75c5\u6bd2 RNA \u4f9d\u8cf4\u7684 RNA \u805a\u5408\u9176\u5f9e\u982d\u958b\u59cb\u5408\u6210 RNA\u3002 J Virol 81\uff1a8384\u20138395\u3002\u3009View Article\u3000Google Scholar 29.van den Born E, Posthuma CC, Knoops K, Snijder EJ (2007) An infectious recombinant equine arteritis virus expressing green fluorescent protein from its replicase gene. J Gen Virol 88: 1196\u20131205.\u00a0\u00a0\u00a0 \u300829. van den Born E, Posthuma CC, Knoops K, Snijder EJ (2007) \u4e00\u7a2e\u50b3\u67d3\u6027\u91cd\u7d44\u99ac\u52d5\u8108\u708e\u75c5\u6bd2\uff0c\u5f9e\u5176\u8907\u5236\u9176\u57fa\u56e0\u8868\u9054\u7da0\u8272\u7192\u5149\u86cb\u767d\u3002 J Gen Virol 88\uff1a1196-1205\u3002\u3009View Article\u3000Google Scholar 30.Sims AC, Burkett SE, Yount B, Pickles RJ (2008) SARS-CoV replication and pathogenesis in an in vitro model of the human conducting airway epithelium. Virus Res 133: 33\u201344.\u00a0\u00a0\u00a0 \u300830. Sims AC\u3001Burkett SE\u3001Yount B\u3001Pickles RJ (2008) SARS-CoV \u5728\u4eba\u985e\u50b3\u5c0e\u6c23\u9053\u4e0a\u76ae\u9ad4\u5916\u6a21\u578b\u4e2d\u7684\u8907\u5236\u548c\u767c\u75c5\u6a5f\u5236\u3002\u75c5\u6bd2\u7814\u7a76 133\uff1a33-44\u3002\u3009View Article\u3000Google Scholar 31.Xu X, Liu Y, Weiss S, Arnold E, Sarafianos SG, et al. (2003) Molecular model of SARS coronavirus polymerase: implications for biochemical functions and drug design. Nucleic Acids Res 31: 7117\u20137130.\u00a0\u00a0\u00a0 \u300831. Xu X\u3001Liu Y\u3001Weiss S\u3001Arnold E\u3001Sarafianos SG \u7b49\u3002 (2003) SARS \u51a0\u72c0\u75c5\u6bd2\u805a\u5408\u9176\u7684\u5206\u5b50\u6a21\u578b\uff1a\u5c0d\u751f\u5316\u529f\u80fd\u548c\u85e5\u7269\u8a2d\u8a08\u7684\u5f71\u97ff\u3002\u6838\u9178\u7814\u7a76 31\uff1a7117-7130\u3002\u3009View Article\u3000Google Scholar 32.Zhai Y, Sun F, Li X, Pang H, Xu X, et al. (2005) Insights into SARS-CoV transcription and replication from the structure of the nsp7-nsp8 hexadecamer. Nat Struct Mol Biol 12: 980\u2013986.\u00a0\u00a0\u00a0 \u300832. \u7fdf\u6bc5\u3001\u5b6b\u98db\u3001\u674e\u65ed\u3001\u9f90\u83ef\u3001\u5f90\u65ed\u7b49\u3002 (2005) \u5f9e nsp7-nsp8 \u5341\u516d\u805a\u9ad4\u7684\u7d50\u69cb\u6d1e\u5bdf SARS-CoV \u8f49\u9304\u548c\u5fa9\u5236\u3002 Nat Struct Mol Biol 12\uff1a980-986\u3002\u3009View Article\u3000Google Scholar 33.Stockman LJ, Bellamy R, Garner P (2006) SARS: Systematic Review of Treatment Effects. PLoS Med 3: e343.\u00a0\u00a0\u00a0 \u300833. Stockman LJ\u3001Bellamy R\u3001Garner P (2006) SARS\uff1a\u6cbb\u7642\u6548\u679c\u7684\u7cfb\u7d71\u8a55\u50f9\u3002 PLoS Med 3: e343\u3002\u3009View Article\u3000Google Scholar 34.Thompson A, Patel K, Tillman H, McHutchison JG (2009) Directly acting antivirals for the treatment of patients with hepatitis C infection: A clinical development update addressing key future challenges. J Hepatol 50: 184\u2013194.\u00a0\u00a0\u00a0 \u300834. Thompson A\u3001Patel K\u3001Tillman H\u3001McHutchison JG (2009) \u7528\u65bc\u6cbb\u7642\u4e19\u578b\u809d\u708e\u611f\u67d3\u60a3\u8005\u7684\u76f4\u63a5\u6297\u75c5\u6bd2\u85e5\u7269\uff1a\u61c9\u5c0d\u672a\u4f86\u95dc\u9375\u6311\u6230\u7684\u81e8\u5e8a\u958b\u767c\u66f4\u65b0\u3002 J Hepatol 50\uff1a184-194\u3002\u3009View Article\u3000Google Scholar 35.De Clercq E (2004) Antivirals and antiviral strategies. Nat Rev Microbiol 2: 704\u2013720.\u00a0\u00a0\u00a0 \u300835. De Clercq E (2004) \u6297\u75c5\u6bd2\u85e5\u7269\u548c\u6297\u75c5\u6bd2\u7b56\u7565\u3002 Nat Rev Microbiol 2: 704\u2013720\u3002\u3009View Article\u3000Google Scholar 36.Thompson AJV, McHutchison JG (2009) Antiviral resistance and specifically targeted therapy for HCV (STAT-C). J Viral Hepat 16: 377\u2013387.\u00a0\u00a0\u00a0 \u300836. Thompson AJV, McHutchison JG (2009) HCV \u6297\u75c5\u6bd2\u8010\u85e5\u6027\u548c\u7279\u7570\u6027\u9776\u5411\u6cbb\u7642 (STAT-C)\u3002 J \u75c5\u6bd2\u6027\u809d\u75c5 16\uff1a377-387\u3002\u3009View Article\u3000Google Scholar 37.Yang W, Lee JY, Nowotny M (2006) Making and Breaking Nucleic Acids: Two-Mg2+-Ion Catalysis and Substrate Specificity. Mol Cell 22: 5\u201313.\u00a0\u00a0\u00a0 \u300837. Yang W\u3001Lee JY\u3001Nowotny M (2006) \u88fd\u9020\u548c\u7834\u58de\u6838\u9178\uff1a\u96d9 Mg2+ \u96e2\u5b50\u50ac\u5316\u548c\u5e95\u7269\u7279\u7570\u6027\u3002\u6469\u723e\u7d30\u80de 22\uff1a5-13\u3002\u3009View Article\u3000Google Scholar 38.Castro C, Smidansky E, Maksimchuk KR, Arnold JJ, Korneeva VS, et al. (2007) Two proton transfers in the transition state for nucleotidyl transfer catalyzed by RNA- and DNA-dependent RNA and DNA polymerases. Proc Natl Acad Sci USA 104: 4267\u20134272.\u00a0\u00a0\u00a0 \u300838. Castro C\u3001Smidansky E\u3001Maksimchuk KR\u3001Arnold JJ\u3001Korneeva VS \u7b49\u3002 (2007) \u7531 RNA \u548c DNA \u4f9d\u8cf4\u6027 RNA \u548c DNA \u805a\u5408\u9176\u50ac\u5316\u7684\u6838\u82f7\u9178\u8f49\u79fb\u7684\u5169\u500b\u904e\u6e21\u614b\u8cea\u5b50\u8f49\u79fb\u3002 Proc Natl Acad Sci USA 104\uff1a4267\u20134272\u3002\u3009View Article\u3000Google Scholar 39.Arnold JJ, Ghosh SK, Cameron CE (1999) Poliovirus RNA-dependent RNA Polymerase (3Dpol). Divalent cation modulation of primer, template and nucleotide selection. J Biol Chem 274: 37060\u201337069.\u00a0\u00a0\u00a0 \u300839. Arnold JJ\u3001Ghosh SK\u3001Cameron CE (1999) \u810a\u9ad3\u7070\u8cea\u708e\u75c5\u6bd2 RNA \u4f9d\u8cf4\u6027 RNA \u805a\u5408\u9176 (3Dpol)\u3002\u5f15\u7269\u3001\u6a21\u677f\u548c\u6838\u82f7\u9178\u9078\u64c7\u7684\u4e8c\u50f9\u967d\u96e2\u5b50\u8abf\u7bc0\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 274\uff1a37060-37069\u3002\u3009View Article\u3000Google Scholar 40.Iuchi S (2001) Three classes of C2H2 zinc finger proteins. Cell Mol Life Sci 58: 625\u2013635.\u00a0\u00a0\u00a0 \u300840. Iuchi S (2001) \u4e09\u985e C2H2 \u92c5\u6307\u86cb\u767d\u3002\u7d30\u80de\u5206\u5b50\u751f\u547d\u79d1\u5b78 58\uff1a625-635\u3002\u3009View Article\u3000Google Scholar 41.Gomis-Ruth XF (2009) Catalytic domain architecture of metzincin metalloproteases. J Biol Chem 284: 15353\u201315357.\u00a0\u00a0\u00a0 \u300841. Gomis-Ruth XF (2009) Metzincin \u91d1\u5c6c\u86cb\u767d\u9176\u7684\u50ac\u5316\u57df\u7d50\u69cb\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 284\uff1a15353\u201315357\u3002\u3009View Article\u3000Google Scholar 42.Yap TL, Xu T, Chen Y-L, Malet H, Egloff M-P, et al. (2007) Crystal Structure of the Dengue Virus RNA-Dependent RNA Polymerase Catalytic Domain at 1.85-Angstrom Resolution. J Virol 81: 4753\u20134765.\u00a0\u00a0\u00a0 \u300842. Yap TL\u3001Xu T\u3001Chen Y-L\u3001Malet H\u3001Egloff M-P \u7b49\u3002 (2007) \u5206\u8fa8\u7387\u70ba 1.85 \u57c3\u7684\u767b\u9769\u71b1\u75c5\u6bd2\u4f9d\u8cf4\u65bc RNA \u7684 RNA \u805a\u5408\u9176\u50ac\u5316\u7d50\u69cb\u57df\u7684\u6676\u9ad4\u7d50\u69cb\u3002 J Virol 81\uff1a4753\u20134765\u3002\u3009View Article\u3000Google Scholar 43.Winek CL, Buehler EV (1966) Intravenous toxicity of zinc pyridinethione and several zinc salts. Toxicol Appl Pharmacol 9: 296\u2013273.\u00a0\u00a0\u00a0 \u300843. Winek CL, Buehler EV (1966) \u5421\u5576\u786b\u916e\u92c5\u548c\u5e7e\u7a2e\u92c5\u9e7d\u7684\u975c\u8108\u6bd2\u6027\u3002 Toxicol Appl Pharmacol 9\uff1a296-273\u3002\u3009View Article\u3000Google Scholar 44.Snyder DR, de Jesus CP, Towfighi J, Jacoby RO, Wedig JH (1979) Neurological, microscopic and enzyme-histochemical assessment of zinc pyrithione toxicity. Food Cosmet Toxicol 17: 651\u2013660.\u00a0\u00a0\u00a0 \u300844. Snyder DR, de Jesus CP, Towfighi J, Jacoby RO, Wedig JH (1979) \u5421\u5576\u786b\u916e\u92c5\u6bd2\u6027\u7684\u795e\u7d93\u5b78\u3001\u986f\u5fae\u93e1\u548c\u9176\u7d44\u7e54\u5316\u5b78\u8a55\u4f30\u3002\u98df\u54c1\u5316\u599d\u54c1\u6bd2\u7406\u5b78 17\uff1a651-660\u3002\u3009View Article\u3000Google Scholar 45.Magda D, Lecane P, Wang Z, Hu W, Thiemann P, et al. (2008) Synthesis and anticancer properties of water-soluble zinc ionophores. Cancer Res 68: 5318\u20135325.\u00a0\u00a0\u00a0 \u300845. Magda D\u3001Lecane P\u3001Wang Z\u3001Hu W\u3001Thiemann P \u7b49\u3002 (2008) \u6c34\u6eb6\u6027\u92c5\u96e2\u5b50\u8f09\u9ad4\u7684\u5408\u6210\u548c\u6297\u764c\u7279\u6027\u3002\u764c\u75c7\u7814\u7a76 68\uff1a5318-5325\u3002\u3009View Article\u3000oogle Scholar 46.Snijder EJ, van der Meer Y, Zevenhoven-Dobbe J, Onderwater JJM, van der Meulen J, et al. (2006) Ultrastructure and Origin of Membrane Vesicles Associated with the Severe Acute Respiratory Syndrome Coronavirus Replication Complex. J Virol 80: 5927\u20135940.\u00a0\u00a0\u00a0 \u300846. Snijder EJ\u3001van der Meer Y\u3001Zevenhoven-Dobbe J\u3001Onderwater JJM\u3001van der Meulen J \u7b49\u3002 (2006) \u8207\u56b4\u91cd\u6025\u6027\u547c\u5438\u7cfb\u7d71\u7d9c\u5408\u75c7\u51a0\u72c0\u75c5\u6bd2\u8907\u88fd\u8907\u5408\u9ad4\u76f8\u95dc\u7684\u819c\u56ca\u6ce1\u7684\u8d85\u5fae\u7d50\u69cb\u548c\u8d77\u6e90\u3002 J Virol 80\uff1a5927-5940\u3002\u3009View Article\u3000Google Scholar 47.Studier F (2005) Protein production by auto-induction in high density shaking cultures. Protein Expr Purif 41: 207\u2013234.\u00a0\u00a0\u00a0 \u300847. Studier F (2005) \u5728\u9ad8\u5bc6\u5ea6\u632f\u76ea\u57f9\u990a\u4e2d\u901a\u904e\u81ea\u52d5\u8a98\u5c0e\u7522\u751f\u86cb\u767d\u8cea\u3002\u86cb\u767d\u8cea Expr Purif 41\uff1a207-234\u3002\u3009View Article\u3000Google Scholar   \u91cd\u5fb7\u4e26\u4e14\u5c07\u5728\u5317\u4eac\u7684\u300c\u4e2d\u570b\u79d1\u5b78\u9662\u751f\u7269\u7269\u7406\u7814\u7a76\u6240\u611f\u67d3\u8207\u514d\u75ab\u5b78\u91cd\u9ede\u5be6\u9a57\u5ba4\u300d\u5728\u91ab\u5b78\u96dc\u8a8c\u4e0a\u6240\u767c\u8868\u7684\u4e00\u7bc7\u82f1\u6587\u7814\u7a76\u5831\u544a\u300a\u92c5\u6307\u6297\u75c5\u6bd2\u86cb\u767d\u6291\u5236\u7532\u578b\u6d41\u611f\u75c5\u6bd2\u86cb\u767d\u8868\u9054\u4ee5\u53ca\u88ab\u75c5\u6bd2\u7de8\u78bcNS1 \u5c0d\u6297\u7684\u7c21\u8981\u5f62\u5f0f\u300b\u7684\u4e2d\u6587\u81ea\u8b6f\u7248\u4e5f\u5bc4\u4e0a\u7d66\u4ed6\u53c3\u8003(\u5982\u9644\u6a94)\u3002 \u4e26\u4e14\uff0c\u91cd\u5fb7\u9084\u767c\u53bb\u4ee5\u4e0b\u5716\u7247\u8207\u8aaa\u660e\uff1a   \u300e\u9019\u5c31\u662f\u92c5\u6307\u7d50\u69cb\u5716\uff0c\u7531\u65bc\u9019\u662f\u87af\u5408\u7d50\u69cb\uff0c\u56e0\u6b64\u5b83\u80fd\u5f88\u5bb9\u6613\u5c07\u92c5\u96e2\u5b50\u7a7f\u900f\u7d30\u80de\u58c1\u800c\u8207\u8eb2\u85cf\u5728\u7d30\u80de\u5167\u840c\u767c\u7684\u75c5\u6bd2\u56e0\u70ba\u767c\u751f\u6291\u5236\u7ffb\u8b6f\u548c\u4fc3\u9032\u75c5\u6bd2 mRNA\u7684\u964d\u89e3\uff0c\u56e0\u6b64\u901a\u904e PARP \u7d50\u69cb\u57df\u800c\u4fc3\u9032 IAV \u86cb\u767d\u964d\u89e3\uff0c\u5be6\u9a57\u5df2\u7d93\u8b49\u5be6\u5b83\u53ef\u8b93\u5165\u4fb5\u5230\u7d30\u80de\u5167\u7684\u75c5\u6bd2\u4e4bRNA\u6a5f\u8f49\u767c\u751f\u4f11\u514b(\u4f46\u4e0d\u4e00\u5b9a\u6703\u6b7b\u4ea1)\uff0c\u800c\u4f7f\u5f97\u539f\u672c\u7121\u751f\u547d\u7684\u75c5\u6bd2\u56e0\u6b64\u4e0d\u80fd\u5927\u91cf\u8907\u88fd\u840c\u751f\uff0c\u800c\u53ef\u4ee5\u70ba\u4eba\u9ad4\u7684\u514d\u75ab\u7cfb\u7d71\u89e3\u6c7a\u3002\u300f \u9019\u4e00\u4f4d\u738b\u59d3\u7db2\u53cb\u53c8\u767c\u4f86\u4ee5\u4e0b\u4fe1\u606f\uff1a\u300e\u80fd\u5920\u6bba\u75c5\u6bd2\u800c\u4e0d\u6703\u6bba\u6b7b\u6211\u5011\u81ea\u5df1\u7684\u7d30\u80de,\u5929\u4e0b\u6709\u9019\u9ebc\u597d\u7684\u4e8b\u55ce\uff1f\u4e0d\u904e\u662f\u71df\u990a\u88dc\u52a9\u54c1\u800c\u5df2\uff01\u300f \u91cd\u5fb7\u770b\u4ed6\u5728\u610f\u6c23\u7528\u4e8b\uff0c\u56e0\u6b64\u5c31\u56de\u8986\u4ed6\u8aaa\uff1a\u300e\u96a8\u60a8\u9ad8\u8208\u600e\u9ebc\u8aaa\u90fd\u597d\uff0c\u6211\u6c92\u6709\u8aaa\u5b83\u662f\u85e5\uff0c\u53ea\u8aaa\u662f\u4fdd\u5065\u54c1\u800c\u5df2\u3002\u300f\u767c\u5b8c\u4e86\u9019\u4fe1\u606f\u4e4b\u5f8c\uff0c\u91cd\u5fb7\u767c\u8a93\u4ee5\u5f8c\u518d\u4e5f\u4e0d\u5c0d\u9019\u7a2e\u96de\u86cb\u88e1\u6311\u9aa8\u982d\u7684\u4eba\u63d0\u4f9b\u4efb\u4f55\u6709\u50f9\u503c\u7684\u8cc7\u8a0a\u4e86\u3002 \u4f46\u662f\uff0c\u91cd\u5fb7\u9084\u662f\u8981\u544a\u8a34\u5927\u5bb6\u70ba\u4f55\u8981\u5efa\u8b70\u5927\u5bb6\u5403\u542b\u92c5\u96e2\u5b50\u7684\u4fdd\u5065\u98df\u54c1\uff0c\u7576\u7136\u92c5\u5f9e\u98df\u7269\u4e2d\u651d\u53d6\u4e5f\u6709\uff0c\u4f46\u662f\u98df\u7269\u5fc5\u9808\u7d93\u904e\u6d88\u5316\u5f8c\u92c5\u96e2\u5b50\u624d\u6703\u900f\u904e\u8840\u6db2\u800c\u5230\u4eba\u9ad4\u5668\u5b98\uff0c\u9019\u500b\u904e\u7a0b\u592a\u9577\u592a\u4e45\u4e86\uff0c\u56e0\u6b64\u92c5\u96e2\u5b50\u53ef\u80fd\u53c8\u8207\u5176\u4ed6\u7269\u8cea\u7d50\u5408\u6210\u5316\u5408\u7269\uff0c\u800c\u518d\u4e0d\u80fd\u767c\u751f\u8207\u8ca0\u96e2\u5b50\u7684\u75c5\u6bd2\u4e2d\u548c\u7684\u6548\u679c\u3002\u56e0\u6b64\u5c31\u66f4\u6c92\u6709\u767c\u63ee\u5230\u92c5\u96e2\u5b50\u6240\u7368\u5177\u7684\u87af\u5408\u4ea4\u63db\u6548\u679c\u3002 \u7531\u65bc\uff0c\u611f\u5192\u6216\u662fCOVID-19\u7684\u521d\u671f\u75c5\u72c0\u4e3b\u8981\u90fd\u662f\u91dd\u5c0d\u4e0a\u652f\u6c23\u7ba1\uff0c\u56e0\u6b64\u4ee3\u8868\u547c\u5438\u7ba1\u9053\u7684\u75c5\u6bd2\u91cf\u6700\u5927\uff0c\u56e0\u6b64\u53ef\u4ee5\u542b\u5728\u5634\u4e2d\u6162\u6162\u5206\u89e3\u7684\u5589\u7cd6\u4e4b\u6548\u679c\u6700\u597d\uff0c\u56e0\u70ba\u5b83\u8b93\u901a\u904e\u7684\u6c23\u6d41\u4e2d\u7684\u75c5\u6bd2\u5148\u88ab\u92c5\u96e2\u5b50\u4e2d\u548c\uff0c\u800c\u7cd6\u6c41\u6d41\u5165\u98df\u9053\u6642\u92c5\u96e2\u5b50\u4e5f\u6703\u6ea2\u5165\u4e0a\u652f\u6c23\u7ba1\u3002\u9019\u5c31\u662f\u70ba\u4f55\u91cd\u5fb7\u9996\u5148\u63a8\u85a6\u5df2\u7d93\u5728\u7f8e\u570b\u98a8\u884c30\u591a\u5e74\u7684\u611f\u5192\u9810\u9632\u7528\u85e5Cold Eeze\u4e2d\u6587\u8b6f\u540d\u70ba\u3010\u92c5\u7cd6\u3011\uff0c\u56e0\u70ba\u5b83\u5df2\u7d93\u904e\u4e86\u5c08\u5229\u4fdd\u8b77\u671f\uff0c\u5b78\u540d\u85e5\u540d\u662fGeneric Version\uff0c\u56e0\u70ba\u552e\u50f9\u8f03\u4fbf\u5b9c(\u4f46\u662f\u73fe\u5728\u5168\u7403\u6436\u8cfc\u5f88\u53ef\u80fd\u7f3a\u8ca8)\u3002 \u73fe\u5728\uff0c\u91cd\u5fb7\u8981\u544a\u8a34\u5927\u5bb6\u4e00\u500b\u79d8\u5bc6\uff0c\u90a3\u5c31\u662f\u70ba\u4f55\u91cd\u5fb7\u8981\u5c0d\u92c5\u96e2\u5b50\u9032\u884c\u7814\u7a76\u5462\uff0c\u6545\u4e8b\u662f\u9019\u6a23\u5b50\u7684\uff0c\u7531\u65bc\u4e00\u5e74\u524d \u91cd\u5fb7 \u8acb\u793a\u201c\u9748\u7236\u201d(\u5143\u59cb\u5929\u5c0a)\u8aaa\uff1a\u300e\u65e2\u7136\u00a0 \u9748\u7236\u80fd\u63d0\u524d\u534a\u5e74\u8b66\u544a\u6211\u5011\u5c07\u6709\u6b64\u5927\u75ab\u60c5\uff0c\u662f\u5426\u80fd\u544a\u77e5\u5f92\u5152\u5982\u4f55\u88fd\u5f97\u89e3\u85e5\uff1f\u300f\u64f2\u832d\u61c9\u8a31\u5f8c\uff0c\u91cd\u5fb7 \u6b63\u4e0d\u77e5\u5982\u4f55\u518d\u554f\u9019\u89e3\u85e5\u79d8\u65b9\u6642\uff0c\u8166\u4e2d\u7a81\u7136\u6e67\u73fe\u4e00\u500b\u201c\u92c5\u201d\u5b57\uff0c\u64f2\u832d\u518d\u554f\u78ba\u5b9a\u795e\u8aed\u5c31\u662f\u6b64\u201c\u92c5\u201d\u5b57\u7121\u8aa4\u3002 \u5728\u6b64\u4e4b\u5f8c\uff0c\u91cd\u5fb7\u624d\u958b\u59cb\u8a73\u95b1\u5404\u7a2e\u82f1\u6587\u91ab\u5b78\u6587\u737b\uff0c\u8d8a\u767c\u8b49\u5be6\u92c5\u96e2\u5b50\u6709\u6291\u5236mRNA\u75c5\u6bd2\u7684\u6548\u679c\uff0c\u800c\u4e14\u56e0\u70ba\u92c5\u96e2\u5b50\u662f\u63a1\u53d6\u87af\u5408\u65b9\u5f0f\u8b93\u92c5\u96e2\u5b50\u7a7f\u900f\u7d30\u80de\u58c1\u9032\u5165\u4f86\u964d\u89e3\u75c5\u6bd2\u86cb\u767d\u8907\u88fd\u6240\u9700\u6c42\u7684PARP \u7d50\u69cb\u57df\uff0c\u56e0\u6b64\u9019\u662f\u4e00\u7a2e\u7cbe\u6e96\u7834\u7a97\u5f0f\u7684\u65ac\u9996\u884c\u52d5\uff0c\u5c0d\u65bc\u7d30\u80de\u7684\u50b7\u5bb3\u751a\u5c0f\uff0c\u56e0\u6b64\u5c0d\u65bc\u4eba\u9ad4\u7684\u5371\u5bb3\u751a\u4f4e\u3002 \u91cd\u5fb7\u4e00\u5e74\u524d\u5c31\u628a\u7814\u7a76\u6210\u679c\u516c\u958b\uff0c\u4e00\u4f86\u56e0\u70ba\u81ea\u5df1\u962e\u56ca\u7f9e\u6f80\uff0c\u7121\u6cd5\u7e7c\u7e8c\u7814\u767c\uff0c\u4e8c\u4f86\u91cd\u5fb7\u5df2\u7d93\u9810\u7aba\u5230\u672a\u4f86\u9084\u6709\u5176\u4ed6\u56b4\u91cd\u75be\u75c5\uff0c\u800c\u4e14COVID-19\u9084\u6703\u5927\u7a81\u8b8a\uff0c\u56e0\u6b64\u8b93\u5176\u4ed6\u4eba\u63a5\u68d2\u4f86\u7814\u7a76\u6216\u8a31\u6703\u66f4\u6709\u6210\u6548\u3002\" \/>\n<meta property=\"og:url\" content=\"https:\/\/pingtungtimes.com.tw\/?p=104514\" \/>\n<meta property=\"og:site_name\" content=\"\u5c4f\u6771\u6642\u5831\u96fb\u5b50\u5831\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/pingtungnews\" \/>\n<meta property=\"article:published_time\" content=\"2022-05-27T15:08:21+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/pingtungtimes.com.tw\/wp-content\/uploads\/2022\/05\/\u4e00\u822c\u4eba\u5f9e\u98df\u7269\u4e2d\u651d\u53d6\u92c5\u70ba\u7684\u5c31\u662f\u58ef\u967d\u8207\u589e\u9aee.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"897\" \/>\n\t<meta property=\"og:image:height\" content=\"468\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"fanjiang\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"\u4f5c\u8005:\" \/>\n\t<meta name=\"twitter:data1\" content=\"fanjiang\" \/>\n\t<meta name=\"twitter:label2\" content=\"\u9810\u4f30\u95b1\u8b80\u6642\u9593\" \/>\n\t<meta name=\"twitter:data2\" content=\"9 \u5206\u9418\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"NewsArticle\",\"@id\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/?p=104514#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/?p=104514\"},\"author\":{\"name\":\"fanjiang\",\"@id\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/#\\\/schema\\\/person\\\/90cf880d4d210d8db447ae0199435aa4\"},\"headline\":\"\u597d\u201c\u92c5\u201d\u3000\u4e5f\u6703\u60f9\u4e86\u4e00\u8eab\u8165\",\"datePublished\":\"2022-05-27T15:08:21+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/?p=104514\"},\"wordCount\":1787,\"commentCount\":0,\"publisher\":{\"@id\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/?p=104514#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/wp-content\\\/uploads\\\/2022\\\/05\\\/\u4e00\u822c\u4eba\u5f9e\u98df\u7269\u4e2d\u651d\u53d6\u92c5\u70ba\u7684\u5c31\u662f\u58ef\u967d\u8207\u589e\u9aee.jpg\",\"keywords\":[\"\u674e\u91cd\u5fb7\",\"\u78ba\u8a3a\u7652\u5f8c\u8005\",\"\u88dc\u5145\u92c5\u96e2\u5b50\u98df\u54c1\"],\"articleSection\":[\"\u9577\u7167\u5c08\u6b04\"],\"inLanguage\":\"zh-TW\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/pingtungtimes.com.tw\\\/?p=104514#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/?p=104514\",\"url\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/?p=104514\",\"name\":\"\u597d\u201c\u92c5\u201d\u3000\u4e5f\u6703\u60f9\u4e86\u4e00\u8eab\u8165 - \u5c4f\u6771\u6642\u5831\u96fb\u5b50\u5831\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/?p=104514#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/?p=104514#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/wp-content\\\/uploads\\\/2022\\\/05\\\/\u4e00\u822c\u4eba\u5f9e\u98df\u7269\u4e2d\u651d\u53d6\u92c5\u70ba\u7684\u5c31\u662f\u58ef\u967d\u8207\u589e\u9aee.jpg\",\"datePublished\":\"2022-05-27T15:08:21+00:00\",\"description\":\"\u3010\u674e\u91cd\u5fb7\uff0f\u5c08\u6b04\u5831\u5c0e2022\\\/05\\\/27\u3011\u3000\u4eca\u65e9\uff0c\u6709\u4e00\u78ba\u8a3a\u7652\u5f8c\u8005\u8207\u91cd\u5fb7\u8a0e\u8ad6\u4ed6\u7684\u75c5\u60c5\uff0c\u91cd\u5fb7\u5efa\u8b70\u4ed6\u88dc\u5145\u92c5\u96e2\u5b50\u98df\u54c1\uff0c\u4e26\u5c07\u6b64\u4e00\u4fe1\u606f\u4e5f\u767c\u7d66\u5927\u5bb6\u77e5\u66c9\uff1a \u300e\u6709\u78ba\u8a3a\u7652\u5f8c\u8005\u554f\u91cd\u5fb7\u8981\u5982\u4f55\u4fdd\u5065\u4ee5\u9632\u5fa9\u967d\uff0c\u91cd\u5fb7 \u7686\u5efa\u8b70\u4ed6\u5403\u3010\u92c5\u7cd6\u3011(\u985e\u4f3c\u5589\u7247)\u5c0f\u5b69\u5247\u5403\u3010\u5152\u7ae5\u92c5\u8edf\u7cd6\u3011\uff0c\u6216\u662f\u3010\u9175\u6bcd\u92c5\u3011\u3001\u3010\u87af\u5408\u92c5\u3011\u6216\u662f\u3010\u8461\u8404\u7cd6\u9178\u92c5\u3011\u4f86\u6d88\u6ec5\u6f5b\u85cf\u4eba\u9ad4\u5c1a\u672a\u767c\u4f5c\u7684\u75c5\u6bd2\u3002\u300f \u5f8c\u4f86\u5c31\u63a5\u7372\u4e00\u4f4d\u738b\u59d3\u7db2\u53cb\u56de\u8986\u8aaa\uff1a\u300e\u4e0d\u5c0d\u5594\uff01\u92c5,\u4eba\u985e\u5b58\u6d3b\u6240\u9700\u8981\u7684\u5143\u7d20\u4e4b\u4e00,\u5f9e\u5929\u7136\u98df\u7269\u76f4\u63a5\u651d\u53d6\u5c31\u8db3\u5920\u4e86,\u4eba\u70ba\u8d85\u91cf\u7684\u88dc\u5145\u6703\u51fa\u554f\u984c\u7684\uff01\u92c5,\u4e0d\u6703\u76f4\u63a5\u6bba\u75c5\u6bd2\u7684\uff01\u300f \u9019\u4e00\u4f4d\u738b\u5148\u751f\uff0c\u5927\u6982\u6f0f\u770b\u4e86\u91cd\u5fb7\u5728\u4e00\u5e74\u524d\u5728\u672c\u6703\u7fa4\u7d44\u4e0a\u767c\u8868\u7684\u5e7e\u7bc7\u4ee5\u92c5\u96e2\u5b50\u4f86\u6d88\u6ec5\u75c5\u6bd2\u7684\u6587\u7ae0\uff0c\u56e0\u6b64\uff0c\u91cd\u5fb7\u5c31\u56de\u8986\u4ed6\u8aaa\uff1a\u300e\u6211\u770b\u904e\u4e86\u8a31\u591a\u82f1\u6587\u91ab\u5b78\u6587\u737b\uff0c\u4e5f\u8207\u9ec3\u25cb\u5bcc\u535a\u58eb\u548c\u9673\u25cb\u5e73\u91ab\u5e2b\u8a0e\u8ad6\u904e\u3002\u60a8\u5982\u679c\u60f3\u8981\u770b\u9019\u4e00\u4e9b\u82f1\u6587\u91ab\u5b78\u6587\u737b\uff0c\u6211\u53ef\u4ee5\u5bc4\u53bb\u7d66\u60a8\u3002\u300f\u7136\u5f8c \u91cd\u5fb7 \u5c31\u5c31\u5bc4\u53bb\u7d66\u4ed6\u4ee5\u4e0b\u7684\u8cc7\u6599\uff1a References\u53c3\u8003\u6587\u737b 1.Lazarczyk M, Favre M (2008) Role of Zn2+ ions in host-virus interactions. J Virol 82: 11486\u201311494.\u00a0\u00a0\u00a0 \u30081. Lazarczyk M, Favre M (2008) Zn2+ \u96e2\u5b50\u5728\u5bbf\u4e3b-\u75c5\u6bd2\u76f8\u4e92\u4f5c\u7528\u4e2d\u7684\u4f5c\u7528\u3002 J Virol 82\uff1a11486\u201311494\u3002\u3009\u3000View Article\u3000Google Scholar 2.Frederickson CJ, Koh JY, Bush AI (2005) Neurobiology of zinc in health and disease. Nat Rev Neurosci 6: 449\u2013462.\u00a0\u00a0\u00a0 \u30082. Frederickson CJ\u3001Koh JY\u3001Bush AI (2005) \u92c5\u5728\u5065\u5eb7\u548c\u75be\u75c5\u4e2d\u7684\u795e\u7d93\u751f\u7269\u5b78\u3002 Nat Rev Neurosci 6\uff1a449-462\u3002\u3009View Article\u3000Google Scholar 3.Alirezaei M, Nairn AC, Glowinski J, Premont J, Marin P (1999) Zinc inhibits protein synthesis in neurons: potential rol of phosphorylation of translation initiation factor-2a. J Biol Chem 274: 32433\u201332438.\u00a0\u00a0\u00a0 \u30083. Alirezaei M\u3001Nairn AC\u3001Glowinski J\u3001Premont J\u3001Marin P (1999) \u92c5\u6291\u5236\u795e\u7d93\u5143\u4e2d\u7684\u86cb\u767d\u8cea\u5408\u6210\uff1a\u7ffb\u8b6f\u8d77\u59cb\u56e0\u5b50 2a \u78f7\u9178\u5316\u7684\u6f5b\u5728\u4f5c\u7528\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 274\uff1a32433\u201332438\u3002\u3009View Article\u3000Google Scholar 4.Uchide N, Ohyama K, Bessho T, Yuan B, Yamakawa T (2002) Effect of antioxidants on apoptosis induced by influenza virus infection: inhibition of viral gene replication and transcription with pyrrolidine dithiocarbamate. Antiviral Res 56: 207\u2013217.\u00a0\u00a0\u00a0 \u30084. Uchide N, Ohyama K, Bessho T, Yuan B, Yamakawa T (2002) \u6297\u6c27\u5316\u5291\u5c0d\u6d41\u611f\u75c5\u6bd2\u611f\u67d3\u8a98\u5c0e\u7684\u7d30\u80de\u51cb\u4ea1\u7684\u5f71\u97ff\uff1a\u7528\u5421\u54af\u70f7\u4e8c\u786b\u4ee3\u6c28\u57fa\u7532\u9178\u916f\u6291\u5236\u75c5\u6bd2\u57fa\u56e0\u8907\u5236\u548c\u8f49\u9304\u3002\u6297\u75c5\u6bd2\u7814\u7a76 56\uff1a207-217\u3002\u3009View Article\u3000Google Scholar 5.Suara RO, Crowe JEJ (2004) Effect of zinc salts on respiratory syncytial virus replication. Antimicrob Agents Chemother 48: 783\u2013790.\u00a0\u00a0\u00a0 \u30085. Suara RO, Crowe JEJ (2004) \u92c5\u9e7d\u5c0d\u547c\u5438\u9053\u5408\u80de\u75c5\u6bd2\u8907\u88fd\u7684\u5f71\u97ff\u3002\u6297\u5fae\u751f\u7269\u5291 Chemother 48\uff1a783-790\u3002\u3009View Article\u3000Google Scholar 6.Gaudernak E, Seipelt J, Triendl A, Grassauer A, Kuechler E (2002) Antiviral Effects of Pyrrolidine Dithiocarbamate on Human Rhinoviruses. J Virol 76: 6004\u20136015.\u00a0\u00a0\u00a0 \u30086. Gaudernak E\u3001Seipelt J\u3001Triendl A\u3001Grassauer A\u3001Kuechler E (2002) \u5421\u54af\u70f7\u4e8c\u786b\u4ee3\u6c28\u57fa\u7532\u9178\u916f\u5c0d\u4eba\u9f3b\u75c5\u6bd2\u7684\u6297\u75c5\u6bd2\u4f5c\u7528\u3002 J Virol 76\uff1a6004-6015\u3002\u3009View Article\u3000Google Scholar 7.Si X, McManus BM, Zhang J, Yuan J, Cheung C, et al. (2005) Pyrrolidine Dithiocarbamate Reduces Coxsackievirus B3 Replication through Inhibition of the Ubiquitin-Proteasome Pathway. J Virol 79: 8014\u20138023.\u00a0\u00a0\u00a0 \u30087. Si X, McManus BM, Zhang J, Yuan J, Cheung C, et al. (2005) Pyrrolidine Dithiocarbamate \u901a\u904e\u6291\u5236\u6cdb\u7d20-\u86cb\u767d\u9176\u9ad4\u9014\u5f91\u6e1b\u5c11\u67ef\u85a9\u5947\u75c5\u6bd2 B3 \u8907\u88fd\u3002 J Virol 79\uff1a8014-8023\u3002\u3009View Article\u3000Google Scholar 8.Korant BD, Kauer JC, Butterworth BE (1974) Zinc ions inhibit replication of rhinoviruses. Nature 248: 588\u2013590.\u00a0\u00a0\u00a0 \u30088. Korant BD, Kauer JC, Butterworth BE (1974) \u92c5\u96e2\u5b50\u6291\u5236\u9f3b\u75c5\u6bd2\u7684\u8907\u5236\u3002\u81ea\u7136 248\uff1a588-590\u3002\u3009View Article\u3000Google Scholar 9.Polatnick J, Bachrach HL (1978) Effect of zinc and other chemical agents on foot-and-mouth-disease virus replication. Antimicrob Agents Chemother 13: 731\u2013734.\u00a0\u00a0 \u00a0\u30089. Polatnick J, Bachrach HL (1978) \u92c5\u548c\u5176\u4ed6\u5316\u5b78\u8a66\u5291\u5c0d\u53e3\u8e44\u75ab\u75c5\u6bd2\u8907\u88fd\u7684\u5f71\u97ff\u3002\u6297\u5fae\u751f\u7269\u5291 Chemother 13\uff1a731-734\u3002\u3009View Article\u3000Google Scholar 10.Lanke K, Krenn BM, Melchers WJG, Seipelt J, van Kuppeveld FJM (2007) PDTC inhibits picornavirus polyprotein processing and RNA replication by transporting zinc ions into cells. J Gen Virol 88: 1206\u20131217.\u00a0\u00a0\u00a0 \u300810. Lanke K\u3001Krenn BM\u3001Melchers WJG\u3001Seipelt J\u3001van Kuppeveld FJM (2007) PDTC \u901a\u904e\u5c07\u92c5\u96e2\u5b50\u8f49\u904b\u5230\u7d30\u80de\u4e2d\u4f86\u6291\u5236\u5c0f\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u591a\u86cb\u767d\u52a0\u5de5\u548c RNA \u8907\u88fd\u3002 J Gen Virol 88\uff1a1206-1217\u3002\u3009View Article\u3000Google Scholar7 11.Krenn BM, Gaudernak E, Holzer B, Lanke K, Van Kuppeveld FJM, et al. (2009) Antiviral Activity of the Zinc Ionophores Pyrithione and Hinokitiol against Picornavirus Infections. J Virol 83: 58\u201364.\u00a0\u00a0\u00a0 \u300811. Krenn BM\u3001Gaudernak E\u3001Holzer B\u3001Lanke K\u3001Van Kuppeveld FJM \u7b49\u3002 (2009) \u92c5\u96e2\u5b50\u8f09\u9ad4\u5421\u5576\u786b\u916e\u548c\u6a9c\u9187\u5c0d\u5c0f\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u611f\u67d3\u7684\u6297\u75c5\u6bd2\u6d3b\u6027\u3002 J Virol 83\uff1a58-64\u3002\u3009View Article\u3000Google Scholar 12.Zalewski PD, Forbes IJ, Betts WH (1993) Correlation of apoptosis with change in intracellular labile Zn(II) using Zinquin , a new specific fluorescent probe for Zn(II). Biochem J 296: 403\u2013408.\u00a0 \u300812. Zalewski PD, Forbes IJ, Betts WH (1993) \u4f7f\u7528 Zinquin \uff0c\u7d30\u80de\u51cb\u4ea1\u8207\u7d30\u80de\u5167\u4e0d\u7a69\u5b9a\u92c5\uff08II\uff09\u8b8a\u5316\u7684\u76f8\u95dc\u6027\uff0c\u4e00\u7a2e\u65b0\u7684\u7279\u7570\u6027Zn(II) \u7684\u7192\u5149\u63a2\u91dd\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 296\uff1a403-408\u3002\u3009View Article\u3000Google Scholar 13.Baum EZ, Bebernitz GA, Palant O, Mueller T, Plotch SJ (1991) Purification, properties, and mutagenesis of poliovirus 3C protease. Virology 165: 140\u2013150.\u00a0\u00a0\u00a0 \u300813. Baum EZ\u3001Bebernitz GA\u3001Palant O\u3001Mueller T\u3001Plotch SJ (1991) \u810a\u9ad3\u7070\u8cea\u708e\u75c5\u6bd2 3C \u86cb\u767d\u9176\u7684\u7d14\u5316\u3001\u7279\u6027\u548c\u8a98\u8b8a\u3002\u75c5\u6bd2\u5b78 165\uff1a140-150\u3002\u3009View Article\u3000Google Scholar 14.Cordingley MG, Register RB, Callahan PL, Garsky VM, Colonno RJ (1989) Cleavage of small peptides in vitro by human rhinovirus 14 3C protease expressed in Escherichia coli. J Virol 63: 5037\u20135045.\u00a0\u00a0\u00a0 \u300814. Cordingley MG\u3001Register RB\u3001Callahan PL\u3001Garsky VM\u3001Colonno RJ (1989) \u5728\u5927\u8178\u687f\u83cc\u4e2d\u8868\u9054\u7684\u4eba\u9f3b\u75c5\u6bd2 14 3C \u86cb\u767d\u9176\u9ad4\u5916\u88c2\u89e3\u5c0f\u80bd\u3002 J Virol 63\uff1a5037-5045\u3002\u3009View Article\u3000Google Scholar 15.Ferrari E, Wright-Minogue J, Fang JW, Baroudy BM, Lau JY, et al. (1999) Characterization of soluble hepatitis C virus RNA-dependent RNA polymerase expressed in Escherichia coli. J Virol 73: 1649\u20131654.\u00a0\u00a0\u00a0 \u300815. Ferrari E\u3001Wright-Minogue J\u3001Fang JW\u3001Baroudy BM\u3001Lau JY \u7b49\u3002 (1999) \u5728\u5927\u8178\u687f\u83cc\u4e2d\u8868\u9054\u7684\u53ef\u6eb6\u6027\u4e19\u578b\u809d\u708e\u75c5\u6bd2 RNA \u4f9d\u8cf4\u6027 RNA \u805a\u5408\u9176\u7684\u8868\u5fb5\u3002 J Virol 73\uff1a1649-1654\u3002\u3009View Article\u3000Google Scholar 16.Hung M, Gibbs CS, Tsiang M (2002) Biochemical characterization of rhinovirus RNA-dependent RNA polymerase. Antiviral Res 56: 99\u2013114.\u00a0\u00a0\u00a0 \u300816. Hung M\u3001Gibbs CS\u3001Tsiang M (2002) \u9f3b\u75c5\u6bd2 RNA \u4f9d\u8cf4\u6027 RNA \u805a\u5408\u9176\u7684\u751f\u5316\u7279\u5fb5\u3002\u6297\u75c5\u6bd2\u7814\u7a76 56\uff1a99-114\u3002\u3009View Article\u3000Google Scholar 17.Perlman S, Netland J (2009) Coronaviruses post-SARS: update on replication and pathogenesis. Nat Rev Micro 7: 439\u2013450.\u00a0\u00a0\u00a0 \u300817. Perlman S, Netland J (2009) SARS \u5f8c\u7684\u51a0\u72c0\u75c5\u6bd2\uff1a\u8907\u88fd\u548c\u767c\u75c5\u6a5f\u5236\u7684\u66f4\u65b0\u3002 Nat Rev Micro 7\uff1a439-450\u3002\u3009View Article\u3000Google Scholar 18.Gorbalenya AE, Enjuanes L, Ziebuhr J, Snijder EJ (2006) Nidovirales: evolving the largest RNA virus genome. Virus Res 117: 17\u201337.\u00a0\u00a0\u00a0 \u300818. Gorbalenya AE\u3001Enjuanes L\u3001Ziebuhr J\u3001Snijder EJ (2006) Nidovirales\uff1a\u9032\u5316\u6700\u5927\u7684 RNA \u75c5\u6bd2\u57fa\u56e0\u7d44\u3002\u75c5\u6bd2\u7814\u7a76 117\uff1a17-37\u3002\u3009View Article\u3000Google Scholar 19.Snijder EJ, Bredenbeek PJ, Dobbe JC, Thiel V, Ziebuhr J, et al. (2003) Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage. J Mol Biol 331: 991\u20131004.\u00a0\u00a0\u00a0 \u300819. Snijder EJ\u3001Bredenbeek PJ\u3001Dobbe JC\u3001Thiel V\u3001Ziebuhr J \u7b49\u3002 (2003) SARS \u51a0\u72c0\u75c5\u6bd2\u57fa\u56e0\u7d44\u548c\u86cb\u767d\u8cea\u7d44\u7684\u7368\u7279\u548c\u4fdd\u5b88\u7279\u5fb5\uff0c\u5f9e\u51a0\u72c0\u75c5\u6bd2\u7d44 2 \u8b5c\u7cfb\u7684\u65e9\u671f\u5206\u88c2\u3002 J Mol Biol 331\uff1a991-1004\u3002\u3009View Article\u3000Google Scholar 20.Pasternak AO, Spaan WJ, Snijder EJ (2006) Nidovirus transcription: how to make sense\u2026? J Gen Virol 80: 1403\u20131421.\u00a0\u00a0\u00a0 \u300820. Pasternak AO\u3001Spaan WJ\u3001Snijder EJ (2006) Nidovirus \u8f49\u9304\uff1a\u5982\u4f55\u7406\u89e3\u2026\u2026\uff1f J Gen Virol 80\uff1a1403\u20131421\u3002\u3009View Article\u3000Google Scholar 21.Sawicki SG, Sawicki DL, Siddell SG (2007) A Contemporary View of Coronavirus Transcription. J Virol 81: 20\u201329.\u00a0\u00a0\u00a0 \u300821. Sawicki SG\u3001Sawicki DL\u3001Siddell SG\uff082007\uff09\u51a0\u72c0\u75c5\u6bd2\u8f49\u9304\u7684\u7576\u4ee3\u89c0\u9ede\u3002 J Virol 81\uff1a20-29\u3002\u3009View Article\u3000Google Scholar 22.Butterworth BE, Korant BD (1974) Characterization of the large picornaviral polypeptides produced in the presence of zinc ion. J Virol 14: 282\u2013291.\u00a0\u00a0\u00a0 \u300822. Butterworth BE, Korant BD (1974) \u5728\u92c5\u96e2\u5b50\u5b58\u5728\u4e0b\u7522\u751f\u7684\u5927\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u591a\u80bd\u7684\u8868\u5fb5\u3002 J Virol 14\uff1a282-291\u3002\u3009View Article\u3000Google Scholar 23.Denison MR, Perlman S (1986) Translation and processing of mouse hepatitis virus virion RNA in a cell-free system. J Virol 60: 12\u201318.\u00a0\u00a0\u00a0 \u300823. Denison MR, Perlman S (1986) \u5728\u7121\u7d30\u80de\u7cfb\u7d71\u4e2d\u7ffb\u8b6f\u548c\u8655\u7406\u5c0f\u9f20\u809d\u708e\u75c5\u6bd2\u75c5\u6bd2\u9ad4 RNA\u3002 J Virol 60\uff1a12-18\u3002\u3009View Article\u3000Google Scholar 24.Denison MR, Zoltick PW, Hughes SA, Giangreco B, Olson AL, et al. (1992) Intracellular processing of the N-terminal ORF 1a proteins of the coronavirus MHV-A59 requires multiple proteolytic events. Virology 189: 274\u2013284.\u00a0\u00a0\u00a0 \u300824. Denison MR\u3001Zoltick PW\u3001Hughes SA\u3001Giangreco B\u3001Olson AL \u7b49\u3002 (1992) \u51a0\u72c0\u75c5\u6bd2 MHV-A59 \u7684 N \u7aef ORF 1a \u86cb\u767d\u7684\u7d30\u80de\u5167\u52a0\u5de5\u9700\u8981\u591a\u500b\u86cb\u767d\u6c34\u89e3\u4e8b\u4ef6\u3002\u75c5\u6bd2\u5b78 189\uff1a274-284\u3002\u3009View Article\u3000Google Scholar 25.van Hemert MJ, de Wilde AH, Gorbalenya AE, Snijder EJ (2008) The in Vitro RNA Synthesizing Activity of the Isolated Arterivirus Replication\\\/Transcription Complex Is Dependent on a Host Factor. J Biol Chem 283: 16525\u201316536.\u00a0\u00a0\u00a0 \u300825. van Hemert MJ\u3001de Wilde AH\u3001Gorbalenya AE\u3001Snijder EJ (2008) \u5206\u96e2\u7684\u52d5\u8108\u75c5\u6bd2\u8907\u88fd\\\/\u8f49\u9304\u8907\u5408\u7269\u7684\u9ad4\u5916 RNA \u5408\u6210\u6d3b\u6027\u53d6\u6c7a\u65bc\u5bbf\u4e3b\u56e0\u5b50\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 283\uff1a16525-16536\u3002\u3009View Article\u3000Google Scholar 26.van Hemert MJ, van den Worm SHE, Knoops K, Mommaas AM, Gorbalenya AE, et al. (2008) SARS-Coronavirus Replication\\\/Transcription Complexes Are Membrane-Protected and Need a Host Factor for Activity In Vitro. PLoS Pathog 4: e1000054.\u00a0\u00a0\u00a0 \u300826. van Hemert MJ\u3001van den Worm SHE\u3001Knoops K\u3001Mommaas AM\u3001Gorbalenya AE \u7b49\u3002 (2008) SARS-\u51a0\u72c0\u75c5\u6bd2\u8907\u88fd\\\/\u8f49\u9304\u8907\u5408\u7269\u53d7\u819c\u4fdd\u8b77\uff0c\u9700\u8981\u5bbf\u4e3b\u56e0\u5b50\u624d\u80fd\u9032\u884c\u9ad4\u5916\u6d3b\u52d5\u3002 PLoS \u75c5\u539f\u9ad4 4\uff1ae1000054\u3002\u3009View Article\u3000Google Scholar 27.te Velthuis AJ, Arnold JJ, Cameron CE, van den Worm SH, Snijder EJ (2009) The RNA polymerase activity of SARS-coronavirus nsp12 is primer dependent. Nucleic Acids Res 38: 203\u2013214.\u00a0\u00a0\u00a0 \u300827. te Velthuis AJ\u3001Arnold JJ\u3001Cameron CE\u3001van den Worm SH\u3001Snijder EJ (2009) SARS \u51a0\u72c0\u75c5\u6bd2 nsp12 \u7684 RNA \u805a\u5408\u9176\u6d3b\u6027\u4f9d\u8cf4\u65bc\u5f15\u7269\u3002\u6838\u9178\u7814\u7a76 38\uff1a203-214\u3002\u3009View Article\u3000Google Scholar 28.Beerens N, Selisko B, Ricagno S, Imbert I, van der Zanden L, et al. (2007) De Novo Initiation of RNA Synthesis by the Arterivirus RNA-Dependent RNA Polymerase. J Virol 81: 8384\u20138395.\u00a0\u00a0\u00a0 \u300828. Beerens N\u3001Selisko B\u3001Ricagno S\u3001Imbert I\u3001van der Zanden L \u7b49\u3002 (2007) \u901a\u904e\u52d5\u8108\u75c5\u6bd2 RNA \u4f9d\u8cf4\u7684 RNA \u805a\u5408\u9176\u5f9e\u982d\u958b\u59cb\u5408\u6210 RNA\u3002 J Virol 81\uff1a8384\u20138395\u3002\u3009View Article\u3000Google Scholar 29.van den Born E, Posthuma CC, Knoops K, Snijder EJ (2007) An infectious recombinant equine arteritis virus expressing green fluorescent protein from its replicase gene. J Gen Virol 88: 1196\u20131205.\u00a0\u00a0\u00a0 \u300829. van den Born E, Posthuma CC, Knoops K, Snijder EJ (2007) \u4e00\u7a2e\u50b3\u67d3\u6027\u91cd\u7d44\u99ac\u52d5\u8108\u708e\u75c5\u6bd2\uff0c\u5f9e\u5176\u8907\u5236\u9176\u57fa\u56e0\u8868\u9054\u7da0\u8272\u7192\u5149\u86cb\u767d\u3002 J Gen Virol 88\uff1a1196-1205\u3002\u3009View Article\u3000Google Scholar 30.Sims AC, Burkett SE, Yount B, Pickles RJ (2008) SARS-CoV replication and pathogenesis in an in vitro model of the human conducting airway epithelium. Virus Res 133: 33\u201344.\u00a0\u00a0\u00a0 \u300830. Sims AC\u3001Burkett SE\u3001Yount B\u3001Pickles RJ (2008) SARS-CoV \u5728\u4eba\u985e\u50b3\u5c0e\u6c23\u9053\u4e0a\u76ae\u9ad4\u5916\u6a21\u578b\u4e2d\u7684\u8907\u5236\u548c\u767c\u75c5\u6a5f\u5236\u3002\u75c5\u6bd2\u7814\u7a76 133\uff1a33-44\u3002\u3009View Article\u3000Google Scholar 31.Xu X, Liu Y, Weiss S, Arnold E, Sarafianos SG, et al. (2003) Molecular model of SARS coronavirus polymerase: implications for biochemical functions and drug design. Nucleic Acids Res 31: 7117\u20137130.\u00a0\u00a0\u00a0 \u300831. Xu X\u3001Liu Y\u3001Weiss S\u3001Arnold E\u3001Sarafianos SG \u7b49\u3002 (2003) SARS \u51a0\u72c0\u75c5\u6bd2\u805a\u5408\u9176\u7684\u5206\u5b50\u6a21\u578b\uff1a\u5c0d\u751f\u5316\u529f\u80fd\u548c\u85e5\u7269\u8a2d\u8a08\u7684\u5f71\u97ff\u3002\u6838\u9178\u7814\u7a76 31\uff1a7117-7130\u3002\u3009View Article\u3000Google Scholar 32.Zhai Y, Sun F, Li X, Pang H, Xu X, et al. (2005) Insights into SARS-CoV transcription and replication from the structure of the nsp7-nsp8 hexadecamer. Nat Struct Mol Biol 12: 980\u2013986.\u00a0\u00a0\u00a0 \u300832. \u7fdf\u6bc5\u3001\u5b6b\u98db\u3001\u674e\u65ed\u3001\u9f90\u83ef\u3001\u5f90\u65ed\u7b49\u3002 (2005) \u5f9e nsp7-nsp8 \u5341\u516d\u805a\u9ad4\u7684\u7d50\u69cb\u6d1e\u5bdf SARS-CoV \u8f49\u9304\u548c\u5fa9\u5236\u3002 Nat Struct Mol Biol 12\uff1a980-986\u3002\u3009View Article\u3000Google Scholar 33.Stockman LJ, Bellamy R, Garner P (2006) SARS: Systematic Review of Treatment Effects. PLoS Med 3: e343.\u00a0\u00a0\u00a0 \u300833. Stockman LJ\u3001Bellamy R\u3001Garner P (2006) SARS\uff1a\u6cbb\u7642\u6548\u679c\u7684\u7cfb\u7d71\u8a55\u50f9\u3002 PLoS Med 3: e343\u3002\u3009View Article\u3000Google Scholar 34.Thompson A, Patel K, Tillman H, McHutchison JG (2009) Directly acting antivirals for the treatment of patients with hepatitis C infection: A clinical development update addressing key future challenges. J Hepatol 50: 184\u2013194.\u00a0\u00a0\u00a0 \u300834. Thompson A\u3001Patel K\u3001Tillman H\u3001McHutchison JG (2009) \u7528\u65bc\u6cbb\u7642\u4e19\u578b\u809d\u708e\u611f\u67d3\u60a3\u8005\u7684\u76f4\u63a5\u6297\u75c5\u6bd2\u85e5\u7269\uff1a\u61c9\u5c0d\u672a\u4f86\u95dc\u9375\u6311\u6230\u7684\u81e8\u5e8a\u958b\u767c\u66f4\u65b0\u3002 J Hepatol 50\uff1a184-194\u3002\u3009View Article\u3000Google Scholar 35.De Clercq E (2004) Antivirals and antiviral strategies. Nat Rev Microbiol 2: 704\u2013720.\u00a0\u00a0\u00a0 \u300835. De Clercq E (2004) \u6297\u75c5\u6bd2\u85e5\u7269\u548c\u6297\u75c5\u6bd2\u7b56\u7565\u3002 Nat Rev Microbiol 2: 704\u2013720\u3002\u3009View Article\u3000Google Scholar 36.Thompson AJV, McHutchison JG (2009) Antiviral resistance and specifically targeted therapy for HCV (STAT-C). J Viral Hepat 16: 377\u2013387.\u00a0\u00a0\u00a0 \u300836. Thompson AJV, McHutchison JG (2009) HCV \u6297\u75c5\u6bd2\u8010\u85e5\u6027\u548c\u7279\u7570\u6027\u9776\u5411\u6cbb\u7642 (STAT-C)\u3002 J \u75c5\u6bd2\u6027\u809d\u75c5 16\uff1a377-387\u3002\u3009View Article\u3000Google Scholar 37.Yang W, Lee JY, Nowotny M (2006) Making and Breaking Nucleic Acids: Two-Mg2+-Ion Catalysis and Substrate Specificity. Mol Cell 22: 5\u201313.\u00a0\u00a0\u00a0 \u300837. Yang W\u3001Lee JY\u3001Nowotny M (2006) \u88fd\u9020\u548c\u7834\u58de\u6838\u9178\uff1a\u96d9 Mg2+ \u96e2\u5b50\u50ac\u5316\u548c\u5e95\u7269\u7279\u7570\u6027\u3002\u6469\u723e\u7d30\u80de 22\uff1a5-13\u3002\u3009View Article\u3000Google Scholar 38.Castro C, Smidansky E, Maksimchuk KR, Arnold JJ, Korneeva VS, et al. (2007) Two proton transfers in the transition state for nucleotidyl transfer catalyzed by RNA- and DNA-dependent RNA and DNA polymerases. Proc Natl Acad Sci USA 104: 4267\u20134272.\u00a0\u00a0\u00a0 \u300838. Castro C\u3001Smidansky E\u3001Maksimchuk KR\u3001Arnold JJ\u3001Korneeva VS \u7b49\u3002 (2007) \u7531 RNA \u548c DNA \u4f9d\u8cf4\u6027 RNA \u548c DNA \u805a\u5408\u9176\u50ac\u5316\u7684\u6838\u82f7\u9178\u8f49\u79fb\u7684\u5169\u500b\u904e\u6e21\u614b\u8cea\u5b50\u8f49\u79fb\u3002 Proc Natl Acad Sci USA 104\uff1a4267\u20134272\u3002\u3009View Article\u3000Google Scholar 39.Arnold JJ, Ghosh SK, Cameron CE (1999) Poliovirus RNA-dependent RNA Polymerase (3Dpol). Divalent cation modulation of primer, template and nucleotide selection. J Biol Chem 274: 37060\u201337069.\u00a0\u00a0\u00a0 \u300839. Arnold JJ\u3001Ghosh SK\u3001Cameron CE (1999) \u810a\u9ad3\u7070\u8cea\u708e\u75c5\u6bd2 RNA \u4f9d\u8cf4\u6027 RNA \u805a\u5408\u9176 (3Dpol)\u3002\u5f15\u7269\u3001\u6a21\u677f\u548c\u6838\u82f7\u9178\u9078\u64c7\u7684\u4e8c\u50f9\u967d\u96e2\u5b50\u8abf\u7bc0\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 274\uff1a37060-37069\u3002\u3009View Article\u3000Google Scholar 40.Iuchi S (2001) Three classes of C2H2 zinc finger proteins. Cell Mol Life Sci 58: 625\u2013635.\u00a0\u00a0\u00a0 \u300840. Iuchi S (2001) \u4e09\u985e C2H2 \u92c5\u6307\u86cb\u767d\u3002\u7d30\u80de\u5206\u5b50\u751f\u547d\u79d1\u5b78 58\uff1a625-635\u3002\u3009View Article\u3000Google Scholar 41.Gomis-Ruth XF (2009) Catalytic domain architecture of metzincin metalloproteases. J Biol Chem 284: 15353\u201315357.\u00a0\u00a0\u00a0 \u300841. Gomis-Ruth XF (2009) Metzincin \u91d1\u5c6c\u86cb\u767d\u9176\u7684\u50ac\u5316\u57df\u7d50\u69cb\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 284\uff1a15353\u201315357\u3002\u3009View Article\u3000Google Scholar 42.Yap TL, Xu T, Chen Y-L, Malet H, Egloff M-P, et al. (2007) Crystal Structure of the Dengue Virus RNA-Dependent RNA Polymerase Catalytic Domain at 1.85-Angstrom Resolution. J Virol 81: 4753\u20134765.\u00a0\u00a0\u00a0 \u300842. Yap TL\u3001Xu T\u3001Chen Y-L\u3001Malet H\u3001Egloff M-P \u7b49\u3002 (2007) \u5206\u8fa8\u7387\u70ba 1.85 \u57c3\u7684\u767b\u9769\u71b1\u75c5\u6bd2\u4f9d\u8cf4\u65bc RNA \u7684 RNA \u805a\u5408\u9176\u50ac\u5316\u7d50\u69cb\u57df\u7684\u6676\u9ad4\u7d50\u69cb\u3002 J Virol 81\uff1a4753\u20134765\u3002\u3009View Article\u3000Google Scholar 43.Winek CL, Buehler EV (1966) Intravenous toxicity of zinc pyridinethione and several zinc salts. Toxicol Appl Pharmacol 9: 296\u2013273.\u00a0\u00a0\u00a0 \u300843. Winek CL, Buehler EV (1966) \u5421\u5576\u786b\u916e\u92c5\u548c\u5e7e\u7a2e\u92c5\u9e7d\u7684\u975c\u8108\u6bd2\u6027\u3002 Toxicol Appl Pharmacol 9\uff1a296-273\u3002\u3009View Article\u3000Google Scholar 44.Snyder DR, de Jesus CP, Towfighi J, Jacoby RO, Wedig JH (1979) Neurological, microscopic and enzyme-histochemical assessment of zinc pyrithione toxicity. Food Cosmet Toxicol 17: 651\u2013660.\u00a0\u00a0\u00a0 \u300844. Snyder DR, de Jesus CP, Towfighi J, Jacoby RO, Wedig JH (1979) \u5421\u5576\u786b\u916e\u92c5\u6bd2\u6027\u7684\u795e\u7d93\u5b78\u3001\u986f\u5fae\u93e1\u548c\u9176\u7d44\u7e54\u5316\u5b78\u8a55\u4f30\u3002\u98df\u54c1\u5316\u599d\u54c1\u6bd2\u7406\u5b78 17\uff1a651-660\u3002\u3009View Article\u3000Google Scholar 45.Magda D, Lecane P, Wang Z, Hu W, Thiemann P, et al. (2008) Synthesis and anticancer properties of water-soluble zinc ionophores. Cancer Res 68: 5318\u20135325.\u00a0\u00a0\u00a0 \u300845. Magda D\u3001Lecane P\u3001Wang Z\u3001Hu W\u3001Thiemann P \u7b49\u3002 (2008) \u6c34\u6eb6\u6027\u92c5\u96e2\u5b50\u8f09\u9ad4\u7684\u5408\u6210\u548c\u6297\u764c\u7279\u6027\u3002\u764c\u75c7\u7814\u7a76 68\uff1a5318-5325\u3002\u3009View Article\u3000oogle Scholar 46.Snijder EJ, van der Meer Y, Zevenhoven-Dobbe J, Onderwater JJM, van der Meulen J, et al. (2006) Ultrastructure and Origin of Membrane Vesicles Associated with the Severe Acute Respiratory Syndrome Coronavirus Replication Complex. J Virol 80: 5927\u20135940.\u00a0\u00a0\u00a0 \u300846. Snijder EJ\u3001van der Meer Y\u3001Zevenhoven-Dobbe J\u3001Onderwater JJM\u3001van der Meulen J \u7b49\u3002 (2006) \u8207\u56b4\u91cd\u6025\u6027\u547c\u5438\u7cfb\u7d71\u7d9c\u5408\u75c7\u51a0\u72c0\u75c5\u6bd2\u8907\u88fd\u8907\u5408\u9ad4\u76f8\u95dc\u7684\u819c\u56ca\u6ce1\u7684\u8d85\u5fae\u7d50\u69cb\u548c\u8d77\u6e90\u3002 J Virol 80\uff1a5927-5940\u3002\u3009View Article\u3000Google Scholar 47.Studier F (2005) Protein production by auto-induction in high density shaking cultures. Protein Expr Purif 41: 207\u2013234.\u00a0\u00a0\u00a0 \u300847. Studier F (2005) \u5728\u9ad8\u5bc6\u5ea6\u632f\u76ea\u57f9\u990a\u4e2d\u901a\u904e\u81ea\u52d5\u8a98\u5c0e\u7522\u751f\u86cb\u767d\u8cea\u3002\u86cb\u767d\u8cea Expr Purif 41\uff1a207-234\u3002\u3009View Article\u3000Google Scholar   \u91cd\u5fb7\u4e26\u4e14\u5c07\u5728\u5317\u4eac\u7684\u300c\u4e2d\u570b\u79d1\u5b78\u9662\u751f\u7269\u7269\u7406\u7814\u7a76\u6240\u611f\u67d3\u8207\u514d\u75ab\u5b78\u91cd\u9ede\u5be6\u9a57\u5ba4\u300d\u5728\u91ab\u5b78\u96dc\u8a8c\u4e0a\u6240\u767c\u8868\u7684\u4e00\u7bc7\u82f1\u6587\u7814\u7a76\u5831\u544a\u300a\u92c5\u6307\u6297\u75c5\u6bd2\u86cb\u767d\u6291\u5236\u7532\u578b\u6d41\u611f\u75c5\u6bd2\u86cb\u767d\u8868\u9054\u4ee5\u53ca\u88ab\u75c5\u6bd2\u7de8\u78bcNS1 \u5c0d\u6297\u7684\u7c21\u8981\u5f62\u5f0f\u300b\u7684\u4e2d\u6587\u81ea\u8b6f\u7248\u4e5f\u5bc4\u4e0a\u7d66\u4ed6\u53c3\u8003(\u5982\u9644\u6a94)\u3002 \u4e26\u4e14\uff0c\u91cd\u5fb7\u9084\u767c\u53bb\u4ee5\u4e0b\u5716\u7247\u8207\u8aaa\u660e\uff1a   \u300e\u9019\u5c31\u662f\u92c5\u6307\u7d50\u69cb\u5716\uff0c\u7531\u65bc\u9019\u662f\u87af\u5408\u7d50\u69cb\uff0c\u56e0\u6b64\u5b83\u80fd\u5f88\u5bb9\u6613\u5c07\u92c5\u96e2\u5b50\u7a7f\u900f\u7d30\u80de\u58c1\u800c\u8207\u8eb2\u85cf\u5728\u7d30\u80de\u5167\u840c\u767c\u7684\u75c5\u6bd2\u56e0\u70ba\u767c\u751f\u6291\u5236\u7ffb\u8b6f\u548c\u4fc3\u9032\u75c5\u6bd2 mRNA\u7684\u964d\u89e3\uff0c\u56e0\u6b64\u901a\u904e PARP \u7d50\u69cb\u57df\u800c\u4fc3\u9032 IAV \u86cb\u767d\u964d\u89e3\uff0c\u5be6\u9a57\u5df2\u7d93\u8b49\u5be6\u5b83\u53ef\u8b93\u5165\u4fb5\u5230\u7d30\u80de\u5167\u7684\u75c5\u6bd2\u4e4bRNA\u6a5f\u8f49\u767c\u751f\u4f11\u514b(\u4f46\u4e0d\u4e00\u5b9a\u6703\u6b7b\u4ea1)\uff0c\u800c\u4f7f\u5f97\u539f\u672c\u7121\u751f\u547d\u7684\u75c5\u6bd2\u56e0\u6b64\u4e0d\u80fd\u5927\u91cf\u8907\u88fd\u840c\u751f\uff0c\u800c\u53ef\u4ee5\u70ba\u4eba\u9ad4\u7684\u514d\u75ab\u7cfb\u7d71\u89e3\u6c7a\u3002\u300f \u9019\u4e00\u4f4d\u738b\u59d3\u7db2\u53cb\u53c8\u767c\u4f86\u4ee5\u4e0b\u4fe1\u606f\uff1a\u300e\u80fd\u5920\u6bba\u75c5\u6bd2\u800c\u4e0d\u6703\u6bba\u6b7b\u6211\u5011\u81ea\u5df1\u7684\u7d30\u80de,\u5929\u4e0b\u6709\u9019\u9ebc\u597d\u7684\u4e8b\u55ce\uff1f\u4e0d\u904e\u662f\u71df\u990a\u88dc\u52a9\u54c1\u800c\u5df2\uff01\u300f \u91cd\u5fb7\u770b\u4ed6\u5728\u610f\u6c23\u7528\u4e8b\uff0c\u56e0\u6b64\u5c31\u56de\u8986\u4ed6\u8aaa\uff1a\u300e\u96a8\u60a8\u9ad8\u8208\u600e\u9ebc\u8aaa\u90fd\u597d\uff0c\u6211\u6c92\u6709\u8aaa\u5b83\u662f\u85e5\uff0c\u53ea\u8aaa\u662f\u4fdd\u5065\u54c1\u800c\u5df2\u3002\u300f\u767c\u5b8c\u4e86\u9019\u4fe1\u606f\u4e4b\u5f8c\uff0c\u91cd\u5fb7\u767c\u8a93\u4ee5\u5f8c\u518d\u4e5f\u4e0d\u5c0d\u9019\u7a2e\u96de\u86cb\u88e1\u6311\u9aa8\u982d\u7684\u4eba\u63d0\u4f9b\u4efb\u4f55\u6709\u50f9\u503c\u7684\u8cc7\u8a0a\u4e86\u3002 \u4f46\u662f\uff0c\u91cd\u5fb7\u9084\u662f\u8981\u544a\u8a34\u5927\u5bb6\u70ba\u4f55\u8981\u5efa\u8b70\u5927\u5bb6\u5403\u542b\u92c5\u96e2\u5b50\u7684\u4fdd\u5065\u98df\u54c1\uff0c\u7576\u7136\u92c5\u5f9e\u98df\u7269\u4e2d\u651d\u53d6\u4e5f\u6709\uff0c\u4f46\u662f\u98df\u7269\u5fc5\u9808\u7d93\u904e\u6d88\u5316\u5f8c\u92c5\u96e2\u5b50\u624d\u6703\u900f\u904e\u8840\u6db2\u800c\u5230\u4eba\u9ad4\u5668\u5b98\uff0c\u9019\u500b\u904e\u7a0b\u592a\u9577\u592a\u4e45\u4e86\uff0c\u56e0\u6b64\u92c5\u96e2\u5b50\u53ef\u80fd\u53c8\u8207\u5176\u4ed6\u7269\u8cea\u7d50\u5408\u6210\u5316\u5408\u7269\uff0c\u800c\u518d\u4e0d\u80fd\u767c\u751f\u8207\u8ca0\u96e2\u5b50\u7684\u75c5\u6bd2\u4e2d\u548c\u7684\u6548\u679c\u3002\u56e0\u6b64\u5c31\u66f4\u6c92\u6709\u767c\u63ee\u5230\u92c5\u96e2\u5b50\u6240\u7368\u5177\u7684\u87af\u5408\u4ea4\u63db\u6548\u679c\u3002 \u7531\u65bc\uff0c\u611f\u5192\u6216\u662fCOVID-19\u7684\u521d\u671f\u75c5\u72c0\u4e3b\u8981\u90fd\u662f\u91dd\u5c0d\u4e0a\u652f\u6c23\u7ba1\uff0c\u56e0\u6b64\u4ee3\u8868\u547c\u5438\u7ba1\u9053\u7684\u75c5\u6bd2\u91cf\u6700\u5927\uff0c\u56e0\u6b64\u53ef\u4ee5\u542b\u5728\u5634\u4e2d\u6162\u6162\u5206\u89e3\u7684\u5589\u7cd6\u4e4b\u6548\u679c\u6700\u597d\uff0c\u56e0\u70ba\u5b83\u8b93\u901a\u904e\u7684\u6c23\u6d41\u4e2d\u7684\u75c5\u6bd2\u5148\u88ab\u92c5\u96e2\u5b50\u4e2d\u548c\uff0c\u800c\u7cd6\u6c41\u6d41\u5165\u98df\u9053\u6642\u92c5\u96e2\u5b50\u4e5f\u6703\u6ea2\u5165\u4e0a\u652f\u6c23\u7ba1\u3002\u9019\u5c31\u662f\u70ba\u4f55\u91cd\u5fb7\u9996\u5148\u63a8\u85a6\u5df2\u7d93\u5728\u7f8e\u570b\u98a8\u884c30\u591a\u5e74\u7684\u611f\u5192\u9810\u9632\u7528\u85e5Cold Eeze\u4e2d\u6587\u8b6f\u540d\u70ba\u3010\u92c5\u7cd6\u3011\uff0c\u56e0\u70ba\u5b83\u5df2\u7d93\u904e\u4e86\u5c08\u5229\u4fdd\u8b77\u671f\uff0c\u5b78\u540d\u85e5\u540d\u662fGeneric Version\uff0c\u56e0\u70ba\u552e\u50f9\u8f03\u4fbf\u5b9c(\u4f46\u662f\u73fe\u5728\u5168\u7403\u6436\u8cfc\u5f88\u53ef\u80fd\u7f3a\u8ca8)\u3002 \u73fe\u5728\uff0c\u91cd\u5fb7\u8981\u544a\u8a34\u5927\u5bb6\u4e00\u500b\u79d8\u5bc6\uff0c\u90a3\u5c31\u662f\u70ba\u4f55\u91cd\u5fb7\u8981\u5c0d\u92c5\u96e2\u5b50\u9032\u884c\u7814\u7a76\u5462\uff0c\u6545\u4e8b\u662f\u9019\u6a23\u5b50\u7684\uff0c\u7531\u65bc\u4e00\u5e74\u524d \u91cd\u5fb7 \u8acb\u793a\u201c\u9748\u7236\u201d(\u5143\u59cb\u5929\u5c0a)\u8aaa\uff1a\u300e\u65e2\u7136\u00a0 \u9748\u7236\u80fd\u63d0\u524d\u534a\u5e74\u8b66\u544a\u6211\u5011\u5c07\u6709\u6b64\u5927\u75ab\u60c5\uff0c\u662f\u5426\u80fd\u544a\u77e5\u5f92\u5152\u5982\u4f55\u88fd\u5f97\u89e3\u85e5\uff1f\u300f\u64f2\u832d\u61c9\u8a31\u5f8c\uff0c\u91cd\u5fb7 \u6b63\u4e0d\u77e5\u5982\u4f55\u518d\u554f\u9019\u89e3\u85e5\u79d8\u65b9\u6642\uff0c\u8166\u4e2d\u7a81\u7136\u6e67\u73fe\u4e00\u500b\u201c\u92c5\u201d\u5b57\uff0c\u64f2\u832d\u518d\u554f\u78ba\u5b9a\u795e\u8aed\u5c31\u662f\u6b64\u201c\u92c5\u201d\u5b57\u7121\u8aa4\u3002 \u5728\u6b64\u4e4b\u5f8c\uff0c\u91cd\u5fb7\u624d\u958b\u59cb\u8a73\u95b1\u5404\u7a2e\u82f1\u6587\u91ab\u5b78\u6587\u737b\uff0c\u8d8a\u767c\u8b49\u5be6\u92c5\u96e2\u5b50\u6709\u6291\u5236mRNA\u75c5\u6bd2\u7684\u6548\u679c\uff0c\u800c\u4e14\u56e0\u70ba\u92c5\u96e2\u5b50\u662f\u63a1\u53d6\u87af\u5408\u65b9\u5f0f\u8b93\u92c5\u96e2\u5b50\u7a7f\u900f\u7d30\u80de\u58c1\u9032\u5165\u4f86\u964d\u89e3\u75c5\u6bd2\u86cb\u767d\u8907\u88fd\u6240\u9700\u6c42\u7684PARP \u7d50\u69cb\u57df\uff0c\u56e0\u6b64\u9019\u662f\u4e00\u7a2e\u7cbe\u6e96\u7834\u7a97\u5f0f\u7684\u65ac\u9996\u884c\u52d5\uff0c\u5c0d\u65bc\u7d30\u80de\u7684\u50b7\u5bb3\u751a\u5c0f\uff0c\u56e0\u6b64\u5c0d\u65bc\u4eba\u9ad4\u7684\u5371\u5bb3\u751a\u4f4e\u3002 \u91cd\u5fb7\u4e00\u5e74\u524d\u5c31\u628a\u7814\u7a76\u6210\u679c\u516c\u958b\uff0c\u4e00\u4f86\u56e0\u70ba\u81ea\u5df1\u962e\u56ca\u7f9e\u6f80\uff0c\u7121\u6cd5\u7e7c\u7e8c\u7814\u767c\uff0c\u4e8c\u4f86\u91cd\u5fb7\u5df2\u7d93\u9810\u7aba\u5230\u672a\u4f86\u9084\u6709\u5176\u4ed6\u56b4\u91cd\u75be\u75c5\uff0c\u800c\u4e14COVID-19\u9084\u6703\u5927\u7a81\u8b8a\uff0c\u56e0\u6b64\u8b93\u5176\u4ed6\u4eba\u63a5\u68d2\u4f86\u7814\u7a76\u6216\u8a31\u6703\u66f4\u6709\u6210\u6548\u3002\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/?p=104514#breadcrumb\"},\"inLanguage\":\"zh-TW\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/pingtungtimes.com.tw\\\/?p=104514\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"zh-TW\",\"@id\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/?p=104514#primaryimage\",\"url\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/wp-content\\\/uploads\\\/2022\\\/05\\\/\u4e00\u822c\u4eba\u5f9e\u98df\u7269\u4e2d\u651d\u53d6\u92c5\u70ba\u7684\u5c31\u662f\u58ef\u967d\u8207\u589e\u9aee.jpg\",\"contentUrl\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/wp-content\\\/uploads\\\/2022\\\/05\\\/\u4e00\u822c\u4eba\u5f9e\u98df\u7269\u4e2d\u651d\u53d6\u92c5\u70ba\u7684\u5c31\u662f\u58ef\u967d\u8207\u589e\u9aee.jpg\",\"width\":897,\"height\":468,\"caption\":\"\u25bc\u4e00\u822c\u4eba\u5f9e\u98df\u7269\u4e2d\u651d\u53d6\u201c\u92c5\u201d\u70ba\u7684\u5c31\u662f\u58ef\u967d\u8207\u589e\u9aee\u3002\uff08\u5716\uff0f\u674e\u91cd\u5fb7\u63d0\u4f9b\uff09\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/?p=104514#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"\u9996\u9801\",\"item\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"\u597d\u201c\u92c5\u201d\u3000\u4e5f\u6703\u60f9\u4e86\u4e00\u8eab\u8165\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/#website\",\"url\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/\",\"name\":\"\u5c4f\u6771\u6642\u5831\u96fb\u5b50\u5831\",\"description\":\"\u5c4f\u6771\u6642\u5831\u96fb\u5b50\u5831PingtungTimes\uff0c\u6700\u95dc\u5fc3\u5728\u5730\u7684\u5a92\u9ad4\",\"publisher\":{\"@id\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"zh-TW\"},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/#organization\",\"name\":\"\u5c4f\u6771\u6642\u5831\u96fb\u5b50\u5831\",\"url\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"zh-TW\",\"@id\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/#\\\/schema\\\/logo\\\/image\\\/\",\"url\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/wp-content\\\/uploads\\\/2023\\\/02\\\/logo-150.png\",\"contentUrl\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/wp-content\\\/uploads\\\/2023\\\/02\\\/logo-150.png\",\"width\":150,\"height\":150,\"caption\":\"\u5c4f\u6771\u6642\u5831\u96fb\u5b50\u5831\"},\"image\":{\"@id\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/#\\\/schema\\\/logo\\\/image\\\/\"},\"sameAs\":[\"https:\\\/\\\/www.facebook.com\\\/pingtungnews\"]},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/#\\\/schema\\\/person\\\/90cf880d4d210d8db447ae0199435aa4\",\"name\":\"fanjiang\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"zh-TW\",\"@id\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/392bb75508611fa0e892a64a45ae50b3be151c3f249f4fc2e86107740441ff2b?s=96&d=blank&r=g\",\"url\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/392bb75508611fa0e892a64a45ae50b3be151c3f249f4fc2e86107740441ff2b?s=96&d=blank&r=g\",\"contentUrl\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/392bb75508611fa0e892a64a45ae50b3be151c3f249f4fc2e86107740441ff2b?s=96&d=blank&r=g\",\"caption\":\"fanjiang\"},\"url\":\"https:\\\/\\\/pingtungtimes.com.tw\\\/?author=1\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"\u597d\u201c\u92c5\u201d\u3000\u4e5f\u6703\u60f9\u4e86\u4e00\u8eab\u8165 - \u5c4f\u6771\u6642\u5831\u96fb\u5b50\u5831","description":"\u3010\u674e\u91cd\u5fb7\uff0f\u5c08\u6b04\u5831\u5c0e2022\/05\/27\u3011\u3000\u4eca\u65e9\uff0c\u6709\u4e00\u78ba\u8a3a\u7652\u5f8c\u8005\u8207\u91cd\u5fb7\u8a0e\u8ad6\u4ed6\u7684\u75c5\u60c5\uff0c\u91cd\u5fb7\u5efa\u8b70\u4ed6\u88dc\u5145\u92c5\u96e2\u5b50\u98df\u54c1\uff0c\u4e26\u5c07\u6b64\u4e00\u4fe1\u606f\u4e5f\u767c\u7d66\u5927\u5bb6\u77e5\u66c9\uff1a \u300e\u6709\u78ba\u8a3a\u7652\u5f8c\u8005\u554f\u91cd\u5fb7\u8981\u5982\u4f55\u4fdd\u5065\u4ee5\u9632\u5fa9\u967d\uff0c\u91cd\u5fb7 \u7686\u5efa\u8b70\u4ed6\u5403\u3010\u92c5\u7cd6\u3011(\u985e\u4f3c\u5589\u7247)\u5c0f\u5b69\u5247\u5403\u3010\u5152\u7ae5\u92c5\u8edf\u7cd6\u3011\uff0c\u6216\u662f\u3010\u9175\u6bcd\u92c5\u3011\u3001\u3010\u87af\u5408\u92c5\u3011\u6216\u662f\u3010\u8461\u8404\u7cd6\u9178\u92c5\u3011\u4f86\u6d88\u6ec5\u6f5b\u85cf\u4eba\u9ad4\u5c1a\u672a\u767c\u4f5c\u7684\u75c5\u6bd2\u3002\u300f \u5f8c\u4f86\u5c31\u63a5\u7372\u4e00\u4f4d\u738b\u59d3\u7db2\u53cb\u56de\u8986\u8aaa\uff1a\u300e\u4e0d\u5c0d\u5594\uff01\u92c5,\u4eba\u985e\u5b58\u6d3b\u6240\u9700\u8981\u7684\u5143\u7d20\u4e4b\u4e00,\u5f9e\u5929\u7136\u98df\u7269\u76f4\u63a5\u651d\u53d6\u5c31\u8db3\u5920\u4e86,\u4eba\u70ba\u8d85\u91cf\u7684\u88dc\u5145\u6703\u51fa\u554f\u984c\u7684\uff01\u92c5,\u4e0d\u6703\u76f4\u63a5\u6bba\u75c5\u6bd2\u7684\uff01\u300f \u9019\u4e00\u4f4d\u738b\u5148\u751f\uff0c\u5927\u6982\u6f0f\u770b\u4e86\u91cd\u5fb7\u5728\u4e00\u5e74\u524d\u5728\u672c\u6703\u7fa4\u7d44\u4e0a\u767c\u8868\u7684\u5e7e\u7bc7\u4ee5\u92c5\u96e2\u5b50\u4f86\u6d88\u6ec5\u75c5\u6bd2\u7684\u6587\u7ae0\uff0c\u56e0\u6b64\uff0c\u91cd\u5fb7\u5c31\u56de\u8986\u4ed6\u8aaa\uff1a\u300e\u6211\u770b\u904e\u4e86\u8a31\u591a\u82f1\u6587\u91ab\u5b78\u6587\u737b\uff0c\u4e5f\u8207\u9ec3\u25cb\u5bcc\u535a\u58eb\u548c\u9673\u25cb\u5e73\u91ab\u5e2b\u8a0e\u8ad6\u904e\u3002\u60a8\u5982\u679c\u60f3\u8981\u770b\u9019\u4e00\u4e9b\u82f1\u6587\u91ab\u5b78\u6587\u737b\uff0c\u6211\u53ef\u4ee5\u5bc4\u53bb\u7d66\u60a8\u3002\u300f\u7136\u5f8c \u91cd\u5fb7 \u5c31\u5c31\u5bc4\u53bb\u7d66\u4ed6\u4ee5\u4e0b\u7684\u8cc7\u6599\uff1a References\u53c3\u8003\u6587\u737b 1.Lazarczyk M, Favre M (2008) Role of Zn2+ ions in host-virus interactions. J Virol 82: 11486\u201311494.\u00a0\u00a0\u00a0 \u30081. Lazarczyk M, Favre M (2008) Zn2+ \u96e2\u5b50\u5728\u5bbf\u4e3b-\u75c5\u6bd2\u76f8\u4e92\u4f5c\u7528\u4e2d\u7684\u4f5c\u7528\u3002 J Virol 82\uff1a11486\u201311494\u3002\u3009\u3000View Article\u3000Google Scholar 2.Frederickson CJ, Koh JY, Bush AI (2005) Neurobiology of zinc in health and disease. Nat Rev Neurosci 6: 449\u2013462.\u00a0\u00a0\u00a0 \u30082. Frederickson CJ\u3001Koh JY\u3001Bush AI (2005) \u92c5\u5728\u5065\u5eb7\u548c\u75be\u75c5\u4e2d\u7684\u795e\u7d93\u751f\u7269\u5b78\u3002 Nat Rev Neurosci 6\uff1a449-462\u3002\u3009View Article\u3000Google Scholar 3.Alirezaei M, Nairn AC, Glowinski J, Premont J, Marin P (1999) Zinc inhibits protein synthesis in neurons: potential rol of phosphorylation of translation initiation factor-2a. J Biol Chem 274: 32433\u201332438.\u00a0\u00a0\u00a0 \u30083. Alirezaei M\u3001Nairn AC\u3001Glowinski J\u3001Premont J\u3001Marin P (1999) \u92c5\u6291\u5236\u795e\u7d93\u5143\u4e2d\u7684\u86cb\u767d\u8cea\u5408\u6210\uff1a\u7ffb\u8b6f\u8d77\u59cb\u56e0\u5b50 2a \u78f7\u9178\u5316\u7684\u6f5b\u5728\u4f5c\u7528\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 274\uff1a32433\u201332438\u3002\u3009View Article\u3000Google Scholar 4.Uchide N, Ohyama K, Bessho T, Yuan B, Yamakawa T (2002) Effect of antioxidants on apoptosis induced by influenza virus infection: inhibition of viral gene replication and transcription with pyrrolidine dithiocarbamate. Antiviral Res 56: 207\u2013217.\u00a0\u00a0\u00a0 \u30084. Uchide N, Ohyama K, Bessho T, Yuan B, Yamakawa T (2002) \u6297\u6c27\u5316\u5291\u5c0d\u6d41\u611f\u75c5\u6bd2\u611f\u67d3\u8a98\u5c0e\u7684\u7d30\u80de\u51cb\u4ea1\u7684\u5f71\u97ff\uff1a\u7528\u5421\u54af\u70f7\u4e8c\u786b\u4ee3\u6c28\u57fa\u7532\u9178\u916f\u6291\u5236\u75c5\u6bd2\u57fa\u56e0\u8907\u5236\u548c\u8f49\u9304\u3002\u6297\u75c5\u6bd2\u7814\u7a76 56\uff1a207-217\u3002\u3009View Article\u3000Google Scholar 5.Suara RO, Crowe JEJ (2004) Effect of zinc salts on respiratory syncytial virus replication. Antimicrob Agents Chemother 48: 783\u2013790.\u00a0\u00a0\u00a0 \u30085. Suara RO, Crowe JEJ (2004) \u92c5\u9e7d\u5c0d\u547c\u5438\u9053\u5408\u80de\u75c5\u6bd2\u8907\u88fd\u7684\u5f71\u97ff\u3002\u6297\u5fae\u751f\u7269\u5291 Chemother 48\uff1a783-790\u3002\u3009View Article\u3000Google Scholar 6.Gaudernak E, Seipelt J, Triendl A, Grassauer A, Kuechler E (2002) Antiviral Effects of Pyrrolidine Dithiocarbamate on Human Rhinoviruses. J Virol 76: 6004\u20136015.\u00a0\u00a0\u00a0 \u30086. Gaudernak E\u3001Seipelt J\u3001Triendl A\u3001Grassauer A\u3001Kuechler E (2002) \u5421\u54af\u70f7\u4e8c\u786b\u4ee3\u6c28\u57fa\u7532\u9178\u916f\u5c0d\u4eba\u9f3b\u75c5\u6bd2\u7684\u6297\u75c5\u6bd2\u4f5c\u7528\u3002 J Virol 76\uff1a6004-6015\u3002\u3009View Article\u3000Google Scholar 7.Si X, McManus BM, Zhang J, Yuan J, Cheung C, et al. (2005) Pyrrolidine Dithiocarbamate Reduces Coxsackievirus B3 Replication through Inhibition of the Ubiquitin-Proteasome Pathway. J Virol 79: 8014\u20138023.\u00a0\u00a0\u00a0 \u30087. Si X, McManus BM, Zhang J, Yuan J, Cheung C, et al. (2005) Pyrrolidine Dithiocarbamate \u901a\u904e\u6291\u5236\u6cdb\u7d20-\u86cb\u767d\u9176\u9ad4\u9014\u5f91\u6e1b\u5c11\u67ef\u85a9\u5947\u75c5\u6bd2 B3 \u8907\u88fd\u3002 J Virol 79\uff1a8014-8023\u3002\u3009View Article\u3000Google Scholar 8.Korant BD, Kauer JC, Butterworth BE (1974) Zinc ions inhibit replication of rhinoviruses. Nature 248: 588\u2013590.\u00a0\u00a0\u00a0 \u30088. Korant BD, Kauer JC, Butterworth BE (1974) \u92c5\u96e2\u5b50\u6291\u5236\u9f3b\u75c5\u6bd2\u7684\u8907\u5236\u3002\u81ea\u7136 248\uff1a588-590\u3002\u3009View Article\u3000Google Scholar 9.Polatnick J, Bachrach HL (1978) Effect of zinc and other chemical agents on foot-and-mouth-disease virus replication. Antimicrob Agents Chemother 13: 731\u2013734.\u00a0\u00a0 \u00a0\u30089. Polatnick J, Bachrach HL (1978) \u92c5\u548c\u5176\u4ed6\u5316\u5b78\u8a66\u5291\u5c0d\u53e3\u8e44\u75ab\u75c5\u6bd2\u8907\u88fd\u7684\u5f71\u97ff\u3002\u6297\u5fae\u751f\u7269\u5291 Chemother 13\uff1a731-734\u3002\u3009View Article\u3000Google Scholar 10.Lanke K, Krenn BM, Melchers WJG, Seipelt J, van Kuppeveld FJM (2007) PDTC inhibits picornavirus polyprotein processing and RNA replication by transporting zinc ions into cells. J Gen Virol 88: 1206\u20131217.\u00a0\u00a0\u00a0 \u300810. Lanke K\u3001Krenn BM\u3001Melchers WJG\u3001Seipelt J\u3001van Kuppeveld FJM (2007) PDTC \u901a\u904e\u5c07\u92c5\u96e2\u5b50\u8f49\u904b\u5230\u7d30\u80de\u4e2d\u4f86\u6291\u5236\u5c0f\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u591a\u86cb\u767d\u52a0\u5de5\u548c RNA \u8907\u88fd\u3002 J Gen Virol 88\uff1a1206-1217\u3002\u3009View Article\u3000Google Scholar7 11.Krenn BM, Gaudernak E, Holzer B, Lanke K, Van Kuppeveld FJM, et al. (2009) Antiviral Activity of the Zinc Ionophores Pyrithione and Hinokitiol against Picornavirus Infections. J Virol 83: 58\u201364.\u00a0\u00a0\u00a0 \u300811. Krenn BM\u3001Gaudernak E\u3001Holzer B\u3001Lanke K\u3001Van Kuppeveld FJM \u7b49\u3002 (2009) \u92c5\u96e2\u5b50\u8f09\u9ad4\u5421\u5576\u786b\u916e\u548c\u6a9c\u9187\u5c0d\u5c0f\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u611f\u67d3\u7684\u6297\u75c5\u6bd2\u6d3b\u6027\u3002 J Virol 83\uff1a58-64\u3002\u3009View Article\u3000Google Scholar 12.Zalewski PD, Forbes IJ, Betts WH (1993) Correlation of apoptosis with change in intracellular labile Zn(II) using Zinquin , a new specific fluorescent probe for Zn(II). Biochem J 296: 403\u2013408.\u00a0 \u300812. Zalewski PD, Forbes IJ, Betts WH (1993) \u4f7f\u7528 Zinquin \uff0c\u7d30\u80de\u51cb\u4ea1\u8207\u7d30\u80de\u5167\u4e0d\u7a69\u5b9a\u92c5\uff08II\uff09\u8b8a\u5316\u7684\u76f8\u95dc\u6027\uff0c\u4e00\u7a2e\u65b0\u7684\u7279\u7570\u6027Zn(II) \u7684\u7192\u5149\u63a2\u91dd\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 296\uff1a403-408\u3002\u3009View Article\u3000Google Scholar 13.Baum EZ, Bebernitz GA, Palant O, Mueller T, Plotch SJ (1991) Purification, properties, and mutagenesis of poliovirus 3C protease. Virology 165: 140\u2013150.\u00a0\u00a0\u00a0 \u300813. Baum EZ\u3001Bebernitz GA\u3001Palant O\u3001Mueller T\u3001Plotch SJ (1991) \u810a\u9ad3\u7070\u8cea\u708e\u75c5\u6bd2 3C \u86cb\u767d\u9176\u7684\u7d14\u5316\u3001\u7279\u6027\u548c\u8a98\u8b8a\u3002\u75c5\u6bd2\u5b78 165\uff1a140-150\u3002\u3009View Article\u3000Google Scholar 14.Cordingley MG, Register RB, Callahan PL, Garsky VM, Colonno RJ (1989) Cleavage of small peptides in vitro by human rhinovirus 14 3C protease expressed in Escherichia coli. J Virol 63: 5037\u20135045.\u00a0\u00a0\u00a0 \u300814. Cordingley MG\u3001Register RB\u3001Callahan PL\u3001Garsky VM\u3001Colonno RJ (1989) \u5728\u5927\u8178\u687f\u83cc\u4e2d\u8868\u9054\u7684\u4eba\u9f3b\u75c5\u6bd2 14 3C \u86cb\u767d\u9176\u9ad4\u5916\u88c2\u89e3\u5c0f\u80bd\u3002 J Virol 63\uff1a5037-5045\u3002\u3009View Article\u3000Google Scholar 15.Ferrari E, Wright-Minogue J, Fang JW, Baroudy BM, Lau JY, et al. (1999) Characterization of soluble hepatitis C virus RNA-dependent RNA polymerase expressed in Escherichia coli. J Virol 73: 1649\u20131654.\u00a0\u00a0\u00a0 \u300815. Ferrari E\u3001Wright-Minogue J\u3001Fang JW\u3001Baroudy BM\u3001Lau JY \u7b49\u3002 (1999) \u5728\u5927\u8178\u687f\u83cc\u4e2d\u8868\u9054\u7684\u53ef\u6eb6\u6027\u4e19\u578b\u809d\u708e\u75c5\u6bd2 RNA \u4f9d\u8cf4\u6027 RNA \u805a\u5408\u9176\u7684\u8868\u5fb5\u3002 J Virol 73\uff1a1649-1654\u3002\u3009View Article\u3000Google Scholar 16.Hung M, Gibbs CS, Tsiang M (2002) Biochemical characterization of rhinovirus RNA-dependent RNA polymerase. Antiviral Res 56: 99\u2013114.\u00a0\u00a0\u00a0 \u300816. Hung M\u3001Gibbs CS\u3001Tsiang M (2002) \u9f3b\u75c5\u6bd2 RNA \u4f9d\u8cf4\u6027 RNA \u805a\u5408\u9176\u7684\u751f\u5316\u7279\u5fb5\u3002\u6297\u75c5\u6bd2\u7814\u7a76 56\uff1a99-114\u3002\u3009View Article\u3000Google Scholar 17.Perlman S, Netland J (2009) Coronaviruses post-SARS: update on replication and pathogenesis. Nat Rev Micro 7: 439\u2013450.\u00a0\u00a0\u00a0 \u300817. Perlman S, Netland J (2009) SARS \u5f8c\u7684\u51a0\u72c0\u75c5\u6bd2\uff1a\u8907\u88fd\u548c\u767c\u75c5\u6a5f\u5236\u7684\u66f4\u65b0\u3002 Nat Rev Micro 7\uff1a439-450\u3002\u3009View Article\u3000Google Scholar 18.Gorbalenya AE, Enjuanes L, Ziebuhr J, Snijder EJ (2006) Nidovirales: evolving the largest RNA virus genome. Virus Res 117: 17\u201337.\u00a0\u00a0\u00a0 \u300818. Gorbalenya AE\u3001Enjuanes L\u3001Ziebuhr J\u3001Snijder EJ (2006) Nidovirales\uff1a\u9032\u5316\u6700\u5927\u7684 RNA \u75c5\u6bd2\u57fa\u56e0\u7d44\u3002\u75c5\u6bd2\u7814\u7a76 117\uff1a17-37\u3002\u3009View Article\u3000Google Scholar 19.Snijder EJ, Bredenbeek PJ, Dobbe JC, Thiel V, Ziebuhr J, et al. (2003) Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage. J Mol Biol 331: 991\u20131004.\u00a0\u00a0\u00a0 \u300819. Snijder EJ\u3001Bredenbeek PJ\u3001Dobbe JC\u3001Thiel V\u3001Ziebuhr J \u7b49\u3002 (2003) SARS \u51a0\u72c0\u75c5\u6bd2\u57fa\u56e0\u7d44\u548c\u86cb\u767d\u8cea\u7d44\u7684\u7368\u7279\u548c\u4fdd\u5b88\u7279\u5fb5\uff0c\u5f9e\u51a0\u72c0\u75c5\u6bd2\u7d44 2 \u8b5c\u7cfb\u7684\u65e9\u671f\u5206\u88c2\u3002 J Mol Biol 331\uff1a991-1004\u3002\u3009View Article\u3000Google Scholar 20.Pasternak AO, Spaan WJ, Snijder EJ (2006) Nidovirus transcription: how to make sense\u2026? J Gen Virol 80: 1403\u20131421.\u00a0\u00a0\u00a0 \u300820. Pasternak AO\u3001Spaan WJ\u3001Snijder EJ (2006) Nidovirus \u8f49\u9304\uff1a\u5982\u4f55\u7406\u89e3\u2026\u2026\uff1f J Gen Virol 80\uff1a1403\u20131421\u3002\u3009View Article\u3000Google Scholar 21.Sawicki SG, Sawicki DL, Siddell SG (2007) A Contemporary View of Coronavirus Transcription. J Virol 81: 20\u201329.\u00a0\u00a0\u00a0 \u300821. Sawicki SG\u3001Sawicki DL\u3001Siddell SG\uff082007\uff09\u51a0\u72c0\u75c5\u6bd2\u8f49\u9304\u7684\u7576\u4ee3\u89c0\u9ede\u3002 J Virol 81\uff1a20-29\u3002\u3009View Article\u3000Google Scholar 22.Butterworth BE, Korant BD (1974) Characterization of the large picornaviral polypeptides produced in the presence of zinc ion. J Virol 14: 282\u2013291.\u00a0\u00a0\u00a0 \u300822. Butterworth BE, Korant BD (1974) \u5728\u92c5\u96e2\u5b50\u5b58\u5728\u4e0b\u7522\u751f\u7684\u5927\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u591a\u80bd\u7684\u8868\u5fb5\u3002 J Virol 14\uff1a282-291\u3002\u3009View Article\u3000Google Scholar 23.Denison MR, Perlman S (1986) Translation and processing of mouse hepatitis virus virion RNA in a cell-free system. J Virol 60: 12\u201318.\u00a0\u00a0\u00a0 \u300823. Denison MR, Perlman S (1986) \u5728\u7121\u7d30\u80de\u7cfb\u7d71\u4e2d\u7ffb\u8b6f\u548c\u8655\u7406\u5c0f\u9f20\u809d\u708e\u75c5\u6bd2\u75c5\u6bd2\u9ad4 RNA\u3002 J Virol 60\uff1a12-18\u3002\u3009View Article\u3000Google Scholar 24.Denison MR, Zoltick PW, Hughes SA, Giangreco B, Olson AL, et al. (1992) Intracellular processing of the N-terminal ORF 1a proteins of the coronavirus MHV-A59 requires multiple proteolytic events. Virology 189: 274\u2013284.\u00a0\u00a0\u00a0 \u300824. Denison MR\u3001Zoltick PW\u3001Hughes SA\u3001Giangreco B\u3001Olson AL \u7b49\u3002 (1992) \u51a0\u72c0\u75c5\u6bd2 MHV-A59 \u7684 N \u7aef ORF 1a \u86cb\u767d\u7684\u7d30\u80de\u5167\u52a0\u5de5\u9700\u8981\u591a\u500b\u86cb\u767d\u6c34\u89e3\u4e8b\u4ef6\u3002\u75c5\u6bd2\u5b78 189\uff1a274-284\u3002\u3009View Article\u3000Google Scholar 25.van Hemert MJ, de Wilde AH, Gorbalenya AE, Snijder EJ (2008) The in Vitro RNA Synthesizing Activity of the Isolated Arterivirus Replication\/Transcription Complex Is Dependent on a Host Factor. J Biol Chem 283: 16525\u201316536.\u00a0\u00a0\u00a0 \u300825. van Hemert MJ\u3001de Wilde AH\u3001Gorbalenya AE\u3001Snijder EJ (2008) \u5206\u96e2\u7684\u52d5\u8108\u75c5\u6bd2\u8907\u88fd\/\u8f49\u9304\u8907\u5408\u7269\u7684\u9ad4\u5916 RNA \u5408\u6210\u6d3b\u6027\u53d6\u6c7a\u65bc\u5bbf\u4e3b\u56e0\u5b50\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 283\uff1a16525-16536\u3002\u3009View Article\u3000Google Scholar 26.van Hemert MJ, van den Worm SHE, Knoops K, Mommaas AM, Gorbalenya AE, et al. (2008) SARS-Coronavirus Replication\/Transcription Complexes Are Membrane-Protected and Need a Host Factor for Activity In Vitro. PLoS Pathog 4: e1000054.\u00a0\u00a0\u00a0 \u300826. van Hemert MJ\u3001van den Worm SHE\u3001Knoops K\u3001Mommaas AM\u3001Gorbalenya AE \u7b49\u3002 (2008) SARS-\u51a0\u72c0\u75c5\u6bd2\u8907\u88fd\/\u8f49\u9304\u8907\u5408\u7269\u53d7\u819c\u4fdd\u8b77\uff0c\u9700\u8981\u5bbf\u4e3b\u56e0\u5b50\u624d\u80fd\u9032\u884c\u9ad4\u5916\u6d3b\u52d5\u3002 PLoS \u75c5\u539f\u9ad4 4\uff1ae1000054\u3002\u3009View Article\u3000Google Scholar 27.te Velthuis AJ, Arnold JJ, Cameron CE, van den Worm SH, Snijder EJ (2009) The RNA polymerase activity of SARS-coronavirus nsp12 is primer dependent. Nucleic Acids Res 38: 203\u2013214.\u00a0\u00a0\u00a0 \u300827. te Velthuis AJ\u3001Arnold JJ\u3001Cameron CE\u3001van den Worm SH\u3001Snijder EJ (2009) SARS \u51a0\u72c0\u75c5\u6bd2 nsp12 \u7684 RNA \u805a\u5408\u9176\u6d3b\u6027\u4f9d\u8cf4\u65bc\u5f15\u7269\u3002\u6838\u9178\u7814\u7a76 38\uff1a203-214\u3002\u3009View Article\u3000Google Scholar 28.Beerens N, Selisko B, Ricagno S, Imbert I, van der Zanden L, et al. (2007) De Novo Initiation of RNA Synthesis by the Arterivirus RNA-Dependent RNA Polymerase. J Virol 81: 8384\u20138395.\u00a0\u00a0\u00a0 \u300828. Beerens N\u3001Selisko B\u3001Ricagno S\u3001Imbert I\u3001van der Zanden L \u7b49\u3002 (2007) \u901a\u904e\u52d5\u8108\u75c5\u6bd2 RNA \u4f9d\u8cf4\u7684 RNA \u805a\u5408\u9176\u5f9e\u982d\u958b\u59cb\u5408\u6210 RNA\u3002 J Virol 81\uff1a8384\u20138395\u3002\u3009View Article\u3000Google Scholar 29.van den Born E, Posthuma CC, Knoops K, Snijder EJ (2007) An infectious recombinant equine arteritis virus expressing green fluorescent protein from its replicase gene. J Gen Virol 88: 1196\u20131205.\u00a0\u00a0\u00a0 \u300829. van den Born E, Posthuma CC, Knoops K, Snijder EJ (2007) \u4e00\u7a2e\u50b3\u67d3\u6027\u91cd\u7d44\u99ac\u52d5\u8108\u708e\u75c5\u6bd2\uff0c\u5f9e\u5176\u8907\u5236\u9176\u57fa\u56e0\u8868\u9054\u7da0\u8272\u7192\u5149\u86cb\u767d\u3002 J Gen Virol 88\uff1a1196-1205\u3002\u3009View Article\u3000Google Scholar 30.Sims AC, Burkett SE, Yount B, Pickles RJ (2008) SARS-CoV replication and pathogenesis in an in vitro model of the human conducting airway epithelium. Virus Res 133: 33\u201344.\u00a0\u00a0\u00a0 \u300830. Sims AC\u3001Burkett SE\u3001Yount B\u3001Pickles RJ (2008) SARS-CoV \u5728\u4eba\u985e\u50b3\u5c0e\u6c23\u9053\u4e0a\u76ae\u9ad4\u5916\u6a21\u578b\u4e2d\u7684\u8907\u5236\u548c\u767c\u75c5\u6a5f\u5236\u3002\u75c5\u6bd2\u7814\u7a76 133\uff1a33-44\u3002\u3009View Article\u3000Google Scholar 31.Xu X, Liu Y, Weiss S, Arnold E, Sarafianos SG, et al. (2003) Molecular model of SARS coronavirus polymerase: implications for biochemical functions and drug design. Nucleic Acids Res 31: 7117\u20137130.\u00a0\u00a0\u00a0 \u300831. Xu X\u3001Liu Y\u3001Weiss S\u3001Arnold E\u3001Sarafianos SG \u7b49\u3002 (2003) SARS \u51a0\u72c0\u75c5\u6bd2\u805a\u5408\u9176\u7684\u5206\u5b50\u6a21\u578b\uff1a\u5c0d\u751f\u5316\u529f\u80fd\u548c\u85e5\u7269\u8a2d\u8a08\u7684\u5f71\u97ff\u3002\u6838\u9178\u7814\u7a76 31\uff1a7117-7130\u3002\u3009View Article\u3000Google Scholar 32.Zhai Y, Sun F, Li X, Pang H, Xu X, et al. (2005) Insights into SARS-CoV transcription and replication from the structure of the nsp7-nsp8 hexadecamer. Nat Struct Mol Biol 12: 980\u2013986.\u00a0\u00a0\u00a0 \u300832. \u7fdf\u6bc5\u3001\u5b6b\u98db\u3001\u674e\u65ed\u3001\u9f90\u83ef\u3001\u5f90\u65ed\u7b49\u3002 (2005) \u5f9e nsp7-nsp8 \u5341\u516d\u805a\u9ad4\u7684\u7d50\u69cb\u6d1e\u5bdf SARS-CoV \u8f49\u9304\u548c\u5fa9\u5236\u3002 Nat Struct Mol Biol 12\uff1a980-986\u3002\u3009View Article\u3000Google Scholar 33.Stockman LJ, Bellamy R, Garner P (2006) SARS: Systematic Review of Treatment Effects. PLoS Med 3: e343.\u00a0\u00a0\u00a0 \u300833. Stockman LJ\u3001Bellamy R\u3001Garner P (2006) SARS\uff1a\u6cbb\u7642\u6548\u679c\u7684\u7cfb\u7d71\u8a55\u50f9\u3002 PLoS Med 3: e343\u3002\u3009View Article\u3000Google Scholar 34.Thompson A, Patel K, Tillman H, McHutchison JG (2009) Directly acting antivirals for the treatment of patients with hepatitis C infection: A clinical development update addressing key future challenges. J Hepatol 50: 184\u2013194.\u00a0\u00a0\u00a0 \u300834. Thompson A\u3001Patel K\u3001Tillman H\u3001McHutchison JG (2009) \u7528\u65bc\u6cbb\u7642\u4e19\u578b\u809d\u708e\u611f\u67d3\u60a3\u8005\u7684\u76f4\u63a5\u6297\u75c5\u6bd2\u85e5\u7269\uff1a\u61c9\u5c0d\u672a\u4f86\u95dc\u9375\u6311\u6230\u7684\u81e8\u5e8a\u958b\u767c\u66f4\u65b0\u3002 J Hepatol 50\uff1a184-194\u3002\u3009View Article\u3000Google Scholar 35.De Clercq E (2004) Antivirals and antiviral strategies. Nat Rev Microbiol 2: 704\u2013720.\u00a0\u00a0\u00a0 \u300835. De Clercq E (2004) \u6297\u75c5\u6bd2\u85e5\u7269\u548c\u6297\u75c5\u6bd2\u7b56\u7565\u3002 Nat Rev Microbiol 2: 704\u2013720\u3002\u3009View Article\u3000Google Scholar 36.Thompson AJV, McHutchison JG (2009) Antiviral resistance and specifically targeted therapy for HCV (STAT-C). J Viral Hepat 16: 377\u2013387.\u00a0\u00a0\u00a0 \u300836. Thompson AJV, McHutchison JG (2009) HCV \u6297\u75c5\u6bd2\u8010\u85e5\u6027\u548c\u7279\u7570\u6027\u9776\u5411\u6cbb\u7642 (STAT-C)\u3002 J \u75c5\u6bd2\u6027\u809d\u75c5 16\uff1a377-387\u3002\u3009View Article\u3000Google Scholar 37.Yang W, Lee JY, Nowotny M (2006) Making and Breaking Nucleic Acids: Two-Mg2+-Ion Catalysis and Substrate Specificity. Mol Cell 22: 5\u201313.\u00a0\u00a0\u00a0 \u300837. Yang W\u3001Lee JY\u3001Nowotny M (2006) \u88fd\u9020\u548c\u7834\u58de\u6838\u9178\uff1a\u96d9 Mg2+ \u96e2\u5b50\u50ac\u5316\u548c\u5e95\u7269\u7279\u7570\u6027\u3002\u6469\u723e\u7d30\u80de 22\uff1a5-13\u3002\u3009View Article\u3000Google Scholar 38.Castro C, Smidansky E, Maksimchuk KR, Arnold JJ, Korneeva VS, et al. (2007) Two proton transfers in the transition state for nucleotidyl transfer catalyzed by RNA- and DNA-dependent RNA and DNA polymerases. Proc Natl Acad Sci USA 104: 4267\u20134272.\u00a0\u00a0\u00a0 \u300838. Castro C\u3001Smidansky E\u3001Maksimchuk KR\u3001Arnold JJ\u3001Korneeva VS \u7b49\u3002 (2007) \u7531 RNA \u548c DNA \u4f9d\u8cf4\u6027 RNA \u548c DNA \u805a\u5408\u9176\u50ac\u5316\u7684\u6838\u82f7\u9178\u8f49\u79fb\u7684\u5169\u500b\u904e\u6e21\u614b\u8cea\u5b50\u8f49\u79fb\u3002 Proc Natl Acad Sci USA 104\uff1a4267\u20134272\u3002\u3009View Article\u3000Google Scholar 39.Arnold JJ, Ghosh SK, Cameron CE (1999) Poliovirus RNA-dependent RNA Polymerase (3Dpol). Divalent cation modulation of primer, template and nucleotide selection. J Biol Chem 274: 37060\u201337069.\u00a0\u00a0\u00a0 \u300839. Arnold JJ\u3001Ghosh SK\u3001Cameron CE (1999) \u810a\u9ad3\u7070\u8cea\u708e\u75c5\u6bd2 RNA \u4f9d\u8cf4\u6027 RNA \u805a\u5408\u9176 (3Dpol)\u3002\u5f15\u7269\u3001\u6a21\u677f\u548c\u6838\u82f7\u9178\u9078\u64c7\u7684\u4e8c\u50f9\u967d\u96e2\u5b50\u8abf\u7bc0\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 274\uff1a37060-37069\u3002\u3009View Article\u3000Google Scholar 40.Iuchi S (2001) Three classes of C2H2 zinc finger proteins. Cell Mol Life Sci 58: 625\u2013635.\u00a0\u00a0\u00a0 \u300840. Iuchi S (2001) \u4e09\u985e C2H2 \u92c5\u6307\u86cb\u767d\u3002\u7d30\u80de\u5206\u5b50\u751f\u547d\u79d1\u5b78 58\uff1a625-635\u3002\u3009View Article\u3000Google Scholar 41.Gomis-Ruth XF (2009) Catalytic domain architecture of metzincin metalloproteases. J Biol Chem 284: 15353\u201315357.\u00a0\u00a0\u00a0 \u300841. Gomis-Ruth XF (2009) Metzincin \u91d1\u5c6c\u86cb\u767d\u9176\u7684\u50ac\u5316\u57df\u7d50\u69cb\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 284\uff1a15353\u201315357\u3002\u3009View Article\u3000Google Scholar 42.Yap TL, Xu T, Chen Y-L, Malet H, Egloff M-P, et al. (2007) Crystal Structure of the Dengue Virus RNA-Dependent RNA Polymerase Catalytic Domain at 1.85-Angstrom Resolution. J Virol 81: 4753\u20134765.\u00a0\u00a0\u00a0 \u300842. Yap TL\u3001Xu T\u3001Chen Y-L\u3001Malet H\u3001Egloff M-P \u7b49\u3002 (2007) \u5206\u8fa8\u7387\u70ba 1.85 \u57c3\u7684\u767b\u9769\u71b1\u75c5\u6bd2\u4f9d\u8cf4\u65bc RNA \u7684 RNA \u805a\u5408\u9176\u50ac\u5316\u7d50\u69cb\u57df\u7684\u6676\u9ad4\u7d50\u69cb\u3002 J Virol 81\uff1a4753\u20134765\u3002\u3009View Article\u3000Google Scholar 43.Winek CL, Buehler EV (1966) Intravenous toxicity of zinc pyridinethione and several zinc salts. Toxicol Appl Pharmacol 9: 296\u2013273.\u00a0\u00a0\u00a0 \u300843. Winek CL, Buehler EV (1966) \u5421\u5576\u786b\u916e\u92c5\u548c\u5e7e\u7a2e\u92c5\u9e7d\u7684\u975c\u8108\u6bd2\u6027\u3002 Toxicol Appl Pharmacol 9\uff1a296-273\u3002\u3009View Article\u3000Google Scholar 44.Snyder DR, de Jesus CP, Towfighi J, Jacoby RO, Wedig JH (1979) Neurological, microscopic and enzyme-histochemical assessment of zinc pyrithione toxicity. Food Cosmet Toxicol 17: 651\u2013660.\u00a0\u00a0\u00a0 \u300844. Snyder DR, de Jesus CP, Towfighi J, Jacoby RO, Wedig JH (1979) \u5421\u5576\u786b\u916e\u92c5\u6bd2\u6027\u7684\u795e\u7d93\u5b78\u3001\u986f\u5fae\u93e1\u548c\u9176\u7d44\u7e54\u5316\u5b78\u8a55\u4f30\u3002\u98df\u54c1\u5316\u599d\u54c1\u6bd2\u7406\u5b78 17\uff1a651-660\u3002\u3009View Article\u3000Google Scholar 45.Magda D, Lecane P, Wang Z, Hu W, Thiemann P, et al. (2008) Synthesis and anticancer properties of water-soluble zinc ionophores. Cancer Res 68: 5318\u20135325.\u00a0\u00a0\u00a0 \u300845. Magda D\u3001Lecane P\u3001Wang Z\u3001Hu W\u3001Thiemann P \u7b49\u3002 (2008) \u6c34\u6eb6\u6027\u92c5\u96e2\u5b50\u8f09\u9ad4\u7684\u5408\u6210\u548c\u6297\u764c\u7279\u6027\u3002\u764c\u75c7\u7814\u7a76 68\uff1a5318-5325\u3002\u3009View Article\u3000oogle Scholar 46.Snijder EJ, van der Meer Y, Zevenhoven-Dobbe J, Onderwater JJM, van der Meulen J, et al. (2006) Ultrastructure and Origin of Membrane Vesicles Associated with the Severe Acute Respiratory Syndrome Coronavirus Replication Complex. J Virol 80: 5927\u20135940.\u00a0\u00a0\u00a0 \u300846. Snijder EJ\u3001van der Meer Y\u3001Zevenhoven-Dobbe J\u3001Onderwater JJM\u3001van der Meulen J \u7b49\u3002 (2006) \u8207\u56b4\u91cd\u6025\u6027\u547c\u5438\u7cfb\u7d71\u7d9c\u5408\u75c7\u51a0\u72c0\u75c5\u6bd2\u8907\u88fd\u8907\u5408\u9ad4\u76f8\u95dc\u7684\u819c\u56ca\u6ce1\u7684\u8d85\u5fae\u7d50\u69cb\u548c\u8d77\u6e90\u3002 J Virol 80\uff1a5927-5940\u3002\u3009View Article\u3000Google Scholar 47.Studier F (2005) Protein production by auto-induction in high density shaking cultures. Protein Expr Purif 41: 207\u2013234.\u00a0\u00a0\u00a0 \u300847. Studier F (2005) \u5728\u9ad8\u5bc6\u5ea6\u632f\u76ea\u57f9\u990a\u4e2d\u901a\u904e\u81ea\u52d5\u8a98\u5c0e\u7522\u751f\u86cb\u767d\u8cea\u3002\u86cb\u767d\u8cea Expr Purif 41\uff1a207-234\u3002\u3009View Article\u3000Google Scholar   \u91cd\u5fb7\u4e26\u4e14\u5c07\u5728\u5317\u4eac\u7684\u300c\u4e2d\u570b\u79d1\u5b78\u9662\u751f\u7269\u7269\u7406\u7814\u7a76\u6240\u611f\u67d3\u8207\u514d\u75ab\u5b78\u91cd\u9ede\u5be6\u9a57\u5ba4\u300d\u5728\u91ab\u5b78\u96dc\u8a8c\u4e0a\u6240\u767c\u8868\u7684\u4e00\u7bc7\u82f1\u6587\u7814\u7a76\u5831\u544a\u300a\u92c5\u6307\u6297\u75c5\u6bd2\u86cb\u767d\u6291\u5236\u7532\u578b\u6d41\u611f\u75c5\u6bd2\u86cb\u767d\u8868\u9054\u4ee5\u53ca\u88ab\u75c5\u6bd2\u7de8\u78bcNS1 \u5c0d\u6297\u7684\u7c21\u8981\u5f62\u5f0f\u300b\u7684\u4e2d\u6587\u81ea\u8b6f\u7248\u4e5f\u5bc4\u4e0a\u7d66\u4ed6\u53c3\u8003(\u5982\u9644\u6a94)\u3002 \u4e26\u4e14\uff0c\u91cd\u5fb7\u9084\u767c\u53bb\u4ee5\u4e0b\u5716\u7247\u8207\u8aaa\u660e\uff1a   \u300e\u9019\u5c31\u662f\u92c5\u6307\u7d50\u69cb\u5716\uff0c\u7531\u65bc\u9019\u662f\u87af\u5408\u7d50\u69cb\uff0c\u56e0\u6b64\u5b83\u80fd\u5f88\u5bb9\u6613\u5c07\u92c5\u96e2\u5b50\u7a7f\u900f\u7d30\u80de\u58c1\u800c\u8207\u8eb2\u85cf\u5728\u7d30\u80de\u5167\u840c\u767c\u7684\u75c5\u6bd2\u56e0\u70ba\u767c\u751f\u6291\u5236\u7ffb\u8b6f\u548c\u4fc3\u9032\u75c5\u6bd2 mRNA\u7684\u964d\u89e3\uff0c\u56e0\u6b64\u901a\u904e PARP \u7d50\u69cb\u57df\u800c\u4fc3\u9032 IAV \u86cb\u767d\u964d\u89e3\uff0c\u5be6\u9a57\u5df2\u7d93\u8b49\u5be6\u5b83\u53ef\u8b93\u5165\u4fb5\u5230\u7d30\u80de\u5167\u7684\u75c5\u6bd2\u4e4bRNA\u6a5f\u8f49\u767c\u751f\u4f11\u514b(\u4f46\u4e0d\u4e00\u5b9a\u6703\u6b7b\u4ea1)\uff0c\u800c\u4f7f\u5f97\u539f\u672c\u7121\u751f\u547d\u7684\u75c5\u6bd2\u56e0\u6b64\u4e0d\u80fd\u5927\u91cf\u8907\u88fd\u840c\u751f\uff0c\u800c\u53ef\u4ee5\u70ba\u4eba\u9ad4\u7684\u514d\u75ab\u7cfb\u7d71\u89e3\u6c7a\u3002\u300f \u9019\u4e00\u4f4d\u738b\u59d3\u7db2\u53cb\u53c8\u767c\u4f86\u4ee5\u4e0b\u4fe1\u606f\uff1a\u300e\u80fd\u5920\u6bba\u75c5\u6bd2\u800c\u4e0d\u6703\u6bba\u6b7b\u6211\u5011\u81ea\u5df1\u7684\u7d30\u80de,\u5929\u4e0b\u6709\u9019\u9ebc\u597d\u7684\u4e8b\u55ce\uff1f\u4e0d\u904e\u662f\u71df\u990a\u88dc\u52a9\u54c1\u800c\u5df2\uff01\u300f \u91cd\u5fb7\u770b\u4ed6\u5728\u610f\u6c23\u7528\u4e8b\uff0c\u56e0\u6b64\u5c31\u56de\u8986\u4ed6\u8aaa\uff1a\u300e\u96a8\u60a8\u9ad8\u8208\u600e\u9ebc\u8aaa\u90fd\u597d\uff0c\u6211\u6c92\u6709\u8aaa\u5b83\u662f\u85e5\uff0c\u53ea\u8aaa\u662f\u4fdd\u5065\u54c1\u800c\u5df2\u3002\u300f\u767c\u5b8c\u4e86\u9019\u4fe1\u606f\u4e4b\u5f8c\uff0c\u91cd\u5fb7\u767c\u8a93\u4ee5\u5f8c\u518d\u4e5f\u4e0d\u5c0d\u9019\u7a2e\u96de\u86cb\u88e1\u6311\u9aa8\u982d\u7684\u4eba\u63d0\u4f9b\u4efb\u4f55\u6709\u50f9\u503c\u7684\u8cc7\u8a0a\u4e86\u3002 \u4f46\u662f\uff0c\u91cd\u5fb7\u9084\u662f\u8981\u544a\u8a34\u5927\u5bb6\u70ba\u4f55\u8981\u5efa\u8b70\u5927\u5bb6\u5403\u542b\u92c5\u96e2\u5b50\u7684\u4fdd\u5065\u98df\u54c1\uff0c\u7576\u7136\u92c5\u5f9e\u98df\u7269\u4e2d\u651d\u53d6\u4e5f\u6709\uff0c\u4f46\u662f\u98df\u7269\u5fc5\u9808\u7d93\u904e\u6d88\u5316\u5f8c\u92c5\u96e2\u5b50\u624d\u6703\u900f\u904e\u8840\u6db2\u800c\u5230\u4eba\u9ad4\u5668\u5b98\uff0c\u9019\u500b\u904e\u7a0b\u592a\u9577\u592a\u4e45\u4e86\uff0c\u56e0\u6b64\u92c5\u96e2\u5b50\u53ef\u80fd\u53c8\u8207\u5176\u4ed6\u7269\u8cea\u7d50\u5408\u6210\u5316\u5408\u7269\uff0c\u800c\u518d\u4e0d\u80fd\u767c\u751f\u8207\u8ca0\u96e2\u5b50\u7684\u75c5\u6bd2\u4e2d\u548c\u7684\u6548\u679c\u3002\u56e0\u6b64\u5c31\u66f4\u6c92\u6709\u767c\u63ee\u5230\u92c5\u96e2\u5b50\u6240\u7368\u5177\u7684\u87af\u5408\u4ea4\u63db\u6548\u679c\u3002 \u7531\u65bc\uff0c\u611f\u5192\u6216\u662fCOVID-19\u7684\u521d\u671f\u75c5\u72c0\u4e3b\u8981\u90fd\u662f\u91dd\u5c0d\u4e0a\u652f\u6c23\u7ba1\uff0c\u56e0\u6b64\u4ee3\u8868\u547c\u5438\u7ba1\u9053\u7684\u75c5\u6bd2\u91cf\u6700\u5927\uff0c\u56e0\u6b64\u53ef\u4ee5\u542b\u5728\u5634\u4e2d\u6162\u6162\u5206\u89e3\u7684\u5589\u7cd6\u4e4b\u6548\u679c\u6700\u597d\uff0c\u56e0\u70ba\u5b83\u8b93\u901a\u904e\u7684\u6c23\u6d41\u4e2d\u7684\u75c5\u6bd2\u5148\u88ab\u92c5\u96e2\u5b50\u4e2d\u548c\uff0c\u800c\u7cd6\u6c41\u6d41\u5165\u98df\u9053\u6642\u92c5\u96e2\u5b50\u4e5f\u6703\u6ea2\u5165\u4e0a\u652f\u6c23\u7ba1\u3002\u9019\u5c31\u662f\u70ba\u4f55\u91cd\u5fb7\u9996\u5148\u63a8\u85a6\u5df2\u7d93\u5728\u7f8e\u570b\u98a8\u884c30\u591a\u5e74\u7684\u611f\u5192\u9810\u9632\u7528\u85e5Cold Eeze\u4e2d\u6587\u8b6f\u540d\u70ba\u3010\u92c5\u7cd6\u3011\uff0c\u56e0\u70ba\u5b83\u5df2\u7d93\u904e\u4e86\u5c08\u5229\u4fdd\u8b77\u671f\uff0c\u5b78\u540d\u85e5\u540d\u662fGeneric Version\uff0c\u56e0\u70ba\u552e\u50f9\u8f03\u4fbf\u5b9c(\u4f46\u662f\u73fe\u5728\u5168\u7403\u6436\u8cfc\u5f88\u53ef\u80fd\u7f3a\u8ca8)\u3002 \u73fe\u5728\uff0c\u91cd\u5fb7\u8981\u544a\u8a34\u5927\u5bb6\u4e00\u500b\u79d8\u5bc6\uff0c\u90a3\u5c31\u662f\u70ba\u4f55\u91cd\u5fb7\u8981\u5c0d\u92c5\u96e2\u5b50\u9032\u884c\u7814\u7a76\u5462\uff0c\u6545\u4e8b\u662f\u9019\u6a23\u5b50\u7684\uff0c\u7531\u65bc\u4e00\u5e74\u524d \u91cd\u5fb7 \u8acb\u793a\u201c\u9748\u7236\u201d(\u5143\u59cb\u5929\u5c0a)\u8aaa\uff1a\u300e\u65e2\u7136\u00a0 \u9748\u7236\u80fd\u63d0\u524d\u534a\u5e74\u8b66\u544a\u6211\u5011\u5c07\u6709\u6b64\u5927\u75ab\u60c5\uff0c\u662f\u5426\u80fd\u544a\u77e5\u5f92\u5152\u5982\u4f55\u88fd\u5f97\u89e3\u85e5\uff1f\u300f\u64f2\u832d\u61c9\u8a31\u5f8c\uff0c\u91cd\u5fb7 \u6b63\u4e0d\u77e5\u5982\u4f55\u518d\u554f\u9019\u89e3\u85e5\u79d8\u65b9\u6642\uff0c\u8166\u4e2d\u7a81\u7136\u6e67\u73fe\u4e00\u500b\u201c\u92c5\u201d\u5b57\uff0c\u64f2\u832d\u518d\u554f\u78ba\u5b9a\u795e\u8aed\u5c31\u662f\u6b64\u201c\u92c5\u201d\u5b57\u7121\u8aa4\u3002 \u5728\u6b64\u4e4b\u5f8c\uff0c\u91cd\u5fb7\u624d\u958b\u59cb\u8a73\u95b1\u5404\u7a2e\u82f1\u6587\u91ab\u5b78\u6587\u737b\uff0c\u8d8a\u767c\u8b49\u5be6\u92c5\u96e2\u5b50\u6709\u6291\u5236mRNA\u75c5\u6bd2\u7684\u6548\u679c\uff0c\u800c\u4e14\u56e0\u70ba\u92c5\u96e2\u5b50\u662f\u63a1\u53d6\u87af\u5408\u65b9\u5f0f\u8b93\u92c5\u96e2\u5b50\u7a7f\u900f\u7d30\u80de\u58c1\u9032\u5165\u4f86\u964d\u89e3\u75c5\u6bd2\u86cb\u767d\u8907\u88fd\u6240\u9700\u6c42\u7684PARP \u7d50\u69cb\u57df\uff0c\u56e0\u6b64\u9019\u662f\u4e00\u7a2e\u7cbe\u6e96\u7834\u7a97\u5f0f\u7684\u65ac\u9996\u884c\u52d5\uff0c\u5c0d\u65bc\u7d30\u80de\u7684\u50b7\u5bb3\u751a\u5c0f\uff0c\u56e0\u6b64\u5c0d\u65bc\u4eba\u9ad4\u7684\u5371\u5bb3\u751a\u4f4e\u3002 \u91cd\u5fb7\u4e00\u5e74\u524d\u5c31\u628a\u7814\u7a76\u6210\u679c\u516c\u958b\uff0c\u4e00\u4f86\u56e0\u70ba\u81ea\u5df1\u962e\u56ca\u7f9e\u6f80\uff0c\u7121\u6cd5\u7e7c\u7e8c\u7814\u767c\uff0c\u4e8c\u4f86\u91cd\u5fb7\u5df2\u7d93\u9810\u7aba\u5230\u672a\u4f86\u9084\u6709\u5176\u4ed6\u56b4\u91cd\u75be\u75c5\uff0c\u800c\u4e14COVID-19\u9084\u6703\u5927\u7a81\u8b8a\uff0c\u56e0\u6b64\u8b93\u5176\u4ed6\u4eba\u63a5\u68d2\u4f86\u7814\u7a76\u6216\u8a31\u6703\u66f4\u6709\u6210\u6548\u3002","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/pingtungtimes.com.tw\/?p=104514","og_locale":"zh_TW","og_type":"article","og_title":"\u597d\u201c\u92c5\u201d\u3000\u4e5f\u6703\u60f9\u4e86\u4e00\u8eab\u8165 - \u5c4f\u6771\u6642\u5831\u96fb\u5b50\u5831","og_description":"\u3010\u674e\u91cd\u5fb7\uff0f\u5c08\u6b04\u5831\u5c0e2022\/05\/27\u3011\u3000\u4eca\u65e9\uff0c\u6709\u4e00\u78ba\u8a3a\u7652\u5f8c\u8005\u8207\u91cd\u5fb7\u8a0e\u8ad6\u4ed6\u7684\u75c5\u60c5\uff0c\u91cd\u5fb7\u5efa\u8b70\u4ed6\u88dc\u5145\u92c5\u96e2\u5b50\u98df\u54c1\uff0c\u4e26\u5c07\u6b64\u4e00\u4fe1\u606f\u4e5f\u767c\u7d66\u5927\u5bb6\u77e5\u66c9\uff1a \u300e\u6709\u78ba\u8a3a\u7652\u5f8c\u8005\u554f\u91cd\u5fb7\u8981\u5982\u4f55\u4fdd\u5065\u4ee5\u9632\u5fa9\u967d\uff0c\u91cd\u5fb7 \u7686\u5efa\u8b70\u4ed6\u5403\u3010\u92c5\u7cd6\u3011(\u985e\u4f3c\u5589\u7247)\u5c0f\u5b69\u5247\u5403\u3010\u5152\u7ae5\u92c5\u8edf\u7cd6\u3011\uff0c\u6216\u662f\u3010\u9175\u6bcd\u92c5\u3011\u3001\u3010\u87af\u5408\u92c5\u3011\u6216\u662f\u3010\u8461\u8404\u7cd6\u9178\u92c5\u3011\u4f86\u6d88\u6ec5\u6f5b\u85cf\u4eba\u9ad4\u5c1a\u672a\u767c\u4f5c\u7684\u75c5\u6bd2\u3002\u300f \u5f8c\u4f86\u5c31\u63a5\u7372\u4e00\u4f4d\u738b\u59d3\u7db2\u53cb\u56de\u8986\u8aaa\uff1a\u300e\u4e0d\u5c0d\u5594\uff01\u92c5,\u4eba\u985e\u5b58\u6d3b\u6240\u9700\u8981\u7684\u5143\u7d20\u4e4b\u4e00,\u5f9e\u5929\u7136\u98df\u7269\u76f4\u63a5\u651d\u53d6\u5c31\u8db3\u5920\u4e86,\u4eba\u70ba\u8d85\u91cf\u7684\u88dc\u5145\u6703\u51fa\u554f\u984c\u7684\uff01\u92c5,\u4e0d\u6703\u76f4\u63a5\u6bba\u75c5\u6bd2\u7684\uff01\u300f \u9019\u4e00\u4f4d\u738b\u5148\u751f\uff0c\u5927\u6982\u6f0f\u770b\u4e86\u91cd\u5fb7\u5728\u4e00\u5e74\u524d\u5728\u672c\u6703\u7fa4\u7d44\u4e0a\u767c\u8868\u7684\u5e7e\u7bc7\u4ee5\u92c5\u96e2\u5b50\u4f86\u6d88\u6ec5\u75c5\u6bd2\u7684\u6587\u7ae0\uff0c\u56e0\u6b64\uff0c\u91cd\u5fb7\u5c31\u56de\u8986\u4ed6\u8aaa\uff1a\u300e\u6211\u770b\u904e\u4e86\u8a31\u591a\u82f1\u6587\u91ab\u5b78\u6587\u737b\uff0c\u4e5f\u8207\u9ec3\u25cb\u5bcc\u535a\u58eb\u548c\u9673\u25cb\u5e73\u91ab\u5e2b\u8a0e\u8ad6\u904e\u3002\u60a8\u5982\u679c\u60f3\u8981\u770b\u9019\u4e00\u4e9b\u82f1\u6587\u91ab\u5b78\u6587\u737b\uff0c\u6211\u53ef\u4ee5\u5bc4\u53bb\u7d66\u60a8\u3002\u300f\u7136\u5f8c \u91cd\u5fb7 \u5c31\u5c31\u5bc4\u53bb\u7d66\u4ed6\u4ee5\u4e0b\u7684\u8cc7\u6599\uff1a References\u53c3\u8003\u6587\u737b 1.Lazarczyk M, Favre M (2008) Role of Zn2+ ions in host-virus interactions. J Virol 82: 11486\u201311494.\u00a0\u00a0\u00a0 \u30081. Lazarczyk M, Favre M (2008) Zn2+ \u96e2\u5b50\u5728\u5bbf\u4e3b-\u75c5\u6bd2\u76f8\u4e92\u4f5c\u7528\u4e2d\u7684\u4f5c\u7528\u3002 J Virol 82\uff1a11486\u201311494\u3002\u3009\u3000View Article\u3000Google Scholar 2.Frederickson CJ, Koh JY, Bush AI (2005) Neurobiology of zinc in health and disease. Nat Rev Neurosci 6: 449\u2013462.\u00a0\u00a0\u00a0 \u30082. Frederickson CJ\u3001Koh JY\u3001Bush AI (2005) \u92c5\u5728\u5065\u5eb7\u548c\u75be\u75c5\u4e2d\u7684\u795e\u7d93\u751f\u7269\u5b78\u3002 Nat Rev Neurosci 6\uff1a449-462\u3002\u3009View Article\u3000Google Scholar 3.Alirezaei M, Nairn AC, Glowinski J, Premont J, Marin P (1999) Zinc inhibits protein synthesis in neurons: potential rol of phosphorylation of translation initiation factor-2a. J Biol Chem 274: 32433\u201332438.\u00a0\u00a0\u00a0 \u30083. Alirezaei M\u3001Nairn AC\u3001Glowinski J\u3001Premont J\u3001Marin P (1999) \u92c5\u6291\u5236\u795e\u7d93\u5143\u4e2d\u7684\u86cb\u767d\u8cea\u5408\u6210\uff1a\u7ffb\u8b6f\u8d77\u59cb\u56e0\u5b50 2a \u78f7\u9178\u5316\u7684\u6f5b\u5728\u4f5c\u7528\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 274\uff1a32433\u201332438\u3002\u3009View Article\u3000Google Scholar 4.Uchide N, Ohyama K, Bessho T, Yuan B, Yamakawa T (2002) Effect of antioxidants on apoptosis induced by influenza virus infection: inhibition of viral gene replication and transcription with pyrrolidine dithiocarbamate. Antiviral Res 56: 207\u2013217.\u00a0\u00a0\u00a0 \u30084. Uchide N, Ohyama K, Bessho T, Yuan B, Yamakawa T (2002) \u6297\u6c27\u5316\u5291\u5c0d\u6d41\u611f\u75c5\u6bd2\u611f\u67d3\u8a98\u5c0e\u7684\u7d30\u80de\u51cb\u4ea1\u7684\u5f71\u97ff\uff1a\u7528\u5421\u54af\u70f7\u4e8c\u786b\u4ee3\u6c28\u57fa\u7532\u9178\u916f\u6291\u5236\u75c5\u6bd2\u57fa\u56e0\u8907\u5236\u548c\u8f49\u9304\u3002\u6297\u75c5\u6bd2\u7814\u7a76 56\uff1a207-217\u3002\u3009View Article\u3000Google Scholar 5.Suara RO, Crowe JEJ (2004) Effect of zinc salts on respiratory syncytial virus replication. Antimicrob Agents Chemother 48: 783\u2013790.\u00a0\u00a0\u00a0 \u30085. Suara RO, Crowe JEJ (2004) \u92c5\u9e7d\u5c0d\u547c\u5438\u9053\u5408\u80de\u75c5\u6bd2\u8907\u88fd\u7684\u5f71\u97ff\u3002\u6297\u5fae\u751f\u7269\u5291 Chemother 48\uff1a783-790\u3002\u3009View Article\u3000Google Scholar 6.Gaudernak E, Seipelt J, Triendl A, Grassauer A, Kuechler E (2002) Antiviral Effects of Pyrrolidine Dithiocarbamate on Human Rhinoviruses. J Virol 76: 6004\u20136015.\u00a0\u00a0\u00a0 \u30086. Gaudernak E\u3001Seipelt J\u3001Triendl A\u3001Grassauer A\u3001Kuechler E (2002) \u5421\u54af\u70f7\u4e8c\u786b\u4ee3\u6c28\u57fa\u7532\u9178\u916f\u5c0d\u4eba\u9f3b\u75c5\u6bd2\u7684\u6297\u75c5\u6bd2\u4f5c\u7528\u3002 J Virol 76\uff1a6004-6015\u3002\u3009View Article\u3000Google Scholar 7.Si X, McManus BM, Zhang J, Yuan J, Cheung C, et al. (2005) Pyrrolidine Dithiocarbamate Reduces Coxsackievirus B3 Replication through Inhibition of the Ubiquitin-Proteasome Pathway. J Virol 79: 8014\u20138023.\u00a0\u00a0\u00a0 \u30087. Si X, McManus BM, Zhang J, Yuan J, Cheung C, et al. (2005) Pyrrolidine Dithiocarbamate \u901a\u904e\u6291\u5236\u6cdb\u7d20-\u86cb\u767d\u9176\u9ad4\u9014\u5f91\u6e1b\u5c11\u67ef\u85a9\u5947\u75c5\u6bd2 B3 \u8907\u88fd\u3002 J Virol 79\uff1a8014-8023\u3002\u3009View Article\u3000Google Scholar 8.Korant BD, Kauer JC, Butterworth BE (1974) Zinc ions inhibit replication of rhinoviruses. Nature 248: 588\u2013590.\u00a0\u00a0\u00a0 \u30088. Korant BD, Kauer JC, Butterworth BE (1974) \u92c5\u96e2\u5b50\u6291\u5236\u9f3b\u75c5\u6bd2\u7684\u8907\u5236\u3002\u81ea\u7136 248\uff1a588-590\u3002\u3009View Article\u3000Google Scholar 9.Polatnick J, Bachrach HL (1978) Effect of zinc and other chemical agents on foot-and-mouth-disease virus replication. Antimicrob Agents Chemother 13: 731\u2013734.\u00a0\u00a0 \u00a0\u30089. Polatnick J, Bachrach HL (1978) \u92c5\u548c\u5176\u4ed6\u5316\u5b78\u8a66\u5291\u5c0d\u53e3\u8e44\u75ab\u75c5\u6bd2\u8907\u88fd\u7684\u5f71\u97ff\u3002\u6297\u5fae\u751f\u7269\u5291 Chemother 13\uff1a731-734\u3002\u3009View Article\u3000Google Scholar 10.Lanke K, Krenn BM, Melchers WJG, Seipelt J, van Kuppeveld FJM (2007) PDTC inhibits picornavirus polyprotein processing and RNA replication by transporting zinc ions into cells. J Gen Virol 88: 1206\u20131217.\u00a0\u00a0\u00a0 \u300810. Lanke K\u3001Krenn BM\u3001Melchers WJG\u3001Seipelt J\u3001van Kuppeveld FJM (2007) PDTC \u901a\u904e\u5c07\u92c5\u96e2\u5b50\u8f49\u904b\u5230\u7d30\u80de\u4e2d\u4f86\u6291\u5236\u5c0f\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u591a\u86cb\u767d\u52a0\u5de5\u548c RNA \u8907\u88fd\u3002 J Gen Virol 88\uff1a1206-1217\u3002\u3009View Article\u3000Google Scholar7 11.Krenn BM, Gaudernak E, Holzer B, Lanke K, Van Kuppeveld FJM, et al. (2009) Antiviral Activity of the Zinc Ionophores Pyrithione and Hinokitiol against Picornavirus Infections. J Virol 83: 58\u201364.\u00a0\u00a0\u00a0 \u300811. Krenn BM\u3001Gaudernak E\u3001Holzer B\u3001Lanke K\u3001Van Kuppeveld FJM \u7b49\u3002 (2009) \u92c5\u96e2\u5b50\u8f09\u9ad4\u5421\u5576\u786b\u916e\u548c\u6a9c\u9187\u5c0d\u5c0f\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u611f\u67d3\u7684\u6297\u75c5\u6bd2\u6d3b\u6027\u3002 J Virol 83\uff1a58-64\u3002\u3009View Article\u3000Google Scholar 12.Zalewski PD, Forbes IJ, Betts WH (1993) Correlation of apoptosis with change in intracellular labile Zn(II) using Zinquin , a new specific fluorescent probe for Zn(II). Biochem J 296: 403\u2013408.\u00a0 \u300812. Zalewski PD, Forbes IJ, Betts WH (1993) \u4f7f\u7528 Zinquin \uff0c\u7d30\u80de\u51cb\u4ea1\u8207\u7d30\u80de\u5167\u4e0d\u7a69\u5b9a\u92c5\uff08II\uff09\u8b8a\u5316\u7684\u76f8\u95dc\u6027\uff0c\u4e00\u7a2e\u65b0\u7684\u7279\u7570\u6027Zn(II) \u7684\u7192\u5149\u63a2\u91dd\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 296\uff1a403-408\u3002\u3009View Article\u3000Google Scholar 13.Baum EZ, Bebernitz GA, Palant O, Mueller T, Plotch SJ (1991) Purification, properties, and mutagenesis of poliovirus 3C protease. Virology 165: 140\u2013150.\u00a0\u00a0\u00a0 \u300813. Baum EZ\u3001Bebernitz GA\u3001Palant O\u3001Mueller T\u3001Plotch SJ (1991) \u810a\u9ad3\u7070\u8cea\u708e\u75c5\u6bd2 3C \u86cb\u767d\u9176\u7684\u7d14\u5316\u3001\u7279\u6027\u548c\u8a98\u8b8a\u3002\u75c5\u6bd2\u5b78 165\uff1a140-150\u3002\u3009View Article\u3000Google Scholar 14.Cordingley MG, Register RB, Callahan PL, Garsky VM, Colonno RJ (1989) Cleavage of small peptides in vitro by human rhinovirus 14 3C protease expressed in Escherichia coli. J Virol 63: 5037\u20135045.\u00a0\u00a0\u00a0 \u300814. Cordingley MG\u3001Register RB\u3001Callahan PL\u3001Garsky VM\u3001Colonno RJ (1989) \u5728\u5927\u8178\u687f\u83cc\u4e2d\u8868\u9054\u7684\u4eba\u9f3b\u75c5\u6bd2 14 3C \u86cb\u767d\u9176\u9ad4\u5916\u88c2\u89e3\u5c0f\u80bd\u3002 J Virol 63\uff1a5037-5045\u3002\u3009View Article\u3000Google Scholar 15.Ferrari E, Wright-Minogue J, Fang JW, Baroudy BM, Lau JY, et al. (1999) Characterization of soluble hepatitis C virus RNA-dependent RNA polymerase expressed in Escherichia coli. J Virol 73: 1649\u20131654.\u00a0\u00a0\u00a0 \u300815. Ferrari E\u3001Wright-Minogue J\u3001Fang JW\u3001Baroudy BM\u3001Lau JY \u7b49\u3002 (1999) \u5728\u5927\u8178\u687f\u83cc\u4e2d\u8868\u9054\u7684\u53ef\u6eb6\u6027\u4e19\u578b\u809d\u708e\u75c5\u6bd2 RNA \u4f9d\u8cf4\u6027 RNA \u805a\u5408\u9176\u7684\u8868\u5fb5\u3002 J Virol 73\uff1a1649-1654\u3002\u3009View Article\u3000Google Scholar 16.Hung M, Gibbs CS, Tsiang M (2002) Biochemical characterization of rhinovirus RNA-dependent RNA polymerase. Antiviral Res 56: 99\u2013114.\u00a0\u00a0\u00a0 \u300816. Hung M\u3001Gibbs CS\u3001Tsiang M (2002) \u9f3b\u75c5\u6bd2 RNA \u4f9d\u8cf4\u6027 RNA \u805a\u5408\u9176\u7684\u751f\u5316\u7279\u5fb5\u3002\u6297\u75c5\u6bd2\u7814\u7a76 56\uff1a99-114\u3002\u3009View Article\u3000Google Scholar 17.Perlman S, Netland J (2009) Coronaviruses post-SARS: update on replication and pathogenesis. Nat Rev Micro 7: 439\u2013450.\u00a0\u00a0\u00a0 \u300817. Perlman S, Netland J (2009) SARS \u5f8c\u7684\u51a0\u72c0\u75c5\u6bd2\uff1a\u8907\u88fd\u548c\u767c\u75c5\u6a5f\u5236\u7684\u66f4\u65b0\u3002 Nat Rev Micro 7\uff1a439-450\u3002\u3009View Article\u3000Google Scholar 18.Gorbalenya AE, Enjuanes L, Ziebuhr J, Snijder EJ (2006) Nidovirales: evolving the largest RNA virus genome. Virus Res 117: 17\u201337.\u00a0\u00a0\u00a0 \u300818. Gorbalenya AE\u3001Enjuanes L\u3001Ziebuhr J\u3001Snijder EJ (2006) Nidovirales\uff1a\u9032\u5316\u6700\u5927\u7684 RNA \u75c5\u6bd2\u57fa\u56e0\u7d44\u3002\u75c5\u6bd2\u7814\u7a76 117\uff1a17-37\u3002\u3009View Article\u3000Google Scholar 19.Snijder EJ, Bredenbeek PJ, Dobbe JC, Thiel V, Ziebuhr J, et al. (2003) Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage. J Mol Biol 331: 991\u20131004.\u00a0\u00a0\u00a0 \u300819. Snijder EJ\u3001Bredenbeek PJ\u3001Dobbe JC\u3001Thiel V\u3001Ziebuhr J \u7b49\u3002 (2003) SARS \u51a0\u72c0\u75c5\u6bd2\u57fa\u56e0\u7d44\u548c\u86cb\u767d\u8cea\u7d44\u7684\u7368\u7279\u548c\u4fdd\u5b88\u7279\u5fb5\uff0c\u5f9e\u51a0\u72c0\u75c5\u6bd2\u7d44 2 \u8b5c\u7cfb\u7684\u65e9\u671f\u5206\u88c2\u3002 J Mol Biol 331\uff1a991-1004\u3002\u3009View Article\u3000Google Scholar 20.Pasternak AO, Spaan WJ, Snijder EJ (2006) Nidovirus transcription: how to make sense\u2026? J Gen Virol 80: 1403\u20131421.\u00a0\u00a0\u00a0 \u300820. Pasternak AO\u3001Spaan WJ\u3001Snijder EJ (2006) Nidovirus \u8f49\u9304\uff1a\u5982\u4f55\u7406\u89e3\u2026\u2026\uff1f J Gen Virol 80\uff1a1403\u20131421\u3002\u3009View Article\u3000Google Scholar 21.Sawicki SG, Sawicki DL, Siddell SG (2007) A Contemporary View of Coronavirus Transcription. J Virol 81: 20\u201329.\u00a0\u00a0\u00a0 \u300821. Sawicki SG\u3001Sawicki DL\u3001Siddell SG\uff082007\uff09\u51a0\u72c0\u75c5\u6bd2\u8f49\u9304\u7684\u7576\u4ee3\u89c0\u9ede\u3002 J Virol 81\uff1a20-29\u3002\u3009View Article\u3000Google Scholar 22.Butterworth BE, Korant BD (1974) Characterization of the large picornaviral polypeptides produced in the presence of zinc ion. J Virol 14: 282\u2013291.\u00a0\u00a0\u00a0 \u300822. Butterworth BE, Korant BD (1974) \u5728\u92c5\u96e2\u5b50\u5b58\u5728\u4e0b\u7522\u751f\u7684\u5927\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u591a\u80bd\u7684\u8868\u5fb5\u3002 J Virol 14\uff1a282-291\u3002\u3009View Article\u3000Google Scholar 23.Denison MR, Perlman S (1986) Translation and processing of mouse hepatitis virus virion RNA in a cell-free system. J Virol 60: 12\u201318.\u00a0\u00a0\u00a0 \u300823. Denison MR, Perlman S (1986) \u5728\u7121\u7d30\u80de\u7cfb\u7d71\u4e2d\u7ffb\u8b6f\u548c\u8655\u7406\u5c0f\u9f20\u809d\u708e\u75c5\u6bd2\u75c5\u6bd2\u9ad4 RNA\u3002 J Virol 60\uff1a12-18\u3002\u3009View Article\u3000Google Scholar 24.Denison MR, Zoltick PW, Hughes SA, Giangreco B, Olson AL, et al. (1992) Intracellular processing of the N-terminal ORF 1a proteins of the coronavirus MHV-A59 requires multiple proteolytic events. Virology 189: 274\u2013284.\u00a0\u00a0\u00a0 \u300824. Denison MR\u3001Zoltick PW\u3001Hughes SA\u3001Giangreco B\u3001Olson AL \u7b49\u3002 (1992) \u51a0\u72c0\u75c5\u6bd2 MHV-A59 \u7684 N \u7aef ORF 1a \u86cb\u767d\u7684\u7d30\u80de\u5167\u52a0\u5de5\u9700\u8981\u591a\u500b\u86cb\u767d\u6c34\u89e3\u4e8b\u4ef6\u3002\u75c5\u6bd2\u5b78 189\uff1a274-284\u3002\u3009View Article\u3000Google Scholar 25.van Hemert MJ, de Wilde AH, Gorbalenya AE, Snijder EJ (2008) The in Vitro RNA Synthesizing Activity of the Isolated Arterivirus Replication\/Transcription Complex Is Dependent on a Host Factor. J Biol Chem 283: 16525\u201316536.\u00a0\u00a0\u00a0 \u300825. van Hemert MJ\u3001de Wilde AH\u3001Gorbalenya AE\u3001Snijder EJ (2008) \u5206\u96e2\u7684\u52d5\u8108\u75c5\u6bd2\u8907\u88fd\/\u8f49\u9304\u8907\u5408\u7269\u7684\u9ad4\u5916 RNA \u5408\u6210\u6d3b\u6027\u53d6\u6c7a\u65bc\u5bbf\u4e3b\u56e0\u5b50\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 283\uff1a16525-16536\u3002\u3009View Article\u3000Google Scholar 26.van Hemert MJ, van den Worm SHE, Knoops K, Mommaas AM, Gorbalenya AE, et al. (2008) SARS-Coronavirus Replication\/Transcription Complexes Are Membrane-Protected and Need a Host Factor for Activity In Vitro. PLoS Pathog 4: e1000054.\u00a0\u00a0\u00a0 \u300826. van Hemert MJ\u3001van den Worm SHE\u3001Knoops K\u3001Mommaas AM\u3001Gorbalenya AE \u7b49\u3002 (2008) SARS-\u51a0\u72c0\u75c5\u6bd2\u8907\u88fd\/\u8f49\u9304\u8907\u5408\u7269\u53d7\u819c\u4fdd\u8b77\uff0c\u9700\u8981\u5bbf\u4e3b\u56e0\u5b50\u624d\u80fd\u9032\u884c\u9ad4\u5916\u6d3b\u52d5\u3002 PLoS \u75c5\u539f\u9ad4 4\uff1ae1000054\u3002\u3009View Article\u3000Google Scholar 27.te Velthuis AJ, Arnold JJ, Cameron CE, van den Worm SH, Snijder EJ (2009) The RNA polymerase activity of SARS-coronavirus nsp12 is primer dependent. Nucleic Acids Res 38: 203\u2013214.\u00a0\u00a0\u00a0 \u300827. te Velthuis AJ\u3001Arnold JJ\u3001Cameron CE\u3001van den Worm SH\u3001Snijder EJ (2009) SARS \u51a0\u72c0\u75c5\u6bd2 nsp12 \u7684 RNA \u805a\u5408\u9176\u6d3b\u6027\u4f9d\u8cf4\u65bc\u5f15\u7269\u3002\u6838\u9178\u7814\u7a76 38\uff1a203-214\u3002\u3009View Article\u3000Google Scholar 28.Beerens N, Selisko B, Ricagno S, Imbert I, van der Zanden L, et al. (2007) De Novo Initiation of RNA Synthesis by the Arterivirus RNA-Dependent RNA Polymerase. J Virol 81: 8384\u20138395.\u00a0\u00a0\u00a0 \u300828. Beerens N\u3001Selisko B\u3001Ricagno S\u3001Imbert I\u3001van der Zanden L \u7b49\u3002 (2007) \u901a\u904e\u52d5\u8108\u75c5\u6bd2 RNA \u4f9d\u8cf4\u7684 RNA \u805a\u5408\u9176\u5f9e\u982d\u958b\u59cb\u5408\u6210 RNA\u3002 J Virol 81\uff1a8384\u20138395\u3002\u3009View Article\u3000Google Scholar 29.van den Born E, Posthuma CC, Knoops K, Snijder EJ (2007) An infectious recombinant equine arteritis virus expressing green fluorescent protein from its replicase gene. J Gen Virol 88: 1196\u20131205.\u00a0\u00a0\u00a0 \u300829. van den Born E, Posthuma CC, Knoops K, Snijder EJ (2007) \u4e00\u7a2e\u50b3\u67d3\u6027\u91cd\u7d44\u99ac\u52d5\u8108\u708e\u75c5\u6bd2\uff0c\u5f9e\u5176\u8907\u5236\u9176\u57fa\u56e0\u8868\u9054\u7da0\u8272\u7192\u5149\u86cb\u767d\u3002 J Gen Virol 88\uff1a1196-1205\u3002\u3009View Article\u3000Google Scholar 30.Sims AC, Burkett SE, Yount B, Pickles RJ (2008) SARS-CoV replication and pathogenesis in an in vitro model of the human conducting airway epithelium. Virus Res 133: 33\u201344.\u00a0\u00a0\u00a0 \u300830. Sims AC\u3001Burkett SE\u3001Yount B\u3001Pickles RJ (2008) SARS-CoV \u5728\u4eba\u985e\u50b3\u5c0e\u6c23\u9053\u4e0a\u76ae\u9ad4\u5916\u6a21\u578b\u4e2d\u7684\u8907\u5236\u548c\u767c\u75c5\u6a5f\u5236\u3002\u75c5\u6bd2\u7814\u7a76 133\uff1a33-44\u3002\u3009View Article\u3000Google Scholar 31.Xu X, Liu Y, Weiss S, Arnold E, Sarafianos SG, et al. (2003) Molecular model of SARS coronavirus polymerase: implications for biochemical functions and drug design. Nucleic Acids Res 31: 7117\u20137130.\u00a0\u00a0\u00a0 \u300831. Xu X\u3001Liu Y\u3001Weiss S\u3001Arnold E\u3001Sarafianos SG \u7b49\u3002 (2003) SARS \u51a0\u72c0\u75c5\u6bd2\u805a\u5408\u9176\u7684\u5206\u5b50\u6a21\u578b\uff1a\u5c0d\u751f\u5316\u529f\u80fd\u548c\u85e5\u7269\u8a2d\u8a08\u7684\u5f71\u97ff\u3002\u6838\u9178\u7814\u7a76 31\uff1a7117-7130\u3002\u3009View Article\u3000Google Scholar 32.Zhai Y, Sun F, Li X, Pang H, Xu X, et al. (2005) Insights into SARS-CoV transcription and replication from the structure of the nsp7-nsp8 hexadecamer. Nat Struct Mol Biol 12: 980\u2013986.\u00a0\u00a0\u00a0 \u300832. \u7fdf\u6bc5\u3001\u5b6b\u98db\u3001\u674e\u65ed\u3001\u9f90\u83ef\u3001\u5f90\u65ed\u7b49\u3002 (2005) \u5f9e nsp7-nsp8 \u5341\u516d\u805a\u9ad4\u7684\u7d50\u69cb\u6d1e\u5bdf SARS-CoV \u8f49\u9304\u548c\u5fa9\u5236\u3002 Nat Struct Mol Biol 12\uff1a980-986\u3002\u3009View Article\u3000Google Scholar 33.Stockman LJ, Bellamy R, Garner P (2006) SARS: Systematic Review of Treatment Effects. PLoS Med 3: e343.\u00a0\u00a0\u00a0 \u300833. Stockman LJ\u3001Bellamy R\u3001Garner P (2006) SARS\uff1a\u6cbb\u7642\u6548\u679c\u7684\u7cfb\u7d71\u8a55\u50f9\u3002 PLoS Med 3: e343\u3002\u3009View Article\u3000Google Scholar 34.Thompson A, Patel K, Tillman H, McHutchison JG (2009) Directly acting antivirals for the treatment of patients with hepatitis C infection: A clinical development update addressing key future challenges. J Hepatol 50: 184\u2013194.\u00a0\u00a0\u00a0 \u300834. Thompson A\u3001Patel K\u3001Tillman H\u3001McHutchison JG (2009) \u7528\u65bc\u6cbb\u7642\u4e19\u578b\u809d\u708e\u611f\u67d3\u60a3\u8005\u7684\u76f4\u63a5\u6297\u75c5\u6bd2\u85e5\u7269\uff1a\u61c9\u5c0d\u672a\u4f86\u95dc\u9375\u6311\u6230\u7684\u81e8\u5e8a\u958b\u767c\u66f4\u65b0\u3002 J Hepatol 50\uff1a184-194\u3002\u3009View Article\u3000Google Scholar 35.De Clercq E (2004) Antivirals and antiviral strategies. Nat Rev Microbiol 2: 704\u2013720.\u00a0\u00a0\u00a0 \u300835. De Clercq E (2004) \u6297\u75c5\u6bd2\u85e5\u7269\u548c\u6297\u75c5\u6bd2\u7b56\u7565\u3002 Nat Rev Microbiol 2: 704\u2013720\u3002\u3009View Article\u3000Google Scholar 36.Thompson AJV, McHutchison JG (2009) Antiviral resistance and specifically targeted therapy for HCV (STAT-C). J Viral Hepat 16: 377\u2013387.\u00a0\u00a0\u00a0 \u300836. Thompson AJV, McHutchison JG (2009) HCV \u6297\u75c5\u6bd2\u8010\u85e5\u6027\u548c\u7279\u7570\u6027\u9776\u5411\u6cbb\u7642 (STAT-C)\u3002 J \u75c5\u6bd2\u6027\u809d\u75c5 16\uff1a377-387\u3002\u3009View Article\u3000Google Scholar 37.Yang W, Lee JY, Nowotny M (2006) Making and Breaking Nucleic Acids: Two-Mg2+-Ion Catalysis and Substrate Specificity. Mol Cell 22: 5\u201313.\u00a0\u00a0\u00a0 \u300837. Yang W\u3001Lee JY\u3001Nowotny M (2006) \u88fd\u9020\u548c\u7834\u58de\u6838\u9178\uff1a\u96d9 Mg2+ \u96e2\u5b50\u50ac\u5316\u548c\u5e95\u7269\u7279\u7570\u6027\u3002\u6469\u723e\u7d30\u80de 22\uff1a5-13\u3002\u3009View Article\u3000Google Scholar 38.Castro C, Smidansky E, Maksimchuk KR, Arnold JJ, Korneeva VS, et al. (2007) Two proton transfers in the transition state for nucleotidyl transfer catalyzed by RNA- and DNA-dependent RNA and DNA polymerases. Proc Natl Acad Sci USA 104: 4267\u20134272.\u00a0\u00a0\u00a0 \u300838. Castro C\u3001Smidansky E\u3001Maksimchuk KR\u3001Arnold JJ\u3001Korneeva VS \u7b49\u3002 (2007) \u7531 RNA \u548c DNA \u4f9d\u8cf4\u6027 RNA \u548c DNA \u805a\u5408\u9176\u50ac\u5316\u7684\u6838\u82f7\u9178\u8f49\u79fb\u7684\u5169\u500b\u904e\u6e21\u614b\u8cea\u5b50\u8f49\u79fb\u3002 Proc Natl Acad Sci USA 104\uff1a4267\u20134272\u3002\u3009View Article\u3000Google Scholar 39.Arnold JJ, Ghosh SK, Cameron CE (1999) Poliovirus RNA-dependent RNA Polymerase (3Dpol). Divalent cation modulation of primer, template and nucleotide selection. J Biol Chem 274: 37060\u201337069.\u00a0\u00a0\u00a0 \u300839. Arnold JJ\u3001Ghosh SK\u3001Cameron CE (1999) \u810a\u9ad3\u7070\u8cea\u708e\u75c5\u6bd2 RNA \u4f9d\u8cf4\u6027 RNA \u805a\u5408\u9176 (3Dpol)\u3002\u5f15\u7269\u3001\u6a21\u677f\u548c\u6838\u82f7\u9178\u9078\u64c7\u7684\u4e8c\u50f9\u967d\u96e2\u5b50\u8abf\u7bc0\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 274\uff1a37060-37069\u3002\u3009View Article\u3000Google Scholar 40.Iuchi S (2001) Three classes of C2H2 zinc finger proteins. Cell Mol Life Sci 58: 625\u2013635.\u00a0\u00a0\u00a0 \u300840. Iuchi S (2001) \u4e09\u985e C2H2 \u92c5\u6307\u86cb\u767d\u3002\u7d30\u80de\u5206\u5b50\u751f\u547d\u79d1\u5b78 58\uff1a625-635\u3002\u3009View Article\u3000Google Scholar 41.Gomis-Ruth XF (2009) Catalytic domain architecture of metzincin metalloproteases. J Biol Chem 284: 15353\u201315357.\u00a0\u00a0\u00a0 \u300841. Gomis-Ruth XF (2009) Metzincin \u91d1\u5c6c\u86cb\u767d\u9176\u7684\u50ac\u5316\u57df\u7d50\u69cb\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 284\uff1a15353\u201315357\u3002\u3009View Article\u3000Google Scholar 42.Yap TL, Xu T, Chen Y-L, Malet H, Egloff M-P, et al. (2007) Crystal Structure of the Dengue Virus RNA-Dependent RNA Polymerase Catalytic Domain at 1.85-Angstrom Resolution. J Virol 81: 4753\u20134765.\u00a0\u00a0\u00a0 \u300842. Yap TL\u3001Xu T\u3001Chen Y-L\u3001Malet H\u3001Egloff M-P \u7b49\u3002 (2007) \u5206\u8fa8\u7387\u70ba 1.85 \u57c3\u7684\u767b\u9769\u71b1\u75c5\u6bd2\u4f9d\u8cf4\u65bc RNA \u7684 RNA \u805a\u5408\u9176\u50ac\u5316\u7d50\u69cb\u57df\u7684\u6676\u9ad4\u7d50\u69cb\u3002 J Virol 81\uff1a4753\u20134765\u3002\u3009View Article\u3000Google Scholar 43.Winek CL, Buehler EV (1966) Intravenous toxicity of zinc pyridinethione and several zinc salts. Toxicol Appl Pharmacol 9: 296\u2013273.\u00a0\u00a0\u00a0 \u300843. Winek CL, Buehler EV (1966) \u5421\u5576\u786b\u916e\u92c5\u548c\u5e7e\u7a2e\u92c5\u9e7d\u7684\u975c\u8108\u6bd2\u6027\u3002 Toxicol Appl Pharmacol 9\uff1a296-273\u3002\u3009View Article\u3000Google Scholar 44.Snyder DR, de Jesus CP, Towfighi J, Jacoby RO, Wedig JH (1979) Neurological, microscopic and enzyme-histochemical assessment of zinc pyrithione toxicity. Food Cosmet Toxicol 17: 651\u2013660.\u00a0\u00a0\u00a0 \u300844. Snyder DR, de Jesus CP, Towfighi J, Jacoby RO, Wedig JH (1979) \u5421\u5576\u786b\u916e\u92c5\u6bd2\u6027\u7684\u795e\u7d93\u5b78\u3001\u986f\u5fae\u93e1\u548c\u9176\u7d44\u7e54\u5316\u5b78\u8a55\u4f30\u3002\u98df\u54c1\u5316\u599d\u54c1\u6bd2\u7406\u5b78 17\uff1a651-660\u3002\u3009View Article\u3000Google Scholar 45.Magda D, Lecane P, Wang Z, Hu W, Thiemann P, et al. (2008) Synthesis and anticancer properties of water-soluble zinc ionophores. Cancer Res 68: 5318\u20135325.\u00a0\u00a0\u00a0 \u300845. Magda D\u3001Lecane P\u3001Wang Z\u3001Hu W\u3001Thiemann P \u7b49\u3002 (2008) \u6c34\u6eb6\u6027\u92c5\u96e2\u5b50\u8f09\u9ad4\u7684\u5408\u6210\u548c\u6297\u764c\u7279\u6027\u3002\u764c\u75c7\u7814\u7a76 68\uff1a5318-5325\u3002\u3009View Article\u3000oogle Scholar 46.Snijder EJ, van der Meer Y, Zevenhoven-Dobbe J, Onderwater JJM, van der Meulen J, et al. (2006) Ultrastructure and Origin of Membrane Vesicles Associated with the Severe Acute Respiratory Syndrome Coronavirus Replication Complex. J Virol 80: 5927\u20135940.\u00a0\u00a0\u00a0 \u300846. Snijder EJ\u3001van der Meer Y\u3001Zevenhoven-Dobbe J\u3001Onderwater JJM\u3001van der Meulen J \u7b49\u3002 (2006) \u8207\u56b4\u91cd\u6025\u6027\u547c\u5438\u7cfb\u7d71\u7d9c\u5408\u75c7\u51a0\u72c0\u75c5\u6bd2\u8907\u88fd\u8907\u5408\u9ad4\u76f8\u95dc\u7684\u819c\u56ca\u6ce1\u7684\u8d85\u5fae\u7d50\u69cb\u548c\u8d77\u6e90\u3002 J Virol 80\uff1a5927-5940\u3002\u3009View Article\u3000Google Scholar 47.Studier F (2005) Protein production by auto-induction in high density shaking cultures. Protein Expr Purif 41: 207\u2013234.\u00a0\u00a0\u00a0 \u300847. Studier F (2005) \u5728\u9ad8\u5bc6\u5ea6\u632f\u76ea\u57f9\u990a\u4e2d\u901a\u904e\u81ea\u52d5\u8a98\u5c0e\u7522\u751f\u86cb\u767d\u8cea\u3002\u86cb\u767d\u8cea Expr Purif 41\uff1a207-234\u3002\u3009View Article\u3000Google Scholar   \u91cd\u5fb7\u4e26\u4e14\u5c07\u5728\u5317\u4eac\u7684\u300c\u4e2d\u570b\u79d1\u5b78\u9662\u751f\u7269\u7269\u7406\u7814\u7a76\u6240\u611f\u67d3\u8207\u514d\u75ab\u5b78\u91cd\u9ede\u5be6\u9a57\u5ba4\u300d\u5728\u91ab\u5b78\u96dc\u8a8c\u4e0a\u6240\u767c\u8868\u7684\u4e00\u7bc7\u82f1\u6587\u7814\u7a76\u5831\u544a\u300a\u92c5\u6307\u6297\u75c5\u6bd2\u86cb\u767d\u6291\u5236\u7532\u578b\u6d41\u611f\u75c5\u6bd2\u86cb\u767d\u8868\u9054\u4ee5\u53ca\u88ab\u75c5\u6bd2\u7de8\u78bcNS1 \u5c0d\u6297\u7684\u7c21\u8981\u5f62\u5f0f\u300b\u7684\u4e2d\u6587\u81ea\u8b6f\u7248\u4e5f\u5bc4\u4e0a\u7d66\u4ed6\u53c3\u8003(\u5982\u9644\u6a94)\u3002 \u4e26\u4e14\uff0c\u91cd\u5fb7\u9084\u767c\u53bb\u4ee5\u4e0b\u5716\u7247\u8207\u8aaa\u660e\uff1a   \u300e\u9019\u5c31\u662f\u92c5\u6307\u7d50\u69cb\u5716\uff0c\u7531\u65bc\u9019\u662f\u87af\u5408\u7d50\u69cb\uff0c\u56e0\u6b64\u5b83\u80fd\u5f88\u5bb9\u6613\u5c07\u92c5\u96e2\u5b50\u7a7f\u900f\u7d30\u80de\u58c1\u800c\u8207\u8eb2\u85cf\u5728\u7d30\u80de\u5167\u840c\u767c\u7684\u75c5\u6bd2\u56e0\u70ba\u767c\u751f\u6291\u5236\u7ffb\u8b6f\u548c\u4fc3\u9032\u75c5\u6bd2 mRNA\u7684\u964d\u89e3\uff0c\u56e0\u6b64\u901a\u904e PARP \u7d50\u69cb\u57df\u800c\u4fc3\u9032 IAV \u86cb\u767d\u964d\u89e3\uff0c\u5be6\u9a57\u5df2\u7d93\u8b49\u5be6\u5b83\u53ef\u8b93\u5165\u4fb5\u5230\u7d30\u80de\u5167\u7684\u75c5\u6bd2\u4e4bRNA\u6a5f\u8f49\u767c\u751f\u4f11\u514b(\u4f46\u4e0d\u4e00\u5b9a\u6703\u6b7b\u4ea1)\uff0c\u800c\u4f7f\u5f97\u539f\u672c\u7121\u751f\u547d\u7684\u75c5\u6bd2\u56e0\u6b64\u4e0d\u80fd\u5927\u91cf\u8907\u88fd\u840c\u751f\uff0c\u800c\u53ef\u4ee5\u70ba\u4eba\u9ad4\u7684\u514d\u75ab\u7cfb\u7d71\u89e3\u6c7a\u3002\u300f \u9019\u4e00\u4f4d\u738b\u59d3\u7db2\u53cb\u53c8\u767c\u4f86\u4ee5\u4e0b\u4fe1\u606f\uff1a\u300e\u80fd\u5920\u6bba\u75c5\u6bd2\u800c\u4e0d\u6703\u6bba\u6b7b\u6211\u5011\u81ea\u5df1\u7684\u7d30\u80de,\u5929\u4e0b\u6709\u9019\u9ebc\u597d\u7684\u4e8b\u55ce\uff1f\u4e0d\u904e\u662f\u71df\u990a\u88dc\u52a9\u54c1\u800c\u5df2\uff01\u300f \u91cd\u5fb7\u770b\u4ed6\u5728\u610f\u6c23\u7528\u4e8b\uff0c\u56e0\u6b64\u5c31\u56de\u8986\u4ed6\u8aaa\uff1a\u300e\u96a8\u60a8\u9ad8\u8208\u600e\u9ebc\u8aaa\u90fd\u597d\uff0c\u6211\u6c92\u6709\u8aaa\u5b83\u662f\u85e5\uff0c\u53ea\u8aaa\u662f\u4fdd\u5065\u54c1\u800c\u5df2\u3002\u300f\u767c\u5b8c\u4e86\u9019\u4fe1\u606f\u4e4b\u5f8c\uff0c\u91cd\u5fb7\u767c\u8a93\u4ee5\u5f8c\u518d\u4e5f\u4e0d\u5c0d\u9019\u7a2e\u96de\u86cb\u88e1\u6311\u9aa8\u982d\u7684\u4eba\u63d0\u4f9b\u4efb\u4f55\u6709\u50f9\u503c\u7684\u8cc7\u8a0a\u4e86\u3002 \u4f46\u662f\uff0c\u91cd\u5fb7\u9084\u662f\u8981\u544a\u8a34\u5927\u5bb6\u70ba\u4f55\u8981\u5efa\u8b70\u5927\u5bb6\u5403\u542b\u92c5\u96e2\u5b50\u7684\u4fdd\u5065\u98df\u54c1\uff0c\u7576\u7136\u92c5\u5f9e\u98df\u7269\u4e2d\u651d\u53d6\u4e5f\u6709\uff0c\u4f46\u662f\u98df\u7269\u5fc5\u9808\u7d93\u904e\u6d88\u5316\u5f8c\u92c5\u96e2\u5b50\u624d\u6703\u900f\u904e\u8840\u6db2\u800c\u5230\u4eba\u9ad4\u5668\u5b98\uff0c\u9019\u500b\u904e\u7a0b\u592a\u9577\u592a\u4e45\u4e86\uff0c\u56e0\u6b64\u92c5\u96e2\u5b50\u53ef\u80fd\u53c8\u8207\u5176\u4ed6\u7269\u8cea\u7d50\u5408\u6210\u5316\u5408\u7269\uff0c\u800c\u518d\u4e0d\u80fd\u767c\u751f\u8207\u8ca0\u96e2\u5b50\u7684\u75c5\u6bd2\u4e2d\u548c\u7684\u6548\u679c\u3002\u56e0\u6b64\u5c31\u66f4\u6c92\u6709\u767c\u63ee\u5230\u92c5\u96e2\u5b50\u6240\u7368\u5177\u7684\u87af\u5408\u4ea4\u63db\u6548\u679c\u3002 \u7531\u65bc\uff0c\u611f\u5192\u6216\u662fCOVID-19\u7684\u521d\u671f\u75c5\u72c0\u4e3b\u8981\u90fd\u662f\u91dd\u5c0d\u4e0a\u652f\u6c23\u7ba1\uff0c\u56e0\u6b64\u4ee3\u8868\u547c\u5438\u7ba1\u9053\u7684\u75c5\u6bd2\u91cf\u6700\u5927\uff0c\u56e0\u6b64\u53ef\u4ee5\u542b\u5728\u5634\u4e2d\u6162\u6162\u5206\u89e3\u7684\u5589\u7cd6\u4e4b\u6548\u679c\u6700\u597d\uff0c\u56e0\u70ba\u5b83\u8b93\u901a\u904e\u7684\u6c23\u6d41\u4e2d\u7684\u75c5\u6bd2\u5148\u88ab\u92c5\u96e2\u5b50\u4e2d\u548c\uff0c\u800c\u7cd6\u6c41\u6d41\u5165\u98df\u9053\u6642\u92c5\u96e2\u5b50\u4e5f\u6703\u6ea2\u5165\u4e0a\u652f\u6c23\u7ba1\u3002\u9019\u5c31\u662f\u70ba\u4f55\u91cd\u5fb7\u9996\u5148\u63a8\u85a6\u5df2\u7d93\u5728\u7f8e\u570b\u98a8\u884c30\u591a\u5e74\u7684\u611f\u5192\u9810\u9632\u7528\u85e5Cold Eeze\u4e2d\u6587\u8b6f\u540d\u70ba\u3010\u92c5\u7cd6\u3011\uff0c\u56e0\u70ba\u5b83\u5df2\u7d93\u904e\u4e86\u5c08\u5229\u4fdd\u8b77\u671f\uff0c\u5b78\u540d\u85e5\u540d\u662fGeneric Version\uff0c\u56e0\u70ba\u552e\u50f9\u8f03\u4fbf\u5b9c(\u4f46\u662f\u73fe\u5728\u5168\u7403\u6436\u8cfc\u5f88\u53ef\u80fd\u7f3a\u8ca8)\u3002 \u73fe\u5728\uff0c\u91cd\u5fb7\u8981\u544a\u8a34\u5927\u5bb6\u4e00\u500b\u79d8\u5bc6\uff0c\u90a3\u5c31\u662f\u70ba\u4f55\u91cd\u5fb7\u8981\u5c0d\u92c5\u96e2\u5b50\u9032\u884c\u7814\u7a76\u5462\uff0c\u6545\u4e8b\u662f\u9019\u6a23\u5b50\u7684\uff0c\u7531\u65bc\u4e00\u5e74\u524d \u91cd\u5fb7 \u8acb\u793a\u201c\u9748\u7236\u201d(\u5143\u59cb\u5929\u5c0a)\u8aaa\uff1a\u300e\u65e2\u7136\u00a0 \u9748\u7236\u80fd\u63d0\u524d\u534a\u5e74\u8b66\u544a\u6211\u5011\u5c07\u6709\u6b64\u5927\u75ab\u60c5\uff0c\u662f\u5426\u80fd\u544a\u77e5\u5f92\u5152\u5982\u4f55\u88fd\u5f97\u89e3\u85e5\uff1f\u300f\u64f2\u832d\u61c9\u8a31\u5f8c\uff0c\u91cd\u5fb7 \u6b63\u4e0d\u77e5\u5982\u4f55\u518d\u554f\u9019\u89e3\u85e5\u79d8\u65b9\u6642\uff0c\u8166\u4e2d\u7a81\u7136\u6e67\u73fe\u4e00\u500b\u201c\u92c5\u201d\u5b57\uff0c\u64f2\u832d\u518d\u554f\u78ba\u5b9a\u795e\u8aed\u5c31\u662f\u6b64\u201c\u92c5\u201d\u5b57\u7121\u8aa4\u3002 \u5728\u6b64\u4e4b\u5f8c\uff0c\u91cd\u5fb7\u624d\u958b\u59cb\u8a73\u95b1\u5404\u7a2e\u82f1\u6587\u91ab\u5b78\u6587\u737b\uff0c\u8d8a\u767c\u8b49\u5be6\u92c5\u96e2\u5b50\u6709\u6291\u5236mRNA\u75c5\u6bd2\u7684\u6548\u679c\uff0c\u800c\u4e14\u56e0\u70ba\u92c5\u96e2\u5b50\u662f\u63a1\u53d6\u87af\u5408\u65b9\u5f0f\u8b93\u92c5\u96e2\u5b50\u7a7f\u900f\u7d30\u80de\u58c1\u9032\u5165\u4f86\u964d\u89e3\u75c5\u6bd2\u86cb\u767d\u8907\u88fd\u6240\u9700\u6c42\u7684PARP \u7d50\u69cb\u57df\uff0c\u56e0\u6b64\u9019\u662f\u4e00\u7a2e\u7cbe\u6e96\u7834\u7a97\u5f0f\u7684\u65ac\u9996\u884c\u52d5\uff0c\u5c0d\u65bc\u7d30\u80de\u7684\u50b7\u5bb3\u751a\u5c0f\uff0c\u56e0\u6b64\u5c0d\u65bc\u4eba\u9ad4\u7684\u5371\u5bb3\u751a\u4f4e\u3002 \u91cd\u5fb7\u4e00\u5e74\u524d\u5c31\u628a\u7814\u7a76\u6210\u679c\u516c\u958b\uff0c\u4e00\u4f86\u56e0\u70ba\u81ea\u5df1\u962e\u56ca\u7f9e\u6f80\uff0c\u7121\u6cd5\u7e7c\u7e8c\u7814\u767c\uff0c\u4e8c\u4f86\u91cd\u5fb7\u5df2\u7d93\u9810\u7aba\u5230\u672a\u4f86\u9084\u6709\u5176\u4ed6\u56b4\u91cd\u75be\u75c5\uff0c\u800c\u4e14COVID-19\u9084\u6703\u5927\u7a81\u8b8a\uff0c\u56e0\u6b64\u8b93\u5176\u4ed6\u4eba\u63a5\u68d2\u4f86\u7814\u7a76\u6216\u8a31\u6703\u66f4\u6709\u6210\u6548\u3002","og_url":"https:\/\/pingtungtimes.com.tw\/?p=104514","og_site_name":"\u5c4f\u6771\u6642\u5831\u96fb\u5b50\u5831","article_publisher":"https:\/\/www.facebook.com\/pingtungnews","article_published_time":"2022-05-27T15:08:21+00:00","og_image":[{"width":897,"height":468,"url":"https:\/\/pingtungtimes.com.tw\/wp-content\/uploads\/2022\/05\/\u4e00\u822c\u4eba\u5f9e\u98df\u7269\u4e2d\u651d\u53d6\u92c5\u70ba\u7684\u5c31\u662f\u58ef\u967d\u8207\u589e\u9aee.jpg","type":"image\/jpeg"}],"author":"fanjiang","twitter_card":"summary_large_image","twitter_misc":{"\u4f5c\u8005:":"fanjiang","\u9810\u4f30\u95b1\u8b80\u6642\u9593":"9 \u5206\u9418"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"NewsArticle","@id":"https:\/\/pingtungtimes.com.tw\/?p=104514#article","isPartOf":{"@id":"https:\/\/pingtungtimes.com.tw\/?p=104514"},"author":{"name":"fanjiang","@id":"https:\/\/pingtungtimes.com.tw\/#\/schema\/person\/90cf880d4d210d8db447ae0199435aa4"},"headline":"\u597d\u201c\u92c5\u201d\u3000\u4e5f\u6703\u60f9\u4e86\u4e00\u8eab\u8165","datePublished":"2022-05-27T15:08:21+00:00","mainEntityOfPage":{"@id":"https:\/\/pingtungtimes.com.tw\/?p=104514"},"wordCount":1787,"commentCount":0,"publisher":{"@id":"https:\/\/pingtungtimes.com.tw\/#organization"},"image":{"@id":"https:\/\/pingtungtimes.com.tw\/?p=104514#primaryimage"},"thumbnailUrl":"https:\/\/pingtungtimes.com.tw\/wp-content\/uploads\/2022\/05\/\u4e00\u822c\u4eba\u5f9e\u98df\u7269\u4e2d\u651d\u53d6\u92c5\u70ba\u7684\u5c31\u662f\u58ef\u967d\u8207\u589e\u9aee.jpg","keywords":["\u674e\u91cd\u5fb7","\u78ba\u8a3a\u7652\u5f8c\u8005","\u88dc\u5145\u92c5\u96e2\u5b50\u98df\u54c1"],"articleSection":["\u9577\u7167\u5c08\u6b04"],"inLanguage":"zh-TW","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/pingtungtimes.com.tw\/?p=104514#respond"]}]},{"@type":"WebPage","@id":"https:\/\/pingtungtimes.com.tw\/?p=104514","url":"https:\/\/pingtungtimes.com.tw\/?p=104514","name":"\u597d\u201c\u92c5\u201d\u3000\u4e5f\u6703\u60f9\u4e86\u4e00\u8eab\u8165 - \u5c4f\u6771\u6642\u5831\u96fb\u5b50\u5831","isPartOf":{"@id":"https:\/\/pingtungtimes.com.tw\/#website"},"primaryImageOfPage":{"@id":"https:\/\/pingtungtimes.com.tw\/?p=104514#primaryimage"},"image":{"@id":"https:\/\/pingtungtimes.com.tw\/?p=104514#primaryimage"},"thumbnailUrl":"https:\/\/pingtungtimes.com.tw\/wp-content\/uploads\/2022\/05\/\u4e00\u822c\u4eba\u5f9e\u98df\u7269\u4e2d\u651d\u53d6\u92c5\u70ba\u7684\u5c31\u662f\u58ef\u967d\u8207\u589e\u9aee.jpg","datePublished":"2022-05-27T15:08:21+00:00","description":"\u3010\u674e\u91cd\u5fb7\uff0f\u5c08\u6b04\u5831\u5c0e2022\/05\/27\u3011\u3000\u4eca\u65e9\uff0c\u6709\u4e00\u78ba\u8a3a\u7652\u5f8c\u8005\u8207\u91cd\u5fb7\u8a0e\u8ad6\u4ed6\u7684\u75c5\u60c5\uff0c\u91cd\u5fb7\u5efa\u8b70\u4ed6\u88dc\u5145\u92c5\u96e2\u5b50\u98df\u54c1\uff0c\u4e26\u5c07\u6b64\u4e00\u4fe1\u606f\u4e5f\u767c\u7d66\u5927\u5bb6\u77e5\u66c9\uff1a \u300e\u6709\u78ba\u8a3a\u7652\u5f8c\u8005\u554f\u91cd\u5fb7\u8981\u5982\u4f55\u4fdd\u5065\u4ee5\u9632\u5fa9\u967d\uff0c\u91cd\u5fb7 \u7686\u5efa\u8b70\u4ed6\u5403\u3010\u92c5\u7cd6\u3011(\u985e\u4f3c\u5589\u7247)\u5c0f\u5b69\u5247\u5403\u3010\u5152\u7ae5\u92c5\u8edf\u7cd6\u3011\uff0c\u6216\u662f\u3010\u9175\u6bcd\u92c5\u3011\u3001\u3010\u87af\u5408\u92c5\u3011\u6216\u662f\u3010\u8461\u8404\u7cd6\u9178\u92c5\u3011\u4f86\u6d88\u6ec5\u6f5b\u85cf\u4eba\u9ad4\u5c1a\u672a\u767c\u4f5c\u7684\u75c5\u6bd2\u3002\u300f \u5f8c\u4f86\u5c31\u63a5\u7372\u4e00\u4f4d\u738b\u59d3\u7db2\u53cb\u56de\u8986\u8aaa\uff1a\u300e\u4e0d\u5c0d\u5594\uff01\u92c5,\u4eba\u985e\u5b58\u6d3b\u6240\u9700\u8981\u7684\u5143\u7d20\u4e4b\u4e00,\u5f9e\u5929\u7136\u98df\u7269\u76f4\u63a5\u651d\u53d6\u5c31\u8db3\u5920\u4e86,\u4eba\u70ba\u8d85\u91cf\u7684\u88dc\u5145\u6703\u51fa\u554f\u984c\u7684\uff01\u92c5,\u4e0d\u6703\u76f4\u63a5\u6bba\u75c5\u6bd2\u7684\uff01\u300f \u9019\u4e00\u4f4d\u738b\u5148\u751f\uff0c\u5927\u6982\u6f0f\u770b\u4e86\u91cd\u5fb7\u5728\u4e00\u5e74\u524d\u5728\u672c\u6703\u7fa4\u7d44\u4e0a\u767c\u8868\u7684\u5e7e\u7bc7\u4ee5\u92c5\u96e2\u5b50\u4f86\u6d88\u6ec5\u75c5\u6bd2\u7684\u6587\u7ae0\uff0c\u56e0\u6b64\uff0c\u91cd\u5fb7\u5c31\u56de\u8986\u4ed6\u8aaa\uff1a\u300e\u6211\u770b\u904e\u4e86\u8a31\u591a\u82f1\u6587\u91ab\u5b78\u6587\u737b\uff0c\u4e5f\u8207\u9ec3\u25cb\u5bcc\u535a\u58eb\u548c\u9673\u25cb\u5e73\u91ab\u5e2b\u8a0e\u8ad6\u904e\u3002\u60a8\u5982\u679c\u60f3\u8981\u770b\u9019\u4e00\u4e9b\u82f1\u6587\u91ab\u5b78\u6587\u737b\uff0c\u6211\u53ef\u4ee5\u5bc4\u53bb\u7d66\u60a8\u3002\u300f\u7136\u5f8c \u91cd\u5fb7 \u5c31\u5c31\u5bc4\u53bb\u7d66\u4ed6\u4ee5\u4e0b\u7684\u8cc7\u6599\uff1a References\u53c3\u8003\u6587\u737b 1.Lazarczyk M, Favre M (2008) Role of Zn2+ ions in host-virus interactions. J Virol 82: 11486\u201311494.\u00a0\u00a0\u00a0 \u30081. Lazarczyk M, Favre M (2008) Zn2+ \u96e2\u5b50\u5728\u5bbf\u4e3b-\u75c5\u6bd2\u76f8\u4e92\u4f5c\u7528\u4e2d\u7684\u4f5c\u7528\u3002 J Virol 82\uff1a11486\u201311494\u3002\u3009\u3000View Article\u3000Google Scholar 2.Frederickson CJ, Koh JY, Bush AI (2005) Neurobiology of zinc in health and disease. Nat Rev Neurosci 6: 449\u2013462.\u00a0\u00a0\u00a0 \u30082. Frederickson CJ\u3001Koh JY\u3001Bush AI (2005) \u92c5\u5728\u5065\u5eb7\u548c\u75be\u75c5\u4e2d\u7684\u795e\u7d93\u751f\u7269\u5b78\u3002 Nat Rev Neurosci 6\uff1a449-462\u3002\u3009View Article\u3000Google Scholar 3.Alirezaei M, Nairn AC, Glowinski J, Premont J, Marin P (1999) Zinc inhibits protein synthesis in neurons: potential rol of phosphorylation of translation initiation factor-2a. J Biol Chem 274: 32433\u201332438.\u00a0\u00a0\u00a0 \u30083. Alirezaei M\u3001Nairn AC\u3001Glowinski J\u3001Premont J\u3001Marin P (1999) \u92c5\u6291\u5236\u795e\u7d93\u5143\u4e2d\u7684\u86cb\u767d\u8cea\u5408\u6210\uff1a\u7ffb\u8b6f\u8d77\u59cb\u56e0\u5b50 2a \u78f7\u9178\u5316\u7684\u6f5b\u5728\u4f5c\u7528\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 274\uff1a32433\u201332438\u3002\u3009View Article\u3000Google Scholar 4.Uchide N, Ohyama K, Bessho T, Yuan B, Yamakawa T (2002) Effect of antioxidants on apoptosis induced by influenza virus infection: inhibition of viral gene replication and transcription with pyrrolidine dithiocarbamate. Antiviral Res 56: 207\u2013217.\u00a0\u00a0\u00a0 \u30084. Uchide N, Ohyama K, Bessho T, Yuan B, Yamakawa T (2002) \u6297\u6c27\u5316\u5291\u5c0d\u6d41\u611f\u75c5\u6bd2\u611f\u67d3\u8a98\u5c0e\u7684\u7d30\u80de\u51cb\u4ea1\u7684\u5f71\u97ff\uff1a\u7528\u5421\u54af\u70f7\u4e8c\u786b\u4ee3\u6c28\u57fa\u7532\u9178\u916f\u6291\u5236\u75c5\u6bd2\u57fa\u56e0\u8907\u5236\u548c\u8f49\u9304\u3002\u6297\u75c5\u6bd2\u7814\u7a76 56\uff1a207-217\u3002\u3009View Article\u3000Google Scholar 5.Suara RO, Crowe JEJ (2004) Effect of zinc salts on respiratory syncytial virus replication. Antimicrob Agents Chemother 48: 783\u2013790.\u00a0\u00a0\u00a0 \u30085. Suara RO, Crowe JEJ (2004) \u92c5\u9e7d\u5c0d\u547c\u5438\u9053\u5408\u80de\u75c5\u6bd2\u8907\u88fd\u7684\u5f71\u97ff\u3002\u6297\u5fae\u751f\u7269\u5291 Chemother 48\uff1a783-790\u3002\u3009View Article\u3000Google Scholar 6.Gaudernak E, Seipelt J, Triendl A, Grassauer A, Kuechler E (2002) Antiviral Effects of Pyrrolidine Dithiocarbamate on Human Rhinoviruses. J Virol 76: 6004\u20136015.\u00a0\u00a0\u00a0 \u30086. Gaudernak E\u3001Seipelt J\u3001Triendl A\u3001Grassauer A\u3001Kuechler E (2002) \u5421\u54af\u70f7\u4e8c\u786b\u4ee3\u6c28\u57fa\u7532\u9178\u916f\u5c0d\u4eba\u9f3b\u75c5\u6bd2\u7684\u6297\u75c5\u6bd2\u4f5c\u7528\u3002 J Virol 76\uff1a6004-6015\u3002\u3009View Article\u3000Google Scholar 7.Si X, McManus BM, Zhang J, Yuan J, Cheung C, et al. (2005) Pyrrolidine Dithiocarbamate Reduces Coxsackievirus B3 Replication through Inhibition of the Ubiquitin-Proteasome Pathway. J Virol 79: 8014\u20138023.\u00a0\u00a0\u00a0 \u30087. Si X, McManus BM, Zhang J, Yuan J, Cheung C, et al. (2005) Pyrrolidine Dithiocarbamate \u901a\u904e\u6291\u5236\u6cdb\u7d20-\u86cb\u767d\u9176\u9ad4\u9014\u5f91\u6e1b\u5c11\u67ef\u85a9\u5947\u75c5\u6bd2 B3 \u8907\u88fd\u3002 J Virol 79\uff1a8014-8023\u3002\u3009View Article\u3000Google Scholar 8.Korant BD, Kauer JC, Butterworth BE (1974) Zinc ions inhibit replication of rhinoviruses. Nature 248: 588\u2013590.\u00a0\u00a0\u00a0 \u30088. Korant BD, Kauer JC, Butterworth BE (1974) \u92c5\u96e2\u5b50\u6291\u5236\u9f3b\u75c5\u6bd2\u7684\u8907\u5236\u3002\u81ea\u7136 248\uff1a588-590\u3002\u3009View Article\u3000Google Scholar 9.Polatnick J, Bachrach HL (1978) Effect of zinc and other chemical agents on foot-and-mouth-disease virus replication. Antimicrob Agents Chemother 13: 731\u2013734.\u00a0\u00a0 \u00a0\u30089. Polatnick J, Bachrach HL (1978) \u92c5\u548c\u5176\u4ed6\u5316\u5b78\u8a66\u5291\u5c0d\u53e3\u8e44\u75ab\u75c5\u6bd2\u8907\u88fd\u7684\u5f71\u97ff\u3002\u6297\u5fae\u751f\u7269\u5291 Chemother 13\uff1a731-734\u3002\u3009View Article\u3000Google Scholar 10.Lanke K, Krenn BM, Melchers WJG, Seipelt J, van Kuppeveld FJM (2007) PDTC inhibits picornavirus polyprotein processing and RNA replication by transporting zinc ions into cells. J Gen Virol 88: 1206\u20131217.\u00a0\u00a0\u00a0 \u300810. Lanke K\u3001Krenn BM\u3001Melchers WJG\u3001Seipelt J\u3001van Kuppeveld FJM (2007) PDTC \u901a\u904e\u5c07\u92c5\u96e2\u5b50\u8f49\u904b\u5230\u7d30\u80de\u4e2d\u4f86\u6291\u5236\u5c0f\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u591a\u86cb\u767d\u52a0\u5de5\u548c RNA \u8907\u88fd\u3002 J Gen Virol 88\uff1a1206-1217\u3002\u3009View Article\u3000Google Scholar7 11.Krenn BM, Gaudernak E, Holzer B, Lanke K, Van Kuppeveld FJM, et al. (2009) Antiviral Activity of the Zinc Ionophores Pyrithione and Hinokitiol against Picornavirus Infections. J Virol 83: 58\u201364.\u00a0\u00a0\u00a0 \u300811. Krenn BM\u3001Gaudernak E\u3001Holzer B\u3001Lanke K\u3001Van Kuppeveld FJM \u7b49\u3002 (2009) \u92c5\u96e2\u5b50\u8f09\u9ad4\u5421\u5576\u786b\u916e\u548c\u6a9c\u9187\u5c0d\u5c0f\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u611f\u67d3\u7684\u6297\u75c5\u6bd2\u6d3b\u6027\u3002 J Virol 83\uff1a58-64\u3002\u3009View Article\u3000Google Scholar 12.Zalewski PD, Forbes IJ, Betts WH (1993) Correlation of apoptosis with change in intracellular labile Zn(II) using Zinquin , a new specific fluorescent probe for Zn(II). Biochem J 296: 403\u2013408.\u00a0 \u300812. Zalewski PD, Forbes IJ, Betts WH (1993) \u4f7f\u7528 Zinquin \uff0c\u7d30\u80de\u51cb\u4ea1\u8207\u7d30\u80de\u5167\u4e0d\u7a69\u5b9a\u92c5\uff08II\uff09\u8b8a\u5316\u7684\u76f8\u95dc\u6027\uff0c\u4e00\u7a2e\u65b0\u7684\u7279\u7570\u6027Zn(II) \u7684\u7192\u5149\u63a2\u91dd\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 296\uff1a403-408\u3002\u3009View Article\u3000Google Scholar 13.Baum EZ, Bebernitz GA, Palant O, Mueller T, Plotch SJ (1991) Purification, properties, and mutagenesis of poliovirus 3C protease. Virology 165: 140\u2013150.\u00a0\u00a0\u00a0 \u300813. Baum EZ\u3001Bebernitz GA\u3001Palant O\u3001Mueller T\u3001Plotch SJ (1991) \u810a\u9ad3\u7070\u8cea\u708e\u75c5\u6bd2 3C \u86cb\u767d\u9176\u7684\u7d14\u5316\u3001\u7279\u6027\u548c\u8a98\u8b8a\u3002\u75c5\u6bd2\u5b78 165\uff1a140-150\u3002\u3009View Article\u3000Google Scholar 14.Cordingley MG, Register RB, Callahan PL, Garsky VM, Colonno RJ (1989) Cleavage of small peptides in vitro by human rhinovirus 14 3C protease expressed in Escherichia coli. J Virol 63: 5037\u20135045.\u00a0\u00a0\u00a0 \u300814. Cordingley MG\u3001Register RB\u3001Callahan PL\u3001Garsky VM\u3001Colonno RJ (1989) \u5728\u5927\u8178\u687f\u83cc\u4e2d\u8868\u9054\u7684\u4eba\u9f3b\u75c5\u6bd2 14 3C \u86cb\u767d\u9176\u9ad4\u5916\u88c2\u89e3\u5c0f\u80bd\u3002 J Virol 63\uff1a5037-5045\u3002\u3009View Article\u3000Google Scholar 15.Ferrari E, Wright-Minogue J, Fang JW, Baroudy BM, Lau JY, et al. (1999) Characterization of soluble hepatitis C virus RNA-dependent RNA polymerase expressed in Escherichia coli. J Virol 73: 1649\u20131654.\u00a0\u00a0\u00a0 \u300815. Ferrari E\u3001Wright-Minogue J\u3001Fang JW\u3001Baroudy BM\u3001Lau JY \u7b49\u3002 (1999) \u5728\u5927\u8178\u687f\u83cc\u4e2d\u8868\u9054\u7684\u53ef\u6eb6\u6027\u4e19\u578b\u809d\u708e\u75c5\u6bd2 RNA \u4f9d\u8cf4\u6027 RNA \u805a\u5408\u9176\u7684\u8868\u5fb5\u3002 J Virol 73\uff1a1649-1654\u3002\u3009View Article\u3000Google Scholar 16.Hung M, Gibbs CS, Tsiang M (2002) Biochemical characterization of rhinovirus RNA-dependent RNA polymerase. Antiviral Res 56: 99\u2013114.\u00a0\u00a0\u00a0 \u300816. Hung M\u3001Gibbs CS\u3001Tsiang M (2002) \u9f3b\u75c5\u6bd2 RNA \u4f9d\u8cf4\u6027 RNA \u805a\u5408\u9176\u7684\u751f\u5316\u7279\u5fb5\u3002\u6297\u75c5\u6bd2\u7814\u7a76 56\uff1a99-114\u3002\u3009View Article\u3000Google Scholar 17.Perlman S, Netland J (2009) Coronaviruses post-SARS: update on replication and pathogenesis. Nat Rev Micro 7: 439\u2013450.\u00a0\u00a0\u00a0 \u300817. Perlman S, Netland J (2009) SARS \u5f8c\u7684\u51a0\u72c0\u75c5\u6bd2\uff1a\u8907\u88fd\u548c\u767c\u75c5\u6a5f\u5236\u7684\u66f4\u65b0\u3002 Nat Rev Micro 7\uff1a439-450\u3002\u3009View Article\u3000Google Scholar 18.Gorbalenya AE, Enjuanes L, Ziebuhr J, Snijder EJ (2006) Nidovirales: evolving the largest RNA virus genome. Virus Res 117: 17\u201337.\u00a0\u00a0\u00a0 \u300818. Gorbalenya AE\u3001Enjuanes L\u3001Ziebuhr J\u3001Snijder EJ (2006) Nidovirales\uff1a\u9032\u5316\u6700\u5927\u7684 RNA \u75c5\u6bd2\u57fa\u56e0\u7d44\u3002\u75c5\u6bd2\u7814\u7a76 117\uff1a17-37\u3002\u3009View Article\u3000Google Scholar 19.Snijder EJ, Bredenbeek PJ, Dobbe JC, Thiel V, Ziebuhr J, et al. (2003) Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage. J Mol Biol 331: 991\u20131004.\u00a0\u00a0\u00a0 \u300819. Snijder EJ\u3001Bredenbeek PJ\u3001Dobbe JC\u3001Thiel V\u3001Ziebuhr J \u7b49\u3002 (2003) SARS \u51a0\u72c0\u75c5\u6bd2\u57fa\u56e0\u7d44\u548c\u86cb\u767d\u8cea\u7d44\u7684\u7368\u7279\u548c\u4fdd\u5b88\u7279\u5fb5\uff0c\u5f9e\u51a0\u72c0\u75c5\u6bd2\u7d44 2 \u8b5c\u7cfb\u7684\u65e9\u671f\u5206\u88c2\u3002 J Mol Biol 331\uff1a991-1004\u3002\u3009View Article\u3000Google Scholar 20.Pasternak AO, Spaan WJ, Snijder EJ (2006) Nidovirus transcription: how to make sense\u2026? J Gen Virol 80: 1403\u20131421.\u00a0\u00a0\u00a0 \u300820. Pasternak AO\u3001Spaan WJ\u3001Snijder EJ (2006) Nidovirus \u8f49\u9304\uff1a\u5982\u4f55\u7406\u89e3\u2026\u2026\uff1f J Gen Virol 80\uff1a1403\u20131421\u3002\u3009View Article\u3000Google Scholar 21.Sawicki SG, Sawicki DL, Siddell SG (2007) A Contemporary View of Coronavirus Transcription. J Virol 81: 20\u201329.\u00a0\u00a0\u00a0 \u300821. Sawicki SG\u3001Sawicki DL\u3001Siddell SG\uff082007\uff09\u51a0\u72c0\u75c5\u6bd2\u8f49\u9304\u7684\u7576\u4ee3\u89c0\u9ede\u3002 J Virol 81\uff1a20-29\u3002\u3009View Article\u3000Google Scholar 22.Butterworth BE, Korant BD (1974) Characterization of the large picornaviral polypeptides produced in the presence of zinc ion. J Virol 14: 282\u2013291.\u00a0\u00a0\u00a0 \u300822. Butterworth BE, Korant BD (1974) \u5728\u92c5\u96e2\u5b50\u5b58\u5728\u4e0b\u7522\u751f\u7684\u5927\u6838\u7cd6\u6838\u9178\u75c5\u6bd2\u591a\u80bd\u7684\u8868\u5fb5\u3002 J Virol 14\uff1a282-291\u3002\u3009View Article\u3000Google Scholar 23.Denison MR, Perlman S (1986) Translation and processing of mouse hepatitis virus virion RNA in a cell-free system. J Virol 60: 12\u201318.\u00a0\u00a0\u00a0 \u300823. Denison MR, Perlman S (1986) \u5728\u7121\u7d30\u80de\u7cfb\u7d71\u4e2d\u7ffb\u8b6f\u548c\u8655\u7406\u5c0f\u9f20\u809d\u708e\u75c5\u6bd2\u75c5\u6bd2\u9ad4 RNA\u3002 J Virol 60\uff1a12-18\u3002\u3009View Article\u3000Google Scholar 24.Denison MR, Zoltick PW, Hughes SA, Giangreco B, Olson AL, et al. (1992) Intracellular processing of the N-terminal ORF 1a proteins of the coronavirus MHV-A59 requires multiple proteolytic events. Virology 189: 274\u2013284.\u00a0\u00a0\u00a0 \u300824. Denison MR\u3001Zoltick PW\u3001Hughes SA\u3001Giangreco B\u3001Olson AL \u7b49\u3002 (1992) \u51a0\u72c0\u75c5\u6bd2 MHV-A59 \u7684 N \u7aef ORF 1a \u86cb\u767d\u7684\u7d30\u80de\u5167\u52a0\u5de5\u9700\u8981\u591a\u500b\u86cb\u767d\u6c34\u89e3\u4e8b\u4ef6\u3002\u75c5\u6bd2\u5b78 189\uff1a274-284\u3002\u3009View Article\u3000Google Scholar 25.van Hemert MJ, de Wilde AH, Gorbalenya AE, Snijder EJ (2008) The in Vitro RNA Synthesizing Activity of the Isolated Arterivirus Replication\/Transcription Complex Is Dependent on a Host Factor. J Biol Chem 283: 16525\u201316536.\u00a0\u00a0\u00a0 \u300825. van Hemert MJ\u3001de Wilde AH\u3001Gorbalenya AE\u3001Snijder EJ (2008) \u5206\u96e2\u7684\u52d5\u8108\u75c5\u6bd2\u8907\u88fd\/\u8f49\u9304\u8907\u5408\u7269\u7684\u9ad4\u5916 RNA \u5408\u6210\u6d3b\u6027\u53d6\u6c7a\u65bc\u5bbf\u4e3b\u56e0\u5b50\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 283\uff1a16525-16536\u3002\u3009View Article\u3000Google Scholar 26.van Hemert MJ, van den Worm SHE, Knoops K, Mommaas AM, Gorbalenya AE, et al. (2008) SARS-Coronavirus Replication\/Transcription Complexes Are Membrane-Protected and Need a Host Factor for Activity In Vitro. PLoS Pathog 4: e1000054.\u00a0\u00a0\u00a0 \u300826. van Hemert MJ\u3001van den Worm SHE\u3001Knoops K\u3001Mommaas AM\u3001Gorbalenya AE \u7b49\u3002 (2008) SARS-\u51a0\u72c0\u75c5\u6bd2\u8907\u88fd\/\u8f49\u9304\u8907\u5408\u7269\u53d7\u819c\u4fdd\u8b77\uff0c\u9700\u8981\u5bbf\u4e3b\u56e0\u5b50\u624d\u80fd\u9032\u884c\u9ad4\u5916\u6d3b\u52d5\u3002 PLoS \u75c5\u539f\u9ad4 4\uff1ae1000054\u3002\u3009View Article\u3000Google Scholar 27.te Velthuis AJ, Arnold JJ, Cameron CE, van den Worm SH, Snijder EJ (2009) The RNA polymerase activity of SARS-coronavirus nsp12 is primer dependent. Nucleic Acids Res 38: 203\u2013214.\u00a0\u00a0\u00a0 \u300827. te Velthuis AJ\u3001Arnold JJ\u3001Cameron CE\u3001van den Worm SH\u3001Snijder EJ (2009) SARS \u51a0\u72c0\u75c5\u6bd2 nsp12 \u7684 RNA \u805a\u5408\u9176\u6d3b\u6027\u4f9d\u8cf4\u65bc\u5f15\u7269\u3002\u6838\u9178\u7814\u7a76 38\uff1a203-214\u3002\u3009View Article\u3000Google Scholar 28.Beerens N, Selisko B, Ricagno S, Imbert I, van der Zanden L, et al. (2007) De Novo Initiation of RNA Synthesis by the Arterivirus RNA-Dependent RNA Polymerase. J Virol 81: 8384\u20138395.\u00a0\u00a0\u00a0 \u300828. Beerens N\u3001Selisko B\u3001Ricagno S\u3001Imbert I\u3001van der Zanden L \u7b49\u3002 (2007) \u901a\u904e\u52d5\u8108\u75c5\u6bd2 RNA \u4f9d\u8cf4\u7684 RNA \u805a\u5408\u9176\u5f9e\u982d\u958b\u59cb\u5408\u6210 RNA\u3002 J Virol 81\uff1a8384\u20138395\u3002\u3009View Article\u3000Google Scholar 29.van den Born E, Posthuma CC, Knoops K, Snijder EJ (2007) An infectious recombinant equine arteritis virus expressing green fluorescent protein from its replicase gene. J Gen Virol 88: 1196\u20131205.\u00a0\u00a0\u00a0 \u300829. van den Born E, Posthuma CC, Knoops K, Snijder EJ (2007) \u4e00\u7a2e\u50b3\u67d3\u6027\u91cd\u7d44\u99ac\u52d5\u8108\u708e\u75c5\u6bd2\uff0c\u5f9e\u5176\u8907\u5236\u9176\u57fa\u56e0\u8868\u9054\u7da0\u8272\u7192\u5149\u86cb\u767d\u3002 J Gen Virol 88\uff1a1196-1205\u3002\u3009View Article\u3000Google Scholar 30.Sims AC, Burkett SE, Yount B, Pickles RJ (2008) SARS-CoV replication and pathogenesis in an in vitro model of the human conducting airway epithelium. Virus Res 133: 33\u201344.\u00a0\u00a0\u00a0 \u300830. Sims AC\u3001Burkett SE\u3001Yount B\u3001Pickles RJ (2008) SARS-CoV \u5728\u4eba\u985e\u50b3\u5c0e\u6c23\u9053\u4e0a\u76ae\u9ad4\u5916\u6a21\u578b\u4e2d\u7684\u8907\u5236\u548c\u767c\u75c5\u6a5f\u5236\u3002\u75c5\u6bd2\u7814\u7a76 133\uff1a33-44\u3002\u3009View Article\u3000Google Scholar 31.Xu X, Liu Y, Weiss S, Arnold E, Sarafianos SG, et al. (2003) Molecular model of SARS coronavirus polymerase: implications for biochemical functions and drug design. Nucleic Acids Res 31: 7117\u20137130.\u00a0\u00a0\u00a0 \u300831. Xu X\u3001Liu Y\u3001Weiss S\u3001Arnold E\u3001Sarafianos SG \u7b49\u3002 (2003) SARS \u51a0\u72c0\u75c5\u6bd2\u805a\u5408\u9176\u7684\u5206\u5b50\u6a21\u578b\uff1a\u5c0d\u751f\u5316\u529f\u80fd\u548c\u85e5\u7269\u8a2d\u8a08\u7684\u5f71\u97ff\u3002\u6838\u9178\u7814\u7a76 31\uff1a7117-7130\u3002\u3009View Article\u3000Google Scholar 32.Zhai Y, Sun F, Li X, Pang H, Xu X, et al. (2005) Insights into SARS-CoV transcription and replication from the structure of the nsp7-nsp8 hexadecamer. Nat Struct Mol Biol 12: 980\u2013986.\u00a0\u00a0\u00a0 \u300832. \u7fdf\u6bc5\u3001\u5b6b\u98db\u3001\u674e\u65ed\u3001\u9f90\u83ef\u3001\u5f90\u65ed\u7b49\u3002 (2005) \u5f9e nsp7-nsp8 \u5341\u516d\u805a\u9ad4\u7684\u7d50\u69cb\u6d1e\u5bdf SARS-CoV \u8f49\u9304\u548c\u5fa9\u5236\u3002 Nat Struct Mol Biol 12\uff1a980-986\u3002\u3009View Article\u3000Google Scholar 33.Stockman LJ, Bellamy R, Garner P (2006) SARS: Systematic Review of Treatment Effects. PLoS Med 3: e343.\u00a0\u00a0\u00a0 \u300833. Stockman LJ\u3001Bellamy R\u3001Garner P (2006) SARS\uff1a\u6cbb\u7642\u6548\u679c\u7684\u7cfb\u7d71\u8a55\u50f9\u3002 PLoS Med 3: e343\u3002\u3009View Article\u3000Google Scholar 34.Thompson A, Patel K, Tillman H, McHutchison JG (2009) Directly acting antivirals for the treatment of patients with hepatitis C infection: A clinical development update addressing key future challenges. J Hepatol 50: 184\u2013194.\u00a0\u00a0\u00a0 \u300834. Thompson A\u3001Patel K\u3001Tillman H\u3001McHutchison JG (2009) \u7528\u65bc\u6cbb\u7642\u4e19\u578b\u809d\u708e\u611f\u67d3\u60a3\u8005\u7684\u76f4\u63a5\u6297\u75c5\u6bd2\u85e5\u7269\uff1a\u61c9\u5c0d\u672a\u4f86\u95dc\u9375\u6311\u6230\u7684\u81e8\u5e8a\u958b\u767c\u66f4\u65b0\u3002 J Hepatol 50\uff1a184-194\u3002\u3009View Article\u3000Google Scholar 35.De Clercq E (2004) Antivirals and antiviral strategies. Nat Rev Microbiol 2: 704\u2013720.\u00a0\u00a0\u00a0 \u300835. De Clercq E (2004) \u6297\u75c5\u6bd2\u85e5\u7269\u548c\u6297\u75c5\u6bd2\u7b56\u7565\u3002 Nat Rev Microbiol 2: 704\u2013720\u3002\u3009View Article\u3000Google Scholar 36.Thompson AJV, McHutchison JG (2009) Antiviral resistance and specifically targeted therapy for HCV (STAT-C). J Viral Hepat 16: 377\u2013387.\u00a0\u00a0\u00a0 \u300836. Thompson AJV, McHutchison JG (2009) HCV \u6297\u75c5\u6bd2\u8010\u85e5\u6027\u548c\u7279\u7570\u6027\u9776\u5411\u6cbb\u7642 (STAT-C)\u3002 J \u75c5\u6bd2\u6027\u809d\u75c5 16\uff1a377-387\u3002\u3009View Article\u3000Google Scholar 37.Yang W, Lee JY, Nowotny M (2006) Making and Breaking Nucleic Acids: Two-Mg2+-Ion Catalysis and Substrate Specificity. Mol Cell 22: 5\u201313.\u00a0\u00a0\u00a0 \u300837. Yang W\u3001Lee JY\u3001Nowotny M (2006) \u88fd\u9020\u548c\u7834\u58de\u6838\u9178\uff1a\u96d9 Mg2+ \u96e2\u5b50\u50ac\u5316\u548c\u5e95\u7269\u7279\u7570\u6027\u3002\u6469\u723e\u7d30\u80de 22\uff1a5-13\u3002\u3009View Article\u3000Google Scholar 38.Castro C, Smidansky E, Maksimchuk KR, Arnold JJ, Korneeva VS, et al. (2007) Two proton transfers in the transition state for nucleotidyl transfer catalyzed by RNA- and DNA-dependent RNA and DNA polymerases. Proc Natl Acad Sci USA 104: 4267\u20134272.\u00a0\u00a0\u00a0 \u300838. Castro C\u3001Smidansky E\u3001Maksimchuk KR\u3001Arnold JJ\u3001Korneeva VS \u7b49\u3002 (2007) \u7531 RNA \u548c DNA \u4f9d\u8cf4\u6027 RNA \u548c DNA \u805a\u5408\u9176\u50ac\u5316\u7684\u6838\u82f7\u9178\u8f49\u79fb\u7684\u5169\u500b\u904e\u6e21\u614b\u8cea\u5b50\u8f49\u79fb\u3002 Proc Natl Acad Sci USA 104\uff1a4267\u20134272\u3002\u3009View Article\u3000Google Scholar 39.Arnold JJ, Ghosh SK, Cameron CE (1999) Poliovirus RNA-dependent RNA Polymerase (3Dpol). Divalent cation modulation of primer, template and nucleotide selection. J Biol Chem 274: 37060\u201337069.\u00a0\u00a0\u00a0 \u300839. Arnold JJ\u3001Ghosh SK\u3001Cameron CE (1999) \u810a\u9ad3\u7070\u8cea\u708e\u75c5\u6bd2 RNA \u4f9d\u8cf4\u6027 RNA \u805a\u5408\u9176 (3Dpol)\u3002\u5f15\u7269\u3001\u6a21\u677f\u548c\u6838\u82f7\u9178\u9078\u64c7\u7684\u4e8c\u50f9\u967d\u96e2\u5b50\u8abf\u7bc0\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 274\uff1a37060-37069\u3002\u3009View Article\u3000Google Scholar 40.Iuchi S (2001) Three classes of C2H2 zinc finger proteins. Cell Mol Life Sci 58: 625\u2013635.\u00a0\u00a0\u00a0 \u300840. Iuchi S (2001) \u4e09\u985e C2H2 \u92c5\u6307\u86cb\u767d\u3002\u7d30\u80de\u5206\u5b50\u751f\u547d\u79d1\u5b78 58\uff1a625-635\u3002\u3009View Article\u3000Google Scholar 41.Gomis-Ruth XF (2009) Catalytic domain architecture of metzincin metalloproteases. J Biol Chem 284: 15353\u201315357.\u00a0\u00a0\u00a0 \u300841. Gomis-Ruth XF (2009) Metzincin \u91d1\u5c6c\u86cb\u767d\u9176\u7684\u50ac\u5316\u57df\u7d50\u69cb\u3002\u751f\u7269\u5316\u5b78\u96dc\u8a8c 284\uff1a15353\u201315357\u3002\u3009View Article\u3000Google Scholar 42.Yap TL, Xu T, Chen Y-L, Malet H, Egloff M-P, et al. (2007) Crystal Structure of the Dengue Virus RNA-Dependent RNA Polymerase Catalytic Domain at 1.85-Angstrom Resolution. J Virol 81: 4753\u20134765.\u00a0\u00a0\u00a0 \u300842. Yap TL\u3001Xu T\u3001Chen Y-L\u3001Malet H\u3001Egloff M-P \u7b49\u3002 (2007) \u5206\u8fa8\u7387\u70ba 1.85 \u57c3\u7684\u767b\u9769\u71b1\u75c5\u6bd2\u4f9d\u8cf4\u65bc RNA \u7684 RNA \u805a\u5408\u9176\u50ac\u5316\u7d50\u69cb\u57df\u7684\u6676\u9ad4\u7d50\u69cb\u3002 J Virol 81\uff1a4753\u20134765\u3002\u3009View Article\u3000Google Scholar 43.Winek CL, Buehler EV (1966) Intravenous toxicity of zinc pyridinethione and several zinc salts. Toxicol Appl Pharmacol 9: 296\u2013273.\u00a0\u00a0\u00a0 \u300843. Winek CL, Buehler EV (1966) \u5421\u5576\u786b\u916e\u92c5\u548c\u5e7e\u7a2e\u92c5\u9e7d\u7684\u975c\u8108\u6bd2\u6027\u3002 Toxicol Appl Pharmacol 9\uff1a296-273\u3002\u3009View Article\u3000Google Scholar 44.Snyder DR, de Jesus CP, Towfighi J, Jacoby RO, Wedig JH (1979) Neurological, microscopic and enzyme-histochemical assessment of zinc pyrithione toxicity. Food Cosmet Toxicol 17: 651\u2013660.\u00a0\u00a0\u00a0 \u300844. Snyder DR, de Jesus CP, Towfighi J, Jacoby RO, Wedig JH (1979) \u5421\u5576\u786b\u916e\u92c5\u6bd2\u6027\u7684\u795e\u7d93\u5b78\u3001\u986f\u5fae\u93e1\u548c\u9176\u7d44\u7e54\u5316\u5b78\u8a55\u4f30\u3002\u98df\u54c1\u5316\u599d\u54c1\u6bd2\u7406\u5b78 17\uff1a651-660\u3002\u3009View Article\u3000Google Scholar 45.Magda D, Lecane P, Wang Z, Hu W, Thiemann P, et al. (2008) Synthesis and anticancer properties of water-soluble zinc ionophores. Cancer Res 68: 5318\u20135325.\u00a0\u00a0\u00a0 \u300845. Magda D\u3001Lecane P\u3001Wang Z\u3001Hu W\u3001Thiemann P \u7b49\u3002 (2008) \u6c34\u6eb6\u6027\u92c5\u96e2\u5b50\u8f09\u9ad4\u7684\u5408\u6210\u548c\u6297\u764c\u7279\u6027\u3002\u764c\u75c7\u7814\u7a76 68\uff1a5318-5325\u3002\u3009View Article\u3000oogle Scholar 46.Snijder EJ, van der Meer Y, Zevenhoven-Dobbe J, Onderwater JJM, van der Meulen J, et al. (2006) Ultrastructure and Origin of Membrane Vesicles Associated with the Severe Acute Respiratory Syndrome Coronavirus Replication Complex. J Virol 80: 5927\u20135940.\u00a0\u00a0\u00a0 \u300846. Snijder EJ\u3001van der Meer Y\u3001Zevenhoven-Dobbe J\u3001Onderwater JJM\u3001van der Meulen J \u7b49\u3002 (2006) \u8207\u56b4\u91cd\u6025\u6027\u547c\u5438\u7cfb\u7d71\u7d9c\u5408\u75c7\u51a0\u72c0\u75c5\u6bd2\u8907\u88fd\u8907\u5408\u9ad4\u76f8\u95dc\u7684\u819c\u56ca\u6ce1\u7684\u8d85\u5fae\u7d50\u69cb\u548c\u8d77\u6e90\u3002 J Virol 80\uff1a5927-5940\u3002\u3009View Article\u3000Google Scholar 47.Studier F (2005) Protein production by auto-induction in high density shaking cultures. Protein Expr Purif 41: 207\u2013234.\u00a0\u00a0\u00a0 \u300847. Studier F (2005) \u5728\u9ad8\u5bc6\u5ea6\u632f\u76ea\u57f9\u990a\u4e2d\u901a\u904e\u81ea\u52d5\u8a98\u5c0e\u7522\u751f\u86cb\u767d\u8cea\u3002\u86cb\u767d\u8cea Expr Purif 41\uff1a207-234\u3002\u3009View Article\u3000Google Scholar   \u91cd\u5fb7\u4e26\u4e14\u5c07\u5728\u5317\u4eac\u7684\u300c\u4e2d\u570b\u79d1\u5b78\u9662\u751f\u7269\u7269\u7406\u7814\u7a76\u6240\u611f\u67d3\u8207\u514d\u75ab\u5b78\u91cd\u9ede\u5be6\u9a57\u5ba4\u300d\u5728\u91ab\u5b78\u96dc\u8a8c\u4e0a\u6240\u767c\u8868\u7684\u4e00\u7bc7\u82f1\u6587\u7814\u7a76\u5831\u544a\u300a\u92c5\u6307\u6297\u75c5\u6bd2\u86cb\u767d\u6291\u5236\u7532\u578b\u6d41\u611f\u75c5\u6bd2\u86cb\u767d\u8868\u9054\u4ee5\u53ca\u88ab\u75c5\u6bd2\u7de8\u78bcNS1 \u5c0d\u6297\u7684\u7c21\u8981\u5f62\u5f0f\u300b\u7684\u4e2d\u6587\u81ea\u8b6f\u7248\u4e5f\u5bc4\u4e0a\u7d66\u4ed6\u53c3\u8003(\u5982\u9644\u6a94)\u3002 \u4e26\u4e14\uff0c\u91cd\u5fb7\u9084\u767c\u53bb\u4ee5\u4e0b\u5716\u7247\u8207\u8aaa\u660e\uff1a   \u300e\u9019\u5c31\u662f\u92c5\u6307\u7d50\u69cb\u5716\uff0c\u7531\u65bc\u9019\u662f\u87af\u5408\u7d50\u69cb\uff0c\u56e0\u6b64\u5b83\u80fd\u5f88\u5bb9\u6613\u5c07\u92c5\u96e2\u5b50\u7a7f\u900f\u7d30\u80de\u58c1\u800c\u8207\u8eb2\u85cf\u5728\u7d30\u80de\u5167\u840c\u767c\u7684\u75c5\u6bd2\u56e0\u70ba\u767c\u751f\u6291\u5236\u7ffb\u8b6f\u548c\u4fc3\u9032\u75c5\u6bd2 mRNA\u7684\u964d\u89e3\uff0c\u56e0\u6b64\u901a\u904e PARP \u7d50\u69cb\u57df\u800c\u4fc3\u9032 IAV \u86cb\u767d\u964d\u89e3\uff0c\u5be6\u9a57\u5df2\u7d93\u8b49\u5be6\u5b83\u53ef\u8b93\u5165\u4fb5\u5230\u7d30\u80de\u5167\u7684\u75c5\u6bd2\u4e4bRNA\u6a5f\u8f49\u767c\u751f\u4f11\u514b(\u4f46\u4e0d\u4e00\u5b9a\u6703\u6b7b\u4ea1)\uff0c\u800c\u4f7f\u5f97\u539f\u672c\u7121\u751f\u547d\u7684\u75c5\u6bd2\u56e0\u6b64\u4e0d\u80fd\u5927\u91cf\u8907\u88fd\u840c\u751f\uff0c\u800c\u53ef\u4ee5\u70ba\u4eba\u9ad4\u7684\u514d\u75ab\u7cfb\u7d71\u89e3\u6c7a\u3002\u300f \u9019\u4e00\u4f4d\u738b\u59d3\u7db2\u53cb\u53c8\u767c\u4f86\u4ee5\u4e0b\u4fe1\u606f\uff1a\u300e\u80fd\u5920\u6bba\u75c5\u6bd2\u800c\u4e0d\u6703\u6bba\u6b7b\u6211\u5011\u81ea\u5df1\u7684\u7d30\u80de,\u5929\u4e0b\u6709\u9019\u9ebc\u597d\u7684\u4e8b\u55ce\uff1f\u4e0d\u904e\u662f\u71df\u990a\u88dc\u52a9\u54c1\u800c\u5df2\uff01\u300f \u91cd\u5fb7\u770b\u4ed6\u5728\u610f\u6c23\u7528\u4e8b\uff0c\u56e0\u6b64\u5c31\u56de\u8986\u4ed6\u8aaa\uff1a\u300e\u96a8\u60a8\u9ad8\u8208\u600e\u9ebc\u8aaa\u90fd\u597d\uff0c\u6211\u6c92\u6709\u8aaa\u5b83\u662f\u85e5\uff0c\u53ea\u8aaa\u662f\u4fdd\u5065\u54c1\u800c\u5df2\u3002\u300f\u767c\u5b8c\u4e86\u9019\u4fe1\u606f\u4e4b\u5f8c\uff0c\u91cd\u5fb7\u767c\u8a93\u4ee5\u5f8c\u518d\u4e5f\u4e0d\u5c0d\u9019\u7a2e\u96de\u86cb\u88e1\u6311\u9aa8\u982d\u7684\u4eba\u63d0\u4f9b\u4efb\u4f55\u6709\u50f9\u503c\u7684\u8cc7\u8a0a\u4e86\u3002 \u4f46\u662f\uff0c\u91cd\u5fb7\u9084\u662f\u8981\u544a\u8a34\u5927\u5bb6\u70ba\u4f55\u8981\u5efa\u8b70\u5927\u5bb6\u5403\u542b\u92c5\u96e2\u5b50\u7684\u4fdd\u5065\u98df\u54c1\uff0c\u7576\u7136\u92c5\u5f9e\u98df\u7269\u4e2d\u651d\u53d6\u4e5f\u6709\uff0c\u4f46\u662f\u98df\u7269\u5fc5\u9808\u7d93\u904e\u6d88\u5316\u5f8c\u92c5\u96e2\u5b50\u624d\u6703\u900f\u904e\u8840\u6db2\u800c\u5230\u4eba\u9ad4\u5668\u5b98\uff0c\u9019\u500b\u904e\u7a0b\u592a\u9577\u592a\u4e45\u4e86\uff0c\u56e0\u6b64\u92c5\u96e2\u5b50\u53ef\u80fd\u53c8\u8207\u5176\u4ed6\u7269\u8cea\u7d50\u5408\u6210\u5316\u5408\u7269\uff0c\u800c\u518d\u4e0d\u80fd\u767c\u751f\u8207\u8ca0\u96e2\u5b50\u7684\u75c5\u6bd2\u4e2d\u548c\u7684\u6548\u679c\u3002\u56e0\u6b64\u5c31\u66f4\u6c92\u6709\u767c\u63ee\u5230\u92c5\u96e2\u5b50\u6240\u7368\u5177\u7684\u87af\u5408\u4ea4\u63db\u6548\u679c\u3002 \u7531\u65bc\uff0c\u611f\u5192\u6216\u662fCOVID-19\u7684\u521d\u671f\u75c5\u72c0\u4e3b\u8981\u90fd\u662f\u91dd\u5c0d\u4e0a\u652f\u6c23\u7ba1\uff0c\u56e0\u6b64\u4ee3\u8868\u547c\u5438\u7ba1\u9053\u7684\u75c5\u6bd2\u91cf\u6700\u5927\uff0c\u56e0\u6b64\u53ef\u4ee5\u542b\u5728\u5634\u4e2d\u6162\u6162\u5206\u89e3\u7684\u5589\u7cd6\u4e4b\u6548\u679c\u6700\u597d\uff0c\u56e0\u70ba\u5b83\u8b93\u901a\u904e\u7684\u6c23\u6d41\u4e2d\u7684\u75c5\u6bd2\u5148\u88ab\u92c5\u96e2\u5b50\u4e2d\u548c\uff0c\u800c\u7cd6\u6c41\u6d41\u5165\u98df\u9053\u6642\u92c5\u96e2\u5b50\u4e5f\u6703\u6ea2\u5165\u4e0a\u652f\u6c23\u7ba1\u3002\u9019\u5c31\u662f\u70ba\u4f55\u91cd\u5fb7\u9996\u5148\u63a8\u85a6\u5df2\u7d93\u5728\u7f8e\u570b\u98a8\u884c30\u591a\u5e74\u7684\u611f\u5192\u9810\u9632\u7528\u85e5Cold Eeze\u4e2d\u6587\u8b6f\u540d\u70ba\u3010\u92c5\u7cd6\u3011\uff0c\u56e0\u70ba\u5b83\u5df2\u7d93\u904e\u4e86\u5c08\u5229\u4fdd\u8b77\u671f\uff0c\u5b78\u540d\u85e5\u540d\u662fGeneric Version\uff0c\u56e0\u70ba\u552e\u50f9\u8f03\u4fbf\u5b9c(\u4f46\u662f\u73fe\u5728\u5168\u7403\u6436\u8cfc\u5f88\u53ef\u80fd\u7f3a\u8ca8)\u3002 \u73fe\u5728\uff0c\u91cd\u5fb7\u8981\u544a\u8a34\u5927\u5bb6\u4e00\u500b\u79d8\u5bc6\uff0c\u90a3\u5c31\u662f\u70ba\u4f55\u91cd\u5fb7\u8981\u5c0d\u92c5\u96e2\u5b50\u9032\u884c\u7814\u7a76\u5462\uff0c\u6545\u4e8b\u662f\u9019\u6a23\u5b50\u7684\uff0c\u7531\u65bc\u4e00\u5e74\u524d \u91cd\u5fb7 \u8acb\u793a\u201c\u9748\u7236\u201d(\u5143\u59cb\u5929\u5c0a)\u8aaa\uff1a\u300e\u65e2\u7136\u00a0 \u9748\u7236\u80fd\u63d0\u524d\u534a\u5e74\u8b66\u544a\u6211\u5011\u5c07\u6709\u6b64\u5927\u75ab\u60c5\uff0c\u662f\u5426\u80fd\u544a\u77e5\u5f92\u5152\u5982\u4f55\u88fd\u5f97\u89e3\u85e5\uff1f\u300f\u64f2\u832d\u61c9\u8a31\u5f8c\uff0c\u91cd\u5fb7 \u6b63\u4e0d\u77e5\u5982\u4f55\u518d\u554f\u9019\u89e3\u85e5\u79d8\u65b9\u6642\uff0c\u8166\u4e2d\u7a81\u7136\u6e67\u73fe\u4e00\u500b\u201c\u92c5\u201d\u5b57\uff0c\u64f2\u832d\u518d\u554f\u78ba\u5b9a\u795e\u8aed\u5c31\u662f\u6b64\u201c\u92c5\u201d\u5b57\u7121\u8aa4\u3002 \u5728\u6b64\u4e4b\u5f8c\uff0c\u91cd\u5fb7\u624d\u958b\u59cb\u8a73\u95b1\u5404\u7a2e\u82f1\u6587\u91ab\u5b78\u6587\u737b\uff0c\u8d8a\u767c\u8b49\u5be6\u92c5\u96e2\u5b50\u6709\u6291\u5236mRNA\u75c5\u6bd2\u7684\u6548\u679c\uff0c\u800c\u4e14\u56e0\u70ba\u92c5\u96e2\u5b50\u662f\u63a1\u53d6\u87af\u5408\u65b9\u5f0f\u8b93\u92c5\u96e2\u5b50\u7a7f\u900f\u7d30\u80de\u58c1\u9032\u5165\u4f86\u964d\u89e3\u75c5\u6bd2\u86cb\u767d\u8907\u88fd\u6240\u9700\u6c42\u7684PARP \u7d50\u69cb\u57df\uff0c\u56e0\u6b64\u9019\u662f\u4e00\u7a2e\u7cbe\u6e96\u7834\u7a97\u5f0f\u7684\u65ac\u9996\u884c\u52d5\uff0c\u5c0d\u65bc\u7d30\u80de\u7684\u50b7\u5bb3\u751a\u5c0f\uff0c\u56e0\u6b64\u5c0d\u65bc\u4eba\u9ad4\u7684\u5371\u5bb3\u751a\u4f4e\u3002 \u91cd\u5fb7\u4e00\u5e74\u524d\u5c31\u628a\u7814\u7a76\u6210\u679c\u516c\u958b\uff0c\u4e00\u4f86\u56e0\u70ba\u81ea\u5df1\u962e\u56ca\u7f9e\u6f80\uff0c\u7121\u6cd5\u7e7c\u7e8c\u7814\u767c\uff0c\u4e8c\u4f86\u91cd\u5fb7\u5df2\u7d93\u9810\u7aba\u5230\u672a\u4f86\u9084\u6709\u5176\u4ed6\u56b4\u91cd\u75be\u75c5\uff0c\u800c\u4e14COVID-19\u9084\u6703\u5927\u7a81\u8b8a\uff0c\u56e0\u6b64\u8b93\u5176\u4ed6\u4eba\u63a5\u68d2\u4f86\u7814\u7a76\u6216\u8a31\u6703\u66f4\u6709\u6210\u6548\u3002","breadcrumb":{"@id":"https:\/\/pingtungtimes.com.tw\/?p=104514#breadcrumb"},"inLanguage":"zh-TW","potentialAction":[{"@type":"ReadAction","target":["https:\/\/pingtungtimes.com.tw\/?p=104514"]}]},{"@type":"ImageObject","inLanguage":"zh-TW","@id":"https:\/\/pingtungtimes.com.tw\/?p=104514#primaryimage","url":"https:\/\/pingtungtimes.com.tw\/wp-content\/uploads\/2022\/05\/\u4e00\u822c\u4eba\u5f9e\u98df\u7269\u4e2d\u651d\u53d6\u92c5\u70ba\u7684\u5c31\u662f\u58ef\u967d\u8207\u589e\u9aee.jpg","contentUrl":"https:\/\/pingtungtimes.com.tw\/wp-content\/uploads\/2022\/05\/\u4e00\u822c\u4eba\u5f9e\u98df\u7269\u4e2d\u651d\u53d6\u92c5\u70ba\u7684\u5c31\u662f\u58ef\u967d\u8207\u589e\u9aee.jpg","width":897,"height":468,"caption":"\u25bc\u4e00\u822c\u4eba\u5f9e\u98df\u7269\u4e2d\u651d\u53d6\u201c\u92c5\u201d\u70ba\u7684\u5c31\u662f\u58ef\u967d\u8207\u589e\u9aee\u3002\uff08\u5716\uff0f\u674e\u91cd\u5fb7\u63d0\u4f9b\uff09"},{"@type":"BreadcrumbList","@id":"https:\/\/pingtungtimes.com.tw\/?p=104514#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"\u9996\u9801","item":"https:\/\/pingtungtimes.com.tw\/"},{"@type":"ListItem","position":2,"name":"\u597d\u201c\u92c5\u201d\u3000\u4e5f\u6703\u60f9\u4e86\u4e00\u8eab\u8165"}]},{"@type":"WebSite","@id":"https:\/\/pingtungtimes.com.tw\/#website","url":"https:\/\/pingtungtimes.com.tw\/","name":"\u5c4f\u6771\u6642\u5831\u96fb\u5b50\u5831","description":"\u5c4f\u6771\u6642\u5831\u96fb\u5b50\u5831PingtungTimes\uff0c\u6700\u95dc\u5fc3\u5728\u5730\u7684\u5a92\u9ad4","publisher":{"@id":"https:\/\/pingtungtimes.com.tw\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/pingtungtimes.com.tw\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"zh-TW"},{"@type":"Organization","@id":"https:\/\/pingtungtimes.com.tw\/#organization","name":"\u5c4f\u6771\u6642\u5831\u96fb\u5b50\u5831","url":"https:\/\/pingtungtimes.com.tw\/","logo":{"@type":"ImageObject","inLanguage":"zh-TW","@id":"https:\/\/pingtungtimes.com.tw\/#\/schema\/logo\/image\/","url":"https:\/\/pingtungtimes.com.tw\/wp-content\/uploads\/2023\/02\/logo-150.png","contentUrl":"https:\/\/pingtungtimes.com.tw\/wp-content\/uploads\/2023\/02\/logo-150.png","width":150,"height":150,"caption":"\u5c4f\u6771\u6642\u5831\u96fb\u5b50\u5831"},"image":{"@id":"https:\/\/pingtungtimes.com.tw\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/www.facebook.com\/pingtungnews"]},{"@type":"Person","@id":"https:\/\/pingtungtimes.com.tw\/#\/schema\/person\/90cf880d4d210d8db447ae0199435aa4","name":"fanjiang","image":{"@type":"ImageObject","inLanguage":"zh-TW","@id":"https:\/\/secure.gravatar.com\/avatar\/392bb75508611fa0e892a64a45ae50b3be151c3f249f4fc2e86107740441ff2b?s=96&d=blank&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/392bb75508611fa0e892a64a45ae50b3be151c3f249f4fc2e86107740441ff2b?s=96&d=blank&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/392bb75508611fa0e892a64a45ae50b3be151c3f249f4fc2e86107740441ff2b?s=96&d=blank&r=g","caption":"fanjiang"},"url":"https:\/\/pingtungtimes.com.tw\/?author=1"}]}},"_links":{"self":[{"href":"https:\/\/pingtungtimes.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/104514","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pingtungtimes.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/pingtungtimes.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/pingtungtimes.com.tw\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/pingtungtimes.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=104514"}],"version-history":[{"count":0,"href":"https:\/\/pingtungtimes.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/104514\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pingtungtimes.com.tw\/index.php?rest_route=\/wp\/v2\/media\/104516"}],"wp:attachment":[{"href":"https:\/\/pingtungtimes.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=104514"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/pingtungtimes.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=104514"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/pingtungtimes.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=104514"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}